• KSBB Journal
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• 제32권2호
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• pp.160-167
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• 2017

Coating of individual cells with organic or inorganic materials has drawn a great deal of attention, because it provides the cells with physicochemical durability, which would contribute to the development of bioreactors, biosensor, and lab-on-a-chip, as well as to the fundamental studies in single cell-based biology. Although many strategies have been developed for coating of microbial cells, limited methods are available to coat mammalian cells because most mammalian cells do not have a robust membrane or exoskeleton. Instead, they are enclosed in a lipid bilayer, which is fluidic and vulnerable to changes in its environments. It is more difficult to treat mammalian cells in vitro than microbial cells because the surfaces of mammalian cells are not protected or reinforced by a tough coat. In this work, we report a cytocompatible and degradable nanocoat for mammalian cells. Three types of mammalian cells (HeLa cells, NIH 3T3 fibroblasts, and Jurkat T cells) were individually coated within metal-polyphenol. To maintain the viability of the mammalian cells, we performed the whole processes under strictly physiological culture conditions, and carefully selected nontoxic materials.

• IMMUNE NETWORK
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• 제2권4호
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• pp.183-188
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• 2002

Gradual attrition of telomere to a critical short length elicits successive cellular response of cellular senescence and crisis. Cancer cells evade this process by maintaining functional telomeres via one of two known mechanisms of telomere maintenance. The first and most frequent mechanism involves reactivation of enzyme activity of telomerase, a ribonucleoprotein complex mainly via transcriptional up-regulation of TERT, a catalytic subunit of telomerase complex. The second mechanism utilizes telomerase-independent way termed ALT (for Alternative Lengthening of Telomere), which possibly involves recombination pathways. Thus master key for cellular immortalization is supposed to possess adequate telomere reserves. Indeed, telomerase can alone induce the immortalization under culture on feeder cell layers without generally known inactivation mechanism of tumor suppressor genes. Including this phenomena, this review will focus on telomerase and telomere-associated proteins, thereby implication of these proteins for cellular immortalization processes.

• Animal cells and systems
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• 제6권1호
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• pp.59-64
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• 2002

To restore the forest ecosystem severely damaged by air pollution around industrial complexes, plants tolerant to the polluted environment were selected by transplant and pot culture experiments. A restoration plan by arranging those tolerant species was prepared based on the ecological diagnostic results on an area that requires restoration. Transplant experiment in Ulsan and Yeocheon areas, the representative industrial complexes in Korea, selected eight tolerant species of Quercus aliena, Q. acutissima, Q. dentata, Q. mongolica, Q. serrata, Ligustrum japonicum, Styrax japonica, and Poncirus trifoliata. Cultivation in the polluted soil transported from the Ulsan and Yeocheon industrial complexes chose five tolerant plants of Q. aliena, Q. acutissima, Q. serrata, Styrax japonica, and Alnus firma. A plan to restore the forest ecosystem of Mt. Dotjil, which experienced the severest ecosystem degradation in the Ulsan industrial complex, was prepared by applying those tolerant species along with treatment for soil amelioration. Arrangement of the tolerant species was designed by considering their ecological characteristics including distribution range on topography and shade tolerance. Soil amelioration was focused on the improvement of fertility and moisture conditions.

• Ecology and Resilient Infrastructure
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• 제7권3호
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• pp.199-207
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• 2020

전 세계적으로 홍수, 가뭄, 열파 등 기상재해가 빈발하는 환경에서 도시공원은 휴식, 문화, 생태적 기능뿐만 아니라 도시민의 안전을 위한 기능도 제공해야 한다. 본 연구는 도시 내 기후변화에 대응하는 녹색 공간인 생활권 공원에 복합기능을 확보하는 목적으로 도시인의 안전을 위한 도시방재공원의 분류체계를 제안하였다. 문헌 조사를 통해 분석지표를 추출하고, 대상지 현장조사 및 관련자 인터뷰를 통해 분류체계를 검증하였다. 평가를 위한 대분류는 도시공원의 입지, 공간구성, 방재복합시설 3가지로 구분하고, 실증 분석을 통하여 도출된 문제점에 대한 개선방향을 제안하였다.

• Mycobiology
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• 제29권1호
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• pp.1-6
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• 2001

Nine species of genus Ganoderma collected in Korea and abroad including Ganoderma lucidum complex and G. lucidum were compared by investigating growth characteristics. In the bottle culture, the mycelial growth periods of G. lucidum from Taiwan and North America was 26 to 30 days compared to that of Korean G. lucidum, which was 30 to 32 days. Cultivation period of Taiwan and North American isolates was 30 to 32 days which were 11 to 17 days shorter than those of Korean isolates. Biological efficiency of Taiwan and North American isolates were ranged from 3.3 to 5.5%, which were apparently lower than that of Korean isolates which ranged from 6.2 to 9.4%. Korean isolates had longer stipes($15{\sim}40$ mm) and more number of pileus($4{\sim}6$/bottle) than those of Taiwan and North American isolates. The G. lucidum isolates collected from Korea will be regarded as the independent species from the G. lucidum collected from Taiwan and North America since, the G. lucidum from Korea showed much different growth characteristics in various aspects compared to the G. lucidum from Taiwan and North America.

