• Title/Summary/Keyword: Culture efficiency

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Efficient Derivation of New Human Embryonic Stem Cell Lines

  • Kim, Sun Jong;Lee, Jeoung Eun;Park, Jong Hyuk;Lee, Jung Bok;Kim, Jin Mee;Yoon, Byung Sun;Song, Ji Min;Roh, Sung Il;Kim, Chul Geun;Yoon, Hyun Soo
    • Molecules and Cells
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    • v.19 no.1
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    • pp.46-53
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    • 2005
  • Human embryonic stem (hES) cells, unlike most cells derived from adult or fetal human tissues, represent a potentially unlimited source of various cell types for basic clinical research. To meet the increased demand for characterized hES cell lines, we established and characterized nine new lines obtained from frozen-thawed pronucleus-stage embryos. In addition, we improved the derivation efficiency from inner cell masses (to 47.4%) and optimized culture conditions for undifferentiated hES cells. After these cell lines had been maintained for over a year in vitro, they were characterized comprehensively for expression of markers of undifferentiated hES cells, karyotype, and in vitro/in vivo differentiation capacity. All of the cell lines were pluripotent, and one cell line was trisomic for chromosome 3. Improved culture techniques for hES cells should make them a good source for diverse applications in regenerative medicine, but further investigation is needed of their basic biology.

Fish Diseases and Their Control in High Density Culture of Eel (고밀도 뱀장어 양식수조의 질병대책)

  • CHUN Seh Kyu
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.16 no.2
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    • pp.103-110
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    • 1983
  • The main purpose of the present study is to evaluate a revolving plate type biofilter system for mass culture of eel(Anguilla japonica) based on the experimental rearing for 120 days (Oct. 1982-Feb. 1983). Water quality changes, growth efficiency of fish and fish disease treatment were critically evaluated. A revolving plate type biofilter system was designed(Fig. 1). The system consisted of a glass tank (150 l), a revolving plate biofilter and a settling tank(150 l). The biofilter consisted of 60 submerged quadriangular plates ($28{\times}37$ cm) and 30 revolving plates (32 cm diameter) for a total of 19.0 $m^2$ of surface area. The revolving plates were made to rotate 10 time per minute, The total water volume of the rearing system were 300 l, and everyday 1/3 of the total water volume were changed with freshly prepared water. In the rearing system a total of 2 kg of eel (1,500 individuals, mean weight:1.3 g) were reared fed on the pellet feed and the dough feed. The growth efficiency were much better for the pellet feed (FC: 1.79) compared to the dough feed (FC: 3.56). During the experimental rearing water quality control was satisfactory. Total ammonia concentrations were 0.38-0.59 ppm and nitrite concentration were 0.83-1.19 ppm. On the other hand alkalinity decreased from 176ppm just after the water change to 17ppm just before the water change. The low alkaline condition was compensated by the regular change of water. Epidemics of parasitic gill-flocks (Pseudodactvlogylus sp.) was observed, and they were easily eliminated by the treatment of DDVP (1.0 ppm). Trichodina sp. and Costia sp. were observed, and they were also controlled by the treatment of potassium permanganate (4.0 ppm).

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A Study on Design for Casual Look Applying Painting Images of Henri Matisse (앙리 마티스 회화 이미지를 응용한 캐쥬얼 룩 디자인 연구)

  • Sim, Mi-Jung;Yu, Kum-Wha
    • The Research Journal of the Costume Culture
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    • v.18 no.4
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    • pp.612-625
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    • 2010
  • People have more expectations for arts and design and accordingly, various artworks were combined with fashion to meet the consumer needs. As people live a decent life, the development of leisure activities and industry has a much effect on apparel. With this phenomenon, a free and active casual look is making progress centering around the practical apparel, pursuing diversified efficiency irrespective of a season and considering sensibility not formality. In this study, paintings of Henri Matisse were analyzed in every respect and were applied in apparel design with diverse color arrangement and a motif originating in the phenomenon of modern fashion design which leads to the integration of arts and design. Painting image and color of Henri Matisse were used. Sportive casual and cultural casual was used in design as well. Originality of its color in the paintings which were used an a motif is coming from Gauguin and Gogh. Henri had influenced to the next generation with pursuit of violent color. The following conclusions were drawn from this study. First, the color of Henri Matisse's paintings has a strong contrast effect. It combines notable violent color with a simple yet decorative motif. Therefore color from Matisse's paintings suit for apparel of marked individuality with its free color arrangement. Second, free and active image in Henri Matisse's paintings is easy to express efficiency and popularity. It accords with the feature of casual wear. Third, through adding a flowing curved line in Henri Matisse's paintings to materials and applying various colors putting into a curved line image to a rib section, a decorative effect which goes with the whole shape is obtained. This study presents possibility of emergence of unique design using free color arrangement and motif from the image of paintings and aims development of modern fashion design in accordance with modern fashion giving importance to the difference and sensibility by integration of modern garments and artworks.

