• Title/Summary/Keyword: Cot

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앱시스산에 의해 기공이 닫히는 신호전달과정에서 G-단백질의 분할

  • 이영숙
    • Journal of Plant Biology
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    • v.37 no.4
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    • pp.429-434
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    • 1994
  • 식물 호르몬의 하나인 앱시스산이 식물의 기공을 닫게 하는 과정 중에 phospholipase C가 활성화되어 inositol 1,4,5-trisphosphate(P3)의 양이 증가함이 보고되었다 (Cot and Crain. 1994). 그러나 아직까지 공변세포에서 phospholipase C의 활성을 조절하는 G-단백질에 대한 보고는 없었다. 그러므로 앱시스산에 의한 기공닫힘과정에 G-단백질이 수반되는지를 조사하고자, G-단백질 활성의 저해제인 pertussis toxin과 촉진제인 cholera toxin을 처리하여 보았다. 닭의장풀(Commelina communis L.)의 잎 뒷면으로부터 얻은 온전한 표피층과 잠두(Vicia faba L)의 잎을 부분 분해하여 공변세포만을 남긴 표피층에 pertussis toxin을 처리하였을 때, 앱시스산에 의한 기공닫힘이 부분적으로 억제됨을 관찰하였다. 그러나 cholera toxin의 경우는 아무런 영향이 없었다. 공변세포만을 지닌 표피층에 pertussis toxin을 전처리한 후 앱시스산을 가했을 때, 앱시스산에 의한 IP3 양의 증가 양상이 억제됨을 확인하였다. 이러한 결과들로부터 앱시스산에 의한 기공닫힘과정에는 pertussis toxin-sensitive, phospholipase C-linked G-protein이 관여하고 있음을 알 수 있었다.

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Studies on the Physicochemical Properties of Korean Ginseng (Panax ginseng, C.M. Meyer) Root Starch 1. Starch Cantent and General Feachures (고려인삼 ( Panax ginseng C.A.Meyer)전분의 이화학적 특성에 관한 연구 제1보. 전분의 함량과 일반성상)

  • 김해중;조재선
    • Journal of Ginseng Research
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    • v.8 no.2
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    • pp.114-123
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    • 1984
  • This study was conducted to investigated the morphology and distribution of starch granule in ginseng root. The results obtained are as follows; The starch contents of main and lateral ginseng 1$.$cot were 23-32% and 14-16%, respectively, and it was varied significantly with growing season, namely 15-37% in Summer (May to August) and 3-6% in Winter (November to March). Thus the starch content of ginseng root was different depending on the portien of ginseng root and growing seasons, but a significant difference was not observed by a growing period of ginseng. The starch granules showed nearly round or oval shape having the diameter 2-8${\mu}$ and their size were increased with a growing period of ginseng. The crystalline structure of starch granules was found to be B-type by X-ray diffraction study.

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RNAi-Mediated Gene Silencing of Trcot1 Induces a Hyperbranching Phenotype in Trichoderma reesei

  • Gao, Fei;Li, Mengzhu;Liu, Weiquan;Bai, Yingguo;Tao, Tu;Wang, Yuan;Zhang, Jie;Luo, Huiying;Yao, Bin;Huang, Huoqing;Su, Xiaoyun
    • Journal of Microbiology and Biotechnology
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    • v.30 no.2
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    • pp.206-215
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    • 2020
  • Trichoderma reesei is the major filamentous fungus used to produce cellulase and there is huge interest in promoting its ability to produce higher titers of cellulase. Among the many factors affecting cellulase production in T. reesei, the mycelial phenotype is important but seldom studied. Herein, a close homolog of the Neurospora crassa COT1 kinase was discovered in T. reesei and designated TrCOT1, which is of 83.3% amino acid sequence identity. Functional disruption of Trcot1 in T. reesei by RNAi-mediated gene silencing resulted in retarded sporulation on potato dextrose agar and dwarfed colonies on minimal medium agar plates containing glucose, xylan, lactose, xylose, or glycerol as the sole carbon source. The representative mutant strain, SUS2/Trcot1i, also displayed reduced mycelia accumulation but hyperbranching in the MM glucose liquid medium, with hyphal growth unit length values decreased to 73.0 ㎛/tip compared to 239.8 ㎛/tip for the parent strain SUS2. The hyperbranching phenotype led to slightly but significantly increased cellulase secretion from 24 to 72 h in a batch culture. However, the cellulase production per unit of mycelial biomass was much more profoundly improved from 24 to 96 h.

