• Natural Product Sciences
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• 제23권2호
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• pp.125-131
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• 2017

Clitoria ternatea or Commonly known blue pea, is a perennial climber crop native to Asian countries. The current study was aimed to evaluate the antimicrobial activity C. ternatea extract on food borne microorganisms and its antifungal effect on Penicillium expansum. The extract showed significant antimicrobial activity against 3 Gram positive bacteria, 2 Gram negative bacteria and 1 filamentous fungus on disc diffusion assay. The extract also showed good biocidal effect on all Gram positive bacteria tested and P. expansum. However, the kill curve analysis revealed that the fungicidal activity of the extract against P. expansum conidia was depend on the concentration of the extract and the time of exposure of the conidia to the extract. The scanning electron micrograph of the extract treated P. expansum culture showed alterations in the morphology of fungal hyphae. The germination of P. expansum conidia was completely inhibited and conidial development was totally suppressed by the extract, suggesting the possible mode of action of anthocyanin. Besides, the extract also exhibited 5.0-log suppression of microbial growth relative to control in the rice model. The results indicate the potential use of the C. ternatea anthocyanin as food biopreservative.

• The Plant Pathology Journal
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• 제25권1호
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• pp.38-46
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• 2009

Lichen-forming fungi (LEF) were isolated from 67 Hungarian lichen species from ascospores or thallus fragments. LFF were successfully isolated from 26 species with isolation rate of 38.8%. Of the total number of isolation from ascospores (27 species) and thallus fragments (40 species), 48% and 32.5% of the species were successfully isolated, respectively. Comparison of rDNA sequences of ITS regions between the isolated LFF and the original thallus confirmed that all the isolates originated from the thallus fragments were LEF. The following 14 species of LEF were newly isolated in this study; Acarospora cervina, Bacidia rubella, Cladonia pyxidata, Lasallia pustulata, Lecania hyaline, Lecanora argentata, Parmelina tiliacea, Parmotrema chinense, Physconia distorta, Protoparmeliopsis muralis, Ramalina pollinaria, Sarcogyne regularis, Umbilicaria hirsuta, Xanthoparmelia conspersa and X. stenophylla. Antifungal activity of the Hungarian LFF was evaluated against plant pathogenic fungi of Colletotrichum acutatum, C. coccodes and C. gloeosporioides, causal agent of anthracnose on hot pepper. Among the 26 isolates, 11 LFF showed more than 50% of inhibition rates of mycelial growth of at least one target pathogen. Especially, LFF of Evernia prunastri, Lecania hyalina and Lecanora argentata were remarkably effective in inhibition of mycelial growth of all the tested pathogens with antibiotic mode of action. On the other hands, five isolates of Cladonia furcata, Hypogymnia physodes, Lasallia pustulata, Ramalina fastigiata and Ramalina pollinaria exhibited fungal lytic activity against all the three pathogens. Among the tested fungal pathogens, C. coccodes seemed to be most sensitive to the LFF. The Hungarian LFF firstly isolated in this study can be served as novel bioresources to develop new biofungicides alternative to current fungicides to control hot pepper anthracnose pathogenic fungi.

• 한국전자파학회논문지
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• 제21권1호
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• pp.53-60
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• 2010

본 논문에서는 5.2 GHz에서 입력 신호의 크기에 따라 효율적으로 동작하는 저잡음 증폭기를 0.18 um CMOS 공정을 이용하여 설계하였다. 제안된 회로는 궤환 회로와 2단 저잡음 증폭기로 구성되어 있으며, 궤환 회로의 경우 7개의 함수 블록으로 구성되어 있다. 본 논문에서는 변화되는 신호 전압을 감지하는 것과 이전 상태를 기억하는 저장 회로에 초점을 두어 불필요한 전력 소비를 제거하였다. 기억 기능 특성을 갖는 궤환 회로의 출력값을 이용하여 통제되는 저잡음 증폭기는 11.39 dB에서 22.74 dB까지 변하며, 최고 이득 모드일 때 잡음 지수가 최적화 되도록 설계되었다. 변환 저잡음 증폭기는 1.8 V의 공급 전압에 대해서 5.68~6.75 mW를 소비한다.

• 한국조명전기설비학회지:조명전기설비
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• 제7권6호
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• pp.27-33
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• 1993

본 논문에서는 몬테카를로 시뮬레이션을 이용하여 복잡한 다면 실내공간에서 조도분포를 예측하는 방법을 제안하였다. 광원으로부터 방사하는 빛을 배광분포에 따른 가중치를 가지고 진행하는 입자로 가정하였으며 광원에서 방출된 빛의 경로를 추적하여 반사면과의 교점을 구한다. 그리고 반사면의 반사율을 고려하여 이자가 반사 또는 흡수될 것인가를 판단하고 그 입자가 흡수될 때까지 입자의 진행을 반복한다. 무수히 많은 입자에 대해 위와 같은 과정을 반복하면 실내면의 조도분포를 얻을 수 있다. 보다 세밀한 측정을 위하여 각 실내면은 매우 작은 미소면적으로 분할하였다. 그리고, 실제공간에서도 응용될 수 있도록 실제의 공간과 유사한 다면 실내공간에 대해서 시뮬레이션을 하였으며, 조도분포에 따라 이차원의 등조도 곡선과 삼차원의 조도분포곡선을 그렸다. 실제로 모델공간을 제작하여 조도를 측정해 본 결과, 실험치와 계산치의 오차가 평균 2.3% 이내로 되었다.

