• Title/Summary/Keyword: Conventional Expression

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Comparison of Invariant NKT Cells with Conventional T Cells by Using Gene Set Enrichment Analysis (GSEA)

  • Oh, Sae-Jin;Ahn, Ji-Ye;Chung, Doo-Hyun
    • IMMUNE NETWORK
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    • v.11 no.6
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    • pp.406-411
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    • 2011
  • Background: Invariant Natural killer T (iNKT) cells, a distinct subset of CD1d-restricted T cells with invariant $V{\alpha}{\beta}$ TCR, functionally bridge innate and adaptive immunity. While iNKT cells share features with conventional T cells in some functional aspects, they simultaneously produce large amount of Th1 and Th2 cytokines upon T-cell receptor (TCR) ligation. However, gene expression pattern in two types of cells has not been well characterized. Methods: we performed comparative microarray analyses of gene expression in murine iNKT cells and conventional $CD4^+CD25^-$ ${\gamma}{\delta}TCR^-$ T cells by using Gene Set Enrichment Analysis (GSEA) method. Results: Here, we describe profound differences in gene expression pattern between iNKT cells and conventional $CD4^+CD25^-$ ${\gamma}{\delta}TCR^-$ T cells. Conclusion: Our results provide new insights into the functional competence of iNKT cells and a better understanding of their various roles during immune responses.

Identification of the Housekeeping Genes Using Cross Experiments via in silico Analysis

  • Yim, Won-Cheol;Keum, Chang-Won;Kim, Sae-Hwan;Jang, Cheol-Seong;Lee, Byung-Moo
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.55 no.4
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    • pp.371-378
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    • 2010
  • For sensitive and accurate gene expression analysis, normalization of gene expression data against housekeeping genes is required. There are conventional housekeeping gene (e.g. ACT) that primarily function as an internal control of transcription. In this study, we performed an in silico analysis of 278 rice gene expression samples (GSM) in order to identify the gene that is most consistently expressed. Based on this analysis, we identified novel candidate housekeeping genes that displayed improved stability among the cross experimental conditions. Furthermore four of the most conventional housekeeping genes were included in our 30 other housekeeping genes among the most stable genes. Therefore, these 30 genes can he used to normalize transcription results in gene expression studies on rice at a broad range of experimental conditions.

A Face Expression Recognition Method using Histograms (히스토그램을 이용한 얼굴 표정 인식 방법)

  • Huh, Kyung Moo
    • Journal of Institute of Control, Robotics and Systems
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    • v.20 no.5
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    • pp.520-525
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    • 2014
  • Generally, feature area detection methods are widely used for face expression recognition by detecting the feature areas of human eyes, eyebrows and mouth. In this paper, we proposed a face expression recognition method using the histograms of the face, eyes and mouth for many applications including robot technology. The experimental results show that the proposed method has a new type of face expression recognition capability compared to conventional methods.

Construction of nervous necrosis virus (NNV) genome-based DNA replicon vectors for the delivery of foreign antigens

  • Jeong In Yang;Ki Hong Kim
    • Journal of fish pathology
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    • v.37 no.1
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    • pp.1-8
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    • 2024
  • The advantages of replicon vectors of RNA viruses include a high ability to stimulate innate immunity and exponential amplification of target mRNA leading to high expression of foreign antigens. The present study aimed to construct a DNA-layered nervous necrosis virus (NNV) replicon vector system in which the capsid protein gene was replaced with a foreign antigen gene and to compare the efficiency of foreign antigen expression between the conventional DNA vaccine vector and the present replicon vector. We presented the first report of a nodavirus DNA replicon-based foreign antigen expression system. Instead of a two-vector system, we devised a one-vector system containing both an NNV RNA-dependent RNA polymerase cassette and a foreign antigen-expressing cassette. This single-vector approach circumvents the issue of low foreign protein expression associated with the low co-transfection efficiency of a two-vector system. Cells transfected with a vector harboring hammerhead ribozyme-fused RNA1 and RNA2 (with the capsid gene ORF replaced with VHSV glycoprotein ORF) exhibited significantly higher transcription of the VHSV glycoprotein gene compared to cells transfected with either a vector without hammerhead ribozyme or a conventional DNA vaccine vector expressing the VHSV glycoprotein. Furthermore, the transcription level of the VHSV glycoprotein in cells transfected with a vector harboring hammerhead ribozyme-fused RNA1 and RNA2 showed a significant increase over time. These results suggest that NNV genome-based DNA replicon vectors have the potential to induce stronger and longer expression of target antigens compared to conventional DNA vaccine vectors.

