• Title/Summary/Keyword: Crossing Culture

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Selection of Superior Strains of Cordyceps militaris with Enhanced Fruiting Body Productivity

  • Sung, Jae-Mo;Park, Young-Jin;Lee, Je-O;Han, Sang-Kuk;Lee, Won-Ho;Choi, Sung-Keun;Shrestha, Bhushan
    • Mycobiology
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    • v.34 no.3
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    • pp.131-137
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    • 2006
  • In vitro fruiting bodies were produced from ten different isolates of Condyceps militaris EFCC C-5736, EFCC C-5941, EFCC C-5976, EFCC C-6040, EFCC C-6849, EFCC C-7268, EFCC C-7342, EFCC C-7992, EFCC C-8027 and EFCC C-8549. Single ascospores were isolated from in vitro grown fruiting bodies and used for fruiting body production in brown rice medium by both intra-strain crossing and out-crossing. Length and dry wt. of stromata grown in vitro were measured. Strains producing highest dry wt. of stromata were selected. Both intra-strain crossings and inter-strain crossings of single ascospore strains were found to produce profuse fruiting bodies of C. militaris.

Culture study on the hybrid by interspecific crossing between Porphyra pseudolinearis and P. dentata (Bangiales, Rhodophyta), two dioecious species in culture

  • Kim, Nam-Gil
    • ALGAE
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    • v.26 no.1
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    • pp.79-86
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    • 2011
  • Physiological studies on the hybrid by crossing between two dioecious species, Porphyra pseudolinearis and P. dentata from Korea were conducted at constant temperatures (5, 10, 15, 20, and $25^{\circ}C$), at photon flux densities (10, 20, 40, and $80\;{\mu}mol\;m^{-2}s^{-1}$) under photoperiods (14 L : 10 D and 10 L : 14 D). In the hybrid, higher growth of conchocelis was observed at 20 and $40\;{\mu}mol\;m^{-2}s^{-1}$ under 14 L : 10 D. Conchosporangial branches were produced under $10-80\;{\mu}mol\;m^{-2}s^{-1}$ at only $25^{\circ}C$, and were abundant when the conchocelis was cultured under 10 L : 14 D. Foliose thalli of the hybrid grew well at the conditions of $10-20^{\circ}C$, 10 L : 14 D and $15-20^{\circ}C$, 14 L : 10 D. The foliose thalli grew very slowly at $5^{\circ}C$ and $30^{\circ}C$, respectively. No archeospores were observed at any culture conditions. Spermatangial and zygotosporangial sori were formed at the marginal portion of mature thallus. Zygotospores from the hybrid were released at $10-2^{\circ}C$ under both photoperiods, and gave rise to form conchocelis filament. Monoecious thalli were observed at $10^{\circ}C$ under 14 L : 10 D. Neither monospores nor protothalli were produced from the conchocelis in culture.

Evaluation on Structural Stability of Railway Level Crossing System using Rubber Panel by High Speed Train Gust (고무보판 패널 철도건널목 시스템의 고속열차 풍하중에 대한 구조 안정성 분석)

  • Choi, Jung-Youl;Kim, Sang-Jin;Shin, Tae-Hyoung;Chung, Jee-Seung
    • The Journal of the Convergence on Culture Technology
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    • v.5 no.2
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    • pp.355-360
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    • 2019
  • In this study, the structural stability of the railway level crossing system using rubber panel for high speed lines was investigated by applying the specification for wind load conditions (Train gust) of high speed train (300km/h and 360km/h). A finite element analysis using three-dimensional modeling was carried out by applying the field conditions that was installed with the complicated configuration of the rubber plate panel system. As a result of this study, the structural stability of the rubber plate panel system for high speed train gust was analytically verified.

The New Paradigm of Circular Economy and Crossing-culture In the Network-manufacture System

  • Yang, Xiao-Yuan;Huang, Chuan-Kun
    • Proceedings of the Korea Society for Industrial Systems Conference
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    • 2007.02a
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    • pp.181-188
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    • 2007
  • In this paper, the management thoughts of The network-manufacture based on virtual-agile organization is Brief summarized, then an explanation had been made about the virtual circulation economy patterns paradigm, which is integrated the virtual-agile manufacture and circulation economy theory, the critical content of this paradigm, at last we recognized the virtual-agile organization pattern circulation economy is one important trend of the network-manufacture development in the future.

