• Title/Summary/Keyword: Culture Complex

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In Vitro Antagonistic Activity Evaluation of Lactic Acid Bacteria (LAB) Combined with Cellulase Enzyme Against Campylobacter jejuni Growth in Co-Culture

  • Dubois-Dauphin, Robin;Sabrina, Vandeplas;Isabelle, Didderen;Christopher, Marcq;Andre, Thewis;Philippe, Thonart
    • Journal of Microbiology and Biotechnology
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    • v.21 no.1
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    • pp.62-70
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    • 2011
  • The antibacterial effects of nine lactic acid bacteria (LAB) against Campylobacter jejuni were investigated by using agar gel diffusion and co-culture assays. Some differences were recorded between the inhibition effects measured with these two methods. Only two LAB, Lb. pentosus CWBI B78 and E. faecium THT, exhibited a clear anti- Campylobacter activity in co-culture assay with dehydrated poultry excreta mixed with ground straw (DPE/GS) as the only growth substrate source. It was observed that the supplementation of such medium with a cellulase A complex (Beldem S.A.) enhanced the antimicrobial effect of both LAB strains. The co-culture medium acidification and the C. jejuni were positively correlated with the cellulase A concentration. The antibacterial effect was characterized by the lactic acid production from the homofermentative E. faecium THT and the lactic and acetic acids production from the heterofermentative Lb. pentosus CWBI B78. The antagonistic properties of LAB strains and enzyme combination could be used in strategies aiming at the reduction of Campylobacter prevalence in the poultry production chain and consequently the risk of human infection.

A Study on the Clothing Culture for New Generation of Korea and Japan (2002 한.일 국민교류년 기념' 한국과 일본의 신세대의 복식업화에 대한 연구 - Street Fashion을 중심으로 -)

  • 백샘이;간호섭
    • Journal of the Korean Society of Costume
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    • v.53 no.3
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    • pp.31-49
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    • 2003
  • The Purpose of this research is to examine and contrast the cultural characteristics of the street fashion in new generations of Korea and Japan. We also intended to help clothing companies understand the current trend in this new generation. As the method for street fashion analysis. various literature, internet information and new media materials were utilized. Key observations obtained are as following First, they pursue a complex image. New generations seem to admix images from different areas to create a synthetic image, rather than adopting a single image; Second, practicality is an important factor. They Pursue a sense of their own unique trend: Third, distinction. The difference in society values between Koreans and Japanese is incorporated. The Korean new generation considers the traditional moral principles as important social values. In contrast the Japanese new generation is greatly influenced by the open sexuality culture ; Fourth. public ownership and sharing of the culture. There is a gradual deviation from a dominating unidirectional influence from one country on another. Now the two countries are influencing each other ; Fifth, sense. There are numerous fashion items and generalized total coordination. New generations express more active and creative fashion sense in those fashion items.

Optimization of Culture Conditions for Production of Pneumococcal Capsular Polysaccharide Type IV

  • Kim, S.N.;Min, K.K.;Choi, I.H.;Kim, S.W.;Pyo, S.N.;Rhee, D.K.
    • Archives of Pharmacal Research
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    • v.19 no.3
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    • pp.173-177
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    • 1996
  • The Pneumococcus, Streptococcus pneumoniae, has an ample polysaccharide (PS) capsule that is highly antigenic and is the main virulence factor of the organism. The capsular PS is the source of PS vaccine. This investigation was undertaken to optimize the culture conditions for the production of capsular PS by type 4 pneumococcus. Among several culture media, brain heart infusion (BHI) and Casitone based medium were found to support luxuriant growth of pneumococcus type 4 at the same level. Therefore in this study, the Casitone based medium was used to study optimization of the culture condition because of BHI broth's high cost and complex nature. The phase of growth which accomodated maximum PS production was exponential phase. Concentrations of glucose greater than 0.8% did not enhance growth or PS production. Substitution of nitrogen sources with other resources or supplementation of various concentrations of metal ion (with the exception of calcium, copper, and magnesium ions) had adverse effects on growth and PS production. On the other hand, low level aeration and supplementation of 3 mg/l concentration of asparagine, phenylalanine, or threonine were beneficial for increased PS production. The synergistic effect of all the favorable conditions observed in pneumococcal growth assays provided a two-fold cumulative increase in capsular PS production.

