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INVESTIGATION OF EQUINE HEMATOLOGICAL CONSTITUENTS IN CENTRAL TAIWAN. I. DISTRIBUTION OF THE BLOOD CELL PARAMETERS AND THE BIOCHEMICAL COMPOSITIONS OF SERUM

  • Ju, J.C.;Cheng, S.P.;Fan, Y.K.;Hsu, J.C.;Chiang, S.K.;Chen, E.V.;Chang, S.H.;Chiou, S.C.
    • Asian-Australasian Journal of Animal Sciences
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    • v.6 no.1
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    • pp.147-153
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    • 1993
  • A total of 109 heads of horses and ponies from eight horseback riding clubs nearby Central Taiwan were investigated to evaluate the blood parameters and the biochemical compositions of serum for the documentation of clinical pathological diagnosis and exercise physiology. Blood samples were collected from the jugular vein of resting horses. The sex difference in the blood traits were compared both in horses and in ponies. Results shows that total plasma proteins (PP) and hematocrit (PCV) were found higher in male horses than in females (p < 0.05). The sexual effect also exertes a significant influence on the leucocyte (WBC) count, but not on the erythrocyte (RBC) concentration. According to the differential counts of leucocytes, the number of monocytes and lymphocytes was higher in the male pony than that of in male horse. A close relationship Between the erythrocyte sedimention rate (ESR) and the other blood parameters were found especially in PCV, RBC concentration, and plasma protein level. The average ESR observed at 60 minutes were $21.80{\pm}21.87mm$, $39.50{\pm}18.90mm$ and $43.73{\pm}17.89mm$ in stallions, geldings, and mares, respectively. Most of the biochemical components of horse serum detected were distributed in normal ranges, although some of the items show a great variation in such a large sample size.

Risk Factors of Syme Amputation in Patients with a Diabetic Foot (당뇨발 환자에서 Syme 절단술의 위험 인자)

  • Gwak, Heuichul;Kim, Jeongyo;Kim, Youngjun;Lee, Jeonghan;Lee, Woonseong
    • Journal of Korean Foot and Ankle Society
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    • v.20 no.2
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    • pp.78-83
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    • 2016
  • Purpose: This study examined the factors affecting the treatment of diabetes mellitus foot patients who had undergone a Syme amputation. Materials and Methods: This study included 17 patients diagnosed with a diabetes mellitus foot and who had undergone a Syme amputation from January 2010 to January 2014. Some of the risk factors (age, body mass index [BMI], disease duration, smoking, ankle brachial index [ABI], HbA1c, serum albumin, total lymphocyte, C-reactive protein [CRP], and serum creatine) that affect the successful Syme amputation were analyzed. Results: The healing rate of a Syme amputation was significantly higher when the lymphocyte count was above $1,500mm^3$ (p=0.029). The factors affecting the surgical outcome according to multivariate analysis were HbA1c and the BMI (p=0.014, p=0.013). Regarding reamputation, there was a significant difference with HbA1c, lymphocyte, and BMI (p=0.01, p=0.03, and p=0.01). No significant differences were observed with age, disease duration of diabetes mellitus, smoking, ABI, serum albumin, CRP, and serum creatine. Conclusion: The HbA1c level, BMI and total lymphocyte count are risk factors that must be considered for successful Syme amputation in patients with diabetic foot disease.

In the examination of PET/CT, Breast-tool production and availability of using FRP to check for breast disease. (양전자방출전산화단층촬영 검사에서 유방 질환 환자를 검사하기 위해 유리섬유강화플라스틱을 이용한 유방 틀의 제작 및 유용성)

  • Kim, Gab-Jung;Jeon, Min-Cheol;Han, Man-Seok;Seo, Sun-Youl;Kim, Nak-Sang;Bae, Won-Gyu
    • Journal of the Korea Convergence Society
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    • v.8 no.9
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    • pp.175-181
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    • 2017
  • The purpose of this study is to evaluate the breast tool to improve the diagnostic value of the image in the breast examination. Breast tool was made of using FRP. And then it was compared by radioactivity counting rate and image. In the evaluation of the Breast tool, the left and right counts per $1{\mu}Ci$ are 185 counts and 189 counts, respectively. The image obtained in the prone position was close to the circle. To increase diagnostic value of image, it is considered to use Breast-tool in the breast examination.

