• IMMUNE NETWORK
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• 제21권1호
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• pp.9.1-9.8
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• 2021

The most important characteristics of coronavirus disease 2019 (COVID-19) transmission that makes it difficult to control are 1) asymptomatic and presymptomatic transmission, 2) low incidence or lack of dominant systemic symptoms such as fever, 3) airborne transmission that may need a high infectious dose, and 4) super-spread events (SSEs). Patients with COVID-19 have high viral loads at symptom onset or even a few days prior to symptom onset, and most patients with COVID-19 have only mild respiratory symptoms or merely pauci-/null-symptoms. These characteristics of the virus enable it to easily spread to the community because most patients are unaware of their potential infectivity, and symptom-based control measures cannot prevent this type of transmission. Furthermore, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is also capable of airborne transmission in conditions such as aerosol-generating procedures, under-ventilated indoor spaces, and over-crowded areas. In this context, universal mask-wearing is important to prevent both outward and inward transmission until an adequate degree of herd immunity is achieved through vaccination. Lastly, the SSEs of SARS-CoV-2 transmission emphasize the importance of reducing contacts by limiting social gatherings. The above-mentioned transmission characteristics of SARS-CoV-2 have culminated in the failure of long-lasting quarantine measures, and indicate that only highly effective vaccines can keep the communities safe from this deadly, multifaceted virus.

• Asian Pacific Journal of Cancer Prevention
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• 제16권12호
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• pp.4927-4935
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• 2015

Northeastern India is a major nasopharyngeal carcinoma (NPC) high risk-area although the rest of the country has very low incidence. A case-control study of 105 NPC cases and 115 controls was conducted to identify the potential risk factors for NPC development in this region. Information was collected by interviewer about socio-demographic characteristics, cigarette smoking, alcohol consumption, dietary history, occupational history, and a family history of cancer. Epstein-Barr viral load was assayed from the blood DNA by real time PCR. Associations between GSTs genotypes, cytochrome P450 family including CYP1A1, CYP2E1 and CYP2A6 polymorphisms and susceptibility to relationship between the diseases were studied using PCR-RFLP assay. Results indicate that Epstein-Barr virus load was significantly higher in patients compared to controls (p<0.0001). Furthermore, concentration of blood EBV-DNA was significantly higher in advanced stage disease (Stage III and IV) than in early stage disease (Stage I and II) (p<0.05). Presence of CYP2A6 variants that reduced the enzyme activity was significantly less frequent in cases than controls. Smoked meat consumption, exposure to smoke, living in poorly ventilated house and alcohol consumption were associated with NPC development among the population of Northeastern India. Thus, overall our study revealed that EBV viral load and genetic polymorphism of CYP2A6 along with living practices which include smoked meat consumption, exposure to smoke, living in poorly ventilated houses and alcohol consumption are the potential risk factors of NPC in north eastern region of India. Understanding of the risk factors and their role in the etiology of NPC are helpful forpreventive measures and screening.

• 한국동물위생학회지
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• 제13권1호
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• pp.96-102
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• 1990

The immune responses of commercial layer chickens against Newcastle disease(ND) were compared among different administration methods and times of vaccination during 4 weeks of age. A total of 372 day-old chickens were divided into 4 groups of 93 birds each. Each of 3 groups was received a commercially available B$_1$live vaccine via drinking water, eye instillation or spray method at one, 14 and 28 days of age. One group was used as an unvaccinated control. At two and 4 weeks after each time of vaccination, 15 birds from each group were collected randomly out and challenged with virulent ND virus at the dose of $10^5E1D_{50}$ per bird. Ten to 15 birds from each group were bled at two weeks intervals from day old to 8 weeks of age for hemagglutination inhibition antibody titer, The protection rate was generally low regardless of the times of vaccination although two or more times vaccination gave higher protection than once vaccination. The low protection was considered due to low titer of the vaccine used since the vaccine titer was less than $10^{3.5}EID_{50}$ per bird. Spray method gave better protection compared to eye instillation or drinking water method which resulted in lowest response. Majority of birds showed clinical signs of ND between 3 and 6 days after challenge. Death occured one or two days after onset of symptoms. Major clinical signs observed were depression(94%), anorexia(84%), diarrhoea(29%), difficult breath(15%) and torticollis(10%). Hemorrhagic lesions on post mortem were seen in duodenum(51%), trachea(35%), illeum(13%), ceacal tonsil(11%), proventriculus(10%) and some other odrgans.

