• Journal of Plant Biotechnology
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• v.6 no.4
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• pp.229-233
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• 2004

Partial desiccation of embryogenic calli cultures or somatic embryos leads to different physiological changes and maturation of somatic embryos, leading to improved plant regeneration. Embryogenic calli was induced from immature inflorescence segments and young leaf rolls of sugarcane (Saccharum officinarum hybrids CoC-671) on Murashige and Skoog's basal medium enriched with different concentrations of 2,4-D ($1-4\;\cal{mg/l}$), L-glutamine ($100\cal{mg/l}$), malt extract ($100\cal{mg/l}$), casein hydrolysate ($1000\;\cal{mg/l}$) and coconut milk ($5\%$) and solidified with $0.2\%$ gel rite. The embryogenic calli were subjected to desiccation for 1-8 h. Desiccation of the calli for 6-7 h resulted in enhancement of plant regeneration frequency ($83-96\%$) as compared to control ($12\%$). Plantlets exhibited vigorous growth to maturity in the greenhouse. Partial desiccation of embryogenic calli offers as a simple method for improving plant regeneration frequency in sugarcane.

• Plant Biotechnology Reports
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• v.3 no.4
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• pp.341-345
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• 2009

This study describes culture conditions for a plant regeneration system via a combined pathway of somatic embryogenesis and organogenesis in root explant cultures of the commercial rose cultivar 'Charming'. Root explants formed white calluses at a frequency of 30% after 6 weeks of culture on Schenk and Hildebrandt (SH) medium supplemented with $11mg\;1^{-1}$ 2,4-dichlorophenoxyacetic acid. After 6 weeks of transfer to SH medium without growth regulators, initial white calluses gave rise to globular somatic embryos at a frequency of 2.8%, which were subsequently dedifferentiated to embryonic tissues. Somatic embryos or embryonic tissues initially derived from root explants did not undergo development beyond cotyledonary stage. To produce adventitious shoots, embryonic tissues were sliced and cultured on SH medium with $0.5mg\;1^{-1}$ 6-benzyladenine. After 4 weeks of culture, 28% of embryonic tissue explants formed adventitious shoots. Regenerated shoots were rooted on half strength SH medium with $0.1mg\;1^{-1}$ ${\alpha}-naphthalaneacetic$ acid and subsequently grown to maturity. Root-derived embryonic tissues were proliferated by subculture, while retaining the capacity for shoot production for a few years.

• Animal cells and systems
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• v.4 no.4
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• pp.329-336
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• 2000

Life table experiments were performed in order to examine the effects of food quality on Moina macrocopa fed with four kinds of algal foods, Botryococcus sp., Scenedesmus subspicatus, Selenastrum capricornutum, and Chlorella sp., at $20^{\circ}C$ . The temperature effects on M. macrocopa were also assessed, feeding Chlorella at $17^{\circ}C$,$20^{\circ}C$,$25^{\circ}C$, and a combination of $28^{\circ}C$ (light) and $25^{\circ}C$ (dark). The cartilaginous Botryococcs cells were Inappropriate food for Moina. Among the foods tested, Chlorella was the food of the best quality in all accounts of life history traits. Moina grown on Chlorella showed higher net reproductive rate ($R_0$), longer mean and maximum longevities, earlier mean age at maturity, longer mean carapace length at maturity, larger mean clutch size, and shorter mean time interval between clutch productions than those grown on Selenastrum and Scenedesmus. An optimal temperature for Moina was $20^{\circ}C$ . When Moina were grown on Chlorella at $20^{\circ}C$, they showed the highest r, the highest $R_0$, the shortest T, the longest mean longevity, the earliest mean age at maturity, the longest mean carapace length at maturity, and the largest mean clutch size. The results of life table experiments showed that the individual and population growth patterns were much more affected by low temperature $17^{\circ}C$, than by high temperature ($\geq 20^{\circ}C$). In the optimal condition, the r value was very high, 5.1 in $d^{-1}$. In conclusion, the food quality and the temperature are the most important factors to govern the size and continuity of Moina population, by which the individual growth rates and reproductivity of members in the population can be controlled to survive in their environment such as small and temporary water bodies in nature.