A Study on the Efficiency of Deep Learning on Embedded Boards (임베디드 보드에서의 딥러닝 사용 효율성 분석 연구)

  • Choi, Donggyu;Lee, Dongjin;Lee, Jiwon;Son, Seongho;Kim, Minyoung;Jang, Jong-wook
    • The Journal of the Convergence on Culture Technology
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    • v.7 no.1
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    • pp.668-673
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    • 2021
  • As the fourth industrial revolution begins in earnest, related technologies are becoming a hot topic. Hardware development is accelerating to make the most of technologies such as high-speed wireless communication, and related companies are growing rapidly. Artificial intelligence often uses desktops in general for related research, but it is mainly used for the learning process of deep learning and often transplants the generated models into devices to be used by including them in programs, etc. However, it is difficult to produce results for devices that do not have sufficient power or performance due to excessive learning or lack of power due to the use of models built to the desktop's performance. In this paper, we analyze efficiency using boards with several Neural Process Units on sale before developing the performance of deep learning to match embedded boards, and deep learning accelerators that can increase deep learning performance with USB, and present a simple development direction possible using embedded boards.

Effects of perceived attributes of fashion live streaming commerce (FLSC) on attitudes and intention to use (패션 라이브 스트리밍 커머스(FLSC)의 속성 지각이 태도와 이용의도에 미치는 영향)

  • Seo, Hyesim;Yoh, Eunah
    • The Research Journal of the Costume Culture
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    • v.30 no.2
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    • pp.297-318
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    • 2022
  • A growing number of fashion brands and retailers are actively adopting live streaming as a new marketing channel. In spite of the increasing use of live commerce, the effects of live streaming commerce on customer purchasing behavior for fashion products are not fully understood. The purpose of this study is to examine factors affecting consumers' attitudes toward a purchase via fashion live streaming commerce (FLSC) and intention to use FLSC. The study also investigated whether consumers' expenditure on fashion and time spent on mobile shopping moderate the relationships among research variables. A total of 230 questionnaires were analyzed through descriptive statistics, confirmatory factor analysis, and multiple-group comparison tests using SPSS and AMOS. A summary of the main results of this study is as follows. First, the perception of the attributes of FLSC (ease of use, economic efficiency, interactivity, and enjoyment) has a positive effect on attitude toward a purchase via FLSC. The ease of use and economic efficiency of FLSC, in particular, have greater impacts on attitudes than other factors. Secondly, attitudes toward FLSC positively impact the intention to use FLSC. Lastly, the results of group comparisons, by fashion expenditure and time spent on mobile shopping respectively, hold no significant moderation effects among the variables. These findings demonstrate that consumers are more likely to use FLSC as they form a positive attitude by the attributes of FLSC mentioned earlier. The study provides some insights on an exploration of live streaming commerce for fashion product sales.

Design of variable 4-bar linkage structure for adjustable driving angle (구동 각도 조절이 가능한 가변형 4절링크 설계)

  • Kim, Sang-Hyun
    • The Journal of the Convergence on Culture Technology
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    • v.8 no.5
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    • pp.591-596
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    • 2022
  • Since 4-bar linkage widely used in various industrial fields has a fixed link length, it is inconvenient to add an independent link structure or control device to change the movement of output link. Therefore, this paper proposes a new concept of variable 4-bar linkage mechanism to selectively adjust the movement of the output link to fit desired situations, and applied to the commercial table fans, which is a representative product using a 4-bar linkage system. The optimal rotation angle steps for efficiency are determined experimentally and the appropriate lengths of linkage to fit each step are calculated analytically. Changes in the linkage length are implemented by the rotational motion using a grooved cylindrical cam and the feasibility of the proposed variable linkage mechanism is verified through fabrication and measurement. The presented variable link mechanism is expected to improve the efficiency of industrial robots and fuel valve systems.

A comparison of the types and characteristics of the purchase channel journey of fashion products in the MZ generation (MZ세대의 패션상품 구매채널여정 유형화와 특징 비교)

  • Lee, Jung-Woo;Kim, Mi Young
    • The Research Journal of the Costume Culture
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    • v.30 no.5
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    • pp.656-674
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    • 2022
  • The purpose of this study is to reveal and compare the differences in the types and characteristics of purchase channel journeys of MZ generation consumers. In this study a survey was conducted on the purchase channel journey of 20 women in the MZ generation using the ethnographic method of in-depth interviews and observations. As a result, three purchase channel journeys were identified: mobile, multi-channel, and offline. These were variously subdivided according to the characteristics of the MZ generations. Gen Z's journey was categorized into types: fashion platform app, Youtube, multi-channel supplement, multi-channel non-planned store visit, offline loyalty store, and impulsive offline store. Gen M's journey was categorized as: an online community bond, portal site, online loyalty store, multi-channel brand involvement, multi-channel efficiency, a multi-channel conversion, offline efficiency and offline task. The difference in mobile journey between generations was found in the time and length of the purchase. Gen M recognized both online and offline search processes to be tiring, while Gen Z enjoyed the search process using the online path. In the offline journey Gen Z began with their own intention to purchase, while Gen M sometimes recognized that purchasing fashion products necessary for work was a cumbersome task.

