Partial Desiccation of Embryogenic Calli Improves Plant Regeneration in Sugarcane (Saccharum Spp.)

  • Desai Neetin Shivajirao (Plant Cell Culture Technology Section, Nuclear Agriculture and Biotechnology Division, Bhabha Atomic Research Center) ;
  • Suprasanna Penna (Plant Cell Culture Technology Section, Nuclear Agriculture and Biotechnology Division, Bhabha Atomic Research Center) ;
  • Bapat Viswas Ananat (Plant Cell Culture Technology Section, Nuclear Agriculture and Biotechnology Division, Bhabha Atomic Research Center)
  • Published : 2004.12.01


Partial desiccation of embryogenic calli cultures or somatic embryos leads to different physiological changes and maturation of somatic embryos, leading to improved plant regeneration. Embryogenic calli was induced from immature inflorescence segments and young leaf rolls of sugarcane (Saccharum officinarum hybrids CoC-671) on Murashige and Skoog's basal medium enriched with different concentrations of 2,4-D ($1-4\;\cal{mg/l}$), L-glutamine ($100\cal{mg/l}$), malt extract ($100\cal{mg/l}$), casein hydrolysate ($1000\;\cal{mg/l}$) and coconut milk ($5\%$) and solidified with $0.2\%$ gel rite. The embryogenic calli were subjected to desiccation for 1-8 h. Desiccation of the calli for 6-7 h resulted in enhancement of plant regeneration frequency ($83-96\%$) as compared to control ($12\%$). Plantlets exhibited vigorous growth to maturity in the greenhouse. Partial desiccation of embryogenic calli offers as a simple method for improving plant regeneration frequency in sugarcane.


  1. Arencibia AD, Carmona E, Cornide MT, Menendez E, Molina P (2000) Transgenic Sugarcane (Saccharum species). In Bajaj YPS (eds), Biotechnology in Agriculture and Forestry 46. Transgenic Crops 1, pp 188-206. Springer Verlag, Heidelberg
  2. Attree SM, Tautorus TE, Dunstan DI, Fowke LC (1990) Somatic embryo maturation germination and soil establishment of plants of black and white spruce. Can J. Bot. 68: 2583-2589
  3. Bapat VA, Rao PS (1996) Maturation and desiccation of somatic embryos. In Pareek LK and Swarnkar PL (eds) Trends in Plant Tissue Culture and Biotechnology, pp. 53-64. Agro Botanical PUblishers, India
  4. Bower R, Birch RG (1992) Transgenic sugarcane via microprojectile bombardment. The Plant Journal 2: 409-416
  5. Capuana M, Debergh PC (1997) Improvement of the maturation and germination of horse chess nut somatic embryos. Plant Cell Tiss Org Cult 48 23-29
  6. Carman JG (1988) Improved somatic embryogenesis in wheat by partial simulation of the in ovule oxygen, growth regulator and desiccation environments. Planta 175: 417-424
  7. Changalrayan K and Meagher MG (2001) Effect of various growth regulators on regeneration of sugarcane, In vitro Cell Develop Bioi 37: 434-439
  8. Chen WH, Davey MR, Power JB, Cocking EC (1988) Control and maintenance of plant regeneration in sugarcane calli cultures. J Exp Bot 39: 251-261
  9. Etienne H, Montoro P, Ferriere M, Carron MP (1993) Effects of desiccation, medium osmolarity and Abscisic acid on the maturation of Hevea brasiliensis somatic embryos. J Exp Bot 267: 1613-1619
  10. Fki L, Masmoudi R, Drira N, Rival A (2003) An optimized protocol for plant regeneration from embryogenic suspension cultures of date palm, Phoenix dactylifera L., cv. Deglet Nour. Plant Cell Rep. 21 (6): 517-524
  11. Gallo-Meagher M, English RG, Abouzid A (2000) Thidiazuron stimulates shoot regeneration of sugarcane embryogenic calli. In vitro Cell Dev Bioi Plant 36: 37-40
  12. Gray DJ (1989) Effect of dehydration and exogenous growth regulators on dormancy, quiescence and germination of grape somatic embryos. In Vitro Cell Dev Bioi 25: 1173-1178
  13. Guiderdoni E, Merot B, Klsomtramage PF, Feldman P, Glaszman JC (1995) Somatic embryogenesis in sugarcane. In Bajaj YPS (eds) Biotechnology in Agriculture and Forestry, pp 92-113. Springer Verlag, Berlin
  14. Hammatt N, Davey MR (1987) Somatic embryogenesis and plant regeneration from cultured zygotic embryos of soybean. J Plant Physiol 128: 219-226
  15. Ho WJ, Vasil IK (1983) Somatic embryogenesis in Sugarcane (Saccharum officinarum L.): Growth and plant regeneration from embryogenic cell suspension cultures. Ann Bot 51: 719-726
  16. lidia Y, Watabe K, Kamada H, Harada H (1992) Effects of abscisic acid on the induction of desiccation tolerance in carrot somatic embryos. J Plant Physiol 140: 356-360
  17. Kermode AR, Bewley JD (1985) The role of maturation drying in the transition from seed development to maturation. Jour Exp Bot 36:1906-1915
  18. Liu MC (1993) Factors affecting induction, somatic embryogenesis and plant regeneration of calli from cultured immature inflorescence of sugarcane. J Plant Physiol 141: 714-720
  19. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15: 473-497
  20. Quatrano RS (1987) The role of hormones during seed development. In Davies PJ (eds) Plant hormones and their role in plant growth and development, pp. 494-514. Dordrecht: Martinus Nijhoff, The Netherlands
  21. Rance 1M, Tian W, Mathews H, de Kochko A, Beachy RN, Fauquet C (1994) Partial desiccation of mature embryo derived calli, a simple treatment that dramatically enhances the regeneration ability of indica rice. Plant Cell Rep 12:647-651
  22. Roberts DR (1991) Abscisic acid and mannitol promote early development, maturation and storage protein accumulation in somatic embryos of interior spruce. Physiol Plant 83: 247-254
  23. Srinivas L, Morphogenetic studies in vitro in banana. M.Sc. Thesis, Mumbai University, Mumbai, India, 2002
  24. Vasil V, Vasil IK (1985) Histology of somatic embryogenesis in cultured immature embryos of maize (Zea mays L.). Proto plasma 127: 1-8