• Clinical and Experimental Reproductive Medicine
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• v.44 no.2
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• pp.85-89
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• 2017

Objective: The aim of this study was to explore the effects of the insemination method on the outcomes of elective blastocyst culture. Methods: We retrospectively analyzed the outcomes of elective blastocyst culture performed between January 2011 and December 2014. Results: There were 2,003 cycles of conventional in vitro fertilization (IVF) and 336 cycles of intracytoplasmic sperm injection (ICSI), including 25,652 and 4,164 embryos that underwent sequential blastocyst culture, respectively. No significant differences were found in the female patients' age, basal follicle-stimulating hormone level, basal luteinizing hormone level, body mass index, number of oocytes, maturity rate, fertilization rate, or good-quality embryo rate. However, the blastocyst formation rate and embryo utilization rate were significantly higher in the conventional IVF group than in the ICSI group (54.70% vs. 50.94% and 51.09% vs. 47.65%, respectively, p<0.05). The implantation/pregnancy rate (IVF, 50.93%; ICSI, 55.10%), miscarriage rate (IVF, 12.57%; ICSI, 16.29%), and live birth rate (IVF, 42.12%; ICSI, 44.08%) were similar (p>0.05). No cycles were canceled due to the formation of no usable blastocysts. Conclusion: Although the fertilization method had no effect on clinical outcomes, the blastocyst formation rate and embryo utilization rate in the ICSI group were significantly lower than those observed in the conventional IVF group. Therefore, more care should be taken when choosing to perform blastocyst culture in ICSI patients.

• International Journal of Advanced Culture Technology
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• v.8 no.2
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• pp.12-17
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• 2020

This study was a similar experimental study that analyzed the effect by applying the learning portfolio completion. The study period lasted from October 1, 2019 to November 20, 2019. A total of 47 people participated in the study, and the effectiveness of the program was analyzed with the SPSS 18.0 program for critical thinking disposition. The statistical analysis method was frequency analysis and paired t-test. As a result of the analysis, the critical thinking disposition increased significantly in the application of the learning portfolio completion (Truth-seeking MD= -0.05, p <0.01), Open-mindness MD= 0.11, p <0.001), Analyticity MD= 0.76, p <0.001), Systematicity MD= -.25, p <0.001), Self-confidence MD=-0.54, p <0.001), Inquisitiveness MD=0.29, p <0.001), Maturity MD=-.0.33, p <0.001). In conclusion, the teaching method applied with the learning portfolio completion actually helped nursing students learn nursing students learn based on critical thinking. Based on these result, further research using learning portfolio is to be done and more systematic and practical application of learning portfolio completion to nursing students. This study would be used as a basic data for the study guideline development for learners.

• International Journal of Human Ecology
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• v.11 no.1
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• pp.1-10
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• 2010

Two elements of paisley textile design (color and size of motif) were manipulated to investigate their effects on people's perception. Korean and Caucasian American women were selected to represent Asian and Western countries to compare the differences in image perceptions of paisley patterns between two cultures. The participants were 168 female university students composed of 84 Caucasian Americans and 84 Koreans. The experimental design was a $2{\times}2{\times}7$ factorial design: two levels of perceiver's culture, two levels of motif size, and seven levels of the motif color. The four factors used to account for image perception were an elegance factor, individuality factor, maturity factor, and femininity factor. The results of the present study confirm that image perception can be different according to the color and size of a motif and the perceiver's culture. In the results, Americans perceived the paisley pattern as more preferable than Koreans did. Red background + Orange motif was perceived as the most feminine and Dark blue background + Sky blue motif and Dark gray background + Gray motif was perceived as the most masculine in both cultures. Compared to the big motif, the small motif was perceived as more elegant in both cultures.