• 한국실내디자인학회논문집
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• 제22권5호
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• pp.162-170
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• 2013

Although vision has been considered as a top sensory organ in the Western culture until modern times, the significance of space experience that communicates with a body via complex perceptions has recently garnered attention. This study criticizes the limitations of a vision-centered space and has the goal of empirically examining the sensibility characteristics in a haptic space through post-structuralism views leading up to Gilles Deleuze's philosophical ideas. This study will explore the flow of senses from the philosophical and artistic standpoint and the sensibility characteristics in a haptic space by examining the theories on haptic perceptions in connection with a space. In order to test the existing theories and identify the differences in sensibilities depending on perceptual method, the sensibilities of participants were compared using SD method (Semantic Differential scale method) and a validity test was conducted using a statistical program SPSS. The research has revealed that the average sensitivity scale of complex haptic perception was higher than single perception that only relies on vision. In addition, there was a correlation between perceptual method based on various sensory organs and human sensitivities. This study is meaningful in that it can serve a foundation for empirical research on sensibility, since it has reexamined the relationship between space and participants and demonstrated and analyzed their sensitivities through an experiment.

• 한국농공학회논문집
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• 제52권3호
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• pp.47-55
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• 2010

Investigations on the inundation damage and improvement measures were carried out centering around the protected horticultural complex concentrated in lowlands on the side of Geum river, in Nonsan and Buyeo, Chungnam. Most greenhouses were single-span plastic houses in this area, and tomato, strawberry and watermelon were cultivated mainly. 45.8 % of whole farmhouse were experienced in damage by inundation, and a frequency of the damage was average once in 11 years. The most urgent problem at the greenhouse culture in this area was showed in order of drainage improvement, irrigation water resources and energy saving. Consideration items in drainage improvement project for protected horticulture were showed in order of extending drain pumps, extending drain canals, using concrete flume in drain ditch. It needs to consider systematic plans that can restrain new establishment of greenhouses on the lowland paddy field in drainage area. It is difficult to remove greenhouses which are already established or prohibit cultivation. Therefore we should impose minimum duty items so that greenhouse tillers can cope with inundation. And it is thought that managing agency need to minimize farmers damage by improving drainage ability and introducing maintenance pattern that is different from rice cropping.

• Archives of Pharmacal Research
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• 제14권4호
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• pp.325-329
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• 1991

Effects of Lycii Fructus on primary cultured chicken embryonic brain cells were studied by microscopic observation, determination of the activity of pyruvate dehydrogenase complex (PDHC), and syntheses of protein, RNA and DNA. The brain cells were prepared from the brains or 10-day-old chicken embryos and cultured with a deficient medium. The activity of PDHC in the brain cells cultured with a deficient medium was increased to 1.8 times by the addition of $30\;{\mu}g/ml$ of the total methanol extract of Lycii Fructus. To seek the active fraction, total methanol extract was further fractionated by the polarity. The survival rate of neuronal cells was significantly increased by the addition of $100\;{\mu}g/ml$ of the buthanol or aqueous fraction. At this concentration, the significant increase of the syntheses of protein and RNA, but not of DNA, indicates that the fractions may act on the neuronal cells which are known to be non-dividing cells.

• 비교문화연구
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• 제19권
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• pp.101-126
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• 2010

Narcissism is a pathological phenomenon and narcissistic subject always needs to put itself on the top and has interest in nothing but its own determination. The protagonist of Adversary by ?mmanuel Carr?re does pay careful attention to what others are thinking of him while he does not distinguish difference between object of love and himself. So he can be allegedly narcissistic subject. And it can be said that the behaviors of Mary in the movie Under the sand by Ozon are included in narcissism in that narcissistic subject has the characteristics that idealizes the object of libido. However, in this study, the heroine is examined based on such a point that the object of love and extreme idealization incline toward others. We call this case reverse-narcissism. In Part 2, it is investigated what relation narcissism as an unconscious psychological tool has with Oedipus complex, which plays an important role in forming human psyche. For instance, disappointment caused by prohibition at the oedipal stage is too severe, which creates superego and its idealization to protect in such a way that narcissistic regression can not be done. Cases of extremely big gap between ego and ideal type are perversion, impostor, mania, paranoia, etc, where narcissistic and oedipal elements are combined to affect.

• 비교문화연구
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• 제35권
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• pp.293-314
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• 2014

This paper aims to contrast phonology of Korean and Thai in terms of language universality. Considering consonants, both languages having 21 typologically most plausible consonants display high universality in the number of consonants. However, Thai shows higher universality in regards to their substance, i.e. it differs from Korean when it comes to the structure of plosives and fricatives. Both Korean and Thai show similarities regarding the plosives due to the fact that both languages possess three contrastive consonants. However, the Thai plosives consist of plain voiced, plain voiceless and aspirated voiceless sounds that have higher universality than the Korean plosives which are plain voiced, plain voiceless and aspirated voiceless. In case of vowels, both Korean with its 10 vowels and Thai with its 9 vowels show lower universality when it comes to the total number of vowels. However, all of those vowels belong to the list of most plausible vowels which makes their universality higher in substance. In respect of syllable structure, Korean with its CVC type shows a moderately complex structure while Thai with its CCVC type has a complex structure. The coda may consist of only one consonant in each language but onset is composed of one consonant in Korean, and two consonants in Thai. The contrastive study of similarities and differences between Korean and Thai in terms of phonology will help not only understand the two languages but also provide useful information for increasing the efficacy of Korean language education for Thai learners of Korean whose number is rapidly increasing.