• Title/Summary/Keyword: Crude carbohydrate

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A carbohydrate antigen of Clonorchis sinensis recognized by a species-specific monolonal antibody (종특이 단세포군항체에 반응하는 간흡충의 당질항원 한 가지)

  • 용태순;이종석
    • Parasites, Hosts and Diseases
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    • v.34 no.4
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    • pp.279-282
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    • 1996
  • The enzyme-linked immunosorbent assay (ELISA)-inhibition test using a Clonorchis sinensis species-specific mouse monoclonal antibody (MAb) , CsHyb 0605-23, showed increased specificity over the conventional ELISA used for serodiagnosis of clonorchiasis. To characterize the corresponding antigen further, the MAb was tested against polysaccharide, protein and glycolipid fractions obtained from a crude extract of C. slnensis adult worms, using chloroform, methanol and phenol extractions. Only the polysaccharide fraction was recognized by the mb among those fractions. Mild oxidation of the antigen with sodium periodate showed decreased reactivity against the MAb. We concluded that the antigen and antigenic determinants recognized by the MAb are carbohydrates.

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Dynamic changes and characterization of the protein and carbohydrate fractions of native grass grown in Inner Mongolia during ensiling and the aerobic stage

  • Du, Zhumei;Risu, Na;Gentu, Ge;Jia, Yushan;Cai, Yimin
    • Asian-Australasian Journal of Animal Sciences
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    • v.33 no.4
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    • pp.556-567
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    • 2020
  • Objective: To improve the utility of native grass resources as feed in China, we investigated the dynamics of protein and carbohydrate fractions among Inner Mongolian native grasses, during ensiling and the aerobic stage, using the Cornell Net Carbohydrate and Protein System. Methods: Silages were prepared without or with lactic acid bacteria (LAB) inoculant. We analyzed the protein and carbohydrate fractions and fermentation quality of silages at 0, 5, 15, 20, 30, and 60 d of ensiling, and the stability at 0.5, 2, 5, and 10 d during the aerobic stage. Results: Inner Mongolian native grass contained 10.8% crude protein (CP) and 3.6% water-soluble carbohydrates (WSC) on a dry matter basis. During ensiling, pH and CP and WSC content decreased (p<0.05), whereas lactic acid and ammonia nitrogen (N) content increased (p<0.05). Non-protein N (PA) content increased significantly, whereas rapidly degraded true protein (PB1), intermediately degraded true protein (PB2), total carbohydrate (CHO), sugars (CA), starch (CB1), and degradable cell wall carbohydrate (CB2) content decreased during ensiling (p<0.05). At 30 d of ensiling, control and LAB-treated silages were well preserved and had lower pH (<4.2) and ammonia-N content (<0.4 g/kg of fresh matter [FM]) and higher lactic acid content (>1.0% of FM). During the aerobic stage, CP, extract ether, WSC, lactic acid, acetic acid, PB1, PB2, true protein degraded slowly (PB3), CHO, CA, CB1, and CB2 content decreased significantly in all silages, whereas pH, ammonia-N, PA, and bound true protein (PC) content increased significantly. Conclusion: Control and LAB-treated silages produced similar results in terms of fermentation quality, aerobic stability, and protein and carbohydrate fractions. Inner Mongolian native grass produced good silage, nutrients were preserved during ensiling and protein and carbohydrate losses largely occurred during the aerobic stage.

Effects of Bacterial Inoculants and Organic Acids on Silage Quality : Meta-analysis (미생물제제 및 유기산제제의 처리가 사일리지 품질에 미치는 영향 : 메타분석)

  • Cho, Sangbuem;Kwon, Chan Ho;Kim, Eun Joong
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.34 no.2
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    • pp.94-102
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    • 2014
  • This study was conducted to estimate and compare the effects of bacterial inoculants and organic acids on silage quality. Silage pH, lactate, acetate, lactate:acetate ratio, propionate, butyrate, water-soluble carbohydrate, crude protein, ammonia-N, neutral detergent fiber and acid detergent fiber (ADF) were used as parameters for quality analysis and a meta-analysis technique was employed to determine the effect size. As a data pool for analysis, we examined 14 research papers. Bacterial inoculants were found to elevate pH, lactate, acetate, lactate:acetate ratio, propionate and ADF contents compared to the controls (p<0.01). In contrast bacterial inoclulants decreased butyrate, water-soluble carbohydrate, crude protein and ammonia-N contents (p<0.01). In the organic acid treatments, all parameters except ADF showed higher contents than the control (p<0.01). In the comparison of effect sizes between the two treatments, significant differences were detected in butyrate, water-soluble carbohydrate, crude protein and ammonia-N (p<0.05). It may be concluded that bacterial inoculants could improve silage quality in terms of the aforementioned four parameters compared with organic acid treatments.

