• 농업과학연구
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• 제47권1호
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• pp.139-161
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• 2020

The aims of this study were to classify the cultivation status of medicinal crop farmers and to obtain basic data on domestic medicinal crops. To this end, 406 medicinal crop farmers participating in farming education programs in 14 cities and counties were surveyed over 10 months. The results were as follows. First, the biggest difficulty farmers faced in the overall production of medicinal crops was a lack of cultivation skills. Second, in a detailed inquiry about each difficulty item, the most difficult problems were injury by continuous cropping of root-using crops, shortage of specific farm machinery, uncertain market prices, and lack of knowledge about pest control or the positive list system (PLS). Third, the profitability of medicinal crops increased with career experience. Among the farmers who earned profits, requests for education and technical guidance were the most important factors for profitability. Fourth, the results of the analysis suggest that to achieve stable cultivation of medicinal crops, the following items need to be addressed: development and supply of basic cultivation technologies, climate response, registration of pest control pesticides, a solution to the injury by continuous cropping, and contracting cultivation expansion. Additionally, to improve profitability, it is necessary to create conditions that enable the same crops to grow for a long time in one region.

• 한국버섯학회지
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• 제15권1호
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• pp.14-20
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• 2017

버섯의 단위면적당 생산량을 증대시키기 위하여 다단으로 재배되는 버섯재배사는 재배균상이 내부공기의 유동을 막는 장애물로 작용하여 균상의 아래쪽과 위쪽간의 환경차이가 발생한다. 이러한 환경차이 때문에 버섯재배 균상의 위치에 따른 생육차이가 발생한다. 본 연구에서와 같이 버섯재배사 내부에서 버섯이 생육되지 않는 작업통로의 상단에 천장방향으로 대류팬을 설치하여 운용하면 재배사 내부의 공기를 전체적으로 균일하게 유지할 수 있는 것으로 나타났다. 다만, 버섯재배사 내부에 설치된 각종 환경조절장치 즉 유니트쿨러, 입기팬, 배기팬, 대류팬, 가습기 등이 모두 한꺼번에 가동될 때는 버섯재배 균상위의 RMS 값이 속도 23.86%, 온도 6.08%, 습도 2.72%로 나타났다. 하지만 유니트쿨러와 대류팬이 가동될 때는 균상위의 RMS 값이 속도 23.54%, 온도 0.51%, 습도 0.41%로 나타났다. 따라서 버섯재배사의 환경조절을 위한 입기팬, 배기팬과 가습기는 용량이 큰 것을 설치하여 전체적인 가동시간을 줄이고, 유니트쿨러와 대류팬을 조합한 환경관리 방법이 내부 환경의 균일성 향상에 큰 도움이 될 것으로 판단된다.

• 한국공간정보시스템학회 논문지
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• 제10권3호
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• pp.93-106
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• 2008

농업은 기상 의존도가 매우 높은 산업분야로서 지구온난화는 농업의 생산성, 생산여건, 그리고 품질 및 재배적지 등에 미칠 영향이 매우 클 것이 예상된다. 따라서 본 연구에서는 지리정보시스템을 도입하여 지구 온난화에 따른 국내 과수작물 재배지의 입지분석을 기후변화에 따라 우리나라에 확대도입이 가능한 레몬, 무화과, 키위, 오렌지, 석류, 한라봉을 중심으로 시행하였다. 지리정보시스템의 기술적 측면에서 기온에 대한 Interpolate 기능과 경사도에 대한 surface analysis 기능, 그리고 raster Calculator를 이용하였다. 기온의 상승에 따라 각 과수들은 재배지역이 현재는 제주도와 남부지역에 집중되어 있으나, 평균기온이나 최저기온이 3도, 4.5도 상승함에 따라 두 가지의 형태 즉, 중부지역으로 확산되거나 띠모양으로 변화하는 모습을 보이고 있다. 이러한 연구결과를 통해서 우리는 지구온난화라는 위기를 기회로 활용하고 더 나아가 농촌의 농가 소득향상과 국민에게 다양한 먹을거리를 제공하는 차원에서 기후 자원을 활용할 수 있는 정부의 정책적 노력과 농민들의 합리적인 대응이 시급하게 필요함을 주장하지 않을 수 없다.

