• Journal of the Korean Society for Marine Environment & Energy
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• v.9 no.3
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• pp.168-175
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• 2006

The cultural grounds of lave r have been surveyed using SPOT-5 satellite images. The facilities of laver cultivation area in the coastal waters of Korea were calculated. 10 m resolution multispectral images of SPOT-5 are adopted for the southern are a of Jebu Island, Hwaseong city to develop an automatic detection approach of laver nets that consists of the following: band difference technique, canny edge detector and morphological analysis: The number of satellite-based facilities was relatively high as compared with the licensed number in 2005, 676,749 chaek and 572,745 chaek(柵, unit of measure for laver farm), respectively. The ratio of a law abiding facility was very low at 52.9%. These data could be applied to control its national production keeping a stable market price for the government body.

• Journal of Biosystems Engineering
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• v.30 no.3 s.110
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• pp.185-191
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• 2005

Importance of alternative energy has been increasing due to environmental issues and lack of fossil fuels. In addition, heating cost that occupies from 30 to $40\%$ of the total production cost in the protected cultivation sector in Korea needs to be reduced for profitability and global competition. But, study on geothermal energy to solve these problems has not been activated for Korean protected cultivation. This study was conducted to develop an optimized geothermal exchange system through fundamental test of heat transfer characteristics in soil such as thermal diffusivity, changes in soil temperature during heating and cooling operations, and restorations of soil temperature after the heater was fumed off, These issues were investigated using computer simulation for different depths. The simulated characteristics were evaluated through controlled tests. Simulated characteristics of heat transfer in the soil at different depths showed a reasonable agreement with the results of the controlled tests. All of computer simulation and controlled tests, soil temperatures changed at 10cm and 20cm distance from pipe. but don't change at more than 30cm distance. It means that distances of heat transfer of the soil ranged from 20 to 30cm a day. Based on these results, the optimum spacing between adjacent heat exchange pipes and the pitch were selected as 50 and 40cm, respectively.

• Journal of Biosystems Engineering
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• v.40 no.1
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• pp.35-46
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• 2015

Purpose: To present a flexible and accurate autonomous solution for creating any desired row spacing value between the hydroponic gullies in multilayer growing units, and evaluate the capabilities and performance of the relevant automated system through the use of virtual prototyping technique. Methods: To build the virtual prototype of the system, CAD models of its different parts, including an autonomous vehicle and the mechanical mechanisms embedded in the multilayer growing unit, were developed and imported into the RecurDyn simulation software. In order to implement the automated row spacing operation, three spacing modes with different loading cycles and working steps were defined, and the operation of the system was simulated to obtain the target row spacing values specified for each of these modes. Results: Motion profiles related to the horizontal displacement of: 1) the lower and upper sliding bars installed in the cultivation layers, and 2) the hydroponic gullies, during the simulation of the system operation, were generated and analyzed. No deviation from the specified target spacing values was observed at the end of simulations for all spacing modes. Conclusions: The results of the motion analysis obtained by simulating the system operation confirm the effectiveness of the control scheme proposed for automated row spacing of gullies. It was also found that proper sequencing of the loading cycles and the precision of the working strokes of the upper bars are the critical factors for establishing a certain row spacing value. Based on the simulation results, precise control of the back and forth motions of the upper bars is highly necessary for sound operation of the real system.

• Journal of Korean Society of Rural Planning
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• v.19 no.4
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• pp.125-135
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• 2013

Analysis and evaluation of uncertainty is adopting the advanced methodology among the methods for greenhouse gas emission assessment that was defined in GPS2000 (Good practice guideline 2000) and GPG-LULUCF (GPG Land Use, Land-Use Change and Forestry). In 2006 IPCC guideline, two approaches are suggested to explain the uncertainty for each section with a national net emission and a prediction value on uncertainty as follows; 1) Spread sheet calculation based on the error propagation algorithm that was simplified with some assumptions, and 2) Monte carlo simulation that can be utilized in general purposes. There are few researches on the agricultural field including greenhouse gas emission that is generated from livestock and cultivation lands due to lack of information for statistic data, emission coefficient, and complicated emission formula. The main objective of this study is to suggest an evaluation method for the uncertainty of greenhouse gas emission in agricultural field by means of intercomparison of the prediction value on uncertainties which were estimated by spread sheet calculation and monte carlo simulation. A statistic analysis for probability density function for uncertainty of emission rate was carried out by targeting livestock intestinal fermentation, excrements treatment, and direct/indirect emission from agricultural lands and rice cultivation. It was suggested to minimize uncertainty by means of extraction of emission coefficient according to each targeting section.

