• Title/Summary/Keyword: Core composition

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In Vitro Formation of Protein Nanoparticle Using Recombinant Human Ferritin H and L Chains Produced from E. coli

  • RO HYEON SU;PARK HYUN KYU;KIM MIN GON;CHUNG BONG HYUN
    • Journal of Microbiology and Biotechnology
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    • v.15 no.2
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    • pp.254-258
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    • 2005
  • We have conducted in vitro reconstitution study of ferritin from its subunits FerH and FerL. For the reconstitution, FerH was produced from an expression vector construct in Escherichia coli and was purified from a heat treated cell extract by using one-step column chromatography. FerL was expressed as inclusion bodies. The denatured form of FerL was obtained by a simple washing step of the inclusion bodies with 3 M urea. The reconstitution experiment was conducted with various molar ratios of urea-denatured FerH and FerL to make the ferritin nanoparticle with a controlled composition of FerH and FerL. SDS-PAGE analysis of the reconstituted ferritins revealed that the reconstitution required the presence of more than 40 molar$\%$ of FerH in the reconstitution mixture. The assembly of the subunits into the ferritin nanoparticle was confmned by the presence of spherical particles with diameter of 10 nm by the atomic force microscopic image. Further analysis of the particles by using a transmission electron microscope revealed that the reconstituted particles exhibited different percentages of population with dense iron core. The reconstituted ferritin nanoparticles made with molar ratios of [FerH]/[FerL]=l00/0 and 60/40 showed that 80 to $90\%$ of the particles were apoferritin, devoid of iron core. On the contrary, all the particles formed with [FerH]/[FerL]=85/ 15 were found to contain the iron core. This suggests that although FerH can uptake iron, a minor portion of FerL, not exceeding $40\%$ at most, is required to deposit iron inside the particle.

RADIOLOGICAL CHARACTERISTICS OF DECOMMISSIONING WASTE FROM A CANDU REACTOR

  • Cho, Dong-Keun;Choi, Heui-Joo;Ahmed, Rizwan;Heo, Gyun-Young
    • Nuclear Engineering and Technology
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    • v.43 no.6
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    • pp.583-592
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    • 2011
  • The radiological characteristics for waste classification were assessed for neutron-activated decommissioning wastes from a CANDU reactor. The MCNP/ORIGEN2 code system was used for the source term analysis. The neutron flux and activation cross-section library for each structural component generated by MCNP simulation were used in the radionuclide buildup calculation in ORIGEN2. The specific activities of the relevant radionuclides in the activated metal waste were compared with the specified limits of the specific activities listed in the Korean standard and 10 CFR 61. The time-average full-core model of Wolsong Unit 1 was used as the neutron source for activation of in-core and ex-core structural components. The approximated levels of the neutron flux and cross-section, irradiated fuel composition, and a geometry simplification revealing good reliability in a previous study were used in the source term calculation as well. The results revealed the radioactivity, decay heat, hazard index, mass, and solid volume for the activated decommissioning waste to be $1.04{\times}10^{16}$ Bq, $2.09{\times}10^3$ W, $5.31{\times}10^{14}\;m^3$-water, $4.69{\times}10^5$ kg, and $7.38{\times}10^1\;m^3$, respectively. According to both Korean and US standards, the activated waste of the pressure tubes, calandria tubes, reactivity devices, and reactivity device supporters was greater than Class C, which should be disposed of in a deep geological disposal repository, whereas the side structural components were classified as low- and intermediate-level waste, which can be disposed of in a land disposal repository. Finally, this study confirmed that, regardless of the cooling time of the waste, 15% of the decommissioning waste cannot be disposed of in a land disposal repository. It is expected that the source terms and waste classification evaluated through this study can be widely used to establish a decommissioning/disposal strategy and fuel cycle analysis for CANDU reactors.