Components Analysis of Korean Pollens and Pollen Extracts (국내산 화분 및 화분 추출물의 성분 분석)

  • Lee, Boo-Yong;Choi, Hee-Don;Hwang, Jin-Bong
    • Korean Journal of Food Science and Technology
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    • v.29 no.5
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    • pp.869-875
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    • 1997
  • The chemical components of Korean pollens and pollen extracts were analysed to provide fundamental data for pollen processing and products development. The contents of crude protein, crude lipid, crude ash, crude fiber and carbohydrate of pollens were $14.0{\sim}22.3%,\;3.0{\sim}8.8%,\;2.2{\sim}3.3%,\;0{\sim}37.5%,\;and\;31.9{\sim}64.4%$ respectively, showing the specially high crude fiber content (37.5%) in Pine pollen. Almost all the carbohydrate, lipid, protein and ash components except fiber were extracted by water and n-hexane. Free sugars identified in water extracts $(10\;^{\circ}Bx)$ of pollens were fructose and glucose as major component and maltose as minor component. The proportions of three free sugars to total soluble solid content of Mixed, Acorn, and Pine water extracts were 73.6%, 85.4% and 47.7% respectively. Potassium and phosphorus content in pollens and pollen water extracts were high, but Ca, Na, Mg, Fe and Zn were not major mineral components. The essential amino acids such as leucine, phenylalanine, methionine, lysine, valine, isoleucine and threonine were contained richly in pollens and those were almost completely extracted by water. Vitamin $B_1$ and C were not detected, but only small amount of vitamin $B_2$ was detected in the pollens and pollen extracts tested. The essential fatty acids such as linoleic acid and linolenic acid were contained richly in pollens. The proportion of unsaturated fatty acids to total fatty acid content in Mixed, Acorn and Darae pollen except Pine was higher than 50%.

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Quality Characteristics of Jelly Added with Pressed Kiwi(Actinidia chinensis var. 'Halla Gold') Juice (참다래 과즙을 첨가한 젤리의 품질 특성)

  • Oh, Hyun-Jeong;Back, Jin-Woo;Lee, Ju-Yeon;Oh, Young-Ju;Lim, Sang-Bin
    • Culinary science and hospitality research
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    • v.19 no.5
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    • pp.110-120
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    • 2013
  • Five types of kiwi jelly were prepared with different rates of pressed kiwi juice and carrageenan, and their quality characteristics such as chemical composition, saccharinity, color, texture, and sensory attributes were measured. There were no significant changes in moisture, crude lipid, carbohydrate, but significant differences were shown in crude ash, crude protein, pH, and acidity. The pH decreased and acidity increased as the amount of added kiwi juice increased. The saccharinity of kiwi jelly increased with increased amount of added kiwi juice. Additionally, the total phenolic content, DPPH radical scavenging activity and content increased with increased amount of added kiwi juice. The hardness, gumminess, and chewiness increased with increased levels of carrageenan. As the amount of added carrageenan increased, the L values of kiwi jelly decreased. Based on the sensory evaluation test, kiwi jelly, JKJ(Jeju Kiwi Jelly)-3 was the best in the flavor, sweetness, texture, and overall acceptability. The chemical composition of JKJ-3 was as follows : moisture $75.1{\pm}0.5%$, carbohydrate $24.2{\pm}0.5%$, crude protein $0.29{\pm}0.05%$, and crude ash $0.44{\pm}0.02%$. The kiwi jelly, JKJ-3 of overall acceptability values in the sensory test for flavor, sweetness, hardness, texture and overall favorite were 3.65, 3.35, 3.35, 3.50 and 3.60, respectively, with the addition of 20% pressed kiwi juice and 2.3% carrageenan.

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Effect of Nutritional Difference between Soy Milk and Mung Milk on Fermentation

  • Gyeongseon An;Yeonghun Cho;Jungmin Ha
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2022.10a
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    • pp.301-301
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    • 2022
  • Dairy products are important diet source for human because of their balanced essential nutrients along with various vitamins and minerals. However, lactose in milk can result in diarrhea to some consumers with lactose intolerance. Soy milk has no lactose and is suitable as a substitute for diary milk in accordance with recent trend of replacing animal food with vegetable food. However, polysaccharides in soy milks are difficult for humans to digest, leading to flatulence. These polysaccharides can be decomposed into monosaccharides by lactic acid bacteria, and fermentation can improve food quality. Because mungbean has higher carbohydrate content than soybean, mung milk can be easily fermented than soy milk, resulting in vege yogurt with higher contents of lactic acid. In this study, fermentation characteristics of vege yogurt were analyzed with different ratio of soy milk and mung milk (0%, 25%, 50%, 75%, 100% and 0%+sucrose) and different fermentation time (0, 8, and 16 hours). In general, pH decreased as fermentation time increased. Overall, pH significantly decreased when the mung milk content in yogurt increased. All samples showed higher titratable acidity after fermentation and soy yogurt (mungbean 0%, 16 hours) with sucrose showed the highest value (6.825%). As fermentation time increase, viscosity increased. In 8 and 16 hours, mung milk yogurt (mungbean 100%) showed the lowest viscosity while soy milk yogurt (soybean 100%) with no sucrose showed the highest viscosity after 16 hours of fermentation. The contents of crude protein, crude fat and ash were measured for nutritional analysis. Soy milk (mungbean 0%, 0 hours) had the values of crude protein 2.9g, crude fat 1.8g, and ash 0.3g, and mung milk (mungbean 100%, 0 hours), showed the values of crude protein 1.7g, crude fat 0g, and ash 0.3g. To analyze the effect of the differences in the contents of nutrition between soy milk and mung milk on fermentation, the changes in sugar contents, and antioxidant capacity will be conducted depending on fermentation time. Our results will provide the information in researching plant beverages.