A Study on Conventional Expression of Hangul Ganchal and Email (조선시대 한글 간찰과 이메일의 상투적 표현 고찰)

  • Jeon, Byeong-yong
    • (The)Study of the Eastern Classic
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    • no.49
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    • pp.431-459
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    • 2012
  • The purpose of this article is to compare and analyze the conventional expression of Hangul Ganchal in Cheosun Dynasty and Email. Conventional expression is used remarkably in introductions and conclusions. In introduction, it is used for addressing and safety greetings while in conclusion, it is used for closing address and closing words. In Cheosun Dynasty, an envelope of Ganchal only included the details of the receiver because the letter was genuinely delivered by someone who knew the receiver and the sender very well. An envelope of Ganchal is applicable to the screen of the internet which is used for emailing. In an email, we see the name of the sender and the title of the text and once we click the title, we are able to view the text. The difference between the Ganchal and the email was reflected on how the receiver's detail showed on Ganchal and the email show the sender's details. In a case of addressing in a letter while using the conventional expression, we can see how we use "To~" in humble term and " ~께" in a honorific term. We confirmed that the conventional expression has not yet settled in both of the Gnachal and email for the seasonal greetings. The safety greetings comprised with both of the senders' and receivers' latest updates. In Ganchal, this composition is well described conventionally, whereas in emails, only the receivers' latest news are written but the senders' latest updates are hard to be seen throughout the text. In Ganchal's closing section, the closing address and closing words were expressed conventionally. However, in the case of email; those were again hard to be found throughout. To conclude, in Ganchal the conventional expression was developed and placed in 16thcentury(Sun-eon) when there was a focus in our native language. In 17thcentury(Hyeon-eon), it stood still for a sometime and moved on to 19thcentury(Jing-eon) when there was a strong in fluence of Hangul Ganchal, which resulted in regression to the conservative expression. In general, we are able to confirm that the conventional expression is slowly disappearing.

A Study on the Deconstruction of Perspective in Fashion Illustrations (패션 일러스트레이션에 나타난 원근법 해체에 대한 연구)

  • Kim, Soon-Ja
    • Journal of the Korea Fashion and Costume Design Association
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    • v.17 no.2
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    • pp.93-109
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    • 2015
  • Advent of diverse media and advancement in digital technology significantly affected our perception of time and space. By utilizing digital media actively, fashion illustration has come to go beyond the limitation on conventional perspective expression. This study aims to examine the phenomenon of deconstruction of perspective in fashion illustration, and to analyze a variety of space expressions and effects of visual expression in fashion illustration. By way of literature research for the study, theoretical data are reviewed about basic concepts of conventional perspective and the characteristics and aesthetic concept of perspective deconstruction expression in visual art. By way of an empirical study, fashion illustrations that represent the deconstruction of perspective are classified and analyzed characteristics and image effects of such expressions. Deconstruction of perspective in fashion illustration find in expression of spaces by means of multi-space, multi-layer space, reverse perspective, perspective through the exaggeration, multi-perspective space, and panoramic visual condition. Fashion illustration expresses visual confusion or fantasy rather than creating realistic spaces that have utilized reasonable perspective. Fashion illustration is moving toward a trait with disharmony and an in-depth visionary space by overlapping or mixing spaces differing from each other, and by means of such peculiar and unrealistic expressions, unfamiliar images are created.

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Effects of Immune Stress on Performance Parameters, Intestinal Enzyme Activity and mRNA Expression of Intestinal Transporters in Broiler Chickens

