• 대한의생명과학회지
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• 제13권1호
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• pp.17-23
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• 2007

To consider potentially new sources which have hypoglycemic effect and accelerating alcohol detoxification, this study was designed to investigate the effect of Crataegus fructus and Morns alba L. in alcohol-treated rats. I compared the body weight, glucose, aspartate aminotransferase (AST), alanine aminotransferase (ALT), alcohol dehydrogenase (ADH), and aldehyde dehydrogenase (ALDH) of rats administered both alcohol and extract of experimental plants to rats treated with alcohol alone. Administration of extracts of C. fructus and M alba, respectively, resulted in a significant reduction in the blood glucose level and the activities of ADH of liver compared to the control rats, and administration of extract of M. alba showed significantly lower on bodyweight gain in the rats than in other treated rats. In contrast, the activities of ALDH of liver were increased. The activities of AST and ALT between the only alcohol-treated rats and the alcohol and experimental plants-treated rats were no significant difference. The results suggest that C. fructus and M alba have a hypoglycemic effect, and reduce liver damage by accelerating acetaldehyde metabolism in alcoholic rats, so the combined effect of C. fructus and M alba may be considered as an alternative remedy for hangovers, alcohol-induced overweight and alcohol-induced diabetes.

• Natural Product Sciences
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• 제18권2호
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• pp.83-88
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• 2012

In order to facilitate the quality control of the fruits of Crataegus pinnatifida, a simple, accurate and reliable HPLC method was developed for the simultaneous determination of the three bioactive compounds: chlorogenic acid (1), rutin (2), and hyperin (3), which were selected as the chemical markers of C. pinnatifida. Separation was achieved on an Agilent Eclipse XDB-C18 column with a gradient solvent system of 0.1% trifluoroacetic acid aqueous-acetonitrile at a flow-rate of 1.0 mL/min and detected at 254nm. All three calibration curves showed good linearity ($R^2$ > 0.998). The recoveries of three marker compounds were in the range of 94.87~111.52 %. The contents of chlorogenic acid (1), rutin (2), and hyperin (3) of the fruits of C. pinnatifida collected from 23 district markets in Korea, Japan, and China were 0.16~0.65 mg/g, 0.07~1.24 mg/g, and 0.03~0.62 mg/g, respectively. The results demonstrated that this method is simple and reliable for the quality control of the fruits of C. pinnatifida.

• 식물병연구
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• 제21권3호
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• pp.250-252
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• 2015

The tejocote (Crataegus spp.) is a tree considered to be native to Mexico. The aim of this study was to identify the causal agent of tejocote rust in the State of Puebla. Tejocote fruits were sampled in 2012 and 2013. The fungus was studied morphologically using light and scanning electron microscopy and molecularly using phylogenetic analysis of 18S and 28S rDNA genes. The fungus was identified as Gymnosporangium clavipes on tejocote fruits. To our knowledge, this is the first confirmed report of Gymnosporangium clavipes Cooke & Peck affecting Crataegus mexicana var. Chapeado and C. gracilior in Puebla Mexico.

• Archives of Pharmacal Research
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• 제23권2호
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• pp.155-158
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• 2000

Bioassay-guided fractionation of Crataegus pinnatifida (Rosaceae) gave two cytotoxic ursane-type triterpenes which were identified as uvaol (1) and ursolic acid (2) by physicochemical and spectroscopic methods. 3-Oxo-ursolic acid (3) was synthesized from ursolic acid (2) by Jones method. The cytotoxic activities of these compounds were tested against murine L1210 and human cancer cell lines (A549, SK-OV-3, SK-MEL-2, XF498, and HCT15) in vitro. Compounds 1 and 2 showed moderate cytotoxicities against L1210, whereas they showed weak activities against human cancer cell lines. However compound 3 exhibited potent cytotoxic activities both in murine and in human cancer cell lines.