• 한국자원식물학회지
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• 제33권6호
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• pp.651-658
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• 2020

The aim of this study was to investigate the comparative growth characteristics and ginenoside contents of wild-simulated ginseng on different years (7 and 13-year-old) by monitoring soil properties of cultivation regions. Plant and soil samples were collected from 6 different cultivation regions. Soil organic matter (OM), total nitrogen (TN) and cation exchangeable capacity (CEC) were significantly higher in 13-year-old wild-simulated ginseng cultivation regions compared to 7-year-old wild-simulated ginseng cultivation regions. Growth characteristics of wild-simulated ginseng had shown significantly higher in 13-year-old wild-simulated ginseng compared to 7-year-old wild-simulated ginseng. Ginsenoside G-Rb1, Rb2, Rc, Rd, Re, Rf, Rg1 were significantly higher in 13-year-old wild-simulated ginseng than 7-year-old wild-simulated ginseng. According to the results of correlation analysis, soil OM, TN and CEC of the cultivated regions were positively correlated with the growth of wild-simulated ginseng. In addition, the root length of wild-simulated ginseng showed positive correlation with ginsenoside content. Hence, this study was able to investigate the correlation between growth and ginsenoside content of wild-simulated ginseng based on soil characteristics of the cultivation regions.

• BMB Reports
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• 제40권1호
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• pp.22-28
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• 2007

MyoD, expressed in skeletal muscle lineages of vertebrate embryo, is one of muscle-specific basic helix-loop-helix (bHLH) transcription factors, which plays a key role in the determination and differentiation of all skeletal muscle lineages. In this study, a cDNA of grass carp MyoD was cloned and characterized from total RNA of grass carp embryos by RT-PCR. The full-length cDNA of grass carp MyoD is 1597 bp. The cDNA sequence analysis reveals an open reading frame of 825 bp coding for a protein of 275 amino acids, which includes a bHLH domain composed of basic domain (1-84th amino acids) and HLH domain (98-142th amino acids), without signal peptide. Then the MyoD cDNA of grass carp was cloned to yeast expression vector pPICZ$\alpha$A and transformed into P. pastoris GS115 strain, the recombinant MyoD protein with a molecular weight of about 31KD was obtained after inducing for 2d with 0.5% methanol in pH 8.0 BMGY medium, and the maximum yield was about 250 mg/L in shaking-flask fermentation. The results were expected to benefit for further studies on the crystal structure and physiological function of fish MyoD.

• 한국농공학회논문집
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• 제63권4호
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• pp.13-21
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• 2021

The feasibility of HWS for agricultural use was analyzed through a crop cultivation test to utilize the hydroponic waste solution (HWS) generated from the nutriculture greenhouse. The fertilizing effect of HWS was assessed on the basis of the inorganic nitrogen (N) mostly existed in HWSs, and nitrogen (urea) fertilizer. Lettuce was selected as the target crop influenced by the soil treatment and also for the crop cultivation test. Thus, the change in growth characteristics of lettuce and that in chemical characteristics of the soil were investigated. In terms of the growth of lettuce, the C control group with 70% nitrogen (urea) fertilizer and 30% HWS and the D control group with 50% nitrogen (urea) fertilizer and 50% HWS were more effective than the practice control group (B) with 100% nitrogen (urea) fertilizer. The results of this study confirmed the combined applicability of the chemical fertilizer and HWS for crop cultivation. Because NO₃-N present in HWS has a high possibility of leaching into the soil, its applicability as a fertilizer has been considered to be relatively low in Korea. However, if an appropriate mixing ratio of urea fertilizer and HWS could be applied, the problems associated with leaching of nitrate nitrogen could be reduced with beneficial effects on crop cultivation. Thus, future studies are required on the treatment effect of HWS with repeated cultivation, impact assessment on the surrounding environment, and appropriate fertilization methods using nitrogen (urea) fertilizer and HWS. These studies would facilitate the sustainable recycling of HWS.

