• 제목/요약/키워드: Culture Collection

검색결과 880건 처리시간 0.024초

Study on the In Vitro Maturation and Sperm Penetration Rates of Canine Oocytes

  • Park, Ji-Hoon;Seok, Ho-Bong;Kim, Sang-Keun
    • Reproductive and Developmental Biology
    • /
    • 제34권1호
    • /
    • pp.21-25
    • /
    • 2010
  • The purpose of this study was to investigate the effects of the collection time, co-culture and sperm penetration of canine oocytes on in vitro maturation and fertilization. The oocytes were cultured in TCM-199 media containing hormonal supplements (10% FCS, 10 IU/ml HCG, 10 IU/ml PMSG) at 5% $CO_2$, 95% air, $38^{\circ}C$. The in vitro maturation rate to MII stage of in vitro oocytes recovered from ovaries that collected at follicular, luteal and inactive phases of the reproductive phase for 44~72 hrs were 19.2%, 12.2%, and 6.0%, respectively. Follicular phases oocytes had a significantly higher in vitro maturation rate than oocytes collected at luteal and anestrus stage (p<0.05). The in vitro maturation rates to the MII stage of canine oocytes after 48 hrs of culture with glutathione, pyruvate, or glutathione + pyruvate were 12.5%, 10.7%, and 17.5%, respectively. This was higher than that in both alone or the combination of the two compared to the control group (19.0%). The sperm penetration rates of in vitro matured oocytes by fresh and frozen semen were 29/80 (36.3%) and 18/80 (22.5%), respectively. Although there are limited reports about canine oocytes co-culture and in vitro fertilization, our results on in vitro maturation is comparable to the results from other researches.

마린 세르 패션 컬렉션에 나타난 지속가능 디자인 특성 (Characteristics of sustainable fashion design in Marine Serre collection)

  • 이수현;이연희
    • 복식문화연구
    • /
    • 제32권1호
    • /
    • pp.108-123
    • /
    • 2024
  • This study aimed to explore sustainable fashion design plans and directions by analyzing Marine Serre's collection. Previous research was reviewed to derive classifications of the aesthetic characteristics of sustainable fashion design. This classification was then used to analyze the characteristics of the Marine Serre collection. Design analysis was conducted on Marine Serre's 2018 FW to 2023 SS collections. Marine Serre's sustainability characteristics are functionality, surprise, handicraft, and inclusion. The results are as follows. First, functionality is the highest among the four characteristics and includes the functionality of movement, the functionality of form, and futurism. This characteristic was observed in the use of all-in-one body suits, pockets, and workwear, showing the will and values of designers who value daily activity. Second, surprise includes the scarcity of materials and the unexpectedness of composition. The value of the clothing is enhanced by the use of scarce materials not typically used in clothing. In addition, Marine Serre is highly regarded for expanding clothing into life by incorporating material upcycling into the theme of the collection. Third, handcrafted features include exaggerated decorations, logo, retro designs, and natural properties, and intentional utilization is differentiated. Marine Serre's signature pattern suggests a suitable expression for the fabric to use the crescent moon for the season. Fourth, the collection expresses themes of inclusivity and cultural diversity. The results indicate that Marine Serre wants to contribute to a better future characterized by global coexistence.

Conceptual Clothing Design Process Using Cooperative Learning Strategies: Senior Clothing Design Class

  • Sohn, MyungHee;Kim, Dong-Eun
    • Fashion, Industry and Education
    • /
    • 제14권1호
    • /
    • pp.59-68
    • /
    • 2016
  • This paper identified the source of inspiration to cooperatively design a fashion collection from US undergraduate clothing design students and addressed how to implement team-based learning strategy to conceptual clothing design in class. Data was collected from the total of 51 students in a senior clothing design course at a large 4-year university in the US. The assigned project for this class was to develop a group collection under a same theme. Each student worked with his/her team member(s) to create an outfit and the entire class worked as a group to create a cohesive collection. The study showed that the sources of inspiration for the themes/concepts came from 11categories: historic era/old Hollywood glamour, shape/line/structure/architectural, fairy tales movies, nature/abstract, circus/mysterious, occasion/place, object, designer/artist, futuristic, culture, and various movies. To implement cooperative learning strategies in the clothing design class, a total of five class presentation/discussion sessions were held for theme/concept decision, fabric decision, design decision, test garment evaluation and design modification, and final products. Throughout the design process, team-based learning strategy promoted students' engagement and participation and inspired their critical thinking skills for making decisions within a team.

