• Title/Summary/Keyword: Core Sequence

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Mechanical behavior of outer square inner circular concrete-filled dual steel tubular stub columns

  • Ding, Fa-xing;Wang, Wenjun;Liu, Xue-mei;Wang, Liping;Sun, Yi
    • Steel and Composite Structures
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    • v.38 no.3
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    • pp.305-317
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    • 2021
  • The mechanical behavior of the outer square inner circular concrete-filled dual steel tubular (SCCFT) stub columns under axial compression is investigated by means of experimental research, numerical analysis and theoretical investigation. Parameters such as diameter ratio, concrete strength and steel ratio were discussed to identify their influence on the mechanical properties of SCCFT short columns on the basis of the experimental investigation of seven SCCFT short columns. By establishing a finite element model, nonlinear analysis was performed to discuss the longitudinal and transverse stress of the dual steel tubes. The longitudinal stress characteristics of the core and sandwich concrete were also analyzed. Furthermore, the failure sequence was illustrated and the reasonable cross-section composition of SCCFT stub column was proposed. A formula to predict the axial load capacity of SCCFT stub column was advanced and verified by the results from experiment and the finite element.

Multi-task learning with contextual hierarchical attention for Korean coreference resolution

  • Cheoneum Park
    • ETRI Journal
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    • v.45 no.1
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    • pp.93-104
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    • 2023
  • Coreference resolution is a task in discourse analysis that links several headwords used in any document object. We suggest pointer networks-based coreference resolution for Korean using multi-task learning (MTL) with an attention mechanism for a hierarchical structure. As Korean is a head-final language, the head can easily be found. Our model learns the distribution by referring to the same entity position and utilizes a pointer network to conduct coreference resolution depending on the input headword. As the input is a document, the input sequence is very long. Thus, the core idea is to learn the word- and sentence-level distributions in parallel with MTL, while using a shared representation to address the long sequence problem. The suggested technique is used to generate word representations for Korean based on contextual information using pre-trained language models for Korean. In the same experimental conditions, our model performed roughly 1.8% better on CoNLL F1 than previous research without hierarchical structure.

Whole-Genome Sequence of Priestia aryabhattai Strain S2 Isolated from the Rhizosphere of Soybean (Glycine max)

  • Amani Sliti;Min-Ji Kim;GyuDae Lee;Yeong-Jun Park;Jae-Ho Shin
    • Microbiology and Biotechnology Letters
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    • v.51 no.3
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    • pp.296-299
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    • 2023
  • We present the complete genome sequence of Priestia aryabhattai strain S2 isolated from the soybean rhizosphere. The genome consists of a single circular chromosome of 5,070,860 bp with a G+C content of 38.3% and 2 plasmids, P1(148,124 bp, GC content 33.3%) and P2 (76,418 bp, GC content 36.5%).

Analysis of Genotype and Flanking Sequence Tagged from pooled Ds Insertional lines in rice (벼 Ds 삽입변이 pooling 계통들의 FST 및 유전자형 분석)

  • Ahn, Byung-Ohg;Kim, Jeong-Ho;Ji, Sang-hye;Yun, Doh-Won;Park, Yong-Hwan;Ji, Hyeon-So;Eun, Moo-Young;Lee, Gi-hwan;Suh, Seok-Cheol;Lee, Myung-Chul
    • Korean Journal of Breeding Science
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    • v.40 no.4
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    • pp.387-393
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    • 2008
  • Over 7 individual rice (Oryza sativa L.) plants per a line were sowed and sampled by pooled sampling method for genomic DNA extraction. The 5,400 flanking sequence tags (FSTs) were analysed by adaptor PCR and direct sequencing. FST analysis showed that the intragenic FSTs, the intergenic FSTs, and the original insertional sequences including hot spot covered 48.1% (2,597), 25.6% (1,383), and 25% (1,350), respectively. The 2,597 intragenic FSTs were used for genotyping to determine whether they are heterozygous or homozygous, and 1,393 core lines were selected. Among them, 422 knockout genes were distributed on chromosome 3, while 56 - 157 intragenic FSTs scattered on other chromosomes. Among 1,393 FSTs, known genes such as transcription factor covered 59.4% (827), while unknown genes such as expressed protein covered 40.6% (566). RT-PCR indicated that some core lines had no expression or decreased expression level in their knockout genes. It means that core lines are very useful knockout lines for functional genomic studies.

Protein Ontology: Semantic Data Integration in Proteomics

  • Sidhu, Amandeep S.;Dillon, Tharam S.;Chang, Elizabeth;Sidhu, Baldev S.
    • Proceedings of the Korean Society for Bioinformatics Conference
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    • 2005.09a
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    • pp.388-391
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    • 2005
  • The Protein Structural and Functional Conservation need a common language for data definition. With the help of common language provided by Protein Ontology the high level of sequence and functional conservation can be extended to all organisms with the likelihood that proteins that carry out core biological processes will again be probable orthologues. The structural and functional conservation in these proteins presents both opportunities and challenges. The main opportunity lies in the possibility of automated transfer of protein data annotations from experimentally traceable model organisms to a less traceable organism based on protein sequence similarity. Such information can be used to improve human health or agriculture. The challenge lies in using a common language to transfer protein data annotations among different species of organisms. First step in achieving this huge challenge is producing a structured, precisely defined common vocabulary using Protein Ontology. The Protein Ontology described in this paper covers the sequence, structure and biological roles of Protein Complexes in any organism.