• 인간식물환경학회지
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• 제23권3호
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• pp.277-291
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• 2020

Background and objective: The purpose of this study is to investigate the effects of visitors' positive emotions on satisfaction and loyalty with the perception of restorative environment of a healing garden created in an urban agriculture expo. Methods: The psychological indicators to the images of the healing garden were analyzed by the visitors' demographic variables and the three factors of plant cultivation activity level: plant cultivation experience, plant preference, and plant-related event. Results: Between age groups and occupational groups, significant differences were found statistically. The Perceived Restorativeness Scale(PRS) showed significantly differences between age groups in repose, fascination and legibility. The Positive Affect & Negative Affect Schedule(PANAS) showed statistically significant differences between age groups in positive emotions. In addition, we investigated the correlation between the PANAS and the three factors of plant cultivation experience level, the four factors of the PRS, satisfaction and loyalty. The three factors of plant cultivation experience level, the four factors of the PRS, satisfaction and loyalty showed a positive correlation with positive emotions and were inversely correlated with negative emotions significantly. Multiple regression analysis with dummy variables was conducted to examine the effects of plant cultivation activity level, attention restoration, and the PANAS on healing garden visitors' satisfaction and loyalty. As a result, among the four factors of the PRS, fascination and positive affectivity were significant variables that affect healing garden visitors' satisfaction and loyalty. Conclusion: The results indicated that the higher the attention restoration of visitors due to the fascination of the healing garden and the higher their positive affectivity and the more they have plant-related memories, the higher their impact on healing garden visitors' satisfaction and loyalty. Therefore, fascinating natural environments or greenery landscapes like healing gardens where people can contact plants would reduce negative emotions such as anger and sadness but to increase positive emotions such as pleasure, joy and satisfaction.

• 한국약용작물학회지
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• 제24권2호
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• pp.93-100
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• 2016

Background: A set of logical criteria that can accurately identify and verify the cultivation region of raw materials is a critical tool for the scientific management of traditional herbal medicine. Methods and Results: Volatile compounds were obtained from 19 and 32 samples of Angelica gigas Nakai cultivated in Korea and China, respectively, by using steam distillation extraction. The metabolites were identified using GC/MS by querying against the NIST reference library. Data binning was performed to normalize the number of variables used in statistical analysis. Multivariate statistical analyses, such as Principal Component Analysis (PCA), Partial Least Squares-Discriminant Analysis (PLS-DA), and Orthogonal Partial Least Squares-Discriminant Analysis (OPLS-DA) were performed using the SIMCA-P software. Significant variables with a Variable Importance in the Projection (VIP) score higher than 1.0 as obtained through OPLS-DA and those that resulted in p-values less than 0.05 through one-way ANOVA were selected to verify the marker compounds. Among the 19 variables extracted, styrene, ${\alpha}$-pinene, and ${\beta}$-terpinene were selected as markers to indicate the origin of A. gigas. Conclusions: The statistical model developed was suitable for determination of the geographical origin of A. gigas. The cultivation regions of six Korean and eight Chinese A. gigas. samples were predicted using the established OPLS-DA model and it was confirmed that 13 of the 14 samples were accurately classified.

• 한국토양비료학회지
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• 제45권2호
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• pp.280-286
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• 2012

