• Korean Journal of Pharmacognosy
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• v.2 no.1
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• pp.41-42
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• 1971

Pale yellow microneedles$(m.p.\;228{\sim}230^{\cric})$ and colorless microneedles$(m.p.\;235{\sim}237^{\circ})$ were respectively obtained from the leaves of Cornus kousa $B_{UERGERI}$. These two substances were as iso-quercitrin and gallic acid by paper partition and thin-layer chromatographies and physicochemical tests.

• Journal of agriculture & life science
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• v.44 no.6
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• pp.79-82
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• 2010

Three types of leaf domatia; Pocket (P), Tufts (T) and Tufts-Pocket (TP) were observed in the five species of Cornus namely; Cornus kousa, C. controversa, C. officinalis, C. walteri and C. alba. In C. walteri no tufts were observed, the domatia was just a well developed pocket one. In C. officinalis and C. kousa, tufts domatia of brownish color were observed but C. officinalis developed denser tufts than those observed in C. kousa. Unlike in C. kousa where the domatia covered about only 1/2 of the axil, in the C. officinalis tufts covered almost 3/4 of the axil. In both species the tufts domatia were located in the inner side of the leaf axil. In C. controversa and C. alba whitish colored tufts were observed covering Pockets and hence the Tuft Pocket domatia. In C. controversa the tufts were more than in C. alba but in both species the tufts were short and were located in the inner sides of the axil. Thus, based on these physiological features it was concluded that C. officinalis and C. kousa are sister species likewise C. controversa and C. alba while C. walteri belong to another lineage. The advancement of domatia (absence of tufts) in C. walteri suggests that they are the most advanced while C. officinalis and C. kousa the most primitive due to their possession of highly developed tufts and C. controversa and C. alba the intermediates.

• Applied Biological Chemistry
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• v.49 no.1
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• pp.82-85
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• 2006

The fruits of Cornus kousa Burg. were extracted with 80% aqueous MeOH, and the concentrated extract was partitioned with EtOAc, n-BuOH and $H_2O$, successively. From the EtOAc fraction, three sterols were isolated through the repeated silica gel and ODS column chromatographies. According to the results of physico-chemical data including NMR, MS and IR, the chemical structures of the compounds were determined as ${\beta}-sitosterol$ (1), $stigmast-4-en-6{\beta}-ol-3-one$ (2) and daucosterol (3). They were the first to be isolated from Cornus kousa Burg.

• Journal of Environmental Science International
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• v.12 no.1
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• pp.15-22
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• 2003

Fifteen major naturally populated habitats around the Mt. Halla, Jeju province for wild Cornus kousa are mostly located on the southeast side of the mountains at 850-1,550m in altitude at sea level and are in half sunney areas. More than 40.0% of the naturally populated area of Cornus kousa are edge of forest areas and 53.3% populate on the steep slope of the mountain in 0-5℃. The direction of the slop for about 40.0% of total populated areas is southeast. In the naturally populated areas, total of 64 taxa dividing into 42 families, 59 genera 56 species and 8 varieties have been shown, mostly Hydrangea petiolaris, Rubus oldhamii, Ilex crenata, Sasa quelpaertensis, Ligustrum obtusifolium. It is suggested that these areas are secondary forest in a typical deciduous forest in Mt. Halla where the second transition had been progressed after forest damage or environmental stress. The index of species diversity of plant groups in these areas is 1.286-4.238 based on the Shannon-Wiener's method.

• Korean Journal of Plant Resources
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• v.19 no.4
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• pp.509-514
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• 2006

For the variant identification of Korean dogwood, Cornus kousa Buerg., we investigated useful genetic marker using the ISSR primer. The overall amplicons was 58 in six primers and the mean number of amplicons per primer was 9.67. The UPGMA dendrogram based on the genetic distance showed two major clusters composed of pink-bracted and white-bracted groups respectively. And white-bracted group was divided to two groups, island and inland groups. This result showed that ISSR analysis provides useful markers for the variant identification even in the seedling or young tree of Cornus kousa, especially pink-bracted individuals of Isl. Oenaro.