• Asian Pacific Journal of Cancer Prevention
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• 제17권8호
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• pp.3663-3673
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• 2016

Cervical cancer (CaCx) is the second most fatal cancer contributing to 14% of cancers in Indian females, which account for 25.4% and 26.5% of the global burden of CaCx prevalence and mortality, respectively. Persistent infection with high-risk human papilloma virus (HPV- strains 16 and 18) is the most important risk factor for precursors of invasive CaCx. Comprehensive prevention strategies for CaCx should include screening and HPV vaccination. Three screening modalities for CaCx are cytology, visual inspection with acetic acid, and HPV testing. There is no Indian national policy on CaCx prevention, and screening of asymptomatic females against CaCx is practically non-existent. HPV vaccines can make a major breakthrough in the control of CaCx in India which has high disease load and no organized screening program. Despite the Indian Government's effort to introduce HPV vaccination in the National Immunization Program and bring down vaccine cost, challenges to implementing vaccination in India are strong such as: inadequate epidemiological evidence for disease prioritization, duration of vaccine use, parental attitudes, and vaccine acceptance. This paper reviews the current epidemiology of CaCx and HPV in India, and the current status of HPV vaccination in the country. This article stresses the need for more research in the Indian context, to evaluate interventions for CaCx and assess their applicability, success, scalability and sustainability within the constraints of the Indian health care system.

• Asian-Australasian Journal of Animal Sciences
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• 제31권4호
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• pp.489-498
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• 2018

Objective: Foot and mouth disease (FMD) and porcine reproductive and respiratory syndrome (PRRS) are major diseases that interrupt porcine production. Because they are viral diseases, vaccinations are of only limited effectiveness in preventing outbreaks. To establish an alternative multi-resistant strategy against FMD virus (FMDV) and PRRS virus (PRRSV), the present study introduced two genetic modification techniques to porcine cells. Methods: First, cluster of differentiation 163 (CD163), the PRRSV viral receptor, was edited with the clustered regularly interspaced short palindromic repeats-CRISPR-associated protein 9 technique. The CD163 gene sequences of edited cells and control cells differed. Second, short hairpin RNA (shRNAs) were integrated into the cells. The shRNAs, targeting the 3D gene of FMDV and the open reading frame 7 (ORF7) gene of PRRSV, were transferred into fibroblasts. We also developed an in vitro shRNA verification method with a target gene expression vector. Results: shRNA activity was confirmed in vitro with vectors that expressed the 3D and ORF7 genes in the cells. Cells containing shRNAs showed lower transcript levels than cells with only the expression vectors. The shRNAs were integrated into CD163-edited cells to combine the two techniques, and the viral genes were suppressed in these cells. Conclusion: We established a multi-resistant strategy against viral diseases and an in vitro shRNA verification method.

• 한국응용곤충학회지
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• 제14권3호
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• pp.147-153
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• 1975

