• 미생물학회지
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• 제37권1호
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• pp.28-36
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• 2001

본 연구에서는 세포주기의 Gl/S기에 관련된 유전자의 작용기작을 이해하기 위하여 출아효모인 Saccharomyces cerevisiae를 사용하여 세포주기 진행 및 조절에 관련된 변이주를 분 리하여 특성화하는 연구를 수행하였다. 먼저 새로운 변이주를 분리하기 위해서 HeLa 세포와 출아효모에서 Gl을 유발하는 CPO(ciclopirox olamine) 약제에 대해 감수성을 보이는 변이주를 선별하였다. 그 결과, 총 31개의 CPO에 대한 감수성 변이주들이 분리되었고, ciclopirox olamine sensitivity의 첫 글자를 따서 con mutants라 명명하였다. cos 변이주들의 표현형의 특성을 결정하기 위해 MMS(methylmethane sulfonate)와 HU(hydrsxyurea)에 대한 감수성을 조사하여 그 성질에 따라 4개의 group으로 나누었다. Group I에는 cos27, cos28, cos32, cos33, cos36, cos37, cos40, cos42, cos46, cos50, cos52, cos53 변이주가 포함되고, 이들은 MMS와 HU 두 약제 모두에 대해서 감수성을 보여 S기 checkpoint에 관련된 것으로 보이고, Group II는 cos43, cos48 변이주로 MMS에 대해서 감수성을 지녀 G1기 또는 G2기 checkpoint에 관련된 것으로 추측된다. Group III 변이주에는 cos35, cos47, cos54, cos55, cos56이 포함되고 HU에 대해서 감수성을 보여 S기에 관련된다고 보여지며, Group IV 변이 주는 cos29, cos30, cos31, cos34, cos38, cos39, cos41, cos44, cos45, cos49, cos51, cos57로서 단지 CPO에 대해서만 감수성을 나타냈다. 더욱이 변이주의 최종표현형을 형광현미경을 이용하여 조사하여, S기 checkpoint에 관련된 것으로 보이는 cos37 변이주를 확인하였다. 또한, 변이주와 야생주의 이형접합체인 이배체를 만들어 변이주의 유전학적 분석을 수행하였다.

• Journal of Microbiology and Biotechnology
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• 제11권2호
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• pp.281-286
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• 2001

Since the number of amino groups which are exposed by deacetylation of acetyl-D-glucosamine influences antimicrobial activity, a chitosan oligosaccharide (COS) derivative by N-conjugation of COS with asparagine, an amino acid with two amino groups, was synthesized and the antimicrobial effect on E. coli growth was compared with other COS derivatives which were N-conjugated with glycine, alanine, aspartic acid, cysteins, an methionine, and unmodified COS. The structure of asparagine N-conjugated COS (Asn-COS) derivative was identified by using a FT-IR, $^{13}C\;FT-NMR$, and an elemental analyzer. The antimicrobial activity of Asn-COS against E. coli growth was significantly improved as compared to the other COS derivatives as well as COS itself. This means that Asn-COS with two positive charges strongly interacts with the carboxyl negative charges on the bacteria cell wall. The results for Asn-COS were as follows: 100% bactericidal activity, 0.002% MIC, and no growth of E. coli during 3 days of culture time, suggesting that Asn-COS may be useful as a new antibiotic agent.

• Journal of Microbiology and Biotechnology
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• 제14권4호
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• pp.686-692
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• 2004

To develop the enzyme-linked immunosorbent assay (ELISA) for the analysis of N-acetylehitooligosaccharides (NACOS) and chitooligosaccharides (COS), specific antibodies (Abs) were produced, and their properties were compared. N-acetylehitohexaose (NACOS6), chitohexaose (COS6), and COS mixture (COSM) conjugated to bovine serum albumin (BSA) were used to immunize rabbits. By the use of specific Abs and NACOS6-horseradish peroxidase (HRP), COS6-HRP, and COSM-HRP conjugates, competitive direct ELISA (cdELISA) was developed. The detection limits of NACOS6 by the anti-NACOS6 Ab and COS6 by the anti-COS6 and the anti-COSM Abs in the cdELISAs were about 0.2, 2, ana 2 ng/ml (ppb), respectively. In the cdELISA, the anti-NACOS6 Ab was found to recognize NACOS3-NACOS6, but not N-acetyl-D-glucosamine (GlcNAc), NACOS2, and COSs; the anti-COS6 Ab recognized COS2-COS6 and COSM, but not glucosamine (GlcN) and NACOSs. The recognition pattern of the anti-COSM Ab was almost the same as that of the anti- COS6 Ab, except that the former recognized COS2 and COS3 slightly better than the latter.

