• Title/Summary/Keyword: Culture efficiency

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Development of Cell Lines for Application of Recombinant DNA Techniques in Crops (작물의 유전자 재조합을 위한 세포주의 개발 연구)

  • Chae, Young-Am;Choi, Kyu-Whan
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.30 no.2
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    • pp.195-200
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    • 1985
  • This experiment was carried out to know the processes of protoplast isolation, culture and plant regeneration in aims of introducing foreign genes into plant cells through plant gene vector, and cellular selection for plant improvement. The main results indicated that 2% cellulase plus 0.5% macerozyme is proper for isolation of protoplasts from leaf mesophyll cells of N. plumbaginifolia, plating efficiency was higher in 1.4-2.0 x 10$^4$ cells/ml, complete cell wall was regenerated after 2 days culture, cell division and cell mass were observed after 4 days and 2 weeks, respectively, colony was developed after 3 weeks culture, addition of 1-2mg/l BA promoted shoot differentiation while root differentiation did not required hormone and seeds were harvested from more than 100 cell lines for further investigation and study.

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Basic Study for Developing Media Competency Model (미디어역량 모델개발을 위한 기초 연구)

  • Lee, Chan;Park, Yo-Song;Yi, Byung-Jun;Choi, Young-Jun
    • Korean Journal of Culture and Arts Education Studies
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    • v.6 no.3
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    • pp.21-37
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    • 2011
  • The purpose of this study was to discuss the cultural competency model which can be applied for all the fields in one's life and to develop the cultural competency of media part for school education, lifelong education, and corporate education. To achieve this purpose, the cultural competency model of media part was developed through literature review, panel workshop with high-performers of media part, and experts verification. In the result of this study, core performance of media part was identified. They were improvement of work efficiency, possibility of creative design, change of media context, increase of understanding and participation to social culture, improvement of relationship skill, increase of chance of self-examination, and self-satisfaction. It was 62 that The cultural competencies which were needed to achieve these core performance, then, finally the definition and standard of behaviors of each competencies were drawn and the cultural competency model was proposed.

Topic-oriented Liberal English Class Plan for Foreign Learners at University (대학생 외국인 학습자를 위한 주제 중심의 교양 영어 수업방안)

  • Kim Hye-Jeong
    • The Journal of the Convergence on Culture Technology
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    • v.9 no.5
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    • pp.111-117
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    • 2023
  • The aim of this study is to present a practical teaching plan for liberal arts English classes that target foreign students. Foreign learners who do not have Korean language proficiency at the university level may struggle to understand the contents of liberal arts classes conducted by Korean language professors. In this study, six topics were selected (K-culture, Online game, Harry Potter, Disney, Marvel, DC) and topic-centered participatory class activities using various media were developed. A questionnaire was conducted to analyze learners' attitudes toward and perceptions regarding topic-oriented classes. It showed that learners' satisfaction with topic-based classes was high (75%), and the reasons for this high level of satisfaction were the instructors' caring attitudes, the comfortable class atmosphere, and the fun learners had in class. Learners also reported high satisfaction with various participatory class activities (81.9%), citing the learning benefits, their increased interest and motivation, and the efficiency of participatory classes. As globalization continues to increase the number of foreign students in South Korea, the need to develop realistic class plans and various class activities that are suitable for them is becoming more and more urgent.

Enhancement of Cultivation Efficiency of Bifidobacterium longum Using Calcium Carbonate Buffer System (Calcium Carbonate Buffer System을 이용한 Bifidobacterium longum의 배양 효율 증대에 관한 연구)

  • Lee, Ki-Yong;Hwang, In-Bum;Heo, Tae-Ryeon
    • Korean Journal of Food Science and Technology
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    • v.29 no.1
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    • pp.126-132
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    • 1997
  • Calcium carbonate ($CaCO_3$) immobilized with alginate was studied as buffer system to enhance the cultivation efficiency of Bifidobacterium longum (ATCC 15707) which is inhibited at low pH. To test the bufferring effect of the immobilized $CaCO_3$ beads, pH value in each modified trypticase-proteose peptone-yeast (TPY) broth which is adjusted to pH 4.0 with acetic acid, lactic acid and complex solution of acetic and lactic acid, 3:2 (M:M) was tested by concentration of $CaCO_3$ bead and reaction time. The bufferring effect of $CaCO_3$ bead became higher with increasing the amount of $CaCO_3$ bead in the acidic solution. The growth rate of bifidobacteria and bufferring effect were examined in relation to the amount of $CaCO_3$ bead and concentration of glucose in the modified TPY media. The growth rate of bifidobacteria and bufferring effect were increased with increasing the amount of $CaCO_3$ bead and concentration of glucose. Also, the exponential time of bifidobacteria became longer with increasing the amount of $CaCO_3$ bead and concentration of glucose in the modified TPY media. When we observed the growth rate of bifidobacteria by the method of pH-controlled culture and $CaCO_3$ buffer system, the $CaCO_3$ buffer system was more effective than that of pH-controlled culture. Therefore, this $CaCO_3$ buffer system may be useful as a method to enhance of the cultivation efficiency of bifidobacteria.

