• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2019년도 추계학술대회
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• pp.78-78
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• 2019

The aim of the study was to determine the antioxidant activities of the plants with origin of Vietnam. The Embelia scandens (Lour.) Mez which is a species of plant in the family Myrsinaceae and Cornus hongkongensis Hemsl., which is a species of plant in the family Cornaceae were tested for antioxidant activities. Samples were prepared using 95% ethanol using DPPH assay for assessing the antioxidant activity. Ascorbic acid was used for positive control for DPPH assay. DPPH assay experiment showed that extracts of the Embelia scandens (Lour.) Mez and Cornus hongkongensis Hemsl. might have anti-oxidant activity 4.77 times and 5.65 times higher, respectively, compared to control. To determine the cell toxicity, MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay was used. MTT assay experiment showed that Embelia scandens (Lour.) Mez might have 13.1% more toxicity whereas Cornus hongkongensis Hemsl. might have 47.3% less toxicity compared to control. Taken together, these experiments showed that Cornus hongkongensis Hemsl. extracts might have significantly higher antioxidant activities and relatively lower toxicity, compared to control.

• 치과방사선
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• 제25권2호
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• pp.423-435
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• 1995

The purpose of this study was to observe the effect of TGF-βl, which promotes differentiation and proliferation of osteoblasts, on bone regeneration. Experimental bone defects that measured 3 mm in diameter were created on the mandibles of guinea pig by removal of bone with the use of trephine burs. In one side of mandibular body, the experimental groups, bone defects were grafted with Biogran(Orthovita Co., U.S.A) and TGF-β1(R&D System Co., U.SA). In the remaining side of the mandiblar body, the control groups, bone defects were grafted with only Biogran. Guinea pigs in the control and experimental groups were serially terminated by fours on the 3 days, the 1 week, the 2 weeks, the 3 weeks, and the 4 weeks after experiment, and both sides of the mandibular bodies were removed and fixed with 10％ neutral formalin. They were decalcified and embedded in paraffin as using the usual method. The specimen sectioned and stained with hematoxylin and eosin. Also, they were radiographed with a soft X -ray apparatus. The obtained results were as follows; 1. Hemorrhagic condition, observed in the granulation tissues, disappeared on the 1 week after experiment in both groups, and more prominent in the experimental group. The granulation tissues of the experimental group had larger number of cells than those of the control group. 2. Osteoblastic differentiation in the margin of grafted material and adjacent bone was observed on the 1 week after experiment in both groups. Also, bone formation was observed in immature form on the 1 week after experiment. and more prominent in the experimental group. 3. In the polarizing microscopic examination, bone matrix was very loose on the 1 week after experiment, but increase in density with time, and more prominent in the experimental group. 4. In the microradiographic examination, newly formed bone was observed in the experimental group on the 2 weeks after experiment, and this was observed earlier than in the control group. Newly formed bone was increased with time and defected area was markedly decreased on the 4 weeks after experiment.

• 한국토양비료학회지
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• 제35권6호
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• pp.372-380
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• 2002

표고 750m인 고랭지에서 마사토의 일종인 척박한 모재토를 채취하여 1.5m로 두텁게 성토한 포장조건에 인산증시, 유기물 및 석회질비료 등을 혼용하였을 때 토양화학성분 함량의 변화와 수량성을 검토하기 위하여 1999년에서 2001년까지 3년간 포장시험으로 수행하였다. 유기물, 유효인산 및 치환성칼륨 등 화학성분 함량이 매우 낮은 양질사토인 토양에 감자 "수미"와 배추 "고랭지 여름배추"를 여름재배하였다. 연차의 경과에 따른 토양의 유효인산과 치환성칼륨 함량은 인산 및 칼리질비료의 증비로 증가하였으며, 특히 3년차에서 현저히 증가한 반면, 유기물 함량은 완만한 증가를 나타내었다. 작물 재배지에 모재토(C horizons) 성토시는 매년 표준량의 퇴비연용 및 석회중화량 시용(감자는 더뎅이병 발생 경감을 위하여 1/2량 시용)과 병행하여 토양검정에 의한 인산(흡수계수 5% 조절량) 및 칼리(포화도 5% 조절량)를 증비하는 화학성 개량만으로도 2~3년내 조기 숙전화가 가능함을 알았다. 토양의 유효인산과 치환성칼륨 함량의 증가가 컸던 화학성 개량구의 2~3년차 수량증가율은 표준구에 비하여 감자 5~22%, 배추 6~25% 이였다. 감자 및 배추의 3년차 건물중은 표준구에 비하여 모두 높았고, 생육도 전반적으로 양호한 결과로 나타났다.

