• Journal of Embryo Transfer
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• v.27 no.2
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• pp.71-80
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• 2012

Biotechnologies for cloning animals and in vitro embryo production have the potential to produce biomedical models for various researches. Especially, pigs are a suitable model for xenotransplantation, transgenic production and various areas of reproductive research due to its physiological similarities to human. However, utilization of in vitro-produced embryos for transfer remains limited. Despite improvement over past few decades, obstacles associated with the production of good quality embryos in vitro still exist which limit the efficiency of cloning. One of major problems includes improper in vitro maturation (IVM) and culture (IVC). Oxidative stress caused from in vitro culture conditions contributes to inadequate IVM and IVC which leads to poor developmental competence of oocytes, failure of fertilization and embryo development. To reduce the oxidative stress, various antioxidants have been used to IVM and IVC system. However, limited information is available on the effects of resveratrol on livestock reproductions. Resveratrol is a polyphenolic natural product and well known as an antioxidant in foods and beverages (e.g. in grapes and red wine). Resveratrol is known to be cardioprotective, anticarcinogenic, anti-inflammatory, antioxidant and antiapoptotic. This paper will review the effects of resveratrol on in vitro maturation of oocytes and embryo development.

• The Research Journal of the Costume Culture
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• v.20 no.4
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• pp.535-548
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• 2012

The purpose of this study is to research the achievement motivation of beauty industry professionals. The research method involved one-on-one, in-depth interviews with 7 hair professionals, 7 make-up professionals and 7 skincare professionals. The in-depth interviews were recorded in their entirety, and the recorded content was transcribed and organized. Analysis of the transcribed data involved encoding, deconstructing and re-composing the interview content. Research results revealed 5 factors that were extracted to explain the achievement motivation of beauty industry professionals. Four of the 5 factors were similar to the results of precedent studies, but the remaining factor presented unique characteristics. The 4 common factors were "motive to pursue success", "motive to avoid failure", "motive to meet challenges" and "goal-oriented motive". The factor discovered in this study was "relationship-oriented motive", which is characterized by the tendency to place importance in relationships with superiors, colleagues and customers based on the working characteristics of beauty industry professionals. Based on the results of this study, beauty industry professionals can learn about goals that must be met in various stages in order to establish a system for accomplishing those goals, implement an emotional education program for mental balance, introduce a competitive team system, and carry out a relationship improvement program to increase work efficiency.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.13 no.10
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• pp.4786-4793
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• 2012

In a recent mobile computing environment, the research for a transaction model have been actively carried out to deal with real-time transaction. However, the research for recovery model is relatively insufficient. Therefore, it would be very important for mobile recovery methods for database stability, just in case of data loss due to hand-off in mobile computing system. So, further efficient research should be keenly done to reduce I/O hand-off in mobile computing systems. SLL method increases I/O time due to sequential file. This paper have been suggested to efficient mobile recovery method in mobile computing system. Finally, it has been clearly demonstrated for system efficiency, conducting the performance assessment that currently proposed recovery system.

• International Journal of Advanced Culture Technology
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• v.8 no.1
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• pp.165-172
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• 2020

Industrial Revolution means the improvement of productivity through technological innovation and has been a driving force of the whole change of economic system and social structure as the characteristic of technology as the tool of this productivity has changed. Since the first industrial revolution of the 18th century, productivity efficiency has been advanced through three industrial revolutions so far, and this fourth industrial revolution is expected to bring about another revolution of production. In this study, the demand for the introduction of artificial intelligence(AI) technology has been increasing in various business fields due to the rapid development of ICT technology, and the classification of HS(harmonized commodity description and coding system) items has been decided using artificial intelligence technology, which is the core of the fourth industrial revolution. And it is enough to construct HS classification system based on AI technology using inference and deep learning. Performing the HS item classification is not an easy task. Implementation of item classification system using artificial intelligence technology to analyze information of HS item classification which is performed manually by the current person more accurately and without any mistake, And the customs administrations, customs offices, and customs agencies, it is expected to be highly utilized in the innovation of trade practice and the customs administration innovation FTA origin agent.

• Journal of the Korean Solar Energy Society
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• v.26 no.3
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• pp.1-8
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• 2006

The objective of this research is to study on the analysis of long-term performance characteristics of various solar thermal system for heating protected horticulture system for reducing heating cost, increasing the value of product by environment control, and developing advanced culture technology by deploying solar thermal system. Long term field test for the demonstration was carried out in horticulture complex in Jeju Island. Reliability and economic aspect of the system which was operated complementary with thermal storage and solar hot water generation were analyzed by investigating collector efficiency, operation performance, and control features. Optimum operating condition and its characteristics were closely investigated by changing the control condition based on the temperature difference which is the most important operating parameter. However, it is expected that, in high-insolation areas where large-scale ground storage is adaptable, solar system demonstrated in the research could be economically competitive and promisingly disseminate over various application areas.