• Korean Journal of Medicinal Crop Science
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• v.10 no.5
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• pp.327-332
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• 2002

This study was conducted to investigate the immuno-regulatory effect and apoptosis of L1210 and HL60 leukemia cells of methanol-extracts of Cornus kousa Burg(CKB). The proliferation of mouse splenocytes and thymocytes enhanced by the addition of $10\;{\mu}g/ml$ of CKB. CKB were administered p.o. once a day for 7 days in adult male BALB/c mice. CKB increased the splenic and thymic T lymphocytes, especially the number of $T_H$ cells markedly increased by the treatment of CKB. CKB treatment induced the apoptotic cell death in L1210 mouse leukemia and HL60 human leukemia cells. In addition, CKB also accelerated the phagocytic activity in peritoneal macrophages and increased the production of plaque forming cells. These results suggest that CKB have an various immuno-regulatory property.

• Journal of Korean Society of Forest Science
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• v.95 no.3
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• pp.261-267
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• 2006

A nursery trial comprising 109 progenies of 5 populations of Cornus kousa was analysed using multivariate analysis of 12 quantitative traits (growth and leaf form). The aim of this study was to examine geographic variation of the species based on the single, existing nursery trial to support a genetic resource conservation plan of Cornus kousa in Korea. Nested Anova showed that there were statistically significant differences among populations as well as among families within populations in all 12 quantitative traits. In 10 of 12 traits, variance components among families within populations were higher than those among populations. Cluster analysis using complete linkage method showed three groups to Euclidean distance 0.8. Among principal components, primary 2 principal components appeared to be major variables because of the loading contribution of 91.9%. The first contribution component was maximum width, vein number, blade length/petiole length and upper 1/3 width lower 1/3 width; the second one was height, diameter at root collar, blade length, upper 1/3 width, petiole length and petiole length/vein number, respectively. But all characters showed no significant difference with the pattern of geographic distribution.

• Journal of the Korean Institute of Landscape Architecture
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• v.32 no.2
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• pp.120-129
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• 2004

This study was carried out to develop new varieties with early maturing and large fruit size in the Korean native dogwood (Cornus kousa) for woody landscape plants from native sites or cultivated area. The results are obtained as follows; We selected strains with early maturing and large fruit. size from the habitats from 2000 to 2003. ‘SKK 2-1’ had 4.0cm of fruit size while 2.0 cm of native variety. In addition, ‘SKK 2-2’, ‘SKK 2-3’, ‘SKK 2-4’, and ‘SKK 2-5’ with large fruit and early maturing were preliminary selected from the habitats. In the preliminary selections, ‘SKK 2-1’ was finally selected as new early maturing variety with large fruits in 2003. New early maturing variety with large fruit size, C. kousa var. ‘SKK 2-1’ was selected from 200 plants of 5-year-old seedlings in C. kousa in 5-12 Nung wean ri Goo sung myun Yong In, Kyung gi do, Korea in 2000. Their fruits were colorized in September 18, 20∼25 days earlier than October 7 of native varieties. Its fruit diameter is larger than 4cm. Its fruit weight was 5 times as heavy as native cultivars grown in Korea. This useful characters were still inherited after grafting. However, characteristics of growth, leaf, flower, and flowering period were not different from those of native varieties. Therefore, it was very promising landscape woody plant, having large fruits in early fall season. The rooting rate of Korean dogwood was high by the IBA treatment. The optimum date for softwood cutting was on June 25 ∼ July 25 when the rooting rate was more than 80%. In addition. Korean dogwood was easily propagated by grafting. Therefore, new early maturing variety with large fruits, C. kousa var. ‘SKK 2-1’ is easily propagated by softwood cutting or grafting and then is a promising cultivar as a woody landscape plant.

• Journal of Applied Biological Chemistry
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• v.50 no.3
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• pp.144-147
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• 2007

Dried, unripe fruits of Cornus kousa Burg. were extracted with 80% aqueous MeOH and the concentrated extracts were partitioned between EtOAc and $H_2O$. From the EtOAc fraction, four flavonoids were isolated through repeated silica gel, ODS and Sephadex LH-20 column chromatographies followed by a preparative HPLC. Based on the spectroscopic data including NMR, MS and IR, the chemical structures of the compounds were determined as kaempferol (1), astragalin (2), hyperin (3) and isoquercitrin (4). These compounds were isolated for the first time from the fruits of this plant.

• Proceedings of the Korean Society of Crop Science Conference
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• 2006.04a
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• pp.554-555
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• 2006