• 한국콘텐츠학회논문지
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• 제19권5호
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• pp.455-463
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• 2019

본 연구는 최종대상자는 56명을 대상으로 실험군 29명, 대조군26명을 배정한 후, 실험군에게 구강안면프로그램을 교육하여 저작기능을 향상 시킴으로써 집단 간 구강위생 및 실천 효과를 확인하고자 하였다. 수집한 자료는 프로그램 효과분석은 프로그램 전-후 차이를 알아보기 위해 Wilcoxon signed rank -test를 실시하였다. 실험군의 평균 사후 $9.57{\pm}1.44$점 이었고, 대조군은 사후 평균은 $8.68{\pm}1.46$점으로 실험군의 OHBI가 대조군에 비해 유의하게 높은 것(p<0.05)으로 나타났다. 실험군의 QHI 점수는 평균 사후 $1.00{\pm}.14$점 이었고, 대조군은 사후 평균은 $1.03{\pm}.23$점으로 실험군의 QHI 대조군에 비해 유의하게 낮은 것으로 나타났으나 이는 통계적으로 유의하지는 않았다. 실험군의 프로그램 만족도는 평균점수는 $4.13{\pm}.17$점 이었고 대조군은 $3.94{\pm}.22$점으로 통계적으로 유의한 것으로 조사되었다. 이상과 같은 결과를 바탕으로 구강안면프로그램을 이용한 구강보건교육의 효과는 집단 간 치면세균막 지수, 구강건강행동지수의 차이가 있는 것으로 나타났다. 구강안면운동프로그램을 저작기능의 향상과 구강위생을 위한 도구로 활용할 가치가 있다고 사료된다.

• Journal of Oral Medicine and Pain
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• 제23권4호
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• pp.309-326
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• 1998

In general, the major causative factor of halitosis is thought to be a sulfated compounds. Clusterin, a sulfated glycoprotein-2(SGP-2), is frequently found in diabetic conditions and cold stress conditions. The same result is werum glucose level to diabeteic and cold stress conditions that founded Clusterin. Therefore, this study was performed to examine Clusterin in the slivary glands under stress conditions before insulin injection I.M. Fourty rats were diveded into 3 groups ; 1) 10 rats of gorup I were selected as a control 2) 15 rats beloning to group II were bathed in cold water for 30 seconds twice a day 3) 15 rats in group III received cold stress and injected I.M. with insulin. The rats were sacrifeced at day 0, 3, 5, 7 and 10 of the experiment and the submandibular glands and parotid galnds were removed. RNAs were purified from the salivary of the salivary glands were subjected to Hematoxillin-Eosin stainings and examined under the light microscope. The obtained results were as follows : 1. With immunohistochemistric method, in normal control goup, Clusterin was moderately stained in the intercalated ductal cell of the submandibualr glands, mild stained in the striated ductal cell of the submandibular glands, heavily stained on the cytoplasm of the intercalated ductal cell in the mucous submandibular glands nad slightrly stained in the intercalated ductal cell of the paroted gland, expressed negativity in the acina cell. 2. With immunohistochemistric method, Clusterin slightly increased in the acina cell of the submandibular glands under stress condition at 3 days after experement, moderately stained at 5 days after experiment so revealed positive response. And hearily in the intercalated ductal cell and mildly lin the acina celluar eytoplasm of the parotid glands under stress condition at 3 days experiment. 3. With immunohistochemistric method, no remarkable differences are found between the normal control group and stress conditioned group that insulin administration was performed before. 4. In the stressor-giving group, Clusterin mRNA was porminently expressed in submandibular gland after 5 days after experiment, in parotid gland after 3 days after experiment, performed in immunoelectrophoresis method. 5. In the insulin-injected nad stressor-giving group, Clusterin mRNA was not observed in all experimental submandibular and parotid gland, performed in immunoelectrophoresis method.

