• Asian-Australasian Journal of Animal Sciences
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• v.13 no.5
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• pp.587-594
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• 2000

At present embryonic stem (ES) cells with confirmed pluripotential properties are only available in the mouse. Recently, we were able to isolate, culture and genetically transform primordial germ cell (PGC)-derived cells from pig embryos and demonstrate their ability to contribute to chimera development in the pig. In order to determine whether the system we developed could be used to isolate embryonic germ (EG) cells from other mammalian species, we placed isolated PGCs from cattle, goats, rabbits and rats in culture. Briefly, PGCs were isolated from fetuses of cow (day 30-50), goat (day 25), rabbit (day 15-18) and rat (day 11-12), and plated on STO feeder cells in Dulbecco's modified Eagle's medium (DMEM): Ham's F10 medium (1:1) supplemented with 0.01 mM nonessential amino acids, 2 mM L-glutamine, 0.1 mM $\beta$ - mercaptoethnol, soluble recombinant human stem cell factor (SCF; 40ng/ml), human basic fibroblast growth factor (bFGF; 20ng/ml) and human leukemia inhibitory factor (LIF; 20ng/ml). For maintenance of the cells, colonies were passed to fresh feeders every 7-10 days. In all species tested, we were able to obtain and maintain colonies with ES-like morphology. Their developmental potential was tested by alkaline phosphatase (AP) staining and in vitro differentiation assay. For genetic transformation, cells were electroporated with a construct containing the green fluorescent protein (GFP) under the control of the cytomegalovirus (CMV) promoter. GFP-expressing colonies were detected in cattle, rabbits and rats. These results suggest that PGC-derived cells from cattle, goats, rabbits and rats can be isolated, cultured, and genetically transformed, and provide the basis for analyzing their developmental potential and their possible use for the precise genetic modification of these species.

• Journal of agricultural medicine and community health
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• v.32 no.3
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• pp.139-153
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• 2007

Injury in agriculture is a serious public health issue with a major impact on the lives of Korean farmers. It is one of the leading causes of death and is also a major cause of longand short-term disability. In 2001, the social cost of one accident in agricultural machinery was estimated as 97.7-97.8 million won that is 4 fold of farm household income in Korea. Effective prevention and control of injuries requires a system of surveillance that monitors the incidence of injuries, their causes, treatment and outcomes. This requires an integrated system of data collection, analysis and interpretation and communication. Creating effective injury surveillance system in Korea requires to establish a framework for a national agenda. Discussions regarding the development of the framework should address, but not be limited to issues related to Data Holdings and Linkages; Capacity and Skills; Communication; Interconnection; and Surveillance Products. Ideally, an injury surveillance system would meet the information requirements across all sectors, while allowing each to have the ongoing information it needs for its policy and programming needs. This study was carried out to develop a surveillance system of agricultural injuries in Korea. Study subjects were residents who lived in a typical agricultural area (Yangpyung area in Kyung-gi province). The main data sources were reports of village headmen, compared with data of 'National Emergency Management Agency', 'National Health Insurance Corporation', 'Insurance of National Agricultural Cooperative', and 'Emergency Medical Centers'. Each data were reviewed to validate the strengths and weaknesses.

• Journal of the Korean Society of Food Science and Nutrition
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• v.39 no.2
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• pp.172-178
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• 2010

This study was conducted to investigate total polyphenol contents and antioxidative effects of the enzymatic hydrolysates digested by several kinds of carbohydrases from the powder of ripened pumpkin (Cucurbita moschata), sweet pumpkin (C. maxima) and green pumpkin (C. moschata). The total polyphenol contents of all enzymatic hydrolysates from green pumpkin powder were higher than those of ripened and sweet pumpkins. DPPH radical scavenging activities of the enzymatic hydrolysates digested by AMG and Termamyl from green pumpkin were also very strong compared to ripened and sweet pumpkins. However, the most enzymatic hydrolysates of ripened and sweet pumpkin powders, except Viscozyme digestion, were higher superoxide anion scavenging activities than green pumpkin powder. Hydrogen peroxide scavenging activities in the enzymatic digests (not Ultroflo) of green pumpkin were potent compared to other pumpkin powders whereas hydroxyl radical scavenging activities were low at less than 14% in hydrolysates of all pumpkin species. Nitric oxide scavenging activities were very effective in Viscozyme digests of sweet and green pumpkin, and other enzymatic hydrolysates also showed higher activity than $\alpha$-tocopherol control (not BHT).

