• 제목/요약/키워드: Culture-independent

검색결과 827건 처리시간 0.023초

기관 없는 신체와 탈영토화 된 패션 사이의 상관관계에 대한 비판적 고찰 - 철학적, 사회학적 신체 담론과 욕망이론을 중심으로 - (A Critical Study about the Correlation between the Body without Organ and Blurring Boundary Fashion - Focusing on Philosophical and Sociological Discourses about the Body and the Desire -)

  • 양희영;이명희
    • 복식문화연구
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    • 제18권5호
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    • pp.824-841
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    • 2010
  • In 21st century, subordinated relationship of fashion from the body has been disappearing, and fashion is understood as variable and creative field. This research aims at analysis about the relationship between the body and fashion depending on the theories about the authority and the desire. So, this utilizes between theoretical study and empirical analysis. For concentrated study, research period limits from 2000 to the present time 2010. Contemporary body and fashion have being changed into various forms and values, become complex and de-territory. Especially, body is symbol of ambivalence eroticism that gives point to sexual property, and the object of fetishism and machine having a desire. This study's purpose draw a parallel with between the limits of contemporary body that couldn't be rid of the capital and desire, and the liberty of fashion that escape from the body's influence has being changed independent and fluid space. This research's results are as follow as; contemporary de-territory fashion is expressed as 1) symbol of the object and physical material property, 2) self-transcendental instrument fashion, 3) independent spatial molding, 4) de-centering fashion.

DGGE 방법과 Pyrosequencing 방법을 이용한 지렁이 장내미생물의 다양성 분석 (Comparative Analysis of Bacterial Diversity in the Intestinal Tract of Earthworm (Eisenia fetida) using DGGE and Pyrosequencing)

  • 김은성;홍성욱;정건섭
    • 한국미생물·생명공학회지
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    • 제39권4호
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    • pp.374-381
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    • 2011
  • 미생물과의 상호작용을 통하여 토양의 특성을 변화시킬 수 있는 지렁이 Eisenia fetida의 장내미생물 군집을 조사하기 위하여, 배양방법과 비배양방법인 DGGE와 pyrosequencing을 이용하여 8주와 16주 사육 지렁이의 장내미생물 군집을 분석하였다. 배양방법에서는 L. fusiformis(51%), B. cereus(30%), E. aerogenes(21%), 그리고 L. sphaericus (15%) 등이 우점미생물로 확인되었다. DGGE 분석에서는 B. cereus(15.1%), Enterobacter sp.(13.6%), uncultured bacterium (13.1%), 그리고 B. stearothermophilus(7.8%)가 우점미생물로 확인되었다. Pyrosequencing 분석에서는 Microbacterium soli(26%), B. cereus(10%), M. esteraromaticum(6%), 그리고 Frigoribacterium sp.(6%)가 우점미생물로 확인되었다. 그외에도 Aeromonas sp., Pseudomonas sp., Borrelia sp., Cellulosimicrobium sp., Klebsiella sp., and Leifsonia sp. 등의 미생물도 확인이 되었으며, 비배양 방법을 이용한 장내 미생물 군집 조사는 배양이 불가능한 미생물을 확인할 수 있을 뿐만 아니라 더 다양한 미생물 군집도 확인할 수 있었다.

출판 플랫폼으로서의 도서관 - 독립출판을 통해 바라본 출판과 도서관의 상생 - (A Library As a Publishing Platform - on collaborative relationship between libraries and publishers -)

  • 정준민
    • 한국도서관정보학회지
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    • 제49권4호
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    • pp.21-51
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    • 2018
  • 출판 산업이 위기를 맞고 있는 가운데 그 활로로 도서관을 상정하였다. 그러나 도서관이 단순히 독서문화진흥의 촉매로서 출판 산업을 부흥시킬 수 없음을 전제하였다. 차라리 독자가 중심이 되고 도서관을 생산기지로 하는 독자들의 적극적인 생산과 수요를 출판이 수용함으로써 출판콘텐츠의 확대 재생산 체제를 출판 진흥의 모델로 제시하였다. 그 과정에 도서관/출판 협업 (협동조합) 형태의 독립출판 플랫폼을 설계하였고 그 유통과 재생산 모형을 제안하였다.