  • Feng, Y.;Yang, X.J.;Wang, Y.B.;Li, W.L.;Liu, Y.;Yin, R.Q.;Yao, J.H.
    • Asian-Australasian Journal of Animal Sciences
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    • v.25 no.5
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    • pp.701-707
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    • 2012
  • Immune stress is the loss of immune homeostasis caused by external forces. The purpose of this experiment was to investigate the effects of immune stress on the growth performance, small intestinal enzymes and peristalsis rate, and mRNA expression of nutrient transporters in broiler chickens. Four hundred and thirty-two 1-d-old broilers (Cobb500) were randomly assigned to four groups for treatment; each group included nine cages with 12 birds per cage. Group 1 = no vaccine (NV); Group 2 = conventional vaccine (CV); group 3 = lipopolysaccharide (LPS)+conventional vaccine (LPS); group 4 = cyclophosphamide (CYP)+conventional vaccine (CYP). The results demonstrated that immune stress by LPS and CYP reduced body weight gain (BWG), feed intake (FI), small intestine peristalsis rate and sIgA content in small intestinal digesta (p<0.05). However, the feed conversion ratio (FCR) remained unchanged during the feeding period. LPS and CYP increased intestinal enzyme activity, relative expression of SGLT-1, CaBP-D28k and L-FABP mRNAs (p<0.05). LPS and CYP injection had a negative effect on the growth performance of healthy broiler chickens. The present study demonstrated that NV and CV could improve growth performance while enzyme activity in small intestine and relative expression of nutrient transporter mRNA of NV and CV were decreased in the conditions of a controlled rational feeding environment. It is generally recommended that broilers only need to be vaccinated for the diseases to which they might be exposed.

A Study on Conventional Expression of Hangul Ganchal (한글 간찰(簡札)의 상투적(常套的) 표현(表現) 고찰(考察))

  • Jeon, Byeong-yong
    • (The)Study of the Eastern Classic
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    • no.37
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    • pp.279-306
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    • 2009
  • This study is purposed to observe conventional expression of Hangul Ganchal. The conventional expression of Hangeul Ganchal had set a pattern from 16th century to 17the century after establishing its regular form. It is mainly found in the introduction and the conclusion of the letters. Especially, they are concretely shown at an address form and greeting in the former, a farewell and the close in the latter. Generally, a native tongue was often used in address form at Suncheon Kim's Eongan in 16th century and Hyun-Poong Kwak's Eongan in 17th century. However, a Chinese character was commonly found in the introduction at Jingbo-Eongan-Dok in 19th century. Specifically, in case of response, the conventional expression of 'read your letter' was added in greeting part. In Suneon of 16th century, this type was gradually established to the form, and in Hyuneon of 17th century, a native tongue set a pattern. However, Jingeon of 19th century, a Chinese character was mainly used instead of a native tongue. The changing form of farewell and the close showed similar pattern as the aspect of greeting form. They seemed to be more simple and repetitive in Hyuneon compared with the form of Suneon. Furthermore, a Chinese character was dominated in the conclusion of letters in Jingeon. Consequently, the paper examined the two types of letter between Hangul letters and Chinese character letters were strongly associated with each other. It is expected that more details research will be coped with next study.

Facial Data Visualization for Improved Deep Learning Based Emotion Recognition

  • Lee, Seung Ho
    • Journal of Information Science Theory and Practice
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    • v.7 no.2
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    • pp.32-39
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    • 2019
  • A convolutional neural network (CNN) has been widely used in facial expression recognition (FER) because it can automatically learn discriminative appearance features from an expression image. To make full use of its discriminating capability, this paper suggests a simple but effective method for CNN based FER. Specifically, instead of an original expression image that contains facial appearance only, the expression image with facial geometry visualization is used as input to CNN. In this way, geometric and appearance features could be simultaneously learned, making CNN more discriminative for FER. A simple CNN extension is also presented in this paper, aiming to utilize geometric expression change derived from an expression image sequence. Experimental results on two public datasets (CK+ and MMI) show that CNN using facial geometry visualization clearly outperforms the conventional CNN using facial appearance only.

Low-power/high-speed DCT structure using common sub-expression sharing (Common sub-expression sharing을 이용한 고속/저전력 DCT 구조)

  • Jang, Young-Beom;Yang, Se-Jung
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.29 no.1C
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    • pp.119-128
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    • 2004
  • In this paper, a low-power 8-point DCT structure is proposed using add and shift operations. Proposed structure adopts 4 cycles for complete 8-point DCT in order to minimize size of hardware and to enable high-speed processing. In the structure, hardware for the first cycle can be shared in the next 3 cycles since all columns in the DCT coefficient matrix are common except sign. Conventional DCT structures implemented with only add and shift operation use CSD(Canonic Signed Digit) form coefficients to reduce the number of adders. To reduce the number of adders further, we propose a new structure using common sub-expression sharing techniques. With this techniques, the proposed 8-point DCT structure achieves 19.5% adder reduction comparison to the conventional structure using only CSD coefficient form.