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Effect of Korea's Entertainment Culture on the Fashion of the College Students (우리나라 놀이 문화가 대학생 패션에 미친 영향)

  • Yang, A-Rang;Lee, Hyo-Jin
    • The Research Journal of the Costume Culture
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    • v.16 no.1
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    • pp.70-87
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    • 2008
  • The purpose of this study is to examine how the entertainment culture has influenced the fashion of the college students since the year 2000, and to analyze the fashion trends of those who enjoy their entertainment culture. The entertainment culture is, therefore, perceived in the most sensitive way by the college students, who belong to the latter period of their adolescence, while influencing them. Especially recently, the entertainment culture of the college students has emerged as a new symbol of the overall fashion. And its effect on the fashion has become conspicuous. The 21st entertainment culture is comprised of "club culture", which is an off-line fad, and "on-line game culture". The result of this study is as follows: Firstly, the formative way that the so-called "club culture" influenced the fashion of the college students shows that their overall fashion trends are characterized by a 'caports image' and a 'childlike-amusing image'. Secondly, the formative fashion style, influenced by the so-called "on-line game culture", presents a 'post- structural image' by using heterogeneous material in order to introduce a new point of view, and by transforming the creation style and design. And it also presents a 'homosexual image' by crossing the attractiveness of both genders. This study is baseds on the documents study.

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Damage Analysis of Manganese Crossings for Turnout System of Sleeper Floating Tracks on Urban Transit (도시철도 침목플로팅궤도 분기기 망간크로싱의 손상해석)

  • Choi, Jung-Youl;Yoon, Young-Sun;Ahn, Dae-Hee;Han, Jae-Min;Chung, Jee-Seung
    • The Journal of the Convergence on Culture Technology
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    • v.8 no.5
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    • pp.515-524
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    • 2022
  • The turnout system of the sleeper floating tracks (STEDEF) on urban transit is a Anti-vibration track composed of a wooden sleeper embedded in a concrete bed and a sleeper resilience pad under the sleeper. Therefore, deterioration and changes in spring stiffness of the sleeper resilience pad could be cause changes in sleeper support conditions. The damage amount of manganese crossings that occurred during the current service period of about 21 years was investigated to be about 17% of the total amount of crossings, and it was analyzed that the damage amount increased after 15 years of use (accumulated passing tonnage of about 550 million tons). In this study, parameter analysis (wheel position, sleeper support condition, and dynamic wheel load) was performed using a three-dimensional numerical model that simulated real manganese crossing and wheel profile, to analyze the damage type and cause of manganese crossing that occurred in the actual field. As a result of this study, when the voided sleeper occurred in the sleeper around the nose, the stress generated in the crossing nose exceeded the yield strength according to the dynamic wheel load considering the design track impact factor. In addition, the analysis results were evaluated to be in good agreement with the location of damage that occurred in the actual field. Therefore, in order to minimize the damage of the manganese crossing, it is necessary to keep the sleeper support condition around the nose part constant. In addition, by considering the uniformity of the boundary conditions under the sleepers, it was analyzed that it would be advantageous to to replace the sleeper resilience pad together when replacing the damaged manganese crossing.

Studies on the Interspecific and Intergeneric Hybridization in Herbage Grasses I. Effects of hybrid embryo age on callus formation and plant regeneration (화본과목초의 종.속간 잡종에 관한 연구 I. 교잡* 일령에 따른 Callus형성과 식물체재분화)