On the Study of Textual Classics and Artistic Creation - Taking Buddhist Art Dunhuang Grottoes as an Example

  • Liu Tingting
    • International Journal of Advanced Culture Technology
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    • 제11권3호
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    • pp.205-210
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    • 2023
  • Stone cave paintings are continuous interactions as independent mediums in places such as text, images and stone cave architecture. Unlike Buddha statues, the narrative of the text always fascinates and guides the viewer to the timeliness of the image, that is, the narrative. In particular, in Buddhist art, Buddha statues are never simple images, and murals are never simple paintings. Before the Tang Dynasty, most unknown artists were artisans, and many artists still worked on murals in temples and palaces, and independent paintings such as scrolls and sides became an important form of painting after the Tang Dynasty, changing the mechanism of painting creation. In this paper, the graphic creation process prioritizes dedication and service, but we can still feel the creativity of the painters strongly. The historical resources of how to paint these paintings, the clues to the copies, and the precursor to the foreground, encourage the painters to constantly try to resemble each other and discover problems...Therefore, in this paper, it was confirmed that reinvention and creativity are very important, and that Dunhuang Buddhist art is the basis for artists' creation and the source of vitality.

Research on Four Variables toward the Effective Integration of Culture in the EFL Class of Korea

  • Roh, Seung-Bin
    • 영어어문교육
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    • 제11권2호
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    • pp.91-110
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    • 2005
  • Many Korean EFL (English as a Foreign Language) students do not have sufficient opportunity to develop cultural knowledge and information in their classrooms. EFL teachers also tend to ignore the teaching of culture. Even though culture is taught, it simply tends to deliver "fact-only" information from the viewpoint of a "tourist level rather than cultural awareness by comparing native with target cultural references. Teaching target cultural knowledge and information should be delivered within the native cultural frame, and teaching of culture must be an integral part of teaching and learning English. The research methodology was quantitative. Quantitative data was gathered from 83 Korean EFL teachers and 286 EFL students by questionnaire. Findings indicated that three of these independent variables (cultural inequality, English-only instruction, and Unoism) were significantly and inversely related to integration of culture.

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경남지역 물리치료사의 직무만족도 및 자립개설에 대한 조사연구 (The Study about the Job Satisfaction and Independent Establishment of Physical Therapy of Working Physical Therapist in Gyeongnam Region)

  • 이준철
    • 문화기술의 융합
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    • 제4권4호
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    • pp.123-133
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    • 2018
  • 본 연구는 물리치료 인식도 개선에 필요한 기초자료를 제시하고, 자립개설에 관한 의식도를 알아보기 위하여 경남지역 물리치료사 175명을 대상으로 실시되었다. 수집된 자료는 SPSS 10.0 통계 프로그램을 이용하여 일반적 특성, 현재의 근무처 및 소속여건, 직무만족도에 대한 연구는 카이제곱검정의 빈도분석을, 자립개설에 관한 연구는 EXCEL 프로그램을 사용하였다. 통계적 유의순준 a는 0.05로 선정하였다. 성별에 따른 물리치료에 대한 흥미도는 남자가 52.2%로 여자보다 높게 나타났고, 자기능력 발전에 대해 종합병원이 32.8%로 의원보다 만족하는 것으로 나타났다. 자립개설에 대한 찬반의견은 83.4%가 찬성하였고, 자립개설 시 가장 먼저 해야 할 일은 전문적인 이론 및 임상 치료기술 습득이 57.5%로 가장 높게 나타났다. 개설 분야는 근골격계 물리치료가 45.2%, 자립개설 시 가장 걸림돌로 작용하는 것은 의사의 의식 39.7%로 나타났다. 자립개설이 이루어지기 위해서는 물리치료사들의 전문적인 이론 및 임상치료기술을 습득하고, 물리치료사와 의사간의 의식 개선이 필요할 것으로 생각된다. 또한 물리치료 관련학과를 4년제로 일원화해야 할 것이며, 물리치료사의 자립개설에 대한 법제화가 반드시 필요할 것으로 사료된다.