• Korean Journal of Soil Science and Fertilizer
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• v.42 no.2
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• pp.139-143
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• 2009

We examined the vertical distribution of specific microbial groups and the patterns of microbial community structure within the soil profile using phospholipid fatty acid (PLFA). Samples were collected from the soil surface down to 15 cm in depth from paddy and upland fields located in Daegu, Korea. The two fields have been fertilized with only chemical fertilizers N, P, K for 33 years. Principal component analysis of the PLFA signatures indicated that the composition of the soil microbial communities changed significantly with the cultivation practices and soil depth, suggesting that cultivation practices of paddy and upland fields had more significant influence on soil microbial community than the soil depth did. The soil microbial communities changed more drastically with soil depth in upland field than in paddy field, with making thicker soil surface in paddy field in terms of soil microbial community. The ratios of cyclopropyl/monoenoic precursors and total saturated/total monounsaturated fatty acids increased with soil depth, suggesting that the deeper soil horizons are more carbon-limited and anaerobic than surface soil. The community analysis using PLFAs as biomarkers revealed that Gram-positive bacteria and actinomycetes tended to increase in proportional abundance with increasing soil depth, while the abundance of Gram-negative bacteria and fungi were highest at the soil surface and substantially lower in the subsurface.

• BMB Reports
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• v.38 no.3
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• pp.307-313
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• 2005

Thiobacillus ferrooxidans is one of the most important bacterium used in bioleaching, and can utilize $Fe^{2+}$ or sulphide as energy source. Growth curves for Thiobacillus ferrooxidans have been tested, which show lag, logarithmic, stationary and aging phases as seen in other bacteria. The logarithmic phases were from 10 to 32 hours for Thiobacillus ferrooxidans cultivated with $Fe^{2+}$ and from 4 to 12 days for Thiobacillus ferrooxidans cultivated with elemental sulphur. Differences of protein patterns of Thiobacillus ferrooxidans growing on elemental sulphur and $Fe^{2+}$ separately were investigated after cultivation at $30^{\circ}C$ by the analysis of two-dimensional gel electrophoresis (2-DE), matrix-assisted laser desorption/ ionization (MALDI)-Mass spectrometry and ESI-MS/MS. From the 7 identified protein spots, 11 spots were found more abundant when growing on elemental sulphur. By contrast 6 protein spots were found decreased at elemental cultivation condition. Among the proteins identified, cytochrome C have been previously identified as necessary elements of electron-transfering pathway for Thiobacillus ferrooxidans to oxidize $Fe^{2+}$; ATP synthase alpha chain and beta are expressed increased when Thiobacillus ferrooxidans cultivated with $Fe^{2+}$ as energy source. ATP synthase Beta chain is the catalytic subunit, and ATP synthase alpha chain is a regulatory subunit. The function of ATPase produces ATP from ADP in the presence of a proton gradient across the membrane.

• Proceedings of the KSRS Conference
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• 2003.11a
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• pp.426-428
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• 2003

Optical and microwave remote sensing data have been widely used in land cover and land use classification. Thanks to the spectral absorption characteristics of ground object in visible and near infrared region, optical data enables to extract different land cover types according to their material composition like water body, vegetation cover or bare land. On the other hand, microwave sensor receives backscatter radiance which contains information on surface roughness, object density and their 3-D structure that are very important complementary information to interpret land use and land cover. Separate use of these data have brought many successful results in practice. However, the accuracy of the land use / land cover established by this methodology still has some problems. One of the way to improve accuracy of the land use / land cover classification is just combination of both optical and microwave data in analysis. In this paper for the research, the author used LANDSAT TM scene 127/45 acquired on October 21, 1992, JERS-1 SAR scene 119/265 acquired on October 27, 1992 and aerial photographs taken on October 21, 1992. The study area has been selected in Hanoi City and surrounding area, Vietnam. This is a flat agricultural area with various land use types as water rice, secondary crops like maize, cassava, vegetables cultivation as cucumber, tomato etc. mixed with human settlement and some manufacture facilities as brick and ceramic factories. The use of only optical or microwave data could result in misclassification among some land use features as settlement and vegetables cultivation using frame stages. By combination of multitemporal JERS-1 SAR and TM data these errors have been eliminated so that accuracy of the final land use / land cover map has been improved. The paper describes a methodology for data combination and presents results achieved by the proposed approach.