The Physical, Mechanical, and Sound Absorption Properties of Sandwich Particleboard (SPb)

  • ISWANTO, Apri Heri;HAKIM, Arif Rahman;AZHAR, Irawati;WIRJOSENTONO, Basuki;PRABUNINGRUM, Dita Sari
    • Journal of the Korean Wood Science and Technology
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    • v.48 no.1
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    • pp.32-40
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    • 2020
  • While the utilization of wood as a raw material in related industries has been increasing with the population increasing, the availability of wood from natural forests has continued to decline. An alternative to this situation is the manufacture of particleboard from non-wood lignocellulose materials through the modification of sandwich particleboard (SPb) using bamboo strands as reinforcement. In this study, strandsof belangke bamboo (Gigantochloa pruriens W) and tali bamboo (Gigantochloa apus) were utilized. The non-wood particles included sugar palm fibers, cornstalk, and sugarcane bagasse. The board was made in a three-layer composition of the face, back, and core in a ratio of 1: 2: 1. The binder used was 8% isocyanate resin. The sheet was pressed at a temperature of 160℃ for 5 min under a pressure of 3.0 N/㎟. Testing included physical and mechanical properties based on the JIS A 5908 (2003) standard, while acoustic testing was based on ISO 11654 (1997) standards. The results showed that using bamboo strands as reinforcement has an effect on the mechanical and physical properties of SPb. Almost all the types of boards met the JIS A 5908 (2003) standards, with the exception of thickness swelling (TS) and internal bond (IB) parameters. Based on the thickness swelling parameter, the C-type board exhibited the best properties. Overall, the B-type board thatused a belangke bamboo strand for the surface and sugarcane bagasse as the core underwent the best treatment. Based on the acoustical parameter, boards using a tali bamboo strand for the surface and sugar palm fiber as the core (E-type board) exhibited good sound absorption properties.

Magnetic Loss of Mn-Zn Ferrite Cores Used for SMPS (SMPS용 Mn-Zn 페라이트 코어의 자기손실 특성)

  • 권태석;김성수
    • Journal of the Korean Magnetics Society
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    • v.9 no.3
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    • pp.149-153
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    • 1999
  • Frequency and temperature dependence of magnetic loss has been investigated in Mn-Zn ferrites containing the sesistive temary compounds of $SiO_2-CaO-V_2O_5$. The Mn-Zn ferrite with the composition of $MnO:ZnO:Fe_2O_3=36:11:53$(by mol %) are prepared by self-propagating high-temperature synthesis. From the results of frequency dependence of core loss, it has been found that the hysteresis loss is dominant at low frequency and the eddy current loss becomes more dominant as the frequency increases. With the addition of resistive compound, the frequency dependence of core loss, it has been found that the hysteresis loss is dominant at low frequency and the eddy current loss becomes more dominant as the frequency increases. With the addition of resistive compound, the frequency region where the hysteresis loss is dominant becomes wide. The core-loss minimum occurs at about 4$0^{\circ}C$ in the specimens with the additive because of the reduction in eddy current loss.

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Development of Simplified DNBR Calculation Algorithm using Model-Based Systems Engineering Methodology

  • Awad, Ibrahim Fathy;Jung, Jae Cheon
    • Journal of the Korean Society of Systems Engineering
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    • v.14 no.2
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    • pp.24-32
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    • 2018
  • System Complexity one of the most common cause failure of the projects, it leads to a lack of understanding about the functions of the system. Hence, the model is developed for communication and furthermore modeling help analysis, design, and understanding of the system. On the other hand, the text-based specification is useful and easy to develop but is difficult to visualize the physical composition, structure, and behaviour or data exchange of the system. Therefore, it is necessary to transform system description into a diagram which clearly depicts the behaviour of the system as well as the interaction between components. According to the International Atomic Energy Agency (IAEA) Safety Glossary, The safety system is a system important to safety, provided to ensure the safe shutdown of the reactor or the residual heat removal from the reactor core, or to limit the consequences of anticipated operational occurrences and design basis accidents. Core Protection Calculator System (CPCS) in Advanced Power Reactor 1400 (APR 1400) Nuclear Power Plant is a safety critical system. CPCS was developed using systems engineering method focusing on Departure from Nuclear Boiling Ratio (DNBR) calculation. Due to the complexity of the system, many diagrams are needed to minimize the risk of ambiguities and lack of understanding. Using Model-Based Systems Engineering (MBSE) software for modeling the DNBR algorithm were used. These diagrams then serve as the baseline of the reverse engineering process and speeding up the development process. In addition, the use of MBSE ensures that any additional information obtained from auxiliary sources can then be input into the system model, ensuring data consistency.

