• Title/Summary/Keyword: Culture system

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In Vitro Development of Nuclear Transplantation Bovine Embryos Using In Vitro Fertilized Embryos of Korean Native Heifers (한우 체외수정란을 이용한 핵 이식배의 체외발달에 관한 연구)

  • 박충생;공일근;노규진;이효종;최상용
    • Korean Journal of Animal Reproduction
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    • v.18 no.2
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    • pp.113-119
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    • 1994
  • To improve nuclear transplantation(NT) efficiency and to produce a large scale genetically identical cloned calves, examined the in vitro development capacity after co-culture of bovine oviductal epithelial cells (BOEC) and granulosa cells in TCM-199 supplemented with 10% fetal calf serum (FCS) with early bovine embryos derived from in vitro matured fertilized(IVM-IVF) oocyte. In addition, the age dependence of IVM oocyte on electro-stimulation and the effective electric voltage on in ivtro development of bovine NT embryos were examined. The results obtained were summerized as follows; 1. The cleavage rates of IVM-IVF bovine embryos in co-culture with bovine oviductal epithelial cells and granulosa cells were not significantly different(P<0.05), but the developmental rate into morula and blastocyst stage were different showing 38.3 and 20.2%, respectively. 2. The activation (82.5%) and development in vitro(8.6%) into later embryo stages of the aging oocytes of 32 hours post-maturation (hpm) were significantly higher than those of 24 hpm at direct current (DC) voltage of 1.5kV/cm, 60$\mu$sec pulse duration and 1 pulse time. 3. The fusion rates of NT eggs of 32 hpm following to different DC voltages from range 0.75 to 1.5kV/cm were not differ, but the developmental rate into morula and blastocyst stages at DC voltages of 0.75 and 1.0kV/cm were higher(11.4 and 12.6%, respectively) than those of 1.5kV/cm(0%). From these results, it can be suggested the optimal culture system for in vitro culture of IVM-IVF bovine embryos is a co-culture system with BOEC in TCM-199 supplemented 10% FCS. The effective time and the DC voltage for activation, electrofusion and in vitro development of NT embryos derived from IVM-IVF bovine embryo are 32hpm and 0.75~1.0kV/cm. But to improve NT efficiency, the advanced research (cell cycle synchronization, micromanipulation, culture system, etc.) is needed.

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An Investigative Stucy on the Farmland Uses in Korea and Hokkaido of Japan (韓國과 日本 北海道에서의 農地利用에 관한 調査硏究)

  • Kim, Ki-Sung;Yasuharu, Uneda;Choi, Ye-Hwan
    • Magazine of the Korean Society of Agricultural Engineers
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    • v.33 no.3
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    • pp.81-90
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    • 1991
  • The purpose of the this study was to investigate and analyze the various statistical data on the actual state of farmland uses given in Korea and Hokkaido of Japan. The results of this research are as follows ; 1. The rate of farmland uses was marked 118% and 99% in Korea and Hokkaido of Japan in 1988, respectively. That of Korea was higher than that of Hokkaido of Japan. The tendency of the value for Korea has been decreased since the value was shown peak in 1980, but that of the value for Hokkaido was found gradually the increased value. It was analyzed that the reason why the value was decreased in Korea was referred to avoid the crops culture, and the reason why the value was increased in Hokkaido was dueto increase the area of pasture. 2. The farmland use system according to region were d ue to be the mixing pattern of rice culture and dry-field crops in East region of korea and the type of mainly rice culture in West region. The pattern of farmland use system was used to be performed mainly the rice culture in Central region, the mixing type of rice culture and dry-field crops in South region, and the agriculture of mainly dry-field crops and dairy in East-North region in Hokkaido. 3. In the light of the rate of planting, the rate of rice culture and dry-field crops were 49% and 51% in Korea, respectively. As the values were 13% and 87% in Hokkaido of Japan, respectively, the dry-field crops attained the superiority. The main crop kinds was shown the rice culture in Korea and the feed plant in Hokkaido of Japan, respectively. 4. In considering the above results, there are much differences in agricultural style and industrialization processes in both country Korea and Japan. Especially much more differences were evident from farmland use system of Hokkaido Island where had unique agricultural style in Japan.

