• 한국수산과학회지
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• 제37권4호
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• pp.287-294
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• 2004

Effect of growth and survival rate on different densities in the cage culture of juvenile abalone (Haliotis discus hannai) were determined in Myoduri Yeosu, Jeollanamdo from April 2000 to April 2001. The shell length growth was conducted using $32.35{\pm}1.35$ mm abalone juveniles for 374 days at densities $15{\%}\;(230\;indv./m^{2}),\;30{\%}\;(460\;indv./m^{2}),\;45{\%}\;(690 \;indv./m^{2}),\;60{\%}\;(920\;indv./m^{2})$. The result showed that the hightest growth rate was observed in lowest $(15{\%})$ density experimental group. Survival rate of Juvenile abalone was the highest in $15{\%}$\; density group and lowest in $45{\%}$ density group and distribution rate of shell length showed the highest as $30{\%}\;in\;230\;indv./m^{2}\;(15{\%})$ group.

• 복식문화연구
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• 제9권5호
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• pp.759-769
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• 2001

The principal purpose of this study is focused on the fabrics used in Kaya period by investigating the imprinted fabrics on the remains of the old tomb of Dohang-ri and Jeesan-dong which were built around the late 4th century to the late 6th century. Most of the remains are plain weaved, however there is one remain that 4-end complex gauze with patterned, and the other is weaved with geometrical pattern which were plain weave with 2 · 1 twill and 3 · 1 twill. The imprinted fabrics that had twists had S twists only. Of all the fabrics that were examined, the most loose one had the density of 8 × 6/cm, the most fine fabric had the weft density of 91.8 strands/cm and the warp density cannot be counted. It is No. 416 of Doharg-ri tomb that has the highest ratio of density as 3.15. All the fabrics observed by SEM and Polarizing Microscope are hemp.

• 한국양식학회지
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• 제18권1호
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• pp.19-25
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• 2005

본 연구는 P. nana의 집약적인 대량배양을 위한 최적 배양밀도를 규명하기 위한 것으로 nauplius 생산과 성체생산의 두 가지 측면에서 조사되었다. 2 ml 배양규모에서 성체 암컷 밀도에 따른 성체 암컷의 nauplius 생산력은 성체 암컷의 밀도가 증가함에 따라 감소하는 경향을 보였지만 copepodid와 nauplius의 밀도는 성체 암컷의 생산력에 영향을 미치지 않는 깃으로 나타났다 8 L배양 용량에서 일주일 동안 암컷의 일일 평균 생산량은 1, 3, 5, 7개체/ml실험구에서 각각 0.6, 1.1, 17, $2.3{\times}10^5$ 개체로 배양밀도가 증가할수록 생산량이 증가하였으나 10 개체 /ml로 배양한 실험구는 $1.3\times10^5$개체로 7 개체/ml로 배양한 실험구보다 낮은 생산량을 보였다. 5 L배양용기에서 15일 동안 P. nana nauplius의 접종 밀도에 따른 생존개체는 모든 실험구가 시간이 경과함에 따라 감소하는 경향을 보였으며, 15일째, C4-성체까지의 생존율은 50, 100, 150및 200개체/ml 실험구에서 각각 32.7, 307, 28.9 및 $23.0\%$로 배양밀도가 증가함에 따라 감소하는 경향을 보였으나 유의적인 차이는 없는 것으로 나타났다. 그러나 250 개체/ml와 300 개체/ml 로 배양한 실험구는 각각 $19.7\%$와 $18.4\%$로 50, 100, 150 개체/ml 실험구보다 낮은 생존율을 보였다. P. nana성체의 nauplius에 대한 공식은 있는 것으로 나타났으며 먹이의 공급 시 공식이 줄어드는 것으로 판단된다. 결과적으로 P. nann는 성체 생산을 위한 nauplius의 최대 접종밀도가 200개체/ml인 점을 고려해 볼 때, 비교적 고밀도로 배양이 가능하며 어류 종묘생산장에서 먹이 생물로 사용이 가능할 것으로 보인다.