A Study on the Effects of Lateral Displacement of Retaining Wall on the Distribution of Lateral Earth Pressure -In the Case of Sloping Noncohesive Backfills- (벽체(壁體)의 변위(變位)와 토압분포(土壓分布)와의 관계(關係)에 대(對)한 연구(硏究) -비점성토(非粘性土)의 지표면(地表面)이 경사(傾斜)질 경우-)

  • Cho, Hi-Doo
    • Journal of Korean Society of Forest Science
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    • v.17 no.1
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    • pp.29-34
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    • 1973
  • This study was performed for the purpose of determining the effects of distribution of the lateral earth pressure in the case of sloping backfills of being consisted of the idealized cohesionless fragmental masses. The displacements were classified as eight types by D_UBROVA (by patterns). B type among these has its turning point at the top of the wall, moves outwardly and is significant to gravitational structure because of its foundation elasticity which causes displacement. Therefore, it might be surely acknowledged that the resultant, follows; $$E=1/2{\cdot}rH^2\frac{sin(u-{\varepsilon})cos({\alpha}+{\varepsilon})}{cos(u+{\alpha})}{\cdot}cot(u+{\rho})(t/m^3)$$, is appropriate for applying it to the designing of the sand-catch dams. The results obtained are as follows: 1. Lateral earth pressure is proportional to the square of the wall heights. 2. The coefficient(K) is directly proportional to the sloping of backfill surface and inversely proportional to the displacement. 3. The distribution of the pressure looks like parabola, curve of second order (Fig. 5, b). 4. The distribution of the pressure strength looks like that of hydrostatic pressure (Fig. 5, c).

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On the Method of Applying COTS through Restriction Analysis in Weapon Systems Development (무기체계개발에서 제약사항 분석을 통한 상용부품(COTS) 적용 방안 연구)

  • Lee, Tae-Hyung;Lee, Jae-Chon
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.14 no.4
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    • pp.1896-1902
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    • 2013
  • As a concept that commercial parts are applied to weapon systems, Commercial Off-The-Shelf(COTS) was not well-received at a time when defense technology led commercial technology. However, the exponential growth of IT and the rapid expansion of commercial industry have made possible to make the cost low through mass production system within a short period, and the benefit of cost and schedule has been highlighted, triggering commercial parts to be applied to weapon systems. In order for commercial technology to be used in defense weapon systems, its performance in an extremely restricted condition of combat environment should be guaranteed. Generally, commercial parts have fundamental limitation in that the design and production requirements for commercial parts are different from those for the military. Therefore, it is required to confirm that commercial parts can be used for weapon systems through the approach of system engineering. This study presents the process tailored to characteristics of marine defense system and the method of analyzing its restricted condition in order to apply COTS to marine defense system. The study classifies the restricted conditions into general and special environments, suggests detailed process, and suggests useful tools to select appropriate items adapted to these restricted conditions in the commercial market. Lastly, as a case study for COTS applied to marine defense system, the study finds out the analysis results of application of COTS and restricted conditions of the warship electric system.

The Capacity of Multi-Valued Single Layer CoreNet(Neural Network) and Precalculation of its Weight Values (단층 코어넷 다단입력 인공신경망회로의 처리용량과 사전 무게값 계산에 관한 연구)

  • Park, Jong-Joon
    • Journal of IKEEE
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    • v.15 no.4
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    • pp.354-362
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    • 2011
  • One of the unsolved problems in Artificial Neural Networks is related to the capacity of a neural network. This paper presents a CoreNet which has a multi-leveled input and a multi-leveled output as a 2-layered artificial neural network. I have suggested an equation for calculating the capacity of the CoreNet, which has a p-leveled input and a q-leveled output, as $a_{p,q}=\frac{1}{2}p(p-1)q^2-\frac{1}{2}(p-2)(3p-1)q+(p-1)(p-2)$. With an odd value of p and an even value of q, (p-1)(p-2)(q-2)/2 needs to be subtracted further from the above equation. The simulation model 1(3)-1(6) has 3 levels of an input and 6 levels of an output with no hidden layer. The simulation result of this model gives, out of 216 possible functions, 80 convergences for the number of implementable function using the cot(x) input leveling method. I have also shown that, from the simulation result, the two diverged functions become implementable by precalculating the weight values. The simulation result and the precalculation of the weight values give the same result as the above equation in the total number of implementable functions.

Analysis of Cloud Properties Related to Yeongdong Heavy Snow Using the MODIS Cloud Product (MODIS 구름 산출물을 이용한 영동대설 관련 구름 특성의 분석)

  • Ahn, Bo-Young;Cho, Kuh-Hee;Lee, Jeong-Soon;Lee, Kyu-Tae;Kwon, Tae-Yong
    • Korean Journal of Remote Sensing
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    • v.23 no.2
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    • pp.71-87
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    • 2007
  • In this study, 14 heavy snow events in Yeongdong area which are local phenomena are analyzed using MODIS cloud products provided from NASA/GSFC. The clouds of Yeongdong area at observed at specific time by MODIS are classified into A, B, C Types, based on the characteristic of cloud properties: cloud top temperature, cloud optical thickness, Effective Particle Radius, and Cloud Particle Phase. The analysis of relations between cloud properties and precipitation amount for each cloud type show that there are statistically significant correlations between Cloud Optical Thickness and precipitation amount for both A and B type and also significant correlation is found between Cloud Top Temperature and precipitation amount for A type. However, for C type there is not any significant correlations between cloud properties and precipitation amount. A-type clouds are mainly lower stratus clouds with small-size droplet, which may be formed under the low level cold advection derived synoptically in the East sea. B-type clouds are developed cumuliform clouds, which are closely related to the low pressure center developing over the East sea. On the other hand, C-type clouds are likely multi-layer clouds, which make satellite observation difficult due to covering of high clouds over low level clouds directly related with Yeongdong heavy snow. It is, therefore, concluded that MODIS cloud products may be useful except the multi-layer clouds for understanding the mechanism of heavy snow and estimating the precipitation amount from satellite data in the case of Yeongdong heavy snow.