페미니스트 패션 브랜드의 특성과 여성 이미지 (The characteristics of feminist fashion brands and female images)

  • 임민정
    • 복식문화연구
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    • 제26권3호
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    • pp.471-484
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    • 2018
  • This study selected fashion brands claiming to advocate feminism to analyze their characteristics and female images. For the study's data, online foreign feminist fashion brands were sifted from March 2017 to January 2018 and 28 clothing brands were selected. The study's results show that feminist fashion brands aim at the demassification and individualization of fashion products to be more inclusive of individuals' physical characteristics and diversity. Additionally, feminist brands entice consumption through communication and participation in online communities and through the value of social coexistence. The essential female image produced by feminist fashion brands deconstructs a socially idealized female image and expresses a sense of self-body positivity. In turn, the concept of self-body positivity is communicated through natural images of independent women with distinct identities based on differences in race, culture, and sexual orientation. Moreover, feminist fashion brands produce social images featuring independent women using active wear to engage in social activities. Casual wear is also used to reflect active women, while mannish looks and power suits express women's social status and professional abilities. Ultimately, these offer functionally active and rational images, combined with female images featuring long hair and makeup. Yet another type of female image seeks to create a new vision of women as diverse due to their various cultures, countries of origin, races, and individual tastes. These new images express women's physical differences, distinct identities, and diversity while simultaneously deconstructing pre-existing forms of clothing.

발효식품의 마이크로바이옴 분석 기술 (Analysis techniques for fermented foods microbiome)

  • 차인태;서명지
    • 식품과학과 산업
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    • 제50권1호
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    • pp.2-10
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    • 2017
  • Human have eaten various traditional fermented foods for a numbers of million years for health benefit as well as survival. The beneficial effects of fermented foods have been resulted from complex microbial communications within the fermented foods. Therefore, the holistic approaches for individual identification and complete microbial profiling involved in their communications have been of interest to food microbiology fields. Microbiome is the ecological community of microorganisms that literally share our environments including foods as well as human body. However, due to the limitation of culture-dependent methods such as simple isolations of just culturable microorganisms, the culture-independent methods have been consistently developed, resulting in new light on the diverse non-culturable and hitherto unknown microorganisms, and even microbial communities in the fermented foods. For the culture-independent approaches, the food microbiome has been deciphered by employing various molecular analysis tools such as fluorescence in situ hybridization, quantitative PCR, and denaturing gradient gel-electrophoresis. More recently, next-generation-sequencing (NGS) platform-based microbiome analysis has been of interest, because NGS is a powerful analytical tool capable of resolving the microbiome in respect to community structures, dynamics, and activities. In this overview, the development status of analysis tools for the fermented food microbiome is covered and research trend for NGS-based food microbiome analysis is also discussed.

Duplex dPCR System for Rapid Identification of Gram-Negative Pathogens in the Blood of Patients with Bloodstream Infection: A Culture-Independent Approach

  • Shin, Juyoun;Shin, Sun;Jung, Seung-Hyun;Park, Chulmin;Cho, Sung-Yeon;Lee, Dong-Gun;Chung, Yeun-Jun
    • Journal of Microbiology and Biotechnology
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    • 제31권11호
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    • pp.1481-1489
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    • 2021
  • Early and accurate detection of pathogens is important to improve clinical outcomes of bloodstream infections (BSI), especially in the case of drug-resistant pathogens. In this study, we aimed to develop a culture-independent digital PCR (dPCR) system for multiplex detection of major sepsis-causing gram-negative pathogens and antimicrobial resistance genes using plasma DNA from BSI patients. Our duplex dPCR system successfully detected nine targets (five bacteria-specific targets and four antimicrobial resistance genes) through five reactions within 3 hours. The minimum detection limit was 50 ag of bacterial DNA, suggesting that 1 CFU/ml of bacteria in the blood can be detected. To validate the clinical applicability, cell-free DNA samples from febrile patients were tested with our system and confirmed high consistency with conventional blood culture. This system can support early identification of some drug-resistant gram-negative pathogens, which can help improving treatment outcomes of BSI.