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cDNA Cloning and Developmental Expression of Hemolin in Bombyx mandarina

  • Kang Min Uk;Kim Kyung-A;Lee Jin Sung;Kim Nam Soon;Kang Seok-Woo;Nho Si-kab
    • International Journal of Industrial Entomology and Biomaterials
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    • v.10 no.2
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    • pp.101-106
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    • 2005
  • In this study, we describe the Bombyx mandarina hemolin cDNA. A sequence analysis of cDNA revealed a single open reading frame (ORF) of 1233 nucleotides. The deduced 410 amino acid sequence of B. mandarina hemolin contains 4 imunoglobulin (Ig) C-2 type domains. B. mandarina hemolin cDNA showed the highest sequence homology to known those of B. mori. The developmental profile in terms of expression level of hemolin mRNA was determined in the absence of a bacterial challenge. Hemolin mRNA was detected only in mid-gut, but not in hemocytes, fat body, testis, and silkglands. Hemolin mRNA in mid-gut was not detected until the spinning stage of the last instar larva, however, lit dramatically increased at the beginning of spinning and gradually decreased until pupal stage.

Comparison of event tree/fault tree and convolution approaches in calculating station blackout risk in a nuclear power plant

  • Man Cheol Kim
    • Nuclear Engineering and Technology
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    • v.56 no.1
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    • pp.141-146
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    • 2024
  • Station blackout (SBO) risk is one of the most significant contributors to nuclear power plant risk. In this paper, the sequence probability formulas derived by the convolution approach are compared with those derived by the conventional event tree/fault tree (ET/FT) approach for the SBO situation in which emergency diesel generators fail to start. The comparison identifies what makes the ET/FT approach more conservative and raises the issue regarding the mission time of a turbine-driven auxiliary feedwater pump (TDP), which suggests a possible modeling improvement in the ET/FT approach. Monte Carlo simulations with up-to-date component reliability data validate the convolution approach. The sequence probability of an alternative alternating current diesel generator (AAC DG) failing to start and the TDP failing to operate owing to battery depletion contributes most to the SBO risk. The probability overestimation of the scenario in which the AAC DG fails to run and the TDP fails to operate owing to battery depletion contributes most to the SBO risk overestimation determined by the ET/FT approach. The modification of the TDP mission time renders the sequence probabilities determined by the ET/FT approach more consistent with those determined by the convolution approach.

Sequence-specific interaction between ABD-B homeodomain and castor gene in Drosophila

  • Kim, Keon-Hee;Yoo, Siuk
    • BMB Reports
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    • v.47 no.2
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    • pp.92-97
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    • 2014
  • We have examined the effect of bithorax complex genes on the expression of castor gene. During the embryonic stages 12-15, both Ultrabithorax and abdominal-A regulated the castor gene expression negatively, whereas Abdominal-B showed a positive correlation with the castor gene expression according to real-time PCR. To investigate whether ABD-B protein directly interacts with the castor gene, electrophoretic mobility shift assays were performed using the recombinant ABD-B homeodomain and oligonucleotides, which are located within the region 10 kb upstream of the castor gene. The results show that ABD-B protein directly binds to the castor gene specifically. ABD-B binds more strongly to oligonucleotides containing two 5'-TTAT-3' canonical core motifs than the probe containing the 5'-TTAC-3' motif. In addition, the sequences flanking the core motif are also involved in the protein-DNA interaction. The results demonstrate the importance of HD for direct binding to target sequences to regulate the expression level of the target genes.

Genetically Independent Tetranucleotide to Hexanucleotide Core Motif SSR Markers for Identifying Lentinula edodes Cultivars

  • Saito, Teruaki;Sakuta, Genki;Kobayashi, Hitoshi;Ouchi, Kenji;Inatomi, Satoshi
    • Mycobiology
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    • v.47 no.4
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    • pp.466-472
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    • 2019
  • For the purpose of protecting the rights of Lentinula edodes breeders, we developed a new simple sequence repeat (SSR) marker set consisting only of genetically independent tetranucleotide or longer core motifs. Using available genome sequences for five L. edodes strains, we designed primers for 13 SSR markers that amplified polymorphic sequences in 20 L. edodes cultivars. We evaluated the independence of every possible marker pair based on genotype data. Consequently, eight genetically independent markers were selected. The polymorphic information content values of the markers ranged from 0.269 to 0.764, with an average of 0.409. The markers could distinguish among 20 L. edodes cultivars and produced highly repeatable and reproducible results. The markers developed in this study will enable the precise identification of L. edodes cultivars, and may be useful for protecting breeders' rights.