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An Research Into The Reactive Safety Action Program for Promoting Aviation Safety Culture

  • Kim, Dae Ho
    • Journal of the Ergonomics Society of Korea
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    • v.35 no.3
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    • pp.165-173
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    • 2016
  • Objective: The objective of this research is to inquire about safety information from the standpoint of its usefulness to suggest the significance of the Reactive Safety Action Program, which serves to promote aviation safety culture. Background: Safety information plays an important role in operating safety programs. Each organization learns lessons from safety information collected from aviation accidents and incidents. When an accident occurs, it is only through safety investigation and a close inquiry on the cause that we can come up with an appropriate countermeasure which would contribute to preventing the recurrence of the same or similar accident. However, the usefulness of safety information produced from unsatisfactory safety investigation is insufficient. Method: This research analyzed the characteristics of aviation accidents, the differences between safety investigations and legal accident investigations in systematic and operative perspectives, and safety culture as a measure to activate reporting systems (compulsory/voluntary). Results: This research defined the investigation scope and processes of safety investigations and legal accident investigations. It also suggested factors such as just culture based on trust, non-punitiveness, confidentiality, the participation of the entire staff through the use of inclusive reporting base, ensuring the independence of the operating organization as a way to promote safety through reporting systems. Conclusion: The organization's effort is the important aspect in obtaining exact and accurate safety information from accidents/incidents. The separate running of SIB (Safety Investigation Board) and AIB (Accident Investigation Board), the systematization of safety information reporting system, and prescribing (legislating) the composition of related organizations are some representative programs. Application: This research inquired experiences that contributed in promoting aviation safety culture in a reactive perspective, and will serve a role in spreading safety culture by enabling the use of application experiences of the aviation field in other domains.

A Study on the Analysis and Improving Plan of the Local Culture & Tourism Website : Focusing on the Culture & Tourism Information System of Busan Metropolitan City (지역문화관광 웹사이트의 분석 및 개선 방안에 관한 연구 : 부산광역시의 문화관광정보시스템을 중심으로)

  • Kim, Hee-Kyung
    • Journal of Information Management
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    • v.38 no.4
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    • pp.88-118
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    • 2007
  • The purpose of this research is to analyze the problems of regional culture and tourism websites and to seek for the ways to improve those websites since the culture and tourism information websites of local government do not provide enough contents and service. This research is based on following 4 indicators, content evaluation, usability evaluation, design evaluation and interactivity evaluation which are used to evaluate tourism homepages of municipality by Korea Tourism Organization. The result of this research came out with the issue that regional cultural tourism websites lack user-oriented contents. Therefore, regional culture and tourism websites need to provide the custom-made information such as setting a free tourist route, complementing reservation system and diversifying the range of search.

Contribution of the Free Learning Semester Programs of Public Library to Local Development: Focused on Cases of Busan City (공공도서관 자유학기제 프로그램의 지역발전 기여 - 부산지역 사례를 중심으로 -)

  • Yoon, Hee-Yoon;Kim, Gyoung Hee
    • Journal of the Korean Society for Library and Information Science
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    • v.53 no.2
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    • pp.29-48
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    • 2019
  • The free learning semester system focuses on the activation of career education of middle school students as an educational policy that links career recognition in elementary school, career search in middle school, and career planning in high school. This system was fully implemented in 2016 and public libraries also provided various programs. This study analyzed the free learning semester system programs of public libraries in Busan city and demonstrated the contribution of local development. As a result, career and job search, career exploration and experience, and information literacy enhancement programs contributed to local knowledge culture, reading culture, learning culture, living culture and leisure culture. However, contribution of reading exhibitions, job experience, information literacy enhancement to the leisure culture and local economy were limited. Therefore, it is desirable that all libraries should add programs related to knowledge ecosystem structure, digital information gap, human healing, social environment issues, future job prospects, and provide information literacy programs.

The Study on Classification System of Sport Culture Contents for Korea's Cultural Competitiveness (문화경쟁력 제고를 위한 스포츠 문화콘텐츠 분류체계 정립)

  • Lee, Soo-Yeon
    • 한국체육학회지인문사회과학편
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    • v.55 no.2
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    • pp.111-121
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    • 2016
  • The purpose of this study was to establish a classification systems of sport culture contents for enhancing cultural competitiveness. The main research method for this was the Delphi survey based on the literature. Through a literature review, the definition of sport cultural contents and the classification system of major industrial countries were analyzed. The Delphi survey by a panel of 25 was conducted over a two-round. The results were as follows: first, the sport culture contents includes an artistic creativity, which have a working form that enables delivery to the public and organizing sports activities, and classify as a technology for high value-added content and services. Second, the 'culture' for the large category item, which include the cultural heritage and culture art. The 'media' recognize the importance of temporal concepts with traditional media and it was classified as new media. In the case of items in the large category 'events' are classified as national and international sporting events and festivals, the 'services' main category item is divided into social, care, educational services. Finally, the 'others' includes such as copyrighted items.