• Asian-Australasian Journal of Animal Sciences
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• v.17 no.12
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• pp.1729-1735
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• 2004

A total of 140 weaning pigs were used to determine the effects of digestive enzyme supplementation to corn-soybean meal diets on growth performance, physiological changes of small intestine, microorganisms and pH in the gastrointestinal tract. Two kinds of enzyme complex (A, B) were used in this experiment. Pigs were allotted in a completely random design (CRD) to five replicates with four pigs per pen. Diets and water were provided for ad libitum consumption. Treatments included 1) Control: without enzyme supplementation, 2) Enzyme A 0.05%, 3) Enzyme A 0.10%, 4) Enzyme A 0.15%, 5) Enzyme B 0.05%, 6) Enzyme B 0.10%, 7) Enzyme B 0.15% in the diets. A total of 24 crossbred barrows 25.78${\pm}$0.55 kg BW fitted with simple ileal T-cannulas were used to evaluate the effect the enzyme addition on the nutrient digstibility. Pigs were allotted 4 treatments (No enzyme, enzyme A 0.05%, enzyme A 0.1%, enzyme A 0.15%), 6 replicates according to a completely random design (CRD). Another digestibility trial was followed for enzyme complex B. Twenty pigs, average 31.92${\pm}$0.37 kg BW, fitted with simple ileal T-cannulas for digestibility trial. Neither enzyme A nor enzyme B affected on fecal or ileal digestibility of dry matter, gross energy, crude protein, crude fat and crude ash (p>0.05). The apparent fecal digestibilities of all the nutrients were higher in total feces collection method than in indirect method. At the end of feeding trial, 21 pigs were slaughtered for examining the morphological changes of small intestine and the concentration of microorganisms in the ileum and the colon. Growth performance, intestinal morphology and pH of ileum and colon were not affected by the either enzyme complex supplementation (p>0.05). These results suggested that enzyme complex A and enzyme complex B were of no benefit to early-weaned pigs when corn-soybean meal based diet was provided.

• Journal of Distribution Research
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• v.15 no.5
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• pp.61-79
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• 2010

As the interest and the importance of environmental protection increases rapidly, the environment considers to be not only the item that requires a control and a duty fundamental element but the fundamental element to enhance the internection icompetitiveness n so. Along with the collapse of the foreign trade barrier and the quality improvement of a customer's life qu life of a an object is shortened liforder to sectsfy the customer's lemands and as the result of it quaother critical issues rmparding the waste treatment and recasles are now thought to be momentou onlyaddition to the issue of the greenhou e gases reduction that is considered as one of me q issues among many internection ienvironmental issues. To keep up the pace with the environmental flow qube notd countries have nlreal iput their attempts to solve this environmental problems through a number of rmpulactios and acctios and they have showed a great concern for it as it is an internection iproblem that they should aasroaeronlythe aspect of not just a compan isr a country but of the wte e globn ivillage. Sueroenvironmental flow alemaned the logistic stratmpy of a compan iand it brought up the demand for the reverse logistics in a supply chain. For the reason above, the concept of reverse logistics and classification, the comparison with any similar concepts and its necessity are studied in this research through the theoretical consideration and the current state of the reverse logistics is organized in this research by acknowledging the domestic development process and the properties regarding the recycles of resources. In addition, the way to apply to the nation was investigated by comparing with the environmentalism development process, the reverse logistics policies and examples of advanced countries in logistics. The research proposed building of intimate cooperative system between a shipper and logistic service provider for the reverse logistics throughout the whole supply chain, the financial support, amendment of regulations, support Eco-friendly logistic technology development and the propagation, effective logistic system development and others and it is expected to bring a significant meaning to the reverse logistic policy and operation later on.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.35 no.3B
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• pp.408-420
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• 2010

On a Mobile Ad hoc NETwork(MANET), it is difficult to detect and prevent misbehaviors nodes existing between end nodes, as communication between remote nodes is made through multiple hop routes due to lack of a fixed networked structure. Therefore, to maintain MANET's performance and security, a technique to identify misbehaving middle nodes and nodes that are compromise by such nodes is required. However, previously proposed techniques assumed that nodes comprising MANET are in a friendly and cooperative relationship, and suggested only methods to identify misbehaving nodes. When these methods are applied to a larger-scale MANET, large overhead is induced. As such, this paper suggests a system model called Secure Cluster-based MANET(SecCBM) to provide secure communication between components aperANET and to ensure eed. As such, this pand managems suapemisbehavior nodes. SecCBM consists apetwo stages. The first is the preventis pstage, whereemisbehavior nodes are identified when rANET is comprised by using a cluster-based hierarchical control structure through dynamic authentication. The second is the post-preventis pstage, whereemisbehavior nodes created during the course apecommunication amongst nodes comprising the network are dh, thed by using FC and MN tables. Through this, MANET's communication safety and efficiency were improved and the proposed method was confirmed to be suitable for MANET through simulation performance evaluation.

