• 대한화장품학회지
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• 제50권2호
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• pp.119-129
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• 2024

인체 피부 림프계는 간질액을 배출하고 면역 시스템을 활성화하는 중요한 역할을 한다. 자외선과 자연적인 노화와 같은 환경 요인들은 종종 이러한 림프관의 구조적 변화를 일으키며, 이로 인해 피부 기능 장애를 발생시키기도 한다. 그러나 이러한 연구를 위한 동물 실험 대체 방안이 없기 때문에 여전히 연구를 진행하기엔 적합하지 않은 제한 사항들이 존재한다. 인체 피부 림프계를 더 잘 이해하고 림프관 형성에 관련된 분자 및 생리학적 변화를 조사하기 위해, 생체 모방 미세유체 플랫폼인 'skin-lymph-on-a-chip'을 제작하여, 새로운 체외 인체 피부 림프 모델을 개발하였다. 간단히 말해, 이 플랫폼은 체외에서 분화된 일차 정상 인간 표피 각질형성세포(NHEKs)와 피부 림프 내피세포(HDLECs)를 공동 배양하는 것을 의미한다. 약 500 시간 동안 자연 발효를 통해 확보하고 집약된 인삼 뿌리 추출물인 Lymphanax^TM의 림프관 형성 효과를 평가하기 위해 해당 시스템에 적용하였고, 분자 수준 요인들의 변화는 딥러닝 기반 알고리즘을 사용하여 분석하였다. 결론적으로, Lymphanax^TM는 skin-lymph-on-a-chip에서 건강한 림프관 형성을 촉진하였고, 그 성분들이 칩내에서 분화된 NHEKs를 거의 침투하지 않는 결과를 통해, HDELCs에 간접적으로 영향을 미쳤음을 확인하였다. 전반적으로, 이 연구는 기존과 차별화된 체외 인체 피부 림프모델 시스템의 확보와 더불어 이를 통한 Lymphanax^TM의 림프 활성화 효과에 대한 새로운 관점을 제공한다.

• 한국식품영양과학회지
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• 제45권1호
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• pp.20-26
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• 2016

본 연구는 민들레잎을 노루궁뎅이버섯 균사체로 발효한 후물과 에탄올로 추출하여 추출용매별 및 발효 전과 발효 후의 항산화 및 항염증 활성을 비교 평가하였다. 추출물의 항산화 활성을 탐색하고자 총 폴리페놀 함량, 총 플라보노이드 함량, DPPH 라디칼 소거능, ABTS를 이용한 라디칼 소거 활성, FRAP을 이용한 총 항산화능을 측정하였다. 또한 RAW 264.7 대식세포를 이용하여 NO 생성량, 염증관련 단백질 및 사이토카인의 변화를 측정하였다. 민들레잎을 발효한 후 항산화 활성은 감소하였으나 오히려 항염증 활성은 증가하는 경향을 나타내었다. 특히 발효 민들레잎의 에탄올 추출물은 NO 생성 및 iNOS 단백질 발현을 효과적으로 억제하였으며 염증성 사이토카인인 IL-6를 억제하는 효과가 우수하였다. 이상의 결과로 항염증 소재로서 노루궁뎅이버섯 균사체를 이용한 발효 민들레잎의 에탄올 추출물의 활용 가능성을 확인하였다.

• Korean Chemical Engineering Research
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• 제49권1호
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• pp.21-27
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• 2011

메탄올 수증기 개질반응에 대한 적용가능성을 파악하기 위하여 메탄올 합성용 촉매인 ICI-M45와 수성가스 전환반응용 촉매인 MDC-3와 MDC-7을 비교 연구하였다. 또한 수성가스전환 반응에 대한 세 촉매의 비교실험도 수행하였다. 그 결과 MDC-7이 메탄올 수증기 개질반응에서 가장 높은 전화율을 보였으며, $H_2$와 $CO_2$ 생성속도 또한 높게 나타났다. 수성가스 전환반응용 촉매인 MDC-7과 메탄올 합성촉매인 ICI-M45를 이용하여 촉매 충진 방법에 따른 메탄올의 전화율에서의 변화를 살펴본 결과, MDC-7 단독보다 낮은 메탄올의 전화율을 보였다. 수성가스 전환반응에서도 DC- 7, MDC-3, 그리고 ICI-M45의 순으로 반응성이 감소하였다. 상기 두 반응에서 MDC-7이 가장 우수한 이유로는 높은 비표면적과 Cu의 분산도, 그리고 적절한 Cu와 Zn의 비율에 기인함을 확인할 수 있었다.