의약용 Capsuel에 넣은 입제형 살충제 BPMC, Carbofuran, Cartap, Chlorphenamidine-HCl, Cytrolane, Diazinon의 단일회 수도근부처리로서 수도 전생육기간에 걸친 이화멸충, 멸구, 매미충류 및 Virus 병의 방제효과를 검정하기 위해 포장시험을 실시하여 아래와 같은 결과를 얻었다. (1) 이화명충은 발생이 적었기 때문에 약제간에 큰 차이는 볼 수 없었지만 약제처리후 50일에서 이화명충 1화기 심고경율 Cartap 0, Carbofuran 0.1, Chlorphenamidine 0.4, Cytrolane 0.5, BPMC 1.8, Diazinon 2.2이었으며 2화기 백수율은 BPMC에서 $1.2\%$이었을 뿐 그밖에 약제처리구에서는 백수가 없었다. (2) 끝동매미충과 애멸구에 대해서는 BPMC와 Diazinon 처리구에서 비교적 낮은 밀도를 보였다. 벼멸구에 대해서는 BPMC와 Carbofuran에서 서식밀도가 현저히 낮아 그 방제효과가 좋았으나 Cartap, Chlorphenamidine, Cytrolane 및 Diazinon의 효과는 거의 기대할 수 없었다. (3) 오갈병의 발병주율은 Carbofuran이 $2.4\%$, Cytrolane $4.5\%$, Chlorphenamidine $7.9\%$, Diazinon $8.0\%$ BPMC$8.1\%$, Cartap $14.5\%$, Diazinon 입제수면 시용 $7.8\%$, 무처리 $13.1\%$로서 Carbofuran 처리구에서 오갈병의 발병율이 가장 낮았다. 줄무늬잎마름병의 발생은 전혀 없었는데 그 원인은 통일벼는 줄무늬잎마름병에 저항성품종이기 때문인 것 같다. (4) 벼의 수량은 무처리 5,814kg/ha에 비하여 Carbofuran 6,649kg, Cytrolane 6,340kg, BPMC 6,315kg, Chlorphenamidine 6,265kg, Cartap 6,164kg, Diazinon 5,944kg, Diazinon 입제 수면시용 6,102kg로서 Carbofuran 처리구에서 벼의 수량이 가장 높았다. (5) Carbofuran 처리구에서 높은 수량을 보인 것은 Carbofuran 처리구에서 멸구, 매미충류의 밀도가 낮았고 오갈병의 발병율이 낮은데서 온 결과인 것 같다.

• 융합보안논문지
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• 제21권1호
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• pp.25-31
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• 2021

본 연구의 목적은 코로나19 바이러스 유행 시기에 교대근무체계로 운영하는 보안관제센터를 무중단으로 유지하기 위한 연구 이다. 사이버 보안위협에 대응하는 보안관제 시설은 24시간 365일 실시간으로 운영해야 하는 필수 보안시설이며, 보안운영 및 관리적인 부분에서 매우 중요하다. 만약 감염병 유행, 시스템 장애, 물리적 영향 등 보안관제 시설이 폐쇄되거나 영향이 있는 경우 실시간 사이버 보안위협에 대응 할 수 없으며, 보안문제에 치명적이 될 수 있다. 최근 코로나19 바이러스 유행으로 인한 시설 폐쇄, 장마철로 인한 보안시스템 가용성 장애 등 보안관제 시설 운영을 할 수 없는 사례가 확인되고 있으며, 이 외에도 물리적 영향으로 보안관제 시설을 운영 할 수 없는 상황에 대한 대비가 필요하다. 본 논문에서는 보안관제 시설을 다중화 시설로 구성하여 폐쇄되는 상황 발생 시 무중단으로 운영 할 수 있는 방안을 제안한다.

• 한국초지조사료학회지
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• 제28권3호
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• pp.221-228
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• 2008

본 시험은 사료용 옥수수 검은줄오갈병 피해 다발지역의 발병실태를 조사하여 종합적인 방제기술 개발에 필요한 기초 자료를 확보하고자, 2006년부터 2007년까지 제주도를 제외한 전국을 대상으로 수행하였다. 검은줄오갈병의 발생은 지역에 따라 차이가 많았으며, 발병이 심한 지역은 경기(이천), 전북(고창, 김제), 전남(영광), 경북(김천, 영주), 충남(천안, 보령)이었다. 옥수수 검은줄오갈병 다발지역에서 파종시기가 4월 26일$\sim$5월 15일까지 파종이 발병이 적었으며, 파종기가 4월 10일$\sim$20일 또는 5월 31일 이후에서 발병이 많았다. 검은줄오갈병의 발생은 옥수수 품종간의 차이는 다소 있으나 저항성을 나타내는 품종은 없었다. 옥수수 검은줄오갈병 감염에 따른 건물수량은 발병률이 높을수록 유의적으로 감소하였으며, 발병률과 옥수수 건물수량 간에는 고도의 부의상관(r=－0.86$^{**}$)이 인정되었다. 검은줄오갈병에 감염된 옥수수는 건전한 옥수수보다 건물소화률은 3.2%, 가소화양분총량은 3.6%, RFV는 12% 낮았으며, NDF와 ADF 함량은 각각 3.7, 4.5% 높아져 전체적으로 사료가치가 낮아지는 것으로 나타났다.