본 연구는 바이오가스 생산시설과 연계하는 시설채소 오이의 청정에너지 농업 시스템 구축을 위하여 물질 및 에너지 수지 분석하였으며, 물질 및 에너지 수지 분석을 통해 시설채소 청정에너지 시스템의 도입 방안을 검토하였다. 시설 채소 오이 재배지의 연간 가온용 순에너지요구량 ($E_{YHED}$)을 충족시키는 바이오가스양은 촉성과 반촉성 재배에서 각각 9,482, $2,636Nm^3\;10a^{-1}$ (60% 메탄함량을 기준)이었으며, 바이오가스 생산을 위해서 각각 양돈슬러리 511, $142m^3\;10a^{-1}$가 요구되었다. 해당 양돈슬러리에서 발생하는 질소(N)와 인산 ($P_2O_5$)은 촉성재배에서 1,788, $511kg\;10a^{-1}$, 반촉성 재배에서 497, $142kg\;10a^{-1}$이었으며, 비료성분의 농지환원을 위해서는 촉성 재배의 경우 질소시비 기준 7.5 ha, 반촉성 재배의 경우 질소시비 기준 2.1 ha의 오이재배 면적이 요구되었다. 가온기간 중 촉성 재배에서 일일 가온에너지 요구량 ($E_{i,DHED}$)은 최소 7.7, 최대 515.1, 평균 $310.2Mcal\;10a^{-1}\;day^{-1}$을 나타냈으며, 반촉성 재배에서 일일 가온에너지 요구량 ($E_{i,DHED}$)은 최소 5.3, 최대 258.0, 평균 $165.1Mcal\;10a^{-1}\;day^{-1}$을 나타났다. 촉성 및 반촉성 재배에서 일일 가온에너지 요구량 ($E_{i,DHED}$)의 평균치를 기준으로 산출한 바이오가스 생산 시설의 양돈슬러리 유입용량은 각각 3.3, $1.7m^3\;day^{-1}$이었으며, 일일 가온에너지 요구량 ($E_{i,DHED}$)의 최대값을 기준으로 한 유입용량은 각각 5.4, $2.7m^3\;day^{-1}$로 나타났다. 또한 소화액의 처리측면에서 지역특성에 따라 액비이용을 고려한 바이오가스 생산시설 용량설계와 하절기의 잉여 바이오가스 활용 방안의 모색이 필요하였다.

• 한국작물학회지
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• 제62권2호
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• pp.149-155
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• 2017

본 연구는 야콘의 안정적 생산을 위한 재배지대 설정을 위해 2011년부터 2013년까지 4년간 8개 지역을 대상으로 생산성과 기능성 성분 함량을 조사하였고, 기상 등 재배환경과의 관련성을 검토하였다. 이들 데이터를 활용하여 주성분 분석을 수행한 결과는 다음과 같다. 1. 야콘 8재배지역에서 16개의 농업적 형질과 기상데이터을 조사한 결과 재배지대를 구분할 수 있는 기준은 p < 0.001 수준에서 평균온도, 최고온도, 최저온도, 무상일수 및 프락토올리고당으로 나타났으며, p < 0.01 수준에서 총수량, 상품수량, 글루코스 및 환원당으로 나타났다. 2. 야콘 재배지대별 주성분 분석에 이용된 16개의 농업적 형질 및 기상자료 중 제 1주성분은 약 8개, 제 2 및 3주성분은 각각 약 4개의 형질을 대표하고 있는 것으로 나타났으며, 특히, 야콘 재배지대를 분류하는데 있어서 생산성, 프락토올리고당, 기후변화와 관련된 요인이 중요한 기준이 되는 것으로 판단되었다. 3. 야콘 재배지대별 주성분 분석한 결과, 3개의 그룹으로 구분되었다. Group I에서는 상품수량이 2,622~3,196 kg/10a로 가장 많았고, 프락토올리고당 함량이 9.04~9.62%로 높았고, 평균온도가 $17.3{\sim}18.5^{\circ}C$로 서늘한 기후 조건을 나타내는 해발 500~560 m의 준고랭지 지역인 진부, 봉화 지역이었다. Group II에서는 상품수량이 가장 적었고, 프락토올리고당 함량이 비교적 적고, 최고온도가 가장 높은 해발 20~180 m의 평난지인 순천, 옥천, 여주, 강릉 지역이었다. Group III에서는 Group I과 Group II의 중간 값을 나타내었다. 4. 야콘 재배지역에 따라 기온환경에 따라 상품수량 및 프락토올리고당 함량이 차이를 나타내어 재배적지를 판단하는데 온도조건과 무상일수가 중요한 지표로 판단되므로 품질이 우수한 야콘을 생산하기 위해서는 준고랭지인 해발 500~560 m에서 재배하는 것이 수량과 기능성 물질인 프락토올리고당 함량이 높을 것으로 판단된다.