A Study on the Corporate Culture and the Organizational Effectiveness (기업문화와 조직유효성에 관한 연구)

  • 김재붕;양시영
    • Journal of Korean Society of Industrial and Systems Engineering
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    • v.20 no.44
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    • pp.425-446
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    • 1997
  • In recent years, a scholars and a managers in business have taken a more interest on the effects of corporate culture on the organization effectiveness. The corporate culture has been recognized as one of the way to promote the organization performance and cope with the changes of the business environment. Edgar H. Shein defins the corporate culture as the that the pattern of basic assumptions that a given group has invented, discovered or developed in learning to cope with its problems of external adaptation and internal integration. The organization effectiveness is differently defined as the job satisfaction, job involvement, organizational commitment, organizational performance etc. The business culture help the employee (1) understand the values, the tradition, and the different management systems(decision-making basis, promotion, reward, behavior etc.) of the company, (2) make the performance-oriented decison and doings. Consequently, the excellent corporate culture would improve the organizational effectiveness. The purpose of this study is (1) to examine theoretically the content on the corporate culture and the organizational effectiveness, (2) to suggest the direction of the corporate culture management for both the management md the scholars.

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Usability of Digital Encyclopedia of Korean Local Culture(www.grandculture.net) for local identity (지역정체성 형성을 위한 향토문화전자대전(www.grandculture.net)의 활용)

  • Yun, Yu-Seok
    • 한국HCI학회:학술대회논문집
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    • 2008.02b
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    • pp.546-551
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    • 2008
  • "Encyclopedia of Korea Local Culture(www.grandculture.net)" is a digital encyclopedia system for local culture. It contains a local history, culture geography and who is who HCI wants computer to help a human life. Then what is useful information for human and human culture? Maybe a knowing of own local culture makes one more happy. because it will help to understand oneself This article will show how this encyclopedia manages the cultural resources of local.

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A Study on the Effect of Safety culture on safety in railway industry (철도산업에서 Safety culture 가 Safety에 미치는 영향에 관한 연구)

  • Heo, Eun-Mee;Kim, Sa-Kil;Byun, Seong-Nam
    • Proceedings of the KSR Conference
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    • 2007.11a
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    • pp.1783-1786
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    • 2007
  • The purpose of this study is to analyze human safety culture of the railway driver in domestic railway industry. This study proposes safety culture improvement of domestic railway by intensively comparing railway safety system overseas to reinforce domestic railway safety. Domestic railways well known the well-organized railway safety culture has brought up safety issues on their process of private management. With this process, also, domestic safety culture is requested by increasing sense of the safety for general public. This study expects Korean railways to become a well-organized that as removing insecure factors of domestic railway based on result comparing and analyzing the manual, factors and requirements of guideline for internal and external safety culture.

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Analysis of Operational Effect of Public Culture & Arts Institution (공공 문화예술기관의 운영효과 분석)

  • Kim, Shin-Pyo;Cho, Hyun-Joo
    • Journal of Industrial Convergence
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    • v.14 no.2
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    • pp.39-46
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    • 2016
  • The purpose of this Study is to present the tasks and means of improving the operational system of public culture and arts institutes in the future on the basis of the analysis of the effectiveness of operation of public culture and arts institutes. The summary of the results of analysis in this Study is as follows: Firstly, it was found that the factor variables that impart effect on the operation of public culture and arts institutes included (1) artistic value, (2) convenience and (3) organizational management, while the performance variable of public culture and arts institutes was (4) operational performance. Secondly, in the analysis of the structure that explains the operational effects of public culture and arts institutes, convenience and organizational management were found to impart effects on the operational performances. However, artistic value was found to have no effect on operational performances. Therefore, these results of analysis imply that organizational management and convenience, rather than artistic value, need to be fortified in order to elevate the operational performances of public culture and arts institutes.

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