The Serum Level of Insulin Growth Factor-1 and Insulin Growth Factor Binding Protein-3 in Children with Henoch-Schönlein Purpura

  • Kim, Hee Jin;Jung, Su Jin;Lee, Jun Ho
    • Childhood Kidney Diseases
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    • v.20 no.1
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    • pp.23-28
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    • 2016
  • Purpose: We investigated whether serum levels of insulin growth factor-1 (IGF-1) and insulin growth factor binding protein-3 (IGFBP-3) are valuable in predicting clinical outcomes or are correlated with other laboratory findings in children with Henoch-$Sch{\ddot{o}}nlein$ purpura (HSP). Methods: We examined 27 children who were consecutively admitted to our hospital with HSP between January 2011 and February 2012. Blood tests (C-reactive protein, white blood cell count, platelet count, erythrocyte sedimentation rate, albumin, immunoglobulin A, complement C3, antineutrophil cytoplasmic antibody, IGF-1, IGFBP-3) and urine tests were performed upon admission. IGF-1 and IGFBP-3 were resampled in the recovery phase. Controls included 473 children whose IGF-1 and IGFBP-3 were sampled for evaluating their growth, at the outpatient department of pediatric endocrinology in our hospital. IGF-1 and IGFBP-3 were compared between the HSP children and controls, and between the acute and recovery phases in HSP children. The ability of these values to predict clinical outcomes including renal involvement was analyzed using bivariate logistic regression analysis (BLRA). Results: IGF-1 and IGFBP-3 were not different between the HSP children and controls ($148.7{\pm}117.6$ vs. $69.2{\pm}96.9$, P=0.290: $3465.9{\pm}1290.9$ vs. $3597.2{\pm}1,127.6$, P=0.560, respectively). There was no significant difference in IGF-1 or IGFBP-3 between acute and recovery phases. Based on the BLRA, no variable, including IGF-1 and IGFBP-3, could predict clinical outcomes including the presence of nephritis Conclusion: We concluded that IGF-1 and IGFBP-3 do not predict clinical outcomes of HSP, including renal involvement, in this study.

Changes of Growth and Hematological Constituents in the Rock Bream Oplegnathus fasciatus Exposed to TBT (TBT 노출에 따른 돌돔, Oplegnathus fasciatus의 성장 및 혈액성분의 변화)

  • Hwang, Un-Gi;Kim, Jun-Hwan;Kang, Ju Chan
    • Journal of fish pathology
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    • v.26 no.3
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    • pp.219-229
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    • 2013
  • Experiments were carried out to investigate the growth and hematological parameters of rock bream Oplegnathus fasciatus exposed to several different concentrations (0, 1, 2, 4, $8{\mu}g/L$) of tributyltin (TBT) for 4 weeks. Growth rate of the fish exposed to $8{\mu}g/L$ of TBT concentration was significantly lower than that of control group fish. The major hematological findings were significant decreases in the red blood cell count, hematocrit value and hemogobin concentration in the fish exposed to $8{\mu}g/L$. Although serum glucose concentration was significantly reduced compared to the control group, total protein concentration was found to be significantly increased over the control group. Exposure to $8{\mu}g/L$ of TBT concentration resulted in significant increase in the enzyme activities, such as glutamic oxalate transminase and glutamic pyruvate transminase in the fish. The present findings suggest that exposure to $8{\mu}g/L$ concentration of TBT can cause significant changes in growth and hematological parameters of rock bream Oplegnathus fasciatuso.

Effect of fractional ablative carbon dioxide laser with lidocaine spray on skin flap survival in rats

  • Choi, Manki;Park, Youngsoo;Kim, Yong-Ha;Chung, Kyu Jin
    • Archives of Craniofacial Surgery
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    • v.20 no.4
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    • pp.239-245
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    • 2019
  • Background: Lidocaine spray is a local anesthetic that improves random-pattern skin flap survival. The fractional ablative carbon dioxide laser (FxCL) produces vertical microchannels that delivers topically applied drugs to the skin. In this study, we hypothesized that FxCL therapy would enhance the lidocaine effect to improve random-pattern skin flap survival in rats. Methods: McFarlane random-pattern skin flaps were elevated in 48 rats, which were divided into four groups according to treatment: FxCL+lidocaine, FxCL, lidocaine, and nontreatment (control). On postoperative day 7, necrotic flap areas, the number of capillary vessels, and neutrophil count were evaluated. Anti-rat vascular endothelial growth factor (VEGF) and CD31 antibody activity were also evaluated by immunohistochemical staining. Results: Flap survival rate was $53.41%{\pm}5.43%$, $58.16%{\pm}4.80%$, $57.08%{\pm}5.91%$, and $69.08%{\pm}3.20%$ in the control, lidocaine, FxCL, and FxCL+lidocaine groups, respectively. Mean neutrophil count in the intermediate zone excluding the necrotic tissue was $41.70{\pm}8.40$, $35.43{\pm}6.41$, $37.23{\pm}7.15$, and $27.20{\pm}4.24cells/field$ in the control, lidocaine, FxCL, and FxCL+lidocaine groups, respectively. Anti-rat VEGF and CD31 antibody activity were the highest in the FxCL+lidocaine group. Conclusion: FxCL with lidocaine had a positive effect on random-pattern skin flap survival in rats. Thus, FxCL with lidocaine spray should be considered as a new treatment option to improve flap viability.

