• Journal of Biosystems Engineering
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• 제32권2호
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• pp.109-114
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• 2007

In mass production of seed-potato plantlets, the processes for in vitro propagation and ex vitro acclimatization with a high cost should be improved by a culture system with environmental control using scaled-up culture vessels. The experiment was conducted to design a hydroponic culture system for enhancement of growth and development of seed-potato (Solanum tuberosum) plantlets cultured under photoautotrophic (without sugar in culture medium) conditions with controlled light intensity and ventilation rate. The culture system was consisted of scaled-up culture vessels, ventilation pipes, a multi-cell tray and an environmental control system (ECS) for optimum controlling in temperature, light intensity, ventilation rate, and culture-medium supply. Growth and development of the plantlets was significantly increased under the ECS compared with a conventional culture system (CCS) of photomixotrophic culture (with sugar in culture medium) using small scale vessels. For 21 days, leaf area of the plantlets was expanded more than 2 times, and number of internodes also approximately 4 times greate. under the ECS. In addition, the photoautotrophic growth in sweetpotato (Ipomoea batatas) and chrysanthemum (Chrysanthemum morifolium) plantlets was greater more than 2 times compared with the CCS.

• 대한인간공학회지
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• 제35권2호
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• pp.111-123
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• 2016

Objective: The objective of this research is to study preceding literature on safety culture surveying tools and indicators used in aviation organizations to help the further understanding of aviation safety culture by presenting Korea-Safety Culture Survey Indicator (K-SCSI) as a relevant case. Background: The aviation field puts a great deal of effort in preventive safety management through the application of Safety Management System (SMS), which was co-developed by international aviation organizations such as ICAO and FAA. To successfully operate safety management system, safety culture factors such as the organization member's level of consciousness, attitude and faith regarding safety must be put together. However, the aviation field currently lacks programs to promote safety culture and the exact understanding of some safety culture concepts. Method: This research inquired into the definition of safety culture in the aviation field and the surveying tools used to measure it. It then described the development and application process of the Korea-Safety Culture Survey Indicator (K-SCSI) mainly focusing on case studies. Results: In this research are presented safety culture promoting programs that can be applied to subordinate indicators of K-SCSI such as organization commitment, management involvement, rationality of reward system, employee empowerment and reporting system. Conclusion: For a mature safety culture to settle successfully, it is essential that safety culture survey indicators are developed and applied in a way that fits the organization's features. Also, behavior measuring indicators are required to develop a more objective indicator and thus must be standardized. Application: Cases that deal with the development and application of safety culture measuring tools within the aviation field can be studied and applied in other domains to spread safety culture.

• 한국정밀공학회지
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• 제28권4호
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• pp.526-533
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• 2011

This research is about the pulsatile pump system utilized in the perfusion bioreactor for the in vitro human tissue culture. A pulsatile pump system which can be applied to the culture of the vascular tissues including blood vessel is developed by using the idea of human heart's blood pumping into organs as followings: culture chamber, a pressurizing device which generates laminar pulsatile flow by controlling the x-sectional area of the culture media delivering tubing, a compliance chamber which supplies the pressuring device with a constant pressure, and a peristaltic pump which circulates the culture media in a circuit ranging from the culture chamber to the compliance chamber. The developed pulsatile pump system shows that a physiology of the human heart's blood pumping including pulsatile pressure waveform of systolic-diastolic pressure is well represented. Not only time domain but also frequency domain characteristics of pulsatile pump system which are necessary for the vascular tissue culture such as pulsatile pressure waveform's shape, the frequency, and the magnitude can be easily generated and manipulated by using the proposed system.

• 한국조리학회지
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• 제8권3호
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• pp.107-122
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• 2002

The analysis result regarding the influence where the enterprise culture goes mad to a duty satisfaction with afterwards is same. First. The enterprise culture factor who causes an effect in the supervision factor of duty satisfaction joint ownership of management idea, appeared with the example by leadership factor. Second. The enterprise culture factor who causes an effect in the promotion factor of duty satisfaction appeared with the all feeling factor of system. Third. The enterprise culture factor who causes an effect in the wages factor of duty satisfaction appeared with the all feeling factor of system. Fourth. The enterprise culture factor who causes an effect in the duty form factor or duty satisfaction clearness or example by leadership and belief, duty affirmation appeared with the factor. Fifth. The enterprise culture factor who causes an effect in the service environment factor of duty satisfaction appeared with the all feeling factor of system. Conclusion the enterprise culture appeared with the fact that it causes an effect duty satisfactorily.

• 한국동물학회지
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• 제37권2호
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• pp.255-261
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• 1994

Recombinant human pBrathyriod hormone related peptide (1-341 (rhPTHrP) has been known to stimulate bone resorption in intact bone tissue culture system. Osteoclast has been known as a primary responsible cell for bone resorption. To examine the effect of rhPTHrP on this cell, we employed disaggregated rat osteodast culture. As a result, we found that rhPTHrP sisnificBntly elevates both the number and total area of resorbed pits in this culture. On the other hand, the conflicting results between disagsregated rat osteoc13st culture and Ca2+-deficient hen osteoclast culture system have been a big obstacle for the progress of bone research. To verify the differences between rat 3nd chick osteoclast system, we performed the same experiment using chick embryonic osteoclast. Since the similar results were obtained from the disaggregated chick osteoclast culture, the discrepancy between chick and rat osteoclast culture study seemed to be due to the difference in culture method, rather due to the species-difference.