• 식물병연구
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• 제27권3호
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• pp.120-127
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• 2021

식물 바이러스는 작물 수확량에 상당한 손실을 일으키고 작물 생산을 지속적으로 위협하여 세계 식량 안보에 심각한 위협이 된다. 그 중 tomato spotted wilt virus (TSWV)는 주로 원예작물을 감염시키는 가장 위협적인 식물 바이러스로 넓은 기주 범위를 가진다. Reverse-transcription quantitative real-time PCR (RT-qPCR)은 TSWV의 민감한 검출을 위해 널리 사용되고 있지만 표준화의 어려움으로 인해 유용성이 감소한다. 따라서 본 연구에서는 TSWV 검출을 위해 민감하고 정확한 reverse transcription droplet digital polymerase chain reaction (RT-ddPCR)을 확립하였다. TSWV 검출에 대한 RT-qPCR 및 RT-ddPCR의 민감도를 비교하였고, TSWV에 대한 RT-ddPCR의 특이성 분석은 고추에서 주로 발생하는 바이러스 및 음성 대조군에서 특이성을 확인한 결과 증폭되지 않았다. RT-ddPCR 및 RTqPCR에 의해 측정된 TSWV의 선형회귀곡선은 모두 높은 선형성을 나타냈지만, RT-ddPCR 분석이 10배 이상 더 민감하고 더 낮은 TSWV의 copy 수를 검출할 수 있었다. 종합적으로, 우리의 연구 결과는 RT-ddPCR이 TSWV 검출에 대해 높은 민감도와 특이성을 제공하고 낮은 농도의 현장 시료에서 TSWV 검출하는 데 적합하다는 것을 보여준다.

• The Plant Pathology Journal
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• 제40권1호
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• pp.59-72
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• 2024

A comprehensive survey of mungbean-growing areas was conducted to observe leaf spot disease caused by Alternaria alternata. Alternaria leaf spot symptoms were observed on the leaves. Diversity of 50 genotypes of mungbean was assessed against A. alternata and data on pathological traits was subjected to cluster analysis. The results showed that genotypes of mungbean were grouped into four clusters based on resistance parameters under the influence of disease. The principal component biplot demonstrated that all the disease-related parameters (% disease incidence, % disease intensity, lesion area, and % of infection) were strongly correlated with each other. Alt a 1 gene that is precisely found in Alternaria species and is responsible for virulence and pathogenicity. Alt a 1 gene was amplified using gene specific primers. The isolated pathogen produced similar symptoms when inoculated on mungbean and tobacco. The sequence analysis of the internal transcribed spacer (ITS) region, a 600 bp fragment amplified using specific primers, ITS1 and ITS2 showed 100% identity with A. alternata. Potato virus X (PVX) -based silencing vector expressing Alt a 1 gene was constructed to control this pathogen through RNA interference in tobacco. Out of 50 inoculated plants, 9 showed delayed onset of disease. Furthermore, to confirm our findings at molecular level semi-quantitative reverse transcriptase polymerase chain reaction was used. Both phenotypic and molecular investigation indicated that RNAi induced through the VIGS vector was efficacious in resisting the pathogen in the model host, Tobacco (Nicotiana tabacum). To the best of our knowledge, this study has been reported for the first time.