• The Plant Pathology Journal
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• 제27권3호
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• pp.207-224
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• 2011

Diseases caused by plant pathogenic bacteria constitute an emerging threat to global food security. Xanthomonas is a large genus of Gram-negative bacteria that cause disease in several host plants leading to considerable losses in productivity and quality of harvests. Despite the ranges of controlling techniques available, the microbiological safety of economically important crops and crop plants including fruits and vegetables continues to be a major concern to the agriculture industry. On the other hand, many of the currently available antimicrobial agents for agriculture are highly toxic, non-biodegradable and cause extended environmental pollution. Besides, the use of antibiotics has provoked an increased resistance among the bacterial pathogens and their pathovars. Thus, novel efficient and safe remedies for controlling plant bacterial diseases are necessary. There has been an increasing interest worldwide on therapeutic values of natural products such as essential oils, hence the purpose of this review is to provide an overview of the published data on the antibacterial efficacy of essential oils that could be considered suitable for application in agriculture as biocontrol measures against plant pathogenic bacteria of Xanthomonas species. The current knowledge on the use of essential oils to control Xanthomonas bacteria in vitro and in vivo models has been discussed. A brief description on the legal aspects on the use of essential oils against bacterial pathogens has also been presented. Through this review, a mode of antibacterial action of essential oils along with their chemical nature and the area for future research have been thoroughly discussed.

• The Korean Journal of Physiology and Pharmacology
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• 제16권1호
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• pp.25-30
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• 2012

Under some pathological conditions as bile flow obstruction or liver diseases with the enterohepatic circulation being disrupted, regurgitation of bile acids into the systemic circulation occurs and the plasma level of bile acids increases. Bile acids in circulation may affect the nervous system. We examined this possibility by studying the effects of bile acids on gating of neuronal (N)-type $Ca^{2+}$ channel that is essential for neurotransmitter release at synapses of the peripheral and central nervous system. N-type $Ca^{2+}$ channel currents were recorded from bullfrog sympathetic neuron under a cell-attached mode using 100 mM $Ba^{2+}$ as a charge carrier. Cholic acid (CA, $10^{-6}M$) that is relatively hydrophilic thus less cytotoxic was included in the pipette solution. CA suppressed the open probability of N-type $Ca^{2+}$ channel, which appeared to be due to an increase in (no activity) sweeps. For example, the proportion of sweep in the presence of CA was ~40% at +40 mV as compared with ~8% in the control recorded without CA. Other single channel properties including slope conductance, single channel current amplitude, open and shut times were not significantly affected by CA being present. The results suggest that CA could modulate N-type $Ca^{2+}$ channel gating at a concentration as low as $10^{-6}M$. Bile acids have been shown to activate nonselective cation conductance and depolarize the cell membrane. Under pathological conditions with increased circulating bile acids, CA suppression of N-type $Ca^{2+}$ channel function may be beneficial against overexcitation of the synapses.

• The Korean Journal of Physiology and Pharmacology
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• 제17권2호
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• pp.149-156
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• 2013

Interstitial cells of Cajal (ICCs) are the pacemaker cells in the gastrointestinal tract, and histamine is known to regulate neuronal activity, control vascular tone, alter endothelial permeability, and modulate gastric acid secretion. However, the action mechanisms of histamine in mouse small intestinal ICCs have not been previously investigated, and thus, in the present study, we investigated the effects of histamine on mouse small intestinal ICCs, and sought to identify the receptors involved. Enzymatic digestions were used to dissociate ICCs from small intestines, and the whole-cell patch-clamp configuration was used to record potentials (in current clamp mode) from cultured ICCs. Histamine was found to depolarize resting membrane potentials concentration dependently, and whereas 2-PEA (a selective H1 receptor agonist) induced membrane depolarizations, Dimaprit (a selective H2-agonist), R-alpha-methylhistamine (R-alpha-MeHa; a selective H3-agonist), and 4-methylhistamine (4-MH; a selective H4-agonist) did not. Pretreatment with $Ca^{2+}$-free solution or thapsigargin (a $Ca^{2+}$-ATPase inhibitor in endoplasmic reticulum) abolished the generation of pacemaker potentials and suppressed histamine-induced membrane depolarization. Furthermore, treatments with U-73122 (a phospholipase C inhibitor) or 5-fluoro-2-indolyl des-chlorohalopemide (FIPI; a phospholipase D inhibitor) blocked histamine-induced membrane depolarizations in ICCs. On the other hand, KT5720 (a protein kinase A inhibitor) did not block histamine-induced membrane depolarization. These results suggest that histamine modulates pacemaker potentials through H1 receptor-mediated pathways via external $Ca^{2+}$ influx and $Ca^{2+}$ release from internal stores in a PLC and PLD dependent manner.