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Prospects of Stable Production Technologies for Food Crops (식량 안정생산기술의 전망)

  • Chae Je Cheon;Gang Yang Sun;Lee Yeong Ho;Nam Jung Hyeon
    • Proceedings of the Korean Society of Crop Science Conference
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    • 1999.11a
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    • pp.102-144
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    • 1999
  • The major problems of food crop cultivation in Korea are low yield of most crops except rice. inefficient cultivation techniques for aged farmers. and low international competibility. Therefore, development of cultivation techniques of food crops should aim the yield. quality improvement, labor reduction and production cost. The primary issue for increasing the yield of soybean, barely and wheat is to reduce the yield gap between the farmer's yield and recommended ones of experiment station. More advanced cultivation techniques needs to be developed. and/or the conventional breeding methods to be reconsidered. The newly developed labor-saving mechanized technique needs to reduce labor hours , and the cost of agricultural implements and machineries. In other words the labor-saving mechanized technique should be developed based on the improvement of total farming system as well as systemic fundamental innovation of cultural methods. The efficiency of solar energy use in food production of Korea in 1997 is as low as $0.52{\%}$ so there is much room to increase yield. It is recommendable that the concept of food Production should be changed to energy Producing efficiency Per unit area basis from volume and weight of food materials. Moreover, introduction of resonable cropping system is needed to increase yield of main crops, farmer's income, solar energy use efficiency, and decrease of land service expenditure. Current cropping system emphasized on economic crops. especially in vegetables , is not desirable for resonable use of arable land. stability of agricultural management and staple food crop self-sufficiency ratio. It is desirable to increase food crops . that are energy of carbohydrate and protein rich and land dependent crops. in cropping system. And the agronomist should develop the cultural methods to replace food crops for food self-sufficiency and stable farming management instead of economic crops in current cropping system. Low-input and environmentally-sound crop cultivation techniques, especially nitrogen-reducing culture technique which is directly related to food crop quality, also needs to be developed urgently. The extended cultivation of corn in upland and barely and wheat in lowland as a feed stuffs is recommended to prevent further decrease of food self-sufficiency ratio, which is mainly caused by the high reliance on imported feed grain. It is also considered that the calculation and presentation methods of standard agricultural income needs to be improved. The current calculation method uses unit land area of 10a regardless of crop kinds , characteristics of agricultural management and cultivation scale. So, it is apt to lead misunderstanding of farm income value. Therefore. it should show an income of average farmers for certain number of years. Research and developing system for food producing is not desirable because they are conducted currently individual crop and mono-culture basis. But actual agricultural income is usually earned by cropping system including upland and lowland. For example. the barley and wheat is usually cultivated in double cropping system. The cooperation among research institutes such as university agribusiness. government and farmers is indispensible. The public information and education on importance and consumption habit of food crops is necessary in Korean society to increase food self-sufficiency through nationwide cooperation.

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Effect of Several Additives on Medium Browning and Mericlone Growth of Temperate Cymbidium Species (배지내 몇가지 첨가물질이 온대산 Cymbidium속 유묘배양시 배지산화 및 생육에 미치는 영향)

  • Chung, Jae-Dong;Lee, Jee-Hee;Jee, Sun-Ok;Kim, Chang-Kil
    • Horticultural Science & Technology
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    • v.16 no.2
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    • pp.239-241
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    • 1998
  • The experiments were carried out to improve culture efficiency of rhizome and mericlone propagation through settlement of problems occurring during culture period of temperate Cymbidium species. Shooting efficiency from rhizome of C. forrestii 'Nokwoon' was improved, when cultured in $H_3P_4$ medium (Hyponex 3+peptone 4g/L) supplemented with 170mg/L $NaH_2PO_4{\cdot}H_2O$ and 0.4mg/L Thiamin e HCl, but the other varieties were not influenced to shooting efficiency by additives. Medium in which rhizome of C. nishiuchianum 'Hodukjiwha' was cultured became less browned in $H_3P_4$ medium added with 150mg/L PVP, but the other treatments of antioxidants was failed to prevent the medium browning. Re-formation of rhizome from young shoots of C. forrestii 'Sojub', 5.5cm in length occured in $H_3P_4$ enriched with 2.0 mg/L NAA and 1.0 mg/L BA under darkness, but axillary buds were elongated in the medium with 1.0 mg/L NAA and 3.0 mg/L BA under light condition. On the other hand, rhizomes from young shoot of C. forrestii 'Seosinmae' and 'Songmae', 5.5cm and 2.5cm in length respectively were reformed in 2.0 mg/L NAA and 5.0mg/L kinetin under darkness, but multishoot from young shoot were emerged in 2.0mg/L NAA and 3.0mg/L BA.