• Journal of Microbiology and Biotechnology
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• 제25권2호
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• pp.227-233
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• 2015

Two recombinant arabinosyl hydrolases, α-_L-arabinofuranosidase from Geobacillus sp. KCTC 3012 (GAFase) and endo-(1,5)-α-_L-arabinanase from Bacillus licheniformis DSM13 (BlABNase), were overexpressed in Escherichia coli, and their synergistic modes of action against sugar beet (branched) arabinan were investigated. Whereas GAFase hydrolyzed 35.9% of _L-arabinose residues from sugar beet (branched) arabinan, endo-action of BlABNase released only 0.5% of _L-arabinose owing to its extremely low accessibility towards branched arabinan. Interestingly, the simultaneous treatment of GAFase and BlABNase could liberate approximately 91.2% of _L-arabinose from arabinan, which was significantly higher than any single exo-enzyme treatment (35.9%) or even stepwise exo- after endo-enzyme treatment (75.5%). Based on their unique modes of action, both exo- and endo-arabinosyl hydrolases can work in concert to catalyze the hydrolysis of arabinan to _L-arabinose. At the early stage in arabinan degradation, exo-acting GAFase could remove the terminal arabinose branches to generate debranched arabinan, which could be successively hydrolyzed into arabinooligosaccharides via the endo-action of BlABNase. At the final stage, the simultaneous actions of exo- and endo-hydrolases could synergistically accelerate the _L-arabinose production with high conversion yield.

• 대한방사선기술학회지:방사선기술과학
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• 제25권2호
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• pp.47-55
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• 2002

유방촬영에 대한 화질과 평균유선조직선량에 대하여 실험한 결과 반가층은 장치마다 큰 차이를 나타내지 않았으며, 자동노출기구를 사용할 경우 정확성에 문제가 있으므로 확인이 필요하다. 유방촬영에 사용되는 자동현상기 또한 꼭 관리가 필요하며, 유방촬영 시 사용되는 증감지/필름에 따라서 팬톰 촬영 식별능에 많은 영향을 받고 있으므로 선택 시 신중을 기해야 한다. 유방두께 4.2 cm에 대한 평균유선조직 선량은 관전압에 따라 달라지며, ACR기준인 3.0 mGy보다 훨씬 적은 $0.26{\sim}1.39\;mGy$로 나타났다. 이상에서 알 수 있듯이 유방촬영에서는 촬영장치의 성능뿐만 아니라 HVL, AEC, 자동현상기 관리, 증감지/필름 등 모두 관리가 되어야 하며, 이로 인해 평균유선조직선량은 더 줄일 수 있을 것이다.

• Journal of Animal Science and Technology
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• 제63권4호
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• pp.934-953
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• 2021

Ciglitazone is a member of the thiazolidinedione family, and specifically binds to peroxisome proliferator-activated receptor-γ (PPARγ), thereby promoting adipocyte differentiation. We hypothesized that ciglitazone as a PPARγ ligand in the absence of an adipocyte differentiation cocktail would increase adiponectin and adipogenic gene expression in bovine satellite cells (BSC). Muscle-derived BSCs were isolated from six, 18-month-old Yanbian Yellow Cattle. The BSC were cultured for 96 h in differentiation medium containing 5 µM ciglitazone (CL), 10 µM ciglitazone (CM), or 20 µM ciglitazone (CH). Control (CON) BSC were cultured only in a differentiation medium (containing 2% horse serum). The presence of myogenin, desmin, and paired box 7 (Pax7) proteins was confirmed in the BSC by immunofluorescence staining. The CL, CM, and CH treatments produced higher concentrations of triacylglycerol and lipid droplet accumulation in myotubes than those of the CON treatment. Ciglitazone treatments significantly increased the relative expression of PPARγ, CCAAT/enhancer-binding protein alpha (C/EBPα), C/EBPβ, fatty acid synthase, stearoyl-CoA desaturase, and perilipin 2. Ciglitazone treatments increased gene expression of Pax3 and Pax7 and decreased expression of myogenic differentiation-1, myogenin, myogenic regulatory factor-5, and myogenin-4 (p < 0.01). Adiponectin concentration caused by ciglitazone treatments was significantly greater than CON (p < 0.01). RNA sequencing showed that 281 differentially expressed genes (DEGs) were found in the treatments of ciglitazone. DEGs gene ontology (GO) analysis showed that the top 10 GO enrichment significantly changed the biological processes such as protein trimerization, negative regulation of cell proliferation, adipocytes differentiation, and cellular response to external stimulus. Kyoto Encyclopedia of Genes and Genomes pathway analysis showed that DEGs were involved in the p53 signaling pathway, PPAR signaling pathway, biosynthesis of amino acids, tumor necrosis factor signaling pathway, non-alcoholic fatty liver disease, PI3K-Akt signaling pathway, and Wnt signaling pathway. These results indicate that ciglitazone acts as PPARγ agonist, effectively increases the adiponectin concentration and adipogenic gene expression, and stimulates the conversion of BSC to adipocyte-like cells in the absence of adipocyte differentiation cocktail.