• Journal of Applied Biological Chemistry
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• 제66권
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• pp.299-304
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• 2023

This study sought to evaluate the anticancer effects of Crataegus pinnatifida Bunge root extract (CPE) on murine Lewis lung carcinoma cells (LLC1) in vitro. CPE treatment (2.5, 5, 10 ㎍/mL, 24 h) of LLC cells led to a dose-dependent decrease in cell viability, while CPE treatment did not have a cytotoxic effect on non-cancer cells (NIH/3T3). CPE affects LLC by flipping the plasma membrane and making the membrane more permeable; by flow cytometry, CPE-induced annexin V and propidium iodide positivity, indicating induction of apoptosis in LLC cells. In addition, CPE enhanced the expression of apoptotic proteins caspase-3 and poly (ADP-ribose) polymerase 1 (PARP-1). CPE upregulated the proapoptotic protein BCL-2-associated X while downregulating the anti-apoptotic protein B-cell lymphoma 2 (BCL-2), suggesting that CPE induces apoptosis via the mitochondrial pathway. Furthermore, CPE upregulated the phosphorylation of the mitogen activated protein kinase p38. In conclusion, the results suggest that CPE has an anticancer effect in LLC cells by inducing apoptosis via p38.

• 동아시아식생활학회지
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• 제24권2호
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• pp.176-182
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• 2014

산사(Crataegus pinnatifida Bunge) 중의 생리활성물질이 이상지질혈증에 있어서 간 조직 및 간 기능 대사효소의 활성 변화 등에 생리적 효능이 있을 것으로 판단되어 Sprague Dawley계 수컷 흰쥐를 이용하여 실험을 수행하였다. 대조군인 NND군, 대조군에 산사 추출물을 섭취시킨 NNDH 군, 이상지질혈증군인 CDD 군, 이상지질혈증군에 산사 추출물을 섭취시킨 DDH 군으로 나누어 5주간 실험 사육하였다. ALP(alkaline phosphatase), LDH(lactate dehydrogenase), AST(aspartate aminotransferase) 및 ALT(alanine aminotransferase) 활성은 모두 동일한 경향을 나타내었다. NND 군과 NNDH 군 간에는 유의적인 차이가 없었다. 이상지질혈증군인 CDD 군이 가장 높은 활성을 보였고, 이상지질혈증군에 산사 추출물을 섭취시킨 DDH 군에서 감소하는 것으로 나타났다. 간장의 조직학적 검사는 NND 군과 NNDH 군에서는 어떠한 이상 소견도 발견하지 못하였으나, CDD 군에서 지방 세포의 수와 크기증가, 지방 변성 및 염증 세포 등이 관찰되었고, DDH 군에서 지방 세포의 수와 크기가 줄어들었으며, 지방 변성 등이 감소된 것을 관찰할 수 있었다. 따라서 산사 추출물은 지방세포 분화 억제 및 지방 분해 작용과 간 기능 개선 효과 있는 것으로 추정되며, 간 보호 기능성 물질로서 활용 가능성이 우수할 것으로 사료된다.

• Natural Product Sciences
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• 제19권4호
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• pp.329-336
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• 2013

The purpose of this study is to evaluate cytoprotective effect of Korean medicinal plants on alcohol-induced cytotoxicity in liver cells. Out of the 120 plant extracts tested in this study, 53 plant extracts enhanced alcohol-induced cytotoxicity in liver cells by 50~80%, while other 11 plant extracts including Crataegus pinnatifida reduced cytotoxicity by 1~68%. The results of DPPH free radical test and LDL lipid peroxidation test on the plant extracts that sharply reduced cytotoxicity in liver cells shows that Crataegus pinnatifida and Cinnamomum cassia had antioxidative effect. This study reports that the plant extracts that enhance or reduce ethanol-induced cytotoxicity in liver cells can be research objects as cytotoxic plants or cytotoxicity-protective plants.