Quantitative and Qualitative Considerations to Apply Methods for Identifying Content Relevance between Knowledge Into Managing Knowledge Service (지식 간 내용적 연관성 파악 기법의 지식 서비스 관리 접목을 위한 정량적/정성적 고려사항 검토)

  • Yoo, Keedong
    • The Journal of Society for e-Business Studies
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    • v.26 no.3
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    • pp.119-132
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    • 2021
  • Identification of associated knowledge based on content relevance is a fundamental functionality in managing service and security of core knowledge. This study compares the performance of methods to identify associated knowledge based on content relevance, i.e., the associated document network composition performance of keyword-based and word-embedding approach, to examine which method exhibits superior performance in terms of quantitative and qualitative perspectives. As a result, the keyword-based approach showed superior performance in core document identification and semantic information representation, while the word embedding approach showed superior performance in F1-Score and Accuracy, association intensity representation, and large-volume document processing. This study can be utilized for more realistic associated knowledge service management, reflecting the needs of companies and users.

Ecological impact of fast industrialization inferred from a sediment core in Seocheon, West Coast of Korean Peninsula

  • Choi, Rack Yeon;Kim, Heung-Tae;Yang, Ji-Woong;Kim, Jae Geun
    • Journal of Ecology and Environment
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    • v.44 no.4
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    • pp.212-221
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    • 2020
  • Background: Rapid industrialization has caused various impacts on nature, including heavy metal pollution. However, the impacts of industrialization vary depending on the types of industrializing activity and surrounding environment. South Korea is a proper region because the rapid socio-economical changes have been occurred since the late nineteenth century. Therefore, in this study, we estimate the anthropogenic impacts on an ecosystem from a sediment core of Yonghwasil-mot, an irrigation reservoir on the western coast of Korea, in terms of heavy metal concentrations, nutrient influx, and pollen composition. Results: The sediment accumulation rate (SAR) determined by 210Pb geochronology showed two abrupt peaks in the 1930s and 1950s, presumably because of smelting activity and the Korean War, respectively. The following gradual increase in SAR may reflect the urbanization of recent decades. The average concentrations of arsenic (As), copper (Cu), and lead (Pb) during the twentieth century were > 48% compared to those before the nineteenth century, supporting the influence of smelting activity. However, at the beginning of the twenty-first century, the As, Cu, and Pb concentrations decreased by 19% compared to levels in the twentieth century, which is coincident with the closure of the smelter in 1989 and government policy banning leaded gasoline since 1993. The pollen assemblage and nutrient input records exhibit changes in vegetation cover and water level of the reservoir corresponding to anthropogenic deforestation and reforestation, as well as to land-use alteration. Conclusions: Our results show that the rapid socio-economic development since the twentieth century clearly affected the vegetation cover, land use, and metal pollutions.

The Prediction Ability of Genomic Selection in the Wheat Core Collection

  • Yuna Kang;Changsoo Kim
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2022.10a
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    • pp.235-235
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    • 2022
  • Genome selection is a promising tool for plant and animal breeding, which uses genome-wide molecular marker data to capture large and small effect quantitative trait loci and predict the genetic value of selection candidates. Genomic selection has been shown previously to have higher prediction accuracies than conventional marker-assisted selection (MAS) for quantitative traits. In this study, the prediction accuracy of 10 agricultural traits in the wheat core group with 567 points was compared. We used a cross-validation approach to train and validate prediction accuracy to evaluate the effects of training population size and training model.As for the prediction accuracy according to the model, the prediction accuracy of 0.4 or more was evaluated except for the SVN model among the 6 models (GBLUP, LASSO, BayseA, RKHS, SVN, RF) used in most all traits. For traits such as days to heading and days to maturity, the prediction accuracy was very high, over 0.8. As for the prediction accuracy according to the training group, the prediction accuracy increased as the number of training groups increased in all traits. It was confirmed that the prediction accuracy was different in the training population according to the genetic composition regardless of the number. All training models were verified through 5-fold cross-validation. To verify the prediction ability of the training population of the wheat core collection, we compared the actual phenotype and genomic estimated breeding value using 35 breeding population. In fact, out of 10 individuals with the fastest days to heading, 5 individuals were selected through genomic selection, and 6 individuals were selected through genomic selection out of the 10 individuals with the slowest days to heading. Therefore, we confirmed the possibility of selecting individuals according to traits with only the genotype for a shorter period of time through genomic selection.