Effects of Omega-3 Fatty Acid on Endotoxin-induced Acute Lung Injury in Rabbits

  • Jang, Eun-A;Son, Sung-Kuk;Kang, Jeong-Hyeon;Lee, Seongheon;Kwak, Sang-Hyun
    • Biomedical Science Letters
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    • v.27 no.1
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    • pp.19-27
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    • 2021
  • This study was undertaken to clarify the effects of omega-3 fatty acid on endotoxin-induced acute lung injury. Rabbits were randomly assigned to one of four groups. Each group received intravenous infusion of saline only, saline and Escherichia coli endotoxin, omegaven infuison (0.5 mL/kg/hr) and endotoxin, lipoven (0.5 mL/kg/hr) and endotoxin respectively. Infusion of saline was started 0.5 hr before the infusion of saline or endotoxin, and omegaven and lipoven were started 2 hours after endotoxin infusion for 4 hours. The lungs of rabbits were ventilated with 40% oxygen. Mean blood pressure, heart rate, arterial oxygen tension (PaO2), and peripheral blood leukocyte were recorded. The wet/dry (W/D) weight ratio of lung and lung injury score were measured, and analysis of bronchoalveolar lavage fluid (BALF) was done. Endotoxin decreased PaO2, and peripheral blood leukocyte and platelet count. And it increased W/D ratio of lung, lung injury score and leukocyte count, percentage of PMN cells, concentration of IL-8 in BALF. Omegaven attenuated all these changes except for peripheral blood leukocyte counts. Omegaven attenuated endotoxin-induced acute lung injury in rabbits mainly by inhibiting neutrophil and IL-8 responses, which may play a central role in endotoxin-related lung injury.

Low level tritium analysis using liquid scintillation counter (액체섬광계수기를 이용한 저준위 트리튬 분석법연구)

  • Yoon, Yoon Yeol;Cho, Soo Young;Lee, Kil Yong;Kim, Yongje
    • Analytical Science and Technology
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    • v.20 no.5
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    • pp.419-423
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    • 2007
  • Environmental low level tritium analysis was studied using liquid scintillation counter(LSC) and electrolytic enrichment method. To obtain low level blank count, various counting vials were investigated. Among them, teflon coated PE vial had a lower blank count rate (1.86 cpm) and we obtained 2.01 Bq/L detection and tritium separation factor was above 20. LSC counting efficiency obtained $28.70{\pm}0.27%$ using the NIST tritium standard water sample.

A Study on the Technology Measuring Partial Discharge for Long Term Aging Experiments of Insulation Materials (장시간 절연체 열화실험을 위한 부분방전측정기술 연구)

  • Seon, Jong-Ho;Kim, Gwang-Hwa;Park, Jeong-Hu;Jo, Jeong-Su
    • The Transactions of the Korean Institute of Electrical Engineers C
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    • v.49 no.12
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    • pp.665-672
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    • 2000
  • This paper described the measurement technology to analyze the partial discharge characteristics for long term aging of insulations. This system was consisted of high voltage generation and measurement part, PD detection part, digital conversion and signal processing part. We used the VXI system for digital conversion and signal processing part. In the digital conversion part, we studied the error of partial discharge magnitude and memory capacity for reading digital signal with the sampling rate. In the signal processing part, we showed the program algorithm to count pulses and read peak values of partial discharge. The allowable minimum sampling rate of digizer was decided to 250kS/s through analyzing test. We confirmed that this system was very useful in the study of $\phi-q-n$ characteristics of long term PD experiments with specimens being consisted of internal void defects and CIGRE II electrodes.

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Effects of Estrogen on the Bacterial Uterine Diseases (세균성자궁질환(細菌性子宮疾患)에 있어서 Estrogen 이 미치는 영향(影響)에 관한 연구)

  • Oh, Soo Kak;Oak, Chong Wha
    • Korean Journal of Veterinary Research
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    • v.12 no.1
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    • pp.133-139
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    • 1972
  • Estrous and non-estrous rabbits were inoculated with E. coli or Streptococcus pyogenes, and the mixture of the two organisms, and bacterial count and histopathological studies of uterine horns were made to observe the effects of estrogen on the resistance of the uterus to bacterial infection. The results obtained were summarized as followings; 1. Four hours after inoculation of bactoria into uterine horn, the number of organisms was significantly lower in estrous rabbits than in non-estrous regardless of the kind of organisms inoculated. 2. The highest reduction rate of the organisms among the three bacterial inoculation groups was found in estrous rabbits inoculated with E. coli, and the lowest reduction rate was with Streptococcus pyogenes. 3. Histopathological changes of uterine horns induced five days after bacterial inoculation were observed. In estrous rabbits, a mild inflammatory reaction was found in Streptococcus pyogenes group, but a slight inflammatory reaction and only a negligible inflammatory reaction were observed in mixed bacteria group, and in E. coli group respectively. In non-estrous group, however, a marked inflammatory reaction was observed in Streptococcus pyogenes group, a moderate inflammatory reaction and a slight inflammatory reaction were observed in the mixed bacterial group and E. coli group, respectively.

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