• Biotechnology and Bioprocess Engineering:BBE
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• 제5권2호
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• pp.123-129
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• 2000

A high cell density culture system for the anchorage dependent CHO cells was developed based on the combination of in removal of ammonium ion and microcarrier culture system, and semi-fed-batch feeding of glucose and glutamine was employed to the developed culture system. The glass bead was selected as an optimum microcarrier in terms of cell growth. An ammonium ion selective zeolite, Phillipsite-Gismondine, was packed in a dialysis menium ion. The semi-fed-batch operation was employer to the novel culture system for the high density cell culture, and the results showed the cell growth was improved by 32% and tPA productivity by 250%.

• 한국수정란이식학회지
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• 제29권3호
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• pp.207-219
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• 2014

Three-dimensional (3D) culture system is useful technique for study of in vivo environment and it was used various experiments. This study was investigated to establish of embryo co-culture system and changes of PAs activity in 3D cultured endometrial cells of pigs. In results, growth of stromal cells into gel matrix were detected only with endometrial and myometrial cells. The most rapid growth of stromal cells were confirmed in $2.5{\times}10^5cells/ml$ and gel matrix containing 15% FBS. Expression of urokinase-PA (uPA) after treatment of hCG (0.5, 1.0, 1.5 and 2.0 IU/ml) were higher than without hCG, but, there are not significant difference among the treatment. On the other hand, expression of uPA after treatment of $IL-1{\beta}$ (0.1, 1, 10 and 100 ng/ml) were higher than without $IL-1{\beta}$, but, there are not significant difference. Expression of uPA after treatment of estrogen (0.2, 2, 20 and 200 ng/ml) were not difference, but PA activity was significantly decreased (p<0.05). Blastocyst was producing in PZM-3 medium containing FBS and endometrial cells were grown in PZM-3 medium. When embryos development with cultured endometrial cells, cleavage rates were not significant difference and blastocyst were not produced in co-culture with stromal cells and 3D culture system. 3D culture system had similar activity to in vivo tissue and these features are very useful for study of in vivo physiology. Nevertheless 3D culture system was not proper in embryo co-culture system. Therefore, we suggest that 3D culture system with embryo co-culture need continuous research.

• 한국지능시스템학회논문지
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• 제20권2호
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• pp.267-272
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• 2010

본 연구에서는 기업문화의 유형과 기업문화의 구성요소간의 관계, 영향도를 분석하고, 기업문화의 유형 평가모델에 의하여 유형을 평가하는 방법을 제시하고자 한다. 중소기업의 구성원을 대상으로 설문을 통해 구한 314개의 데이터를 사용하여 기업문화의 유형과 기업문화의 구성요소간의 관계는 상관분석을 통해, 기업문화의 유형별로 어떤 기업문화 구성요소가 영향을 주는 정도에 대해서는 회귀분석을 통해서 분석하였다. 마지막으로 기업문화 유형의 평가모델 분석은 퍼지시스템을 이용하였다. 본 연구에서의 기업문화 유형의 평가모델은 가능적인 면과 필연적인 면이 섞여 있는 형태이며, 평가모델을 이용하여 모델링에 의한 기업문화유형에 대한 추정퍼지관계행렬을 동정하여 퍼지시스템을 구하는 동정문제로서 모델의 검토를 통해서 그 유용성을 보였다.

• 한국시스템다이내믹스연구
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• 제7권1호
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• pp.173-212
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• 2006

This paper attempts to provide a more complete acknowledging of Hallyu(Korean Wave), using systems thinking. The central concept is understanding how all the objects of Hallyu(Korean Wave) in a culture system interact with one another. What it attempts to do is understand the feedback loop of Hallyu(Korean Wave) with other country culture, and thus understand the behavior of mass culture agent it make produce. This works is for public management and policy in culture industry and leading culture itself. And alternative policies that alleviate the problem, and implements this solution at Hallyu(Korean Wave) continuance.

• 한국항공운항학회지
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• 제20권2호
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• pp.64-71
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• 2012

In field of Aviation Maintenance, honest and expedite voluntary report of potential hazard provide airworthiness aircraft by eliminating or avoiding from dangerous factors of aircraft. Although it supports for safety flight, voluntary incident reporting system consist of Aviation practitioner and require cooperation of practitioner due to there are no forcibleness. These occur when positive safety culture and report culture are settled. In this regard, this study firstly identify the current status of Aviation Safety Reporting System in Korea. Then, this article also find out the level of reporting culture of the AMT(Aircraft Maintenance Technicians) and problems in reporting system. Finally, suggestions on the model of positive safety reporting culture in a field of aircraft maintenance.