• The Korean Journal of Physiology and Pharmacology
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• 제21권1호
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• pp.65-74
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• 2017

Here we investigated the central processing mechanisms of mechanical allodynia and found a direct excitatory link with low-threshold input to nociceptive neurons. Experiments were performed on male Sprague-Dawley rats weighing 230-280 g. Subcutaneous injection of interleukin 1 beta ($IL-1{\beta}$) ($1ng/10{\mu}L$) was used to produce mechanical allodynia and thermal hyperalgesia. Intracisternal administration of bicuculline, a gamma aminobutyric acid A ($GABA_A$) receptor antagonist, produced mechanical allodynia in the orofacial area under normal conditions. However, intracisternal administration of bicuculline (50 ng) produced a paradoxical anti-allodynic effect under inflammatory pain conditions. Pretreatment with resiniferatoxin (RTX), which depletes capsaicin receptor protein in primary afferent fibers, did not alter the paradoxical anti-allodynic effects produced by the intracisternal injection of bicuculline. Intracisternal injection of bumetanide, an Na-K-Cl cotransporter (NKCC 1) inhibitor, reversed the $IL-1{\beta}$-induced mechanical allodynia. In the control group, application of GABA ($100{\mu}M$) or muscimol ($3{\mu}M$) led to membrane hyperpolarization in gramicidin perforated current clamp mode. However, in some neurons, application of GABA or muscimol led to membrane depolarization in the $IL-1{\beta}$-treated rats. These results suggest that some large myelinated $A{\beta}$ fibers gain access to the nociceptive system and elicit pain sensation via $GABA_A$ receptors under inflammatory pain conditions.

• 대한전기학회:학술대회논문집
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• 대한전기학회 2005년도 제36회 하계학술대회 논문집 A
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• pp.277-279
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• 2005

Recently, due to the increase of power conversion devices and nonlinear loads with the development of information, communication and control technologies, the instantaneous minute interruption factors such as voltage & current harmonics, surge occurring frequency, instantaneous voltage variation, voltage unbalance, flicker etc. have greatly threatened the power quality, and the deterioration of electric power facilities and the functional error of controllers are increasing. As such an instantaneous minute interruption appears to be small and local, accurate evaluation with measurement is difficult and total analysis system is required through a wide range of power quality effect analysis such as the simultaneous measurement on various power supply phenomena and the analysis on the interrelation with system loads. Most of conventional power quality diagnosis equipments have beer developed and applied, which were able to measure the stability rate of frequency, the stability rate of voltage, the electricity-failure duration etc, However, they were insufficient to analyze the system present situation, understand the cause of the failure occurred by the problem of power quality and analyze out the phenomena. Accordingly, this study will address the development of the system for a wide range of power quality diagnosis over the present level, the system for supporting the determination such as the analysis on risk factors, failure mode and impact, the system for harmonic evaluation based on international standards(IEC 61000 Series) and the total power quality diagnosis network & system with the extension and openness as a local and national-scale broadband power quality diagnosis system.

• International Journal of Fluid Machinery and Systems
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• 제9권3호
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• pp.245-255
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• 2016

The alternating flow hydraulics (AFH) had demonstrated the unique features in the past. One of the most well-known inventions was the hydraulic machine-gun synchronizer, which had become the standard equipment of airplane during World War I. The studies on the AFH between 1960 and 1980 had trigged many researchers' interests and reached the summit. The disadvantages of the AFH like low efficiency and cooling difficulty had prevented the further development. Few people are engaged in studying the AFH at present. However, the unique characteristics of the AFH inspire the researchers to continuously explore the new special suitable applications. The overviews of the AFH and the new potential application in the geotechnical testing field have been discussed in this paper. First, the research results of the AFH in the past have been summarized. Then, the classifications of the AFH have been introduced in detail according to the working principle, the number of hydraulic transmission pipelines and the mode of input energy. The advantages and the disadvantages of the AFH have been discussed. A novel potential suitable application in the soil test field has been presented at last. The detailed designing ideas of a new dynamic trixial instrument have been given, which will be a more innovational and energy-saving plan according to the current studies. A series of simulation experiments have been done. The simulation results show that the proposed scheme for the new dynamic trixial instrument is feasible. The paper work will also give some inspirations in the reciprocating motion control system.