  • 박병훈;김명환
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.9 no.2
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    • pp.62-67
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    • 1989
  • A breeding program in progress at Suweon Livestock Experiment Station, RDA, involves the hybridization of italian ryegrass (Lm) and several leading forage grasses in an effort to combine the nutritive, productivity and palatability qualities of Lm with the adaptive and cold resistance qual; .les of several leading grasses. In order to study the fate of the hybridization between remotely related species, immatured hybrid embryos were cultured on media. The emasculated plants of Lm were Sikem and Tetrone. Reweille, 2n=14, of perennial ryegrass (Lp.), Forager, 2n=42, of tall fescue(Fa), First, 2n=14,of meadow fescue(Fp), Potomac, 2n=28, of orchardgrass(Dg), and Richmond, 2n=42, of timothy(Ph.p) were used as pollinators. Embryos were isolated on 4, 8, 12, 16, and 20 days after pollination and cultured them on modified Murashige and Skoog media. Calluses and plantlets have been obtained after 8 days old embryos crossing between $Lm{\times}Fa$, after 12 days embryos crossing between $Lm{\times}Lp$, Dg, and Ph.p, and after 16 days embryos crossing between $Lm{\times}Fp$. Both callus and shoot formation occurred on 6 % or less of the plated embryos of $Lm{\times}Fp$, Dg, and Ph.p. Embryoderived callus forming shoots have been obtained from 4 days old embryo crossing between $Lm{\times}Dg$ which has not successfully been done anywhere as far as we know. It means that hybrid plants of species without crossability can be obtained through the use of immatured embryo culture. Some of plated embryos developed directly shoots from embryos and the others shoot-forming callus. Cross between related species showed a high frequency of directly shoot formation from plated embryos and cross between remotely related species a high frequency of callus formation.

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Breeding of a New Light Pink Spray-mum 'Noble ND' as Cut Flower (연분홍색의 절화용 스프레이국화 'Noble ND' 육성)

  • Kim, Hyun-Seok;Seong, Jeon-Joong;Han, Youn-Yul;Choi, Dong-Jin;Woo, Jin-Ha
    • Horticultural Science & Technology
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    • v.28 no.5
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    • pp.895-898
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    • 2010
  • A new light pink spray-mum, 'Noble ND' was bred by crossing 'Mona Lisa', a white anemone type flower, and 'Dark Weldon', a pink anemone type flower in October, 2004. The growth and flowering characteristics were investigated in forcing, natural, and retarding cultures from 2006 to 2007. The 'Noble ND' was a medium-flowering fall-mum with light pink anemone type flower and had 9.2 flowers per stem, with 4.8 cm flower diameter in natural culture. The number of days to flowering and flowers per plant was 57 and 7.4, respectively, in forcing culture of 'Noble ND', whereas the number of days to flowering was 48 in retarding culture.

Breeding of a New Light Pink Spray-mum 'Brown ND' for Cut Flower (연분홍색의 절화용 스프레이국화 '브라운엔디' 육성)

  • Kim, Hyun Seok;Han, Youn Yul;Seong, Jeon Joong;Choi, Dong Jin
    • FLOWER RESEARCH JOURNAL
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    • v.18 no.1
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    • pp.73-76
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    • 2010
  • A new light pink spray-mum, 'Brown ND' was bred by crossing between 'Noeul', a yellow anemone type flower, and 'Dalma', a pink anemone type flower in October 2003. The growth and flowering characteristics were investigated in forcing, natural, and retarding cultures from 2005 to 2007. The 'Brown ND' was a medium-flowering fall-mum with light pink anemone type flower and had 10.6 flowers per stem, 4.1 cm diameter of flower in natural culture. The number of days to flowering and flowers per plant was 59 and 9.1, respectively, in forcing culture of 'Brown ND' whereas the number of days to flowering was 49 in retarding culture.

Breeding of a New White Spray-mum 'White Ball' for Cut Flower (백색의 절화용 스프레이국화 '화이트볼' 육성)

  • Kim, Hyun Seok;Han, Youn Yul;Seong, Jeon Joong;Choi, Dong Jin
    • FLOWER RESEARCH JOURNAL
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    • v.18 no.1
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    • pp.77-81
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    • 2010
  • A new white spray-mum, 'White Ball' was bred by crossing between 'Stallion', a white anemone type flower, and 'Hyangno', an anemone type with pink petals in October 2003. The growth and flowering characteristics were investigated in forcing, natural, and retarding cultures from 2005 to 2007. The 'White Ball' was a medium-flowering fall-mum with white anemone type flower and had 16.2 flowers per stem, 3.6 cm diameter of flower in natural culture. The number of days to flowering and flowers per plant was 58.5 and 11.9, respectively, in forcing culture of 'White Ball' whereas the number of days to flowering was 54 in retarding culture.