• Journal of Korean Academy of Nursing
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• v.36 no.1
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• pp.25-36
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• 2006

Purpose: The purpose of the study was to identify the adaptation process to problem based learning(PBL) among nursing students who have experienced PBL classes for two years. Method: Data was collected from 11 nursing students with in-depth interviews and direct observation of their PBL experiences by a researcher who has been a facilitator for PBL class for 3years. Immediately after the interviews all of them were transcribed. It was analyzed by the Ground theory of Corbin and Strauss. Results: A derived core category was 'Acquiring PBL'. 4 stages of the acquiring process were derived and written in time sequence: chaos, confusion, beginning insight, and achievement stage. Conclusion: The results will not only expand understanding of the students for the facilitator and school which has adopted PBL but also provide information to develop an orientation program for PBL. Further research on the facilitator's role experiences is recommended.

• 한국생물공학회:학술대회논문집
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• 2000.04a
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• pp.592-595
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• 2000

In human chromosome, a short sequence of DNA has been repeated a number of times. These repeats are called variable number of tandem repeat(VNTR) or short tandem repeat(STR) which has short repeat core. VNTR and STR are used in the field of forensic science, evolution, and anthropology. In this work, we examined allele frequencies of 3 VNTR(YNZ22, NeuR, D21S11) and one STR(Humth01) in a Korean population sample by polymerase chain reaction(PCR) followed by high-resolution polyacrylamide gelelectrophoresis(PAGE) with silver staining. Subsequently, the polymorphism information content(PIC) was calculated : the highest PIC was observed for the NeuR locus(0.95680) and lowest for the Humth01 locus(0.75809).

• Journal of Microbiology and Biotechnology
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• v.18 no.6
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• pp.1050-1058
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• 2008

A gene encoding a dextransucrase (dsrBCB4) that synthesizes only ${\alpha}$-1,6-linked dextran was cloned from Leuconostoc mesenteroides B-1299CB4. The coding region consisted of an open reading frame (ORF) of 4,395 bp that coded a 1,465-amino-acids protein with a molecular mass of 163,581 Da. The expressed recombinant DSRBCB4 (rDSRBCB4) synthesized oligosaccharides in the presence of maltose or isomaltose as an acceptor, plus the products included ${\alpha}$-1,6-linked glucosyl residues in addition to the maltosyl or isomaltosyl residue. Alignments of the amino acid sequence of DSRBCB4 with glucansucrases from Streptococcus and Leuconostoc identified conserved amino acid residues in the catalytic core that are critical for enzyme activity. The mutants D530N, E568Q, and D641N displayed a 98- to 10,000-fold reduction of total enzyme activity.

• Proceedings of the KSRS Conference
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• v.2
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• pp.812-815
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• 2006

Proteins are essential agents for controlling, effecting and modulating cellular functions, and proteins with similar sequences have diverged from a common ancestral gene, and have similar structures and functions. Function prediction of unknown proteins remains one of the most challenging problems in bioinformatics. Recently, various computational approaches have been developed for identification of short sequences that are conserved within a family of closely related protein sequence. Protein function is often correlated with highly conserved motifs. Motif is the smallest unit of protein structure and function, and intends to make core part among protein structural and functional components. Therefore, prediction methods using data mining or machine learning have been developed. In this paper, we describe an approach for protein function prediction of motif-based models using data mining. Our work consists of three phrases. We make training and test data set and construct classifier using a training set. Also, through experiments, we evaluate our classifier with other classifiers in point of the accuracy of resulting classification.

• Journal of the Korean Operations Research and Management Science Society
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• v.6 no.1
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• pp.65-70
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• 1981

L.G. Khachiyan's algorithm for solving a system of strict (or open) linear inequalities with integral coefficients is described. This algorithm is based on the construction of a sequence of ellipsoids in R$^n$ of decreasing n-dimensional volume and contain-ing feasible region. The running time of the algorithm is polynomial in the number of bits of computer core memory required to store the coefficients. It can be applied to solve linear programming problems in polynomially bounded time by the duality theorem of the linear programming problem. But it is difficult to use in solving practical problems. Because it requires the computation of a square roots, besides other arithmatic operations, it is impossible to do these computations exactly with absolute precision.

• Proceedings of the Korea Concrete Institute Conference
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• 2009.05a
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• pp.167-168
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• 2009

The CFT structure is applied to newly developed CFTA girder because of improvement of ductility deformation, stiffness and internal force of structure owing to the interaction between steel tube and core concrete. CFTA girder is the structure which can reduce tensile stress due to external loads by using its arch shape and prestress force. This paper proposed constructional stage procedure and represented analytical technique considering constructional stage to investigate the safety against bridge collapse on construction and on operation.

• Journal of Korean Academy of Nursing
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• v.34 no.8
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• pp.1416-1426
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• 2004

Purpose. The purpose of this study is to identify the role adaptation process experienced by head nurses. Methods. Data were collected from 10 head nurses with in-depth interviews about their actual experiences. All the interviews were recorded and transcribed. The collected data were analyzed by the grounded theory methodology of Strauss and Corbin. Results. The core category was identified as 'weighing and balancing,' and the role adaptation process was divided into six stages by time sequence: inquiring, approaching to others, bringing people into their fold, working with conviction, avoiding conflicts, and settling. Conclusion. The results of this study yield useful information for top mangers of nursing to identify, by stages, the demands of the head nurses in their role adaptation process. The findings of this study contributes to developing programs that facilitates the head nurses' role adaptation.

• Biomedical Science Letters
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• v.10 no.4
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• pp.523-528
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• 2004

The core factor of the study is integrated environment based PC-Cluster system and high speed access rate up to 155 Mbps, continuous collection system for bioinformatics information at home and abroad. The results of the study are establishment and stabilization of information and communication infrastructure, establishment and stabilization of high performance computer network up to 155 Mbps, development of PC-Cluster system with 32 nodes, a parallelized BLAST on Cluster system, which can provides scalable speedup in terms of response time, and development of collection and search system for bioinformatics information.

• Biotechnology and Bioprocess Engineering:BBE
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• v.5 no.3
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• pp.169-173
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• 2000

On human chromoscomes, a short sequence of DNA is known to repeat a number of times. These are called variable number of tandem repeat (VNTR) or short tandem respeat (STR) which has a short core. VNTR and STR are used in the filed of forensic science, evolution, and anthropology. In this work, we examined allele frequencies of one VNTR (YNZ22) and three STRs (NeuR, D21S11, Humth01) in a korean population sample by polymerase chain reaction (RCP) followed by high-resolution polyacrylamide gel electro-phoresis (PAGE) with silver stain. Subsequently, the polymorphism information content (PIC) was calculated : the hifhest PIC was observed in the NeuR locus (0.95680) and lowest in the Humth01 locus (0.75809).

• Genomics & Informatics
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• v.13 no.4
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• pp.152-155
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• 2015

Recently, the Encyclopedia of DNA Elements (ENCODE) Analysis Working Group converted data from ChIP-seq analyses from the Broad Histone track into 15 corresponding chromatic maps that label sequences with different kinds of histone modifications in promoter regions. Here, we publish a frequency profile of the three ChromHMM promoter states, at 200-bp intervals, with particular reference to the existence of sequence patterns of promoter elements, GC-richness, and transcription starting sites. Through detailed and diligent analysis of promoter regions, researchers will be able to uncover new and significant information about transcription initiation and gene function.