• 한국환경생물학회:학술대회논문집
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• 한국환경생물학회 2002년도 학술대회
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• pp.127-131
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• 2002

The OJIP transients were investigated from leaves of Crinum asiaticum var. japonicum, Osmanthus insularis, Chloranthus glaber and Asplenium antiquum, in order to screen the indicators for quantification of environmental stresses and to apply them to the plant vitality test. The obvious diurnal changes or OJIP transients were found in Crinum asiaticum var. japonicum, Osmanthus insularis growing in sunny habitats, showing lower Fp and higher Fo in day time. The values of SF $I_{no}$ and P $I_{NO}$ increased dramatically in day time in leaves of Crinum asiaticum var. japonicum, Osmanthus insularis, and Asplenium antiquum. Although the value of RC/CS decreased slightly in day time in leaves of 4 tropica $l_tropical plants, the obvious diurnal changes or OJIP transients were not seen in winter, suggesting that tropica $l_tropical paints used in this research seemed to be irreversibly damaged in winter. However, the values of ETo/RC and Ψo/(1-Ψo) increased significantly in leaves of Crinum asiaticum var. japonicum, indicating that ETO/RC and Ψo/(1-Ψo) were useful as indicator parameters for scensing of low temperature.ature.

• 생약학회지
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• 제48권2호
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• pp.148-154
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• 2017

Crinum asiaticum var. japonicum and its active component, norgalanthamine have been reported to have hair growth-promoting effect via the proliferation of dermal papilla cells. In this study, we investigated the other mechanisms of C. asiaticum extract var. japonicum and norgalanthamine on the hair growth. The C. asiaticum var. japonicum extract inhibited $5{\alpha}$-reductase activity by 16%, which converts testosterone to dihydrotestosterone (DHT), a main cause of androgenetic alopecia, whereas the C. asiaticum var. japonicum extract didn't function as an opener of the $K_{ATP}$ channel. On the other hand, we examined whether norgalanthamine can inhibit transforming growth factor-${\beta}$ (TGF-${\beta}$) signal pathway, which is essential in the regression induction of hair growth. Norgalanthamine inhibited the phosphorylation of Smad2/3 on TGF-${\beta}1$-induced canonical pathway in human keratinocyte HaCaT cells. These results suggested that the C. asiaticum var. japonicum extract and norgalanthamine had the potential to influence hair growth through the inhibition of $5{\alpha}$-reductase activity and TGF-${\beta}1$-induced canonical pathway.

• 생약학회지
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• 제26권2호
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• pp.139-147
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• 1995

Crinum asiaticum var. japonicum is a wild plant growing only in Jeju-island, Korea, and in Japan. The whole part of this plant has been known to have the pharmacological actions such as analgesic, anti-inflammatory, platelet-aggregation inhibitory, antitussive, and expectorant. With these assumed actions, the leaves (Crinum folium) of this plant has been used in the folk remedies for arthritis and arthralgia. There is, however, no scientific evidences for the pharmacological actions of Crinum asiaticum var. japonicum. In the present study, the analgesic, anti-inflammatory, and platelet-aggregation inhibitory actions of Crinium folium were evaluated using writhing test, tail-flick test, carrageenin antiedema test, in vitro thromboxane $B_2$ quantitation assay and in vitro platelet aggregation test. In order to obtain the partially purified fraction whose pharmacological action is excellent, the methanol extract of Crinium folium was fractionated consecutively into four biological fractions such as ether, ethyl acetate, butanol, and water fractions and their pharmacological actions of the fractions were investigated. Putting our results together, Crinium folium, especially ethyl acetate fraction was proven to have significant analgesic, anti-inflammatory and platelet-aggregation inhibitory actions by inhibition of prostanoids biosynthesis as one of its mechanism of action.