• The Journal of the Korea Contents Association
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• v.18 no.6
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• pp.63-72
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• 2018

This study was conducted to explore the impact of learning strategies on the level of participation in the online collaborative learning process. To do this, We studied 91 students who took professorship courses at A university in the Seoul metropolitan area. At the beginning of the semester, the learning strategies were measured through MSLQ, rehearsal, elaboration, organizing, critical thinking, metacognition, learning management, effort control, peer learning, and seeking help. Next, cooperative tasks were carried out to measure the interaction, and group composition consisted of 4-5 persons. The level of participation was measured by scores given to the messages created for interaction. The process of collaborative learning was divided into the steps of identifying learning goals, learning plans, performing individual learning, sharing learning results, and writing reports. The effects of learning strategies on participation level were analyzed through multiple regression analysis (stepwise selection method). As a result, the learning goal step influenced the highest level of metacognition, and the learning plan is the management of the learning time, the demonstration of the learning execution, the adjustment of the effort, the acquisition of help, the collegial learning, Writing was influenced by organization, elaboration, critical thinking, and critical thinking, metacognition, and elaboration.

• Journal of the Korean Institute of Intelligent Systems
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• v.26 no.4
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• pp.327-333
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• 2016

An autonomous vehicle is a convergence of artificial intelligence and a vehicle which can drive itself while analyzing the real-time situation on a road without a driver. A lot of research achievements have been revealed through the media and Google is considered to be the best leading company in this field. The use of patent information which contains various information such as bibliographic data and information about technologies is a good way to find out the R&D direction of a company and develop a reasonable strategy. This study is aimed at investigating the direction to which Google focuses its R&D capabilities and establishing strategies for technology development. Google's patents about autonomous vehicles were collected and the degree of research bias was analyzed using Social Network Analysis based on citations indicating the quality of a patent. Based on the results, the strategies for technology development was eventually proposed. As a result, it was revealed that Google focused its R&D capabilities on the part of hardware control to make up for its lack of hardware-oriented technologies. As of now, Google obtained remarkable achievements, so it seems reasonable that last-movers consider cooperative research with Google.

• Journal of Korean Society of Forest Science
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• v.71 no.1
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• pp.1-8
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• 1985

In the study, the log pricing process by region and marketing agencies was surveyed in order to analyze the domestic timber marketing situation, and to propose improvements. The results obtained show that the market channel configuration for domestic timber is simple compared with that for the agricultural products. The log felling contractor is the lead agency in the stumpage market because of the lack of market information of most forest owners. However, the log assembler, who has ample funds, seized leadership in the market channel because most felling contractors, deal only with small timber quantities, and are usually short of funds. Also the variety of log scaling methods is a serious factor confusing domestic timber marketing. Therefore, the following steps are proposed; 1) the provision of market information to forest owners, 2) the institutional control of felling contractors, 3) the establishment of cooperative log collection centers, 4) the improvement of log quality.

• Journal of the Computational Structural Engineering Institute of Korea
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• v.21 no.3
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• pp.247-252
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• 2008

Recently, the virtual construction system in which project participants efficiently share and control the information throughout the life-cycle of construction project using 3D object models is being developed all over the world. In this paper, a design of interface between 3D object model of structures and structural analysis system that is essential for the analysis and design of civil structures in the virtual space is treated. The relation parametric modeling technique that is needed to make the 3D object models and the construction method of product breakdown structure(PBS) that considers the several parameters for the structural analysis are presented. PBS is built so that it is possible to extract needed attribute information from 3D object model and to apply it to the structural analysis. Design methodology for interface program is proposed that several numerical values determined by the cooperative work same as structural analysis are delivered to 3D object models without additional work. An interface program between 3D object models and structural analysis system developed based on the proposed method would be effectively used to develop virtual construction system.