Enhancing Retrieval Performance for Hierarchical Compact Binary Tree (계층형 집약 이진 트리의 검색 성능 개선)

  • Kim, Sung Wan
    • Journal of Creative Information Culture
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    • v.5 no.3
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    • pp.345-353
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    • 2019
  • Several studies have been proposed to improve storage space efficiency by expressing binary trie data structure as a linear binary bit string. Compact binary tree approach generated using one binary trie increases the key search time significantly as the binary bit string becomes very long as the size of the input key set increases. In order to reduce the key search range, a hierarchical compact binary tree technique that hierarchically expresses several small binary compact trees has been proposed. The search time increases proportionally with the number and length of binary bit streams. In this paper, we generate several binary compact trees represented by full binary tries hierarchically. The search performance is improved by allowing a path for the binary bit string corresponding to the search range to be determined through simple numeric conversion. Through the performance evaluation using the worst time and space complexity calculation, the proposed method showed the highest performance for retrieval and key insertion or deletion. In terms of space usage, the proposed method requires about 67% ~ 68% of space compared to the existing methods, showing the best space efficiency.

Reduction of Polyspermy in Porcine in vitro Fertilization by Modified Swim-UP Method

  • Park, C.H.;B.S. Koo;Kim, M.G.;J.I. Yun;H.Y Son;Lee, S.G.;Lee, C.K.
    • Proceedings of the Korean Society of Developmental Biology Conference
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    • 2003.10a
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    • pp.110-110
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    • 2003
  • The high incidence of polyspermic fertilization is one of the major causes lowering the overall efficiency of porcine IVF. The common procedure for IVF involves the co-culture of both gametes in the medium drop, which increases sperm concentration and incidence of polyspermy. Therefore, the present study was carried out to increase the efficiency of porcine IVF by reducing polyspermy using a modified swim-up method. This method modifies conventional swim-up washing by placing oocytes directly at the time of washing. Sperm pellet was prepared in the tube and mature oocytes were placed on cell strainer with $70 \mu m$ pore size (Falcon 2350) at the top of the tube. After insemination, the oocytes were stained for examination. Also, the developmental potential of fertilized embryos was measured to evaluate for the feasibility of this method. While having similar penetration rates in both methods ($86.67 \pm 2.36% to 83.33 \pm 1.36%$), there was a significant reduction of polyspermy in modified swim-up method ($17.50 \pm 1.60%$) compare to the control ($44.1 \pm 3.70%$ (p<0.05). Subsequent culture showed higher rate of blastocyst formation in modified swim-up method (20.44$\pm$0.99%) than the control ($15.73 \pm 3.26%$) (P<0.05), even though there was no significant difference. These results suggest that, by controlling the number of spermatozoa reaching the oocytes, porcine oocytes might be protected from polyspermy in vitro. Also, the developmental potential of the fertilized embryos using this method could be improved by increasing the pool of spermatozoa with better quality. Further optimization of the procedure required to implicate this method in routine porcine IVF.

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Experimental Vrification of the Sray Clculation using the Aricultural Done (농업용 방제드론의 방제면적 산출에 따른 실험적 검증)

  • Wooram Lee
    • The Journal of the Convergence on Culture Technology
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    • v.9 no.4
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    • pp.569-576
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    • 2023
  • An agricultural drones are gradually increasing in utilization due to economic efficiency, and consist of a main frame in charge of flying spray system in charge of moving pesticide to control targets. Therefore, the environment and characteristics of crops should be considered when controlling pesticides using drones and conditions such as systematic flying altitude of flight, speed, and spray time should be changed accordingly. However, pest control work using agricultural drones has different spray effects depending on level the operation proficiency and spray impact. In addition, there are variations in operating standards and control efficiency for agricultural drones, which hinder the distribution of agricultural control drones in the field of pest control work. Therefore, this study attempts to identify the spraying characteristics of agricultural drones, apply the effective spraying time, interval and experimentally verify the system that can calculation of spray area compared to previous studies. Through this experimental verification, it is intended to apply the optimal control process by minimizing the obstacles to pest control work by applying the operation method and systematic figures to agricultural drones.