• 대한화장품학회지
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• 제32권1호
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• pp.59-64
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• 2006

문주란(Crinum asiaticum Linne var. japonicum)은 한국, 말레이지아 등 동남아시아 지역에서 관절통, 해열, 궤양 치료, 국부의 소염 및 해열 등의 목적에 민간요법 등으로 오래 전부터 사용되어 오고 있다. 본 연구에서는 이러한 문주란의 화장료 조성물로서의 가능성을 확인하기 위하여 iNOS (inducible nitric oxide synthase) 억제 그리고 PGE2, IL-6, and IL-8 방출 억제에 의한 항염 효능을 측정하였다. pH를 3.5로 조절하고 95% ethanol을 사용하여 추출한 후, HPLC 실험으로 문주란의 주성분이 면역조절물질로 잘 알려진 lycorine이며 대략 1% 정도 함유한 것을 확인하였고 그 함량은 문주란의 추출 부위 및 추출방법에 따라 달랐다. 리포다당(lipopolysaccharide, LPS)에 의해 활성화된 생쥐 대식세포(RAW 264.7 cells)에서 생성되는 NO 형성의 억제능을 측정한 항염실험에서, 문주란 에탄을 추출물은 투여량에 비례하는 저해능을 가지고 있음을 확인할 수 있었다($IC_{50} = 83.5 {\mu}g/mL$). 또한 RT-PCR법을 이용하여 문주란 에탄을 추출물이 iNOS 유전자 발현도 억제함을 확인하였다. 또한, 문주란 추출물은 전혀 세포독성을 보이지 않았으며 오히려 LPS에 대하여 세포증식효과를 나타내었다(세포생존율 약 $10{\sim}60%$ 증가). 인간의 섬유아세포에서 과산화수소에 의해 활성화된 PGE2, IL-6 및 IL-8 방출 억제효능 측정 실험에서는 0.05%와 1% 농도 이상에서 (PGE2와 IL-6의 분비가) 거의 완전히 억제됨을 확인할 수 있었고, 나아가서 IL-8의 경우는 모든 실험농도 범위에서 완전히 억제되었다(>0.0025%). 이러한 결과로부터 문주란의 추출물이 충분한 항염 효과를 가지고 있음을 확인할 수 있었다.

• 한국환경과학회지
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• 제14권4호
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• pp.389-395
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• 2005

The changes of O-J-I-P transients were investigated using leaves of four subtropical plant species (Crinum asiaticum var. japonicum, Osmanthus insularis, Chloranthus glaber and Asplenium antiquum) under the natural conditions in winter, in order to select the stress indicators for diagnosing physiological states of plants under low temperature. In the O-J-I-P transients of these species, the fluorescence intensity was found to be higher in O-step and lower in P-step in winter than in summer. Particularly, the fluorescence intensity of the P-step in Crinum asiaticum var. japonicum was lower than those of other three plant species, indicating that Crinum asiaticum var. japonicum is the most sensitive to low temperature. Of the chlorophyll fluorescence parameters derived from O-J-I-P transients of four subtropical plants, Fm, Fv/Fo, ABS/CS, TRo/CS, $\phi_{po}$ and $\phi_{po}/(1-\phi_{po})$ decreased significantly with the increase of Fo, Sm, N, EToICS, ETo/RC and $\psi_o/(1-\psi_o)$ depending on temperature drop in winter. Therefore, these parameters could be used as indicators for estimating low temperature stress and diagnosing physiological states of plants under the natural conditions in winter.

• 환경생물
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• 제22권1호
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• pp.233-241
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• 2004

겨울철 저온에 의한 식물의 반응 및 생리적 상태를 확인하기 위해 문주란(Crinum asiaticum var. japonicum)을 대상으로 엽록소형광과 항산화효소 찬성의 변화를 조사하였다. O-J-I-P곡선을 분석한 결과 겨울철에 J, I, P-단계에서의 형광세기가 현저하게 간소하였다. 문주란 잎의 광계II의 광화학적 효율, 즉 Fv/Fm은 겨울철의 온도변화 추이와 유사하게 낮은 값을 나타내어 겨울철 저온이 스트레스 요인으로. 작용하는 것으로 보인다. 그리고, 겨울철에 superoxide dismutase, ascorbate peroxidase, peroxidase 활성 이 다소 증가하였으나 catalase 활성은 여름철에 비하여 크게 낮았다. 이러한 결과는 겨울철의 Fv/Fm의 감소와 함께 문주란이 겨울철 저온에 의해 산화적 스트레스에 처해 있게되며 세포사멸이 일어나는 것으로 해석된다.