• 동의신경정신과학회지
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• 제13권1호
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• pp.79-115
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• 2002

This research investigates the effect of the Crataegus pinnatifida BGE. var. major N.E. BR(CPVM) on Alzheimer's disease. Specifically, the effects of the DYHT extract on (1) $IL-1{\beta}$, IL-6, amyloid precursor proteins(APP), acetylcholinesterase(AChE), and glial fibrillary acidic protein(GFAP) mRNA of PC-12 cells treated with CTI05; (2) the AChE activity and the APP production of PC-12 cell treated with CT105; (3) the behavior; and (4) expression of $IL-1{\beta}$, $TNF-{\alpha}$, reactive oxygen species(ROS), nitrite oxide(NO); and (5) the infarction area of the hippocampus, and brain tissue injury in Alzheimer's diseased mice induced with CT105 were investigated. The results are as follow. 1. The CPVM extract suppressed the expression of $IL-1{\beta}$, IL-6, APP, AChE, and GFAP mRNA in PC-12 cells treated with CT105. 2. The CPVM extract suppressed the AChE activity and the production of APP significantly in PC-12 cells treated with CT105. 3. The CPVM extract group showed a significant inhibitory effect on the memory deficit for the mice with Alzheimer's disease induced by CT105 in the Morris water maze experiment. 4. The CPVM extract suppressed the over-expression of $IL-1{\beta}$, $TNF-{\alpha}$, ROS and NO in the mice with Alzheimer's disease induced by CT105. 5. The CPVM extract reduced the infarction area of hippocampus, and controlled the injury of brain tissue in the mice with Alzheimer's disease induced by CT105. These results suggest that the CPVM extract may be effective for the prevention and treatment of Alzheimer's disease.

• Journal of Ginseng Research
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• 제41권1호
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• pp.69-77
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• 2017

Background: Human skin undergoes distinct changes throughout the aging process, based on both intrinsic and extrinsic factors. In a process called photoaging, UVB irradiation leads to upregulation of matrix metalloproteinase-1, which then causes collagen degradation and premature aging. Mixtures of medicinal plants have traditionally been used as drugs in oriental medicine. Based on the previously reported antioxidant properties of Panax ginseng Meyer and Crataegus pinnatifida, we hypothesized that the mixture of P. ginseng Meyer and C. pinnatifida (GC) would have protective effects against skin aging. Methods: Anti-aging activity was examined both in human dermal fibroblasts under UVB irradiation by using Western blot analysis and in healthy human skin by examining noninvasive measurements. Results: In vitro studies showed that GC improved procollagen type I expression and diminished matrix metalloproteinase-1 secretion. Based on noninvasive measurements, skin roughness values, including total roughness (R1), maximum roughness (R2), smoothness depth and average roughness (R3), and global photodamage scores were improved by GC application. Moreover, GC ameliorated the high values of smoothness depth (R4), which means that GC reduced loss of skin moisture. Conclusion: These results suggest that GC can prevent aging by inhibiting wrinkle formation and increasing moisture in the human skin.

• 한방재활의학과학회지
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• 제29권4호
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• pp.15-27
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• 2019

Objectives This study was performed to evaluate anti-obesity effects of Crataegus pinnatifida (CP) on high-fat-diet induced obese mice. Methods The experimental animals were divided into four groups: normal diet (NOR) group, high fat diet (HFD) group, HFD+Xenical (XEN) group, and HFD+CP (CP) group. NOR group was fed a normal diet and the other three groups were fed high fat diet during the experiment. After the first two weeks of diet, XEN group and CP group were administered with XEN or CP for seven weeks, respectively. After that, we measured body weight, liver weight, fat weight, food intake, and serum concentrations of lipids and liver enzymes. Also the liver, intestine, fat tissue was removed to estimate the obesity-related mRNA expressions and the stool sample was collected to analyze the gut microbiota. Results We found that body weight, fat weight, and triglyceride level were decreased significantly in CP group compared to HFD group. Also CP significantly suppressed gene expressions associated with lipogenesis and inflammation, and increased gene expressions of browning of white adipose tissue and mitochondrial biogenesis. Moreover, it shifted the microbial diversity closer to that of NOR group and increased Firmicutes/Bacteriodetes ratio. Conclusions These results suggest that CP decrease body weight, fat weight and serum triglyceride. Also it inhibit inflammation and adipogenesis, altering gut microbial diversity and abundance. In conclusion, CP could be used as a therapeutic drug for obesity via gut microbiota modulation.