• The Korean Journal of Food And Nutrition
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• v.30 no.5
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• pp.935-942
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• 2017

In the present study, kohlrabi-kimchi was prepared for assessment of changes in sensory qualities and measurement of changes in quality characteristics according to the storage period for the purpose of using kohlrabi as an ingredient of Kimchi. Examination of the physicochemical properties of kohlrabi-kimchi according to the storage period showed that the acidity increased steadily throughout the storage period, while soluble-solids content, pH and hardness decreased as the storage period elapsed. Meanwhile, the L value increased and then it decreased as the storage period elapsed, but, the a value increased and the b value gradually decreased after day 14 of storage. Reducing sugars increased up to day 10 of storage, and then they decreased slowly after day 15 of storage. Measurement of changes in lactic acid bacteria in kohlrabi-kimchi according to the storage period showed bacterial growth showing typical tendencies of Kimchi with a rapid increase on day 5 of storage and a decrease thereafter. Assessment of sensory qualities of kohlrabi-kimchi according to the storage period showed that the highest scores for appearance and sourness, odor, texture, and overall scores were obtained on day 15 of storage. Based on the above results, it was determined that the optimal maturity period of kohlrabi-kimchi is day 15 of storage.

• The Research Journal of the Costume Culture
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• v.29 no.4
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• pp.488-504
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• 2021

This article examined the historical evolution of changes in the democratization of fashion, identified its characteristics, and defined 'fashionocracy - fashion democracy'- by analyzing various phenomena in the global fashion industry. This research will expand the field of fashion research and spark academic debates about fashion democracy. The democratization of fashion can be summarized in five periods; birth, introduction, early growth, growth, and maturity. The characteristics of the democratization of fashion include individual autonomy, accessibility that many people can access and enjoy, and diversity. According to the principles of democracy- "of the people, for the people, by the people" - which are based on freedom and equality, we have achieved fashion of the people and for the people so far. Furthermore, social media has shifted the balance of power to influencers and bloggers; as such, the masses who have consumed and enjoyed fashion democratization are becoming producers and promoters by actively participating in the process of making fashion, creating a new era of fashion democracy (fashionocracy): - by the people. Ultimately, fashionocracy consists of the '6P's' ; people (active and productive consumers), planet (society and environmental sustainability), products (genderless, ageless, inclusive), price (reasonable), place (multi-channel distribution, virtual spaces), and promotion (horizontal).

• Korean Journal of Organic Agriculture
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• v.23 no.3
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• pp.523-534
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• 2015

Twenty four Holstein steers (average body weight $714{\pm}13.60kg$) were used in this experiment to determine the effect of supplementing of microbial culture and coated vitamin-C on growth performances and carcass characteristics in finishing Holstein steers. Holstein steers were randomly assigned to feeding groups of control group (Con, 12 kg of basal diet/head/day), microbial culture group (MC, 12 kg of basal diet + 30 g of microbial culture/head/day) and coated vitamin-C group (CVC, 12 kg of basal diet + 10 g of coated vitamin-C/head/day). MC and CVC groups were higher in ADG compared to control (P<0.05). FCR was also lower in MC and CVC groups than control group (P<0.05). Back fat thickness, rib-eye area, marbling score, meat color and yield index were not changed by supplementing microbial culture and coated vitamin-C. MC group was higher for maturity compared to control and CVC group (P<0.05). CVC group was higher for fat color compared to control and MC group (P<0.05). Based on the results obtained from the current study, supplementation of microbial culture and coated vitamin-C as an alternative to antibiotic might increase growth performances and enhance carcass characteristics in finishing Holstein steers. However, more studies are needed to find out the optimum supplementing period of microbial culture or coated vitamin-C for high quality meat production from Holstein steers.

• Journal of Plant Biotechnology
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• v.34 no.3
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• pp.197-200
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• 2007

Mature seeds of Artemisia annua L. were placed onto Murashige and Skoog's (MS) medium supplemented with $4.52\;{\mu}M$ 2,4-dichlorophenoxyacetic acid (2,4-D). After 6 weeks of culture, off-white, compact calluses were formed on the plumule of seedlings at a frequency of 5.9%. Calluses were subcultured on the same medium. After an additional 2 weeks of subculture, calluses produced a few somatic embryos at a frequency of 28.8%. Upon transfer to MS basal medium, calluses producing a few somatic embryos gave rise to numerous somatic embryos, which subsequently developed into plantlets. Plantlets were successfully transplanted to potting soil and grown to maturity in a greenhouse.