• Journal of Plant Biotechnology
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• 제36권1호
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• pp.7-12
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• 2009

본 실험은 음나무 배발생 세포의 증식 및 체세포배 발달을 위한 액체 현탁 배양조건의 확립을 위해 수행되었다. 배발생 세포의 생장율은 접종 밀도가 증가할수록 감소하였다. 배발생 세포의 증식에 가장 효과적인 접종 밀도는 0.1 g/100 ml 로서 이 농도에서 세포의 생장율이 가장 높았다. 배양기간에 따른 배발생 세포의 생장 패턴 및 세포 주기 (G1, S, G2/M) 분석 결과, 세포의 생장은 배양 5일 후부터 증가가 시작되어 15일 까지 급격히 생장하였으며 그 이후에는 점차 감소하였다. 배양 기간 별 세포 주기 (cell cycle)의 변화가 명확하게 관찰되어 배양 5일째 5기는 초기의 5.5% 에서 11.7%로 두 배정도 증가하였으며, 배양 15일 이후부터는 다시 초기의 세포 주기로 되돌아가면서 안정화되는 것으로 나타났다. 따라서 음나무 배발생 세포의 현탁배양은 15일의 주기로 배양하는 것이 증식에 가장 효과적인 것으로 생각되었다. 한편 배발생 세포에서 체세포배의 유도는 배양초기의 접종 밀도가 중요한 것으로 나타났다. 0.5 g/L 의 낮은 밀도로 접종 시에는 65% 이상의 어뢰형 배가 유도된 반면 접종 밀도가 높아질수록 어뢰형으로의 배발달은 급격히 감소하였다. 초기의 접종 밀도가 증가할수록 특히 어뢰형 배의 발달은 지연되었으나 구형 및 심장형 배의 유도는 초기 접종밀도에 영향을 받지 않았다. 이상의 실험결과로 음나무 액체 배양 시 초기 접종 밀도를 조절함으로써 배발생 캘러스의 증식 및 체세포배를 효과적으로 유도할 수 있었으며 이는 체세포배 생산을 위한 배양 기간의 단축이 가능함을 보여주는 결과이다.

• 한국식생활문화학회지
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• 제11권1호
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• pp.53-59
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• 1996

This study was conducted to standardize the cooking methods of Omija-Pyuns and to investigate the effect of different starch densities (6% 7%, 8%, 9%) on the texture and taste of Omija-Pyuns. The results of these studies were as follows: 1) It was better for Omija to soak for 18 hours. The most favorite Mungbean starch density of 7% of Omija-Pyuns has the degree of lightness of 18.90, the degree of redness of 19.18, and the degree of yellowness of 3.98. 2) The Brix of syrup used for Omija-Pyuns was 49.4%. 3) As a result of the measurement of texture through Instron, if Mungbean starch density is increased, its hardness also increase. Each of the Omija-Pyuns (6%, 7%, 8%, 9%, in density) has showed its effection (p<0.05). 4) The result of the sensory evaluation showed that the Omija-Pyuns produced with Mungbean starch density of 7% was most preferred in appearance and overall taste, but it did not show the statistical effection (p<0.05) from the Omija-Pyuns produced with Mungbean starch density of 8%. The flavour was highest in Omija-Pyuns of 6%, and as the density was increased, the flavour decreased.

• KSBB Journal
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• 제14권2호
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• pp.160-166
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• 1999

본 연구에서는 ($\beta$-carotene 생산균주인 미세조류 Dunaliella bardawil을 사용하여 batch flask에서 미세조류의 고농도세포에 관한 최적배양조건(미량원소, pH, agitation speed, nitrate, phosphate, carbon source)을 확립하고자 하였다. 미량원소는 5X 배지에서 교반하였을 때 비생장속도는 $0.0l3hr^{-l}$와 세포농도는 $4.9{\times}10^6$ cells/mL로서 IX. 3X, lOX 배지에서 배양한 것보다. 약 46%, 18%, 69% 높은 세포수율을 얻었으며 세포배양시 교반한 경우, pH는 80에서 최대 세포농도를 얻었다. 초기 nitrate ($KNO_3$)와 phosphate($KH_2PO_4$)의 영향을 조사한 결과 미세조류 생장에 중요한 영양분으로서 질소원의 주입은 매우 효과적임을 확인하였다. 또힌 탄소원으로서 250mM의 $NaHCO_3$와 $CO_2$ 가스를 동시에 사용한 배양조건이 500mM $NaHCO_3$만을 탄소원으로 사용한 실험에 비하여 32% 증가된 세포농도를 나타내었다. light는 white light의 경우 blue light보다 세포생장에 적합하였다. 질소원을 이용한 유기배양시 2회의 nitrate주입만으로써 배양 198hr에 $8.955{\times}10^6$cell/mL의 고농도의 세포를 얻었다.