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Provenance of the ARA07C-St02B Core Sediment from the East Siberian Margin (동시베리아해 연변부 ARA07C-St02B 코어 퇴적물의 기원지 연구)

  • Koo, Hyo Jin;Lim, Gi Taek;Cho, Hyen Goo
    • Korean Journal of Mineralogy and Petrology
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    • v.35 no.1
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    • pp.13-24
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    • 2022
  • The Arctic Ocean is very sensitive to global warming and Arctic Ocean sediments provide a records of terrestrial climate change, analyzing their composition helps clarify global warming. The gravity core sediment ARA07C-St02B was collected at the East Siberian margin during an Arctic expedition in 2016 on the Korean ice-breaking vessel ARAON, and its provenance was estimated through sedimentological, mineralogical and geochemical analysis. The core sediment was divided into four units based on sediment color, sand content and ice-rafted debris content. Units 1 and 3 had higher sand and ice-rafted debris contents than units 2 and 4, and contained a brown layer, whereas units 2 and 4 were mainly composed of a gray layer. Correlation analysis using the adjacent core sediment ARA03B-27 suggested that the sediment units were deposited during marine isotope stage 1 to 4. The bulk mineral, clay mineral, and geochemical compositions of units including a brown layer differed from units including a gray layer. Bulk and clay mineral compositions indicated that coarse and fine sediments had a different origin. Coarse sediments might have been deposited mostly by the East Siberian Coastal Current from the Laptev Sea and the East Siberian Sea or by the Beaufort Gyre from the Chukchi Sea, whereas fine sediments might have been transpoted mostly by currents from the East Siberian Sea, the Chukchi Sea and the Beaufort Sea. Some of the coarse sediments in unit 1 and fine sediments in unit 3 might have been deposited by iceberg ice, sea ice or current from the Beaufort Sea and the Canada Archipelago. Investigating the geochemical composition of the potential origins will elucidate the origin and transportation of the study area's core sediments.

A Study on the Integrated Hybrid Spatial Model for Knowledge Information Institutions - Focused on the User Space of Public Library - (지식정보시설의 통합형 하이브리드 공간 모형 연구 - 공공도서관 이용자 공간을 중심으로 -)

  • Hwang, Mee-Young
    • Korean Institute of Interior Design Journal
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    • v.24 no.3
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    • pp.146-155
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    • 2015
  • It is an undeniable fact that the modern people's interest in knowledge and information and their roles are some of the core factors used to define the modern society. Besides, as phenomena indicating and leading the modern society, we have fusion, hybrid and convergence, etc. that combine several different elements into a new one. Such changes in social, cultural and technological circumstances require a paradigm shift for the composition of knowledge and information facilities and space. However, more diversified and advanced recently, interfaces for the delivery of knowledge and information cause users to show new behaviors and even change the spatial concept with space composition elements hybridized. Especially for the space of libraries, it is necessary to access and utilize any forms of knowledge and information data (digital + analog) regardless of time and space limitations and approach with an integrated spatial concept likely to change flexibly depending on social demands. In this light, this study aims to make a basic proposal for sustainable integrated-type hybrid space for the space of a public library in this era of knowledge and information. Particularly, this study intends to seek a way to establish a hybrid space model that can effectively converge the most important factors of the space of libraries (human-space(network)-information). With 18 different cases, this study analyzed knowledge and information composition systems, knowledge and information delivery systems and users' space systems. As a result, this study extracted 3 kinds of models, an information-based model, a culture-based model and an education-based model, with the integrated hybrid space model of knowledge and information facilities