• Journal of Ginseng Research
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• 제42권1호
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• pp.116-121
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• 2018

Background: Black ginseng (BG) has greatly enhanced pharmacological activities relative to white or red ginseng. However, the effect and molecular mechanism of BG on muscle growth has not yet been examined. In this study, we investigated whether BG could regulate myoblast differentiation and myotube hypertrophy. Methods: BG-treated C2C12 myoblasts were differentiated, followed by immunoblotting for myogenic regulators, immunostaining for a muscle marker, myosin heavy chain or immunoprecipitation analysis for myogenic transcription factors. Results: BG treatment of C2C12 cells resulted in the activation of Akt, thereby enhancing hetero-dimerization of MyoD and E proteins, which in turn promoted muscle-specific gene expression and myoblast differentiation. BG-treated myoblasts formed larger multinucleated myotubes with increased diameter and thickness, accompanied by enhanced Akt/mTOR/p70S6K activation. Furthermore, the BG treatment of human rhabdomyosarcoma cells restored myogenic differentiation. Conclusion: BG enhances myoblast differentiation and myotube hypertrophy by activating Akt/mTOR/p70S6k axis. Thus, our study demonstrates that BG has promising potential to treat or prevent muscle loss related to aging or other pathological conditions, such as diabetes.

• 유통과학연구
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• 제13권10호
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• pp.123-133
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• 2015

Purpose - The Korean government has devised intermodal transportation policies and granted subsidies to shippers and logistics companies that made a conversion of transportation means through the policies. This provides support by expanding the complex uniform railroad transportation and overhauling the deteriorated railroad facilities. As for 2013, however, the freight transportation percentage of railroad was 4.5% in tons and 8.5% in ton kilometers. Meanwhile, since the 1990s, developed countries such as the U.S. and Europe have been trying to expand intermodal freight transport with a legal and institutional support to build a logistics system corresponding with social and economic environmental changes. In this study, I set out to examine the effects of the intermodal freight transport policies in the EU and the U.S., and to explore the direction of setting up a rail intermodal transport system in South Korea. Research design, data, and methodology - The paper used a qualitative research methodology through the literature review. First, was an overview of Intermodal transportation in the EU, U.S. and UN. Second, it describes the development of transport in Europe and the U.S. with particular emphasis on intermodal freight transport. Third, it explores the direction of setting up a intermodal freight transport in South Korea. The last section contains concluding remarks. Results - As for the EU, it has been promoting integration between transport and intermodal logistics network designs while utilizing ITS or ICT and supports for rail freight intermodal by giving reduction to a facilities fee or subsidizing for rail freight in order to minimize the cost of external due to freight transport. On the other hand, as for the U.S., it has been made up of an industrial-led operating project and has been promoting it to improve accessibility between intermodal hubs and cargo terminals through intermodal corridor program, and an intermodal cargo hub access corridor projects, etc. Moreover, it has tried to construct intermodal transport system using ITS or ICT and to remove Barrier. As a result, in these countries, the proportion of intermodal freight transport is going to be the second significant transport compared with rail and maritime transport. An Effective rail intermodal transport system is needed in South Korea, as seen in the case of these countries. In order to achieve this object, the following points are required to establish radical infrastructure policy; diversify investment financing measures taken under public-private partnerships, legal responsibilities, improvement of utilization of existing facilities to connect the railway terminal and truck terminal, and enhancement service competitiveness through providing cargo tracking and security information that combines the ITS and ICT. Conclusions - This study will be used as a basis for policy and support for intermodal freight transport in South Korea. In the future, it is also necessary to examine from the perspective of the shipper companies using the rail intermodal transport, ie, recognition of shipper, needed institutional supports, and transportation demand forecasting and cost-effective analysis of the railway infrastructure systems improvement.