An Analysis of Deng Sanmu's Seal Cutting Emulation-Centering on Ancient Seal, Qin Seal and Han Seal

  • Li, Zhuying
    • International Journal of Advanced Culture Technology
    • /
    • 제10권4호
    • /
    • pp.219-225
    • /
    • 2022
  • In November 1983, Deng Sanmu's wife, Zhang Jianquan, donated a large number of Deng Sanmu's original seal carving stones and seal cutting scores to the Heilongjiang Provincial Museum in China. Therefore, the Heilongjiang Provincial Museum of China is the richest and most authoritative place to collect Deng Sanmu's original seal cutting stones and seal carving scores. According to the fieldwork, the original seal cutting stones and the seal cutting score donated by Deng Sanmu's wife, Zhang Jianquan, include Deng Sanmu's seal cutting inscriptions made between 1922 and 1963. In addition, Deng Sanmu's own life and career in the art are documented in detail in his markings, so the seal cutting inscriptions of Deng Sanmu in the collection of the Heilongjiang Provincial Museum of China are quite representative. We paper is a documentary review and study of the seal inscriptions of Deng Sanmu in the collection of the Heilongjiang Provincial Museum in China. For the accuracy and richness of this study, a total of 89 representative seals in the collection are used as primary sources to study Deng Sanmu's seal cutting emulation method. The specific content is centered on ancient seals, Qin seals, and Han seals, from which the lineage of Deng Sanmu's emulation methods of seal cutting is further clarified.

비비안 탐 컬렉션에 나타난 중국풍 디자인 특성 연구 (A study on Characteristics of Chinese Style Reflected in the Vivienne Tam Collection)

  • 강란영;박주희
    • 한국의류산업학회지
    • /
    • 제21권5호
    • /
    • pp.527-539
    • /
    • 2019
  • This study examined the Chinese style in the Vivienne Tam collection as well as explored diversity and modern interpretation of a Chinese style used in modern fashion. The study also investigated the concept and history of the Chinese style as well as analyzed the Chinese style in the collection based on a consideration of a Chinese element that is the basic structure with an understanding of Vivienne Tam and general collection themes. As a result of research, the Chinese style appeared in the Vivienne Tam collection was comprehensive with 43 Chinese elements including Animals, Flower & Grass among the Nature elements, Mythology Religion among the Figure elements, Historical Artifacts, Clothing, Oral Literature, Entertainment, Fine Arts. Technique, Folk holiday among Culture elements and Philosophy among Spirit elements, which accounted for 57.6% of the collection. The formative characteristics that included Chinese elements were extended, abstracted, distorted or transformed; in addition, some abstract elements were embodied into animals, images and certain particular forms to present a theme. In addition to two-dimensional method to print Chinese elements on fabric, they were also expressed as a three-dimensional texture or a silhouette, achieving clear and bold harmony. The aesthetic characteristic is a mixture of Eastern and Western way of thinking. Her costume has a political and historical meaning beyond the imitation of elements that represent the development of a pleasant and interesting design.