Cor triatriatum associated with partial anomalous pulmonary venous drainage: one case report (폐정맥 환류이상을 동반한 삼중방심 치험 1)

  • Kim, Yeong-Ho;Kim, Gong-Su
    • Journal of Chest Surgery
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    • v.17 no.3
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    • pp.381-388
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    • 1984
  • Cor triatriatum is rare congenital cardiac anomaly first described by Church in 1868. The anomaly consists of an abnormal partitioning of the left atrium by a fibromuscular membrane that divides the atrium into an upper chamber, which receives the pulmonary veins, and a lower chamber, which contains the atrial appendage and the mitral valve. The upper and lower chambers communicate through a stenotic fenestration in the membrane, which has the hemodynamic consequence of pulmonary venous obstruction. Recently we experienced cot triatriatum associated with partial anomalous pulmonary drainage to right atrium. The upper chamber was connected to right atrium through a sinus venous type of ASD and received left superior and both inferior pulmonary vein, whereas the lower chamber so called true left atrium communicated with right atrium through foramen ovale type of ASD, left atrial appendage and mitral orifice. And the anomalous membrane has no fenestrations which permit blood flow. The operation was made right atrial approach under the CPB. We excised completely the anomalous septum and reconstructed atrial septal defect with pericardial patch to drain the right upper pulmonary vein to the left atrium. The postoperative course has been good during follow up.

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A trend of Open rounded back /a:/ distribution in American English (미국영어에서의 후설원순저모음의 사용분포와 확산추이)

  • Park, Choong-Yon
    • Lingua Humanitatis
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    • v.8
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    • pp.313-329
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    • 2006
  • In some American English dialects, the vowels /a:/ or /c:/ have been replaced with /a:/, as in caught /ka:t/ dog /da:g/ that were pronounced in /kc:t/, and /dc:g/ previously. General American does not have /a:/ in its vowel system. But in East American and Western Pensylvania, cot and caught are homophones, /ka:t/, and similarly with other pairs such as collar vs. caller, stock vs. stalk, don vs. down, knotty vs. naughty. The use of /a/ or /c/ is quite unstable, and is a well-known diagnostic for distinguishing the northern speech area of the United States from the midland and southern area. For an increasing number of Americans, however, entirely lack the opposition between /a/ and /c/, merging to /a/, referring to 'both' of the vowels of lot. This paper investigates the use of /b:/ in American dialects and its relationship with /a:/, /c:/ in AE, and with short /a/ in RP. Examining the isoglosses of the use of /a:/ in various databases of the phonological atlas of North America, this paper discusses the use, position, and trend of merger to /a:/ from the vowels of /a:/ and /c:/ in Current American English.

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Cluster Analysis for Foot Type(I) - The subject of the college women between the age of 19~23 years - (발의 형태 분석을 위한 군집분석(I) - 19~23세 여자 대학생을 중심으로 -)

  • 문명옥
    • Journal of the Korean Society of Clothing and Textiles
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    • v.18 no.2
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    • pp.211-220
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    • 1994
  • The purpose of this study was to analyze the foot type by cluster analysis for footwear. The sample size for the study was 200 college womens between age 19 and 23 in Pusan urban area. There were measured 17 items of the foot for factor analysis and cluster analysis. The result was as follows : 1. 1'here were 9 items selected by factor analysis. 2.'rho cluster analysis of the foot must be analyzed by direct and indirect measurement indivisually. 3. The cluster analysis of the direct measurement ; Cluster 1 : 1'he foot length is all much the same to mean value of this age group and the items of width and circumference are relatively small to other clusters. Cluster 2 ; The foot length is relatively small to other clusters and the items of width and circumference are all much the same to mean value of this age group. Cluster 3 ; The foot sine Is relatively large to other clusters. 4. The cluster analysis of indirect measurement ; Cluster 1 ; The (cot print angle is high find Metatarso-Phalanx angle is transformed Cluster 2 ; The foot print angle is low and Melatarso-Phalanx angle is normal. Cluster 3 : Tho foot print angle Is middle and Metatarso-Phalanx angle is all the mush same to mean value of this age group. Cluster 4 . The foot print angle Is the most value and Metatarso-Phalanx angle is normal.

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