• 복식문화연구
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• 제13권6호통권59호
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• pp.919-933
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• 2005

This study goes into the field of CosPlay that takes place in Korea, and directly observes the people who engage in CosPlays. Based on in-depth interviews, this research identified the reasons why the CosPlay aficionados participate in this practice and their cultural preferences. Ethnography methodology was used to understand the behavior of the cultural entities of CosPlays. Moreover, this research attempted to understand their daily formalities through their own perspective and language instead of superficial language. In general, CosPlay form that they manifest is classified into two main categories: those who enjoy posing by becoming their favorite characters and those like to wear the clothes of their favorite characters and go up on the stage to perform an act of animation or game where the applicable character appears. Some of these groups are: 1) 'Bishojo CosPlay' that CosPlays mostly Bishojo cartoon characters and wants to just stand out, 2) 'Aekyo' group that CosPlays merely for the sake of the enjoyment of wearing costumes instead of having the mania-like characteristics, 3) 'CosPlayer Group' who enjoy doing organized team CosPlay in order to present a performance, 4) 'J-Rock CosPlay' group that CosPlay Japanese visual rock groups and prepare to be among a professional CosPlay team, and 5) 'People related to Cospre.com' who try to make CosPlay rooted in as one of new cultures.

• 복식문화연구
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• 제14권1호
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• pp.1-15
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• 2006

This study examines the social meaning of the CosPlay, the growth potential of CosPlay culture and its effect on the related industry through the perspectives and language of the youths who enjoy CosPlay, based on the ethnographic research. Also, this study presents a comparative description of Korea and Japan CosPlay culture by the ethnographic methodology whose purpose is to define relationship of cause and effect with phenomenon. For further step, this study plans to emphasize the need to link culture, clothes and related industry in order to create a cultural environment where diversity co-exists. CosPlay is the mania culture of Japan that emulated the Halloween party of the West and that developed the party into a unique form. In Korea, this practice was accepted for the first time among a handful of youths, starting from the 1990s, after which, it was introduced to the masses while holding CosPlay related events. While CosPlay is succeeded as an industry in Japan, CosPlay in Korea is considered childish play due to the Korean culture of considering cartoon as a childish and low class genre which is enjoyed by youths. CosPlay in Korea faces the following changes: aging of the members who comprised the CosPlay culture at the initial stage; population increase, centered on middle and high school students; interest of the government and the businesses that wish to produce economic wealth by organizing CosPlay events into events for youths; and changes in the environment that comprised the surrounding of the CosPlay culture. CosPlay is an honest play that demonstrates one's effort on the stage through performance. Moreover, most of the middle and high school students who comprise the CosPlay culture demonstrate similar characteristics as mania type of people when it came to the reason that they enjoy CosPlay. However, they did not consider CosPlay culture as an important aspect of their lives. Instead, most of them said that they participate to relieve stress. Thus, they have the potential to move onto another form of youth culture that may appear more attractive to them. To them, it is not the CosPlay culture that is important, but the fact that CosPlay provides a forum where they can freely engage in play.

• 원예과학기술지
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• 제33권3호
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• pp.383-389
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• 2015

Chitooligosaccharide (COS), as antioxidant, extensively applied to food and juice preservation. In the present study, influences of COS on vase life and ornamental value of cut roses were investigated. Results showed that vase life of cut roses treated by COS was longer 6.4 days than one of control and ornamental character of cut roses was improved effectively by COS. The increase of vase life and ornamental value were chiefly governed by that COS improved water absorption capacity of cut roses. Besides that, COS decreased the contents of superoxide anion and hydrogen peroxide and lowered the levels of malondialdehyde in turn during the senescence process of cut roses. That was because that COS not only enhanced activities of antioxidant enzymes glutathione reductase, but also improved reduced glutathione contents in petals of cut rose. Therefore, COS could be used in commercial preservatives to improve the longevity of cut roses.

• 한국산학기술학회논문지
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• 제8권5호
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• pp.1062-1068
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• 2007

변압기 보호와 과전류를 막기 위한 목적으로 COS를 사용하고 있다. 그러나 COS 휴즈는 과전류가 흐르면 과부하에 의하여 용단되면서 강한 아크를 발생하여 공기 절연파괴를 일으킨다. 이러한 현상은 소음을 발생하면서 주변 거주자나 행인들에게 두려움을 준다. 그래서 실제 환경하에서 발생되는 소음을 시급히 정립할 필요가 있다. 본 논문은 COS 휴즈가 용단되면서 발생하는 충격성 소음의 특성을 파악하고 이러한 소음을 줄일 수 있는 방안을 제안한다.

