• 제목/요약/키워드: Culture-independent

검색결과 817건 처리시간 0.03초

Identification of Lactic Acid Bacteria in Galchi- and Myeolchi-Jeotgal by 16S rRNA Gene Sequencing, MALDI-TOF Mass Spectrometry, and PCR-DGGE

  • Lee, Yoonju;Cho, Youngjae;Kim, Eiseul;Kim, Hyun-Joong;Kim, Hae-Yeong
    • Journal of Microbiology and Biotechnology
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    • 제28권7호
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    • pp.1112-1121
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    • 2018
  • Jeotgal is a Korean traditional fermented seafood with a high concentration of salt. In this study, we isolated lactic acid bacteria (LAB) from galchi (Trichiurus lepturus, hairtail) and myeolchi (Engraulis japonicas, anchovy) jeotgal on MRS agar and MRS agar containing 5% NaCl (MRS agar+5% NaCl), and identified them by using 16S rRNA gene sequencing and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) as culture-dependent methods. We also performed polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) as a culture-independent method to identify bacterial communities. Five samples of galchi-jeotgal and seven samples of myeolchi-jeotgal were collected from different regions in Korea. A total of 327 and 395 colonies were isolated from the galchi- and myeolchi-jeotgal samples, respectively. 16S rRNA gene sequencing and MALDI-TOF MS revealed that the genus Pediococcus was predominant on MRS agar, and Tetragenococcus halophilus on MRS agar+5% NaCl. PCR-DGGE revealed that T. halophilus, Tetragenococcus muriaticus, and Lactobacillus sakei were predominant in both types of jeotgal. T. halophilus was detected in all samples. Even though the same species were identified by both culture-dependent and -independent methods, many species identified by the culture-dependent methods were not in the bacterial list identified by the culture-independent methods. The distribution of bacteria in galchi-jeotgal was more diverse than in myeolchi-jeotgal. The diverse LAB in galchi- and myeolchi-jeotgals can be further studied as candidates for starter cultures to produce fermented foods.

방한 일본인 개별 자유 여행객의 한국 음식의 적응 과정 - 문화적 신념 요인들을 중심으로 - (The Adaptation Process of Korean Food for Japanese Independent Tourists (FIT) - Focus on the Cultural Belief -)

  • 최현주;한경수
    • 한국식생활문화학회지
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    • 제27권5호
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    • pp.496-504
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    • 2012
  • The objective of this study was to investigate the phasic characteristics of the adjustment process to Korean food with an analysis of Japanese independent tourist culture social resistance factors. To collect data for empirical study, a survey was distributed to 284 Japanese tourists through Korean travel agencies. Logistic regression analysis was carried out in order to determine media and cultural beliefs affecting the diffusion process of Korean food. The results showed that cultural beliefs had no significant effects on the diffusion process of most Korean food. However, people who valued similarities between the two countries-South Korea and Japan-demonstrated a significant effect on performance and identification group. Therefore, cultural beliefs did not influence the diffusion of Korean food, although there are surely cultural beliefs and subjective factors affecting their reform decisions.

도시문화와 공동체 의식의 영향 관계 (An Influential Relationship between Urban Culture and Community Spirit)

  • 김동윤
    • 한국디지털건축인테리어학회논문집
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    • 제13권4호
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    • pp.51-60
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    • 2013
  • With regard to urban culture this study aims to essential understanding and systematic approach to the culture. The "2012 Seoul Survey" report has been used to find out causality among the related variables. In the first place 'satisfaction of cultural condition' was operationally selected as a dependent variable for regression. For the purpose of controlling the third factors for ceteris paribus effect correlation analysis was previously done between the dependent variable and all other variables respectively, which resulted in two groups of variables: group (1) - 2 variables of very significant correlations(p-value<0.01) and (2) - the other 6 variables of significant correlations(p-value<0.05). Then hierarchical regression was adopted to these 2 groups to analyse statistical significance of independent variables, and multicollinearity(VIF; variance inflation factor). Additionally to OLS robust and bootstrapping regressions were done to confirm the validity of this model specification. At last a regression model specified by group (1) as independent variables(they are 'community spirit caring for women, the disabled, the poor and the old,' 'satisfaction of bicycle riding condition' shows that the variables have statistically significant and substantially strong effect on 'satisfaction of cultural condition.' This finding implies the following understanding; (1) urban festivals are regarded as the main of the urban culture as of now and this results from the low level of today's culture, (2) culture is telling and hearing stories but the influential relationship between urban culture and community spirit on the weak is negative, which says that the cultural perception among citizen is somewhat selfish and far from the essential understanding of the urban culture. In spite of restrictive external validity this finding can be used as a direction for promoting culture and a basis for related policy choice in cities.

티셔츠 원 포인트 그래픽에 나타난 Mix & Match 시각 이미지 분석 (The Analysis of Mix & Match Visual Image of One-Point Graphic Appeared in T-shirt)

  • 이미연
    • 복식문화연구
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    • 제13권5호
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    • pp.777-789
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    • 2005
  • Mix & Match Visual Image, appearing in T-shirt one-point graphics, is a new visual image emerged as a result of several trends: development of cutting-edge machinery and computer programs, various needs of consumers, and an international trend of fusion concept. Thus, a concrete research of Mix & Match Visual Images is demanding. This paper studies one-point graphic, which appeared in the SamsungDesignNet, Interfashion Planning, and firstviewkorea, with a focus on apparel T-shirt of women's wear and casual wear from 2001 to 2004. The study shows that the most preferred independent visual image in Mix & Match Visual Images is a typography, and next is in the order of marks, symbols, geometric diagrams and abstract shapes, animals and plants, characters, materials, and collages. As Mix & Match of independent visual images was mainly combined typography with other independent visual images, we need to develop more Mix & Match Visual Images using various independent visual images's mix and match. Then, T-shirt will be one of the most representative items in Korean fashion trend that enable consumers to express their characters, escaping from the old-fashioned concept that T-shirt is just a T-shirt.