• Journal of Microbiology and Biotechnology
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• v.6 no.6
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• pp.451-455
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• 1996

Physiological studies on the expression of Bacillus thuringiensis subsp. tenebrionis (Btt) gene coding for insecticidal protein in recombinant Escherichia coli 537 were carried out to identify optimal culture condition. It was necessary to shift culture temperature from 30 to $42^{\circ}C$ to express the gene. Expression of the Btt toxin gene by recombinant E. coli 537 began within one hour after induction. Complex nitrogen sources increased production of the insecticidal protein. The total insecticidal protein was 0.5 g/I when using yeast extract as a complex nitrogen source. Soybean hydrolysate showed apparently the highest induction efficiency. After induction, the cellular content of the insecticidal protein was 5.4 times higher than it had been before induction. The optimal cultivation strategy was found to grow cells for 7hours at $30^{\circ}C$ and then 5-8 hours at $42^{\circ}C$. The optimal cultivation pH for the production of insecticidal protein was 6.5. The Btt toxin produced by the recombinant E. coli 537 was found to have the same level of potency against Colorado potato beetle as the original toxin.

• International Journal of Quality Innovation
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• v.9 no.2
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• pp.11-30
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• 2008

The purpose of this study is to develop BSC model of social enterprise. Performance analysis tool of BSC have been brought over from the business world, designed and created from the perspectives of profit-based businesses. The BSC is a strategic performance measurement and management tool designed for the private sector acting as a communication/information and learning system, to measure 'where we are now' and 'where to aim for next'. It prescribes a plan for translating 'vision' and 'strategy' into concrete action across four perspectives at different stages, depending on the business. These perspectives are 'financial', 'customer', 'internal processes' and 'learning and growth', each of which is connected by cause-and-effect relationships that reflect the firm's strategy. Social aims of social enterprise are to accomplish desired outcomes which are to employ vulnerable people and to provide social services. The measurement factors of financial perspective are stable funding, efficiency of budgeting, stakeholders' financial supports, and trade profit. The measurement factors of customer perspective are government, social service users, employees, local communities, sup plier, social activity company, and partnership with external organizations. The measurement factors of internal process perspective are organizational culture, organizational structure/management, internal/external communication, quality of products and services, information sharing. The measurement factors of learning and growth perspective are training and development, management participation, knowledge sharing, leadership of CEO and manager, and learning culture.

• Plant Biotechnology Reports
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• v.2 no.3
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• pp.219-226
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• 2008

Transgenic plants with the herbicide-resistance gene (bar gene) were obtained via organogenesis from isolated mesophyll protoplasts of Nierembergia repens after applying electroporation. Transient ${\beta}-glucuronidase$ (GUS) activity of electroporated protoplasts assayed 2 days after applying an electric pulse showed that optimum condition (transient GUS activity 319 pmol 4 MU/mg per min and plating efficiency 2.43%) for electroporation was 0.5 kV/cm in field strength and $100{\mu}F$ in capacitance. The protoplasts electroporated with the bar gene at this condition initiated formation of microcolonies on medium after 2 weeks. After 4 weeks of culture, equal volume of fresh 1/2-strength Murashige and Skoog (MS) medium containing 0.2 mg/l bialaphos was added for selection of transformed colonies. After 6 weeks of culture, growing colonies were transferred onto regeneration medium containing 1.0 mg/l bialaphos, on which they formed adventitious shoots 1-2 months after electroporation. The adventitious shoots rooted easily after transfer onto MS medium with bialaphos lacking plant-growth regulators. Transformation of these regenerants with the bar gene was confirmed by Southern analysis. Some of the transformants showed strong resistance to the application of bialaphos solution at 10.0 mg/l.

• Korean Journal of Microbiology
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• v.30 no.3
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• pp.149-153
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• 1992

Cloning of the gene encoding extracellular .betha.-lactamase from Streptomyces sp. SMF13 in a plasmid pIJ702 and expression of the gene in Streptomyces invidans were carried out. Optimal conditions for the formation of protoplasts of S.lividans and the regeneration of the protoplasts were evaluated. Streptomyces sp. SMF-13 was selected as a donor strain of .betha.-lactamase gene and totla DNA of the strain was partially digested with Sau3A I. DNA fragments ranged from 4kb to 10 kb were ligated to pIJ702 AT Bgl II site and then the ligated DNAs were transformed to the protoplasts of S, livivans. The transformation efficiency was $2 *10^{3}$ .$\mu$g DNA for the ligated DNA mixture. One colony among a thousand colonies regenerated showed extracellular .betha.-lactamase and the size of the inserted DNA fragment was estimated to be 3.94 kb. The .betha.-lactamase activity in the culture broth of the recombinant strain was maximum at 3 days culture to be 1.0 unit/ml.

• Journal of Environmental Health Sciences
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• v.22 no.2
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• pp.104-113
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• 1996

Cadmium uptake by growing and nongrowing (intact) cells of a chdmium-tolerant yeast Hansenula anomala B-7 in the presence of surfactants was studied. In growing cultures the addition of Triton X-100 or Tween 80 increased cadmium uptake by about 30% with no inhibition of cell growth, and in intact cells Triton X-100 increased cadmium accumulation by about 80% compared to surfactant-free controls. Considering balance between increased uptake and pollution, the addition of 0.1% Triton X-100 was preferable. By the mixed addition with defoamer silicone, during growth of cells Tween 80 or Triton X-100 enhanced uptake efficiency of cadmium compared to its single addition, whereas in intact biomass each of surfactants tested had no significant effect on cadmium uptake. The uptake of cadmium was observed to rise sharply to a maximum and then declined with increasing pH, and maximum accumulation of cadmium by growing and intact cells occurred at the pH of 6.0 and 7.0, respectively. A significant increase in cadmium uptake occurred with shaking culture. Cadmium uptake by growing and intact cells was almost completed during the culture time of 72 or 24 hrs, respectively. Scalded cells sorbed much more cadmium-ion than living cells.