• Biotechnology and Bioprocess Engineering:BBE
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• 제5권2호
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• pp.106-109
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• 2000

Vascular endothelial cells (EGs) are usually difficult to culture to culture in a large scale because of their complicated requirements for cell growth. As the vascular endothelial growth factor (VEGF) is a key growth factor in the EC culture, we transfected human umbilical vein endothelial cells (HUVEC) using a plasmid containing VEGF gene and let them grow in a culture medium eliminated an important supplement, endothelail cell growth supplement(ECGS). The expression of VEGF by HUVEC tansfected with Vegf GENE was not enough to stimulate the growth of HUVEC, only 40% of maximum cell density obtainable in the presence of ECGS. However, when the culture medium was supplied with 2.5 ng/ml of basic fibroblast growth factor (bFGF), a synergistic effect effect of VEGE and bFGF was observed. In this case, the final cell density was recovered was recovered up to about 78% of maxium value.

• 동의생리병리학회지
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• 제22권4호
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• pp.771-777
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• 2008

Chungpaesagan-tang which is used for treating patients of brain in cerebrovascular disease frequently from clinical doctor has not reported about the effect of neuronal aptosis caused of brain ischemia. The aim of this study is to investigate effect of Chungpaesagan-tang protecting neuronal cells from being damaged by brain ischemia through using organotypic hippocampal slice cultures. We caused ischemic damage to organotypic hippocampal slice cultures by oxygen and glucose deprivation. And added Chungpaesagan-tang extract to cultures. thereafter we measured area percentage of propidium iodide (PI)-stained neuronal cell, lactate dehydrogenase (LDH) levels in culture media and Terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL)-positive cells. Area percentage of PI-stained neuronal cells and count of TUNEL-positive cells in CA1 and DG area of organotypic hippocampal slice culture were significantly decreased in pertinent density level of Chungpaesagan-tang extract. LDH levels in culture media of organotypic hippocampal slice culture were significantly decreased in pertinent density level of Chungpaesagan-tang extract. Within pertinent density level, Chungpaesagan-tang has cell protection effect that prevents brain ischemia damaging neuronal cells and apoptosis increasing.

• 대한한방내과학회지
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• 제29권1호
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• pp.231-242
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• 2008

Objectives : We can find out the experimental reports of Yanggyuksanhwa-tang, which has the function of regulating blood pressure related with cerebral disease, and increasing local cerebral blood stream volume, also has the recoveries for the damage of vessel endothelium, and endothelium hypertrophy caused by angiospasm after subarachnoid hemorrhage, and reduces the contraction of smooth muscle, so simultaneously improves necrosis. The aim of this study is to investigate effect of Yanggyuksanhwa-tang protecting neuronal cells from being damaged by brain ischemia through using organotypic hippocampal slice cultures. Methods : We caused ischemic damage to organotypic hippocampal slice cultures by oxygen and glucose deprivation, and Yanggyuksanhwa-tang extract was added to cultures. Thereafter we measured area percentage of propidium iodide (PI)-stained neuronal cell, lactate dehydrogenase (LDH) levels in culture media and terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL)-positive cells. Results : Area percentage of PI-stained neuronal cells and count of TUNEL-positive cells in CA1 and DG area of organotypic hippocampal slice culture were significantly decreased in pertinent density level of Yanggyuksanhwa-tang extract. LDH levels in culture media of organotypic hippocampal slice culture were significantly decreased in pertinent density level of Yanggyuksanhwa-tang extract. Conclusions : Within pertinent density level, Yanggyuksanhwa-tang has cell protection effect that prevents brain ischemia damaging neuronal cells and apoptosis increasing.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2000년도 추계학술발표대회 및 bio-venture fair
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• pp.338-341
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• 2000

An internal membrane bioreactor system was employed for continuous succinic ac id production from glucose in order to prove its performance and practicality. Succinic acid-producing Anaerobiospirillum succiniciproducens required more $CO_2$ for the proper growth and succinic acid production in cell recycled continuous culture than in batch culture. The maximum productivity obtained in cell recycled continuous culture was about 3.3 g/L-h which was ca. 3.3 times higher than that obtained in batch culture.