• 미생물학회지
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• 제49권1호
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• pp.99-104
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• 2013

박테리오파지 P2 sir 변이체는 그것의 위성파지인 박테리오 파지 P4를 위해 비효율적인 도움파지로 알려졌다. 이러한 현상을 "P2 sir-관련 도움파지 비효율성"이라고 부르고 있으며, P2와 P4의 cos 지역에서의 DNA 염기배열 순서 차이가 이러한 현상을 나타나게 한다고 생각되었다. 이를 검증하기 위해, 여러 단계의 in vitro DNA 조작을 거쳐 P2의 cos 지역을 함유하는 유도체 P4인 P4 sid71 cosP2를 조성하였다. 일단계 생장 실험을 통해 그것의 후손 방출량을 결정하였다. 그 결과는, P4 sid71 cosP2에서 P4의 cos 지역을 P2의 cos 지역으로 대체한 것이 "P2 sir-관련 도움파지 비효율성"을 극복한 것으로 나타났다. P2 야생형과 P2 sir 돌연변이형을 도움파지로 삼아 준비한 P4 sid71 cosP2 파지 농축액들을 CsCl 부양 균등밀도 편차실험으로 분석하였다. 그 결과, 양 경우 모두 3개의 P4 유전체를 가진 파지 입자가 우세한 것으로 나타났다.

• 한국산학기술학회논문지
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• 제21권3호
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• pp.129-136
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• 2020

컷아웃스위치(COS: Cut Out Switch 이하 COS)는 전력계통에서 수용가로 전력을 송배전하기 위해 사용되는 변압기의 입력단에 설치되어 사고전류로부터 변압기를 보호하기 위해 설치되는 보호장치이다. COS는 크게 퓨즈링크와 COS몸체 및 접속부로 구성되어 사고전류시 퓨즈링크의 엘리먼트가 용단되어 사고전류를 차단하는 역할을 한다. COS 퓨즈링크가 용단되어 발생하는 강력한 아크가 화염과 소음을 유발시켜 주변지역 거주자에게 불쾌감 및 공포감을 주며, 아크화염으로 인하여 주변기기의 2차 피해를 유발시킬 수 있다. 본 논문에서는 COS 동작 시 발생되는 아크와 소음 및 보호협조의 문제점을 해결하기 위해 폭발형이 아닌 한류형 COS 퓨즈를 개발하였다. 또한 과전류 차단 기능이 없는 한류형 퓨즈의 단점을 개선하기 위해 퓨즈 엘리멘트, 스트라이커와 COS 퓨즈금구 개발을 통하여 과전류 차단성능의 신뢰성을 향상시켰다. COS의 동작 특성 향상은 퓨즈 엘리멘트의 최적 형상 도출, 스트라이커의 원활한 동작을 위한 동작선의 재질과 두께 및 저항 산정, 그리고 스트라이커와 연계된 하부금구류의 구조 개선을 통하여 수행하였다. 본 연구에서 개발한 COS 퓨즈링크는 공인기관의 시험을 통하여 차단성능과 보호협조 성능을 검증하였다. 시험은 본 연구의 한류형 COS와 기존의 폭발형 비한류형 COS의 비교 시험으로 수행하였다.

• 동아시아식생활학회지
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• 제13권2호
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• pp.122-129
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• 2003

This study was carried out in order to investigate the dextran formation and the internal structure of the chitosan-oligisaccharide(COS) Jeung-Pyun during the fermentation. To develop a functional Jeung-Pyun, COS was added to the rice at the level of 0, 2, 4, and 6% to the 2-hour-soaked rice. The pH, dextran and reducing sugar contents of COS batter, specific volume and the internal structure of COS were analyzed as a function of fermentation time. The specific volume of COS peaked at the 3 hour fermentation. The pH values of COS batters reduced with the fermentation time. The dextran content of the control batters peaked at the 7 hour fermentation. In contrast, the dextran contents of COS batters reduced up to 7th hour of fermentation, and then increased slowly until 20th hour of the fermentation process. From the air pore size and distribution of COS observed by SEM, the COS fermented fur 3 hour was judged as the best. It was concluded that COS prevented the fermation of dextran at the early stage of fermentation process.