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The influence of traditional values on the development of fashion in Bali

  • Arumsari, Arini;Sachari, Agus;Kusmara, Andryanto Rikrik
    • The Research Journal of the Costume Culture
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    • v.27 no.3
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    • pp.264-273
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    • 2019
  • Fashion as a field of cultural studies is a very complex field that is very interesting to discuss, especially in Indonesia as the very rich country in cultures and past traditions. This research will discuss the development of fashion in Bali, one area that is opulent in its cultures. Based on the research conducted by today's fashion development, Bali is still strongly influenced by factors inherited from traditional values that exist in the Balinese culture. This research is conducted by applying the qualitative method with the ethnography approach to unravel any factor in Bali so that it becomes the foundation and triggers the rapid development of fashion by still maintaining the values of the tradition. Considering that the values of tradition as a whole have a good impact on the preservation of the natural balance and various factors that exist in society, whether these factors still exist in other regions of Indonesia and they are possible to be developed in various regions of Indonesia. So hopefully the development of fashion in Indonesia as a whole can maintain the local content that characterizes the Indonesian Nation and have a positive impact on the balance of nature and also to the community in Indonesia and the world culture.

Design of an Actuator Using Electro-active Polymer (EAP) Actuator with Composite Electrodes (복합재료 전극을 가진 전기활성고분자 구동기의 설계)

  • Kim, Dong-Uk;Chang, Seung-Hwan
    • Composites Research
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    • v.32 no.5
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    • pp.211-215
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    • 2019
  • The cell culture process under in vitro condition is much different from the actual human body environment. Therefore, in order to precisely simulate the human body environment, a dynamic cell culture device capable of delivering mechanical stimulation to cells is essential. However, conventional dynamic cell culture devices require relatively complicated devices such as tubes, pumps, and motors, and the mechanical stimuli delivered is also simple. In this study, an electro-active polymer actuator as a driving component is introduced to design simply driven dynamic cell culture device without complicated components. The device is capable of delivering relatively complex mechanical stimuli to the cells.

A Study on the Expressive Characteristics in Multi Functional Corporate Culture Space of experiential marketing (체험마케팅에 의한 기업의 복합문화 공간 표현특성에 관한 연구)

  • Park, So-Young;Yoon, Sang-Young
    • Korean Institute of Interior Design Journal
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    • v.21 no.3
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    • pp.31-39
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    • 2012
  • This is the time we need differentiated strategy due to consumer's standard of purchasing is becoming more complicated and corporate marketing war is intensely competitive. For a long-term strategy, making competitive corporate image is important because companies have their own competitiveness. In addition, importance of having a relationship with customer is magnified through consumers'direct and indirect experience. "The corporate complex cultural space is designed place for experiential marketing to convey its meaning to consumers." Under above concept, this study analyzed the theory of experiential marketing and conducted a survey which is based on experiential marketing strategic module (SEMs) using the analyzed theory. This study drew expression characteristics of space through case analysis and conducted a survey to have more effective corporate complex cultural space. As a result, the experience in the space enables people to understand of the space through five senses, not consist of simply chucks of sense-date, and this result emphasizes the importance of 'Event' because people tend to escape from restriction for reality to relive stress and have emotional satisfaction. In addition, it shows expression characteristics which provide sympathy as a flexible interaction through symbolical image. The result of the expression characteristics' survey is showed up to improve effect of corporate complex cultural space. This study is analyzing the corporate complex cultural space through experience marketing for the company which wants provide differentiated experience of various space expression methods and catching current position. Also, this study places its significance to give a way to increase space preference and effect of corporate complex cultural space. Based on the result of this study, I hope the future corporate complex cultural space will develop refreshingly.