• 환경생물
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• 제23권2호
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• pp.206-213
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• 2005

본 연구는 환경요인의 변화, 특히 겨울철 저온이 식물체에 미치는 영향을 정량적으로 밝히기 위해, 저온감수성 식물인 문주란을 대상으로 겨울철 일주기에 있어서 항산화효소 활성과 O-J-I-P곡선의 변화를 조사하였다. 겨울철의 superoxide dismutase와 peroxidase의 활성은 여름철에 비해 다소 증가하였다. 특히, peroxidase는 겨울철 새벽과 밤에 높은 활성을 보였으며 초겨울에만 특이적으로 검출되는 isoenzyme들로 관찰할 수 있었다. 또한 겨울철 문주란 잎의 O-J-I-P곡선에서 J, I, P-단계의 형광세기가 여름철에 비해 현저하게 감소하였다. 그리고, O-J-I-P곡선으로부터 산출된 주요 형광변수들 중에 Fm과 $\Phi_{po}$는 겨울철 저온에 노출되는 기간이 길어짐에 따라 각각 $30\%$와 $50\%$로 감소하였으며, ABS/RC는 초겨울보다는 늦은 겨울에 2배정도 증가하였다.

• 한국환경과학회지
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• 제13권4호
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• pp.359-365
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• 2004

The change of chlorophyll fluorescence parameters, O-J-I-P transients and psbA gene expression were investigated in the leaves of Crinum asiaticum var. japonicum on the natural condition in winter, in order to elucidate physiological responses of photosystem II (PS II) activity to winter stresses. The photochemical efficiencies of PS II, Fv/Fm, were significantly low in winter, contrary to its high value in summer. The values of I -qN and I-qP were lower in midday than at dawn or night both in summer and winter, although their decrease in midday was less in winter than in summer. In the O-J-I-P transients, the fluorescence intensity of J, I, P-step decreased remarkably depending on temperature drop in winter. And the D I reaction center protein of PS II decreased in late winter more than in early winter, concomitantly with relatively high content of description products of psbA gene in midday. These results indicate that low temperature in winter causes irreversible damage to PS II and subsequently leads to cell death.

• 한국환경과학회지
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• 제16권5호
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• pp.633-640
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• 2007

The diurnal changes of chlorophyll fluorescence and antioxidative enzyme activity were investigated in the leaves from four subtropical plant species (Crinum asiaticum var. japonicum Bak., Osmanthus insularis Koidz., Asplenium antiquum Makino and Chloranthus glaber Makino) under the natural habitats in summer and winter. The intensity of chlorophyll fluorescence was lower in O-, I-, J-, P-steps of O-J-I-P transient in winter than summer, and prominent diurnal change was not found in the fluorescence intensity of four subtropical plant species in winter. The activity and isoenzyme pattern of SOD and catalase did irregularly change seasonally and diurnally in four subtropical plant species. In contrast, the peroxidase activity and isoenzyme pattern was different depending on plant species and growth seasons; The activity increased slightly more in winter than in summer in four subtropical plant species, and several isoenzymes appeared in the leaves from C. asiaticum var japonicum, O. insularis and A. antiquum in winter.

• 약학회지
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• 제52권1호
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• pp.1-6
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• 2008

The present study investigated the antiproliferative effects of Crnum asiaticum var. japonicum against HL-60 human leukemia cells. The 80% MeOH extract or several solvent fractions from the C. asiaticum inhibited the growth of HL-60 cells, whereas the growth of HEL-299 cells, human embryonic lung fibroblast, was scarcely inhibited. When the HL-60 cells were treated with the $CHCl_3$ fraction, the BuOH fraction, the EtOAc fraction and the $H_2O$ fraction, DNA ladder, chromatin condensation and increase of sub-G1 hypodiploid cells were observed. Furthermore, the $CHCl_3$ fraction and the BuOH fraction reduced Bc1-2 mRNA level, whereas Bax mRNA level was increased. These results suggest that the inhibitory effect of C. asiaticum on the growth of the HL-60 cell might be mediated through the induction of apoptosis via the down-regulation of Bc1-2. Taken together, components of C. asiaticum might have a therapeutic potential for the treatment of human leukemia.