• Korean Journal of Environmental Biology
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• v.38 no.4
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• pp.594-602
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• 2020

This study investigated the effect of geography and altitude on epigeic spider communities in rice field levees in Jeollabuk-do. Spider communities in the mountainous and plain areas were compared to determine the effect of geography on the cultivation periods. The effect of altitude was compared between the Jeongeup and Jangsu areas during non-cultivation periods. Analysis using nMDS (non-metric multidimensional scaling), MRPP (multiple response permutation procedure), and ANOSIM (analysis of similarity) revealed differences in spider community structures between the two types of study areas. Lycosidae predominated at the family level, and its abundance was greater in the mountainous area than in the plains area. The total abundance did not differ between the two areas with different altitudes, but the abundance of three Pardosa species was greater at lower altitudes than at higher altitudes. Geography and altitude had a minimal effect on species richness and diversity indices at the community level. However, several Lycosidae species showed species-specific responses to both geography and altitude in the rice fields.

• Smart Media Journal
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• v.11 no.5
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• pp.26-37
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• 2022

In this paper, images are automatically acquired to control the initial disease of strawberries among facility cultivation crops, and disease analysis is performed using the EfficientNet model to inform farmers of disease status, and disease diagnosis service is proposed by experts. It is possible to obtain an image of the strawberry growth stage and quickly receive expert feedback after transmitting the disease diagnosis analysis results to farmers applications using the learned EfficientNet model. As a data set, farmers who are actually operating facility cultivation were recruited and images were acquired using the system, and the problem of lack of data was solved by using the draft image taken with a cell phone. Experimental results show that the accuracy of EfficientNet B0 to B7 is similar, so we adopt B0 with the fastest inference speed. For performance improvement, Fine-tuning was performed using a pre-trained model with ImageNet, and rapid performance improvement was confirmed from 100 Epoch. The proposed service is expected to increase production by quickly detecting initial diseases.

• Mycobiology
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• v.51 no.1
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• pp.49-59
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• 2023

Antrodia cinnamomea, an edible and medicinal fungus with significant economic value and application prospects, is rich in terpenoids, benzenoids, lignans, polysaccharides, and benzoquinone, succinic and maleic derivatives. In this study, the transcriptome of A. cinnamomea cultured on the wood substrates of Cinnamomum glanduliferum (YZM), C. camphora (XZM), and C. kanehirae (NZM) was sequenced using the high-throughput sequencing technology Illumina HiSeq 2000, and the data were assembled by de novo strategy to obtain 78,729 Unigenes with an N50 of 4,463 bp. Compared with public databases, about 11,435, 6,947, and 5,994 Unigenes were annotated to the Non-Redundant (NR), Gene Ontology (GO), and Kyoto Encyclopedia of Genes and Genome (KEGG), respectively. The comprehensive analysis of the mycelium terpene biosynthesis-related genes in A. cinnamomea revealed that the expression of acetyl-CoA acetyltransferase (AACT), acyl-CoA dehydrogenase (MCAD), 3-hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA), mevalonate pyrophosphate decarboxylase (MVD), and isopentenyl diphosphate isomerase (IDI) was significantly higher on NZM compared to the other two wood substrates. Similarly, the expression of geranylgeranyltransferase (GGT) was significantly higher on YZM compared to NZM and XZM, and the expression of farnesyl transferase (FTase) was significantly higher on XZM. Furthermore, the expressions of 2,3-oxidized squalene cyclase (OCS), squalene synthase (SQS), and squalene epoxidase (SE) were significantly higher on NZM. Overall, this study provides a potential approach to explore the molecular regulation mechanism of terpenoid biosynthesis in A. cinnamomea.