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Individual, social, and cultural approaches to knowledge sharing

  • Widen, Gunilla
    • Journal of Information Science Theory and Practice
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    • v.5 no.3
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    • pp.6-14
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    • 2017
  • Workplace knowledge sharing is a complex process and there are a large number of studies in the area. In this article three theoretical approaches in library and information science are used to discuss knowledge sharing in the workplace. The approaches are information behavior, social capital, and information culture, and they bring important insights that need to be considered from a holistic management point of view when it comes to knowledge sharing. The individual's relation to different levels of context is important, meaning both in relation to work roles, work tasks, situations, organizational structures, and culture. The frameworks also shed light on where and how knowledge sharing activities are present in the organization. From a knowledge management point of view, it is important to acknowledge that when knowledge is valued, there is also an awareness of the knowledge sharing activities. Also, in addition to more traditional views of context, the frameworks bring forward different views on context, such as time and space as contextual factors.

Characterization of Benzoate Degradation via ortho-Cleavage by Streptomyces setonii

  • An, Hae-Reun;Park, Hyun-Joo;Kim, Eung-Soo
    • Journal of Microbiology and Biotechnology
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    • v.10 no.1
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    • pp.111-114
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    • 2000
  • Streptomyces are widespread in nature and play a very important role in the biosynthesis as well as biodegradation of natural and unnatural aromatic compounds. Both qualitatively and quantitatively through TLC and UV spectrophotometric assays, it was observed that the thermophilic soil bacteria S. setonii (ATCC 39116), which can utilize a benzoate as a sole carbon and energy source in a minimal liquid culture, was not very sensitive to the benzoate concentation and to the culture conditions such as the pH and temperature. The in vitro conversion of a catechol to a cis, cis-muconic acid by a crude S. setonii lysate implies that the aromatic ring cleavage by S. setonii is initiated by a thermostable catechol-1,2-dioxygenase, the key enzyme in the ortho-cleavage pathway of aromatic compound biodegradation. Unlike non-degrading S. lividans, S.setonii was also highly resistant to other similar hazardous aromatic compounds, exhibiting almost no adverse effect on its growth in a complex liquid culture.

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Comparative Study on Graphic Examples of Traditional Lattices in Korea, China, Japan - Focused on grid type from geometrical viewpoint - (한국, 중국, 일본의 전통 창살 그래픽 예제 비교 연구 - 기하학적 관점에서 그리드 형태를 중심으로 -)

  • 박지연;이연숙
    • Korean Institute of Interior Design Journal
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    • no.23
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    • pp.139-147
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    • 2000
  • The cultural identity of design is very critical in the modern society which is called the society of complex culture and multi-culture. In addition, adopting a traditional lattice-like pattern as a composition element of interior space is an effective way in the context of adding a traditional touch to the modern interior space. Meanwhile, in the process of applying Korean traditional lattices to modern interior space, some problems occurred: it has not been properly embodied; or it has been mistaken for those of China and Japan sharing similar culture with Korea. Thus, this study is designed to have precise knowledge of Korea, Chinese and Japanese traditional lattices. Besides, typical patterns of each nation's lattices are comparatively analyzed from the geometrical viewpoint, in a bid to pave the way for th modernization of traditional lattices. In particular, standards are set and proposed to identify different nations's lattices mainly focusing on grid type lattices which are often used and confusing.

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"Once You Go Black": Performative Acts of "Blackness" in Contemporary Cinema

  • Chung, Hye Jean
    • English & American cultural studies
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    • v.14 no.1
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    • pp.241-267
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    • 2014
  • Media representations of race have attempted to contain blackness by packaging and commodifying it to reflect and affect preconceptions and prejudices of dominant culture. From the early beginnings of blackface minstrelsy as entertainment form in the $19^{th}$ century, representations of African Americans in popular culture and mainstream media have been closely associated with the notion of performance. The performative nature of racial representations is situated within the discursive struggle over what it meant to be Black, or what it meant to be labeled and portrayed as Black in American culture. This essay discusses four films that contain performances of "blackness" that assemble race and gender in complex configurations: Bamboozled (Spike Lee, 2000), Girl 6 (Spike Lee, 1996), Big Momma's House (Raja Gosnell, 2000), and White Chicks (Keenen Ivory Wayans, 2004). I explore how the performative nature of "blackness" is emphasized, thematized, and problematized in these films through the physicality of corporeal figures that embody the close link between race and gender identities. Once we are cognizant of the fact that race and gender are fabricated cultural constructs and performative acts, we can recognize that notions of "blackness" and "femininity" are not naturalized or essentialist, but open to recontextualization and revision.