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A Study on Safety Management Efficiency for Coastal Accident Prevention (연안사고 예방을 위한 효율적 안전관리 방안 연구)

  • Yun, Byungdoo
    • Journal of the Korean Society of Marine Environment & Safety
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    • v.26 no.7
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    • pp.777-786
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    • 2020
  • This study examines the efficiency of safety management measures taken in coastal waters to prevent coastal accidents. Recent years have witnessed about 800 cases of large and small coastal accidents in coastal waters annually. On average, these accidents have been responsible for 129 deaths annually, Thus, there is a need for effective preventive measures. Accordingly, the Korea Coast Guard enacted the Coastal Accident Prevention Act in 2014 and has cooperated with related organizations, such as local governments and the Ministry of Oceans and Fisheries, to safely manage coastal waters. However, compared to the rapid increase in human coastal activities, coastal accidents continue to occur due to the lack of a safety mindset among the Korea Coast Guard, a lack of public safety awareness, a lack of safety management facilities, and poor interest from related organizations, among others. This study examines the conditions and problems faced with regard to safety management in coastal waters based on recent coastal accident data, and analyzes foreign coastal waters safety management cases. Various methods to improve the safety management efficiency in coastal waters are proposed, such as enhancing the safety mindset and capabilities of the Korea Coast Guard, strengthening cooperation among related organizations, increasing the role of local governments, expanding and strengthening safety management facilities by applying new technologies, inculcating a culture of maritime safety, and activating private-public collaborative governance.

BIM Energy Efficiency Plan for Verification of Building Envelop Energy Code of Housing in USA - Based on the NYC Energy Conservation Code - (미국 공동주택의 건물 외피 에너지코드 검증을 위한 BIM 에너지 계획 방안 -뉴욕시 에너지 코드를 기준으로-)

  • Heo, Jinwoo
    • The Journal of the Convergence on Culture Technology
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    • v.8 no.4
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    • pp.313-322
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    • 2022
  • The number of architects who adopt BIM(Building Information Modeling) as the design method are gradually increasing because of its productivity and efficiency. Climate Change and Global Warming lead to legislation of new energy regulations and strengthen existing ones. The current architectural design methods (2D CAD) take a lot of time and effort to verify energy codes and are hard to adjust according to the design changes. The purpose of this study is to show the effectiveness of the BIM in building envelop energy modeling of the housing project. In the process of design method change, We could contribute to increasing productivity and efficiency in building energy verification through BIM because the updated value could be calculated simultaneously without information omission or recalculation process. The procedure for the study is as follows. Using BIM of the Goldin at Essex Crosing Housing Project by Revit 2011 as a case model, this study analyze the criteria for energy plan to conform to the energy code in NYC. The result value from the setting of the Revit model is compared with the reference value required by the NYC Energy Code. Finally, the data from BIM are entered into COMckeck, the energy verification program provided by U.S. Department of Energy, to check whether the building envelope energy performance conforms to NYC Energy Code.

An Empirical Study on the Impacts of the Team-Based Organization Characteristics on Team Effectiveness in the University Library (대학도서관 팀제 운영방식이 팀 효과성에 미치는 영향)

  • Yoon, Hye-Young
    • Journal of Korean Library and Information Science Society
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    • v.35 no.4
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    • pp.23-40
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    • 2004
  • This study examined the impact of team characteristics(introduction of the team approach team empowerment, leadership of team leaders, open communication director's commitment) on team effectiveness (adaptability to the environments, job satisfaction the positive team culture, cost efficiency) in university libraries. The study used a survey design with a questionnaire to staffs who worked at f academic libraries in South Korea. The major findings from the empirical analysis are as follows. The well-prepared introduction of team approach and director's strong commitment were positively related to the team effectiveness variables such as adaptability to environment, job satisfaction, and cost efficiency.

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An Investigative Study on the Structural Characteristics of High-Rise Complexes in Korea (국내 고층 주상복합 건물의 구조적 특성에 관한 조사 연구)

  • Kang Suk-Won;Lee Sung-Ho
    • Korean Journal of Construction Engineering and Management
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    • v.4 no.4 s.16
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    • pp.137-144
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    • 2003
  • Recently, the interest in a complex building increases rapidly in Korea. High-rise complex is not simply the combination of a residential apartment and a business office as previous but a building with the object of the convenience of the residents familiar with city-life style through adapting the high class life style and new residential culture to the business space, and the efficiency in using the limited building site in the town. This study presents an exploratory analysis focusing the structural system with the help of the extensive survey of the construction site in Korea, and aims a guideline for the structural design and construction of high rise complex. Through the survey, change and development in the design and the construction can be seen as the height and size of the complex grow. It is almost indispensable to design a structural system against lateral forces like earthquake or wind, which is usually measured by story drift ratio or story displacement. Improvement of the structural materials and their usages is also included for the efficiency of the structural system. Useful slab-beam system contributing to the decrease of the story height is still a concern.