• Asian-Australasian Journal of Animal Sciences
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• 제8권6호
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• pp.587-594
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• 1995

In order to estimate the nutritive value of roasted full-fat soybean (FFS) in carp diet, growth performance and excretion of protein and phosphorus were examined using carps having mean body weight of 111 g. Growth trial was conducted for 4 weeks using the fishes fed 5 diets (Control, $F_{24}S_{13}$, $F_{16}S_{27}$, $F_8S_{40}$ and $F_0S_{56}$) containing 32%, 24%, 16%, 8% and 0% of fish meal (F) and 0%, 13%, 27%, 40% and 56% of full-fat soybean, respectively. A total of 800 fishes were allotted randomly by groups of 40 to 5 treatments with 4 replicates per treatment. Fishes were fed to station eight times daily. Feeding trial was conducted for 4 weeks. As dietary FFS increased from 0% to 56%, weight gain of fish decreased from 91 g to 39 g and feed conversion ratio increased from 1.06 to 1.95. Protein efficiency ratio (PER) was highest (2.35) in control group which had the highest protein intake, while PER significantly decreased with decrease in protein intake as dietary FFS level increased. Although dietary protein and energy levels were maintained constant, protein excretion per kg weight gain varied from 273 g to 579 g for the groups control and $F_0S_{56}$, respectively. However, the value for control group was not significantly different to those for the groups $F_{24}S_{13}$ and $F_{16}S_{27}$. On the other hand, the highest protein retention efficiency was found in group fed the diet $F_{24}S_{13}$. Fish fed the diet $F_8S_{40}$ excreted the lowest phosphorus (P) based on kg weight gain showing the highest P retention efficiency of 62%. P excretion per kg feed intake was in the range of 5 g to 10 g for the groups $F_8S_{40}$ and $F_{24}S_{13}$, respectively. The present results indicated that as dietary FFS level increased, growth performance and feed utilization decreased while excretion of protein and phosphorus increased. Therefore, it was concluded that more than 25% substitution by FFS for fish meal could exert negative effects on growth and feed utilization of carp.

• 생명과학회지
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• 제20권6호
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• pp.811-818
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• 2010

Sphingomnas paucibilis NK2000 균주를 사용한 젤란의 최적 교반속도 및 통기량은 각각 400 rpm 및 1.0 vvm이었다. 이 균주를 사용하여 젤란의 생산성을 향상시키기 위한 포도당의 최적 첨가시기는 배양을 시작한 36시간이었다. 젤란의 생산성을 향상시키기 위한 5가지 방법, 1) 포도당을 첨가하지 않는 방법, 2) 배양 36시간 후에 포도당을 첨가하지만 교반속도 및 통기량을 변화시키지 않는 방법, 3) 배양 36 시간 후에 포도당을 첨가하고 교반속도를 400 rpm에서 600 rpm으로 변화시키는 방법, 4) 배양 36 시간 후에 포도당을 첨가하고 교반속도를 400 rpm에서 600 rpm으로 증가시키고 통기량을 1.0 vvm에서 1.5 vvm으로 증가시키는 방법 및 5) 배양 36 시간 후에 포도당을 첨가하고 교반속도를 400 rpm에서 600 rpm으로 증가시키고 통기량을 1.0 vvm에서 2.0 vvm으로 증가시키는 방법 등을 실험한 결과, 젤란의 생산성은 각각 5.19, 5.74, 6.73, 7.93, 및 9.40 g/l이었으며, 변환률은 각각 26.0, 19.1, 22.4, 26.4, and 31.3%이었다. 최적의 방법으로 생산한 젤란의 생산농도 및 포도당 전환율은 포도당을 첨가하지 않은 방법으로 생산한 젤란의 생산농도 및 포도당 전환율에 비하여 각각 1.81 및 1.20 배 증가하였다.