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Feeder Independent Culture of Mouse Embryonic Stem Cells

  • Kim, Myoung Ok;Ryoo, Zae Young
    • Reproductive and Developmental Biology
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    • 제36권4호
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    • pp.291-294
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    • 2012
  • Embryonic stem cell classically cultured on feeder layer with FBS contained ES medium. Feeder-free mouse ES cell culture systems are essential to avoid the possible contamination of nonES cells. First we determined the difference between ES cell and MEF by Oct4 population. We demonstrate to culture and to induce differentiation on feeder free condition using a commercially available mouse ES cell lines.

간호 조직문화 유형에 따른 간호사의 기본심리욕구, 긍정심리자본, 심리적 소진 간의 관계 (Relationship between Basic Psychological Needs, Positive Psychological Capital, and Psychological Burnout depending on Types of Nursing Organizational Culture)

  • 조영문
    • 디지털융복합연구
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    • 제19권4호
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    • pp.413-421
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    • 2021
  • 본 연구는 간호조직의 조직문화 유형에 따른 기본심리욕구, 긍정심리자본, 심리적 소진의 정도와 관계를 파악하기 위한 서술적 조사연구이다. 연구대상자는 2020년 5월부터 2020년 6월까지 M시에 소재한 5개 중소병원 간호사 151명을 대상으로 하였으며 자료수집은 자기보고 설문지법을 사용하였다. 자료분석은 SPSS/WIN 23.0 Program을 이용하여 기술적 통계, one-way ANOVA, independent t-test, Pearson correlation coefficients로 분석하였다. 연구결과 관계지향문화와 혁신지향문화 유형은 기본심리욕구, 긍정심리자본과 정적 상관관계가, 심리적 소진과는 부적 상관관계가 있었다. 본 연구는 관계지향문화와 혁신지향 문화유형이 간호사의 긍정심리에 정적 관계가 있다는 것을 확인하였다. 그러므로 관계지향문화와 혁신지향문화를 활성화할 수 있는 간호조직 내 시스템 구축과 관련 프로그램 개발이 필요하다.

조직의 안전문화에 대한 인식이 개인의 직무만족, 조직몰입에 미치는 영향 - 조직신뢰의 매개효과- (The Effect of Safety Culture on Job Satisfaction and Organizational Committment - Mediation effect of Organizational Trust-)

  • 이경재;송광선
    • 대한안전경영과학회지
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    • 제15권3호
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    • pp.71-81
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    • 2013
  • The purpose of this paper is to examine the effect of safety culture on job satisfaction and organizational commitment and mediation effect of Organizational trust between independent variable and two dependent variables. Based on the responses from 290 employees, the results reveals ; 1) Organizational safety culture has a positive effect on the job satisfactions and organizational safety culture effect positively on the organizational commitment 2) Organizational trust mediates the relationships between safety culture and job satisfaction. 3) Organizational trust mediates the relationships between safety culture and organizational commitment.

물방울 무늬의 크기와 배색 변화가 원피스 드레스 이미지에 미치는 영향 - 명도 대비 배색을 중심으로 - (The Effect of Dot Pattern Size and the Variation of Coloration on Dress Wearers' Image Formation - Focused on Coloration of Value Contrast -)

  • 김선미;정수진
    • 복식문화연구
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    • 제16권5호
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    • pp.863-877
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    • 2008
  • The purpose of this study is to investigate the effect of dot pattern size(0.8, 1.8, 2.5, 5, 8), color combination (BG/R, Y/B), value tone(lt/dk, p/g), area-ratio on image information. Sets of stimulus and response scales(7 point semantic) were used as experimental materials. The stimuli were 20 color pictures manipulated with the combination of dot pattern size, color combination, value tone and area-ratio using computer simulation. The subjects were 240 female undergraduates living in Gyeongsangnam-do. Image factor of the stimulus was composed of 4 different components, visibility, chastity.feminity, cuteness and attractiveness. In the visibility, color combination, value tone, area-ratio, dot pattern size showed independent effect. In the chastity feminity, color combination, value tone, showed independent effect. In the cuteness, value tone, area-ratio, dot pattern size showed independent effect. Significant interaction effects of color and area-ratio combination on visibility and cuteness were found. Interaction efforts of color and value tone combination, value tone and area-ratio was significant on cuteness. For visibility image, BG/R combination of color and yellow background/blue dots were effective. For cuteness image, pale/grayish tone and background/dots area-ratio were effective.

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Costume color design as a symbolic expression in the independent film <Bittersweet Life> - Images in situations of movie location -

  • Kim, Jang-Hyeon;Jun, Yuh-Sun;Kim, Young-Sam
    • 복식문화연구
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    • 제22권1호
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    • pp.167-182
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    • 2014
  • This study researched the symbolic meaning of the costume colors in the independent film . The purpose of the study is to shed light on the relation between a film and the clothing color by exploring the symbolic meaning of costume colors as based on place images and as reflected in characters' internal psychological states and situations. The conclusions of this study are as follows. First, the colors primarily presented were neutral shades of black and white or dark, almost-black tones, representing the negative narrative structure and depicting the image of a human being. Second, a series of bright blue shades were presented to reflect the positive narrative structure of a human being returning successfully to reality. Third, the application of color, involving both the psychological aspect as well as the symbolism of color, was effectively delivered to the audience. Fourth, the emphasis on color in the smallest details of costumes was analyzed, and it was found that symbolic color effectively communicated the whole image of transformation as the story progressed, clearly delivered delicate emotional messages, and contained symbolism relating to particular places and situations.