Isolation of Multidrug-Resistant Salmonella typhimurium DT104 from Swine in Korea

  • Lee, Ki-Eun;Lee, Yeon-Hee
    • Journal of Microbiology
    • /
    • 제45권6호
    • /
    • pp.590-592
    • /
    • 2007
  • We report the isolation of Salmonella enterica serotype Typhimurium phage type DT104 (CCARM 8104) from swine in Korea. The CCARM 8104 isolate was resistant to nalidixic acid and showed reduced susceptibility to quinolones. The CCARM 8104 isolate had a missense mutation, Asp87Asn, in the quinolone resistance-determining region in gyrA and produced PSE-1. The CCARM 8104 isolate carried two different class 1 integrons, and the PSE-1 ${\beta}$-lactamase gene was inserted into a 1,200 bp class 1 integron. The presence of DT104 with pse-1 in an integron located in a plasmid and reduced susceptibility to quinolone in swine pose a significant threat of possible horizontal spread between swine and humans.

Analysis of Vaginal Lactic Acid Producing Bacteria in Healthy Women

  • Nam, Hye-Ran;Whang, Kyung-Hee;Lee, Yeon-Hee
    • Journal of Microbiology
    • /
    • 제45권6호
    • /
    • pp.515-520
    • /
    • 2007
  • Vaginal lactic acid-producing bacteria of 80 pre-menopausal women were studied by isolation on Blood and DeMan-Rogosa-Sharpe agar, PCR with group-specific primers for Lactobacillus-denaturing gradient gel electrophoresis (DGGE), and PCR with specific primers for V3 region in 16S rRNA-temporal temperature gel electrophoresis (TTGE). Conventional isolation method on media detected only one lactobacillus (Lactobacillus brevis) while TTGE detected only Lactobacillus sp. DGGE detected seven Lactobacillus species; L. coleohominis, L. crispatus, L. iners, L. reuteri, L. rhamnosus, L. vaginalis, and Leuconostoc lactis. L. acidophilus and L. gasseri, which are prevalent in Western women, were not detected in Korean women. Furthermore, L. rhamnosus, Leuc. lactis, L. coleohominis, and Weissella cibaria, which were not previously reported in the vaginal microbiota of Korean women, were detected. The five most prevalent LABs in vaginal microbiota in Korean women were L. iners, Enterococcus faecalis, L. crispatus, Leuc. lactis, and W. cibaria.

크리스티앙 디오르의 디자인 경향 분석 - $2003{\sim}2005$년 컬렉션을 중심으로 - (Analysis on Design Trend of Christian Dior - Focused on from 2003 to 2005 Collection -)

  • 조진숙;정하경
    • 복식문화연구
    • /
    • 제15권5호
    • /
    • pp.825-837
    • /
    • 2007
  • To analyze the design of Christian Dior, 216 pieces of Dior's works were collected and studied from the COLLECTION(Seoul: Dong Ah TV) from 2003 to 2005. The result is as follows. In recent three year period, Christian Dior maintained the feminine image and implemented different additions such as sexy, ethnic, avant-garde and casual image in every season. Also, to reflect a new trend or fashion sense, images of ethnic, retro, hippie or vintage were applied. In using color, different main color(YR, R, Y, BG, RP, B) were chosen every season to pursue variations. For the pattern usage, feminine beauty was presented by numerous applications of flowered pattern. Also the practice of pattern and hue in different rations in every season pursued variations in design.

  • PDF

Production of c9,t11- and t10,c12-conjugated Linoleic Acids in Humans by Lactobacillus rhamnosus PL60

  • Lee, Ki-Eun;Lee, Yeon-Hee
    • Journal of Microbiology and Biotechnology
    • /
    • 제19권12호
    • /
    • pp.1617-1619
    • /
    • 2009
  • Lactobacillus rhamnosus PL60 was tested for whether it can produce c9,t11- and t10,c12-conjugated linoleic acids (CLAs) in human. After consumption of L. rhamnosus PL60, L. rhamnosus was detected in feces 1 week after the start of intake. Analysis by gas chromatography showed that concentrations of c9,t11- and t10,c12-CLAs in serum had increased and concentrations of serum leptin had significantly decreased. Results showed that L. rhamnosus PL60 can survive in human intestines and produce CLAs in humans. This is the first report that bacteria can produce CLAs in humans.