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Effect of Secondary Nutrients on the Leaf Yield and Biochemical Constituents of Terminalia tomentosa

  • Sinha, U.S.P.;Prasad, J.;Das, S.;Ojha, N.G.;Vijay Prakash, N.B.
    • International Journal of Industrial Entomology and Biomaterials
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    • v.18 no.2
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    • pp.135-138
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    • 2009
  • Studies on the effect of different secondary nutrients in different doses on the leaf yield and biochemical constituents of Terminalia tomentosa revealed that secondary nutrients have promotary effect in increasing the leaf yield and foliar constituents of Terminalia tomentosa. Among all the treatments under study, foliar application of magnesium sulphate (2g/plant, w/v) is the best in respect of leaf yield and biochemical constituents of T. tomentosa. This treatment gave 29.15% increase in leaf yield over control. Chemoassay results further confirmed significant improvement in biochemical constituents. Except crude fibre, moisture, minerals, crude protein and total carbohydrate increased significantly over control.

A Study on the General Components and Minerals in Parts of Omija (Schizandra Chinensis Baillon) (오미자의 부위별 일반성분과 무기질함량에 관한 연구)

  • Lee, Joung-Sook;Lee, Mi-Gyeung;Lee, Sung-Woo
    • Journal of the Korean Society of Food Culture
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    • v.4 no.2
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    • pp.173-176
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    • 1989
  • This study was carried out to comparison of the contents of general and mineral components between the original sample and water extract in each part of omija. The content of Carbohydrate was highest in endocarps, and that the crude protein and crude lipid in seeds. The contents of K and Mn in the fruits, endocarps, and seeds were all higher than those of the other cations. The content of K and Zn in endocarps were three to four times as much as those of seeds. and the content of Na, Ca, and Cu in endocarps were 1.5 to 1.75 times of seeds. Mineral contents by water extract in each part were ordered as K, Mg, Ca, and Fe. As the percentage of each ion in water extract on the basis of original sample, Fe was the highest ratio of behavior, and Mn was lowest.

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Mineral Contents and Fatty Acid Composition of Jemsosojoo (흑염소 소주의 무기질 함량과 지방산 조성)

  • 김종수;김관필;이만종
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.27 no.2
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    • pp.220-226
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    • 1998
  • This study was undertaken to analyze proximate comosition, mineral contents and fatty acid composition of pure jemsosojoo(PJ) and jemsosojoo added medicinal herb(JMH). Mositure, ash, crude protein and crude fat of pure jemsosojoo were higher than those of the JMH, but carbohydrate content was higher in the JMH. Among minerals, Ca, Mg, Fe, Zn content of the JMH were higher than those of the PJ, while P and Na were higher in the PJ and those difference were significant between the PJ and the JMH(p<0.05). U/S (unsaturaed fatty acid/saturated fatty acid) ratio was higher in the JMH than those of the PJ, the ratio o fglycolipid was 1.75 in the PJ and that of neutral lipid was 3.07 in the JMH. Octadecenoic acid, hexadecanoic acid, octadecenoic acid and hexadecenoic acid were major fatty acids in the total lipids, neutral lipids glycolipids and phospolipids of the PJ and the JMH. Especially, octadecadienoic acid and octadecatrienoic acid were higher in the JMH than those of the PJ.

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Changes in Physico-chemical Properties and Mineral Contents during Buckwheat Germination (메밀 발아 중 물리화학적 특성과 무기질 함량의 변화)

  • 이명헌;손흥수
    • The Korean Journal of Food And Nutrition
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    • v.7 no.4
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    • pp.267-273
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    • 1994
  • To provide the effective application scheme and basic information of buckwheat (Fagopyrum esculentum Moench), buckwheat was germinated at 10$^{\circ}C$ for 7 days and 100 kernels weight, germination rate, root length, chemical composition and mineral contents were examined at 24 hour Intervals. During the germination period, the 100 kernels weight increased approximately 0.3g per day. The germination rate increased sharply after 2 days and the root length increased greatly after 4 days. The crude protein contents increased with germination time, whereas the carbohydrate contents decreased. The crude ash and fat contents did not differ significantly during the germination period. The Ca contents Increased for the 4th day of gemination, but gradually decreased afterwords. The Na contents increased in the initial stage of germination, but then gradually decreased. However, there were no significant change In the Mg, K. Fe. Mn and Zn contents.

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