• 한국식물병리학회:학술대회논문집
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• 한국식물병리학회 2003년도 정기총회 및 추계학술발표회
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• pp.106-107
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• 2003

The effect of biological control agent Bacillus sp. (BAC03-3-1, BAC03-3-2, BAC02-4) on pre- and postemergence Sclerotinia rot of perilla (Perilla frutescens var. japonica) caused by Sclerotinia sclerotiorum was determined from greenhouse field trials. The ability of this antagonist to reduce germination of sclerotia of S. sclerotiorum was also evaluated. In the greenhouse, suspension of BAC03-3-1 application as root drench of perilla, which provided as little as 10$\^$7/ cells/ $m\ell$ per gram of soil, significantly increased plant stand in pathogen-infested soil over that in the untreated control. All three isolates reduced the germination of sclerotia of S. sclerotiorum in loamy sand soils in the greenhouse. In loamy sand amended with rice bran the sclerotial germination was inversely correlated (r = -0.79) with perilla stand in the greenhouse. However, a higher rate of bacterial suspension with rice bran(Ig dwt./100g soil) than that applied with bacterial suspensions only was necessary to achieve a comparable reduction in sclerotial germination. In field study, all three isolates added to soil to provide 10$\^$7/ cells/$m\ell$ per gram significantly prevented Sclerotinia rot (73-85％) after 35 days of growth. The isolate BAC02-4, BAC03-3-1 and BAC03-3-2 gave final stands of 65 to 75, 60 to 70, and 55 to 60％, respectively. The addition of rice bran(1 ％) to loamy sand in the field resulted in a 10-fold increase in propagule numbers of the three isolates within 10 days of application.

• Asian-Australasian Journal of Animal Sciences
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• 제16권5호
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• pp.706-713
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• 2003

Two experiments were conducted to evaluate the efficacy of dietary FERMKIT, a commercial toxin binder consisting of probiotic-fermented natural product containing chitin, chitosan and chitosan oligosaccharides ($FERMKITO^{(R)}$, EASY-BIO SYSTEM, Inc., Korea), in binding aflatoxin (AF) and zearalenone (ZEN) and ameliorating their mycotoxicity in meat type ducks. FERMKIT was supplemented to AF contaminated diets (at 120 ppb) at either 0.3 or 0.6% in experiment 1 and to ZEN contaminated diets (at 150 ppb) at 0.6% in experiment 2. In experiment 1 body weight gains were reduced by 37% and mortality was increased by 18% in ducks fed diet contaminated with AF at 120 ppb compared to ducks fed control diet (<10 ppb AF) for the 4-wk experimental period. However, dietary FERMKIT supplementation effectively alleviated overall toxicity induced by AF. The significant treatment-related changes in feather growth, web-toe hemorrhage, leg deformity, liver paleness, organ weights, hematological values and serum biochemical values, as compared to the control, were observed. The FERMKIT supplementation significantly diminished the adverse effects of AF and restored all the parameters measured back (<0.05) toward the control values. These findings indicated that FERMKIT, when added at the levels of 0.3 or 0.6% in the 120 ppb AF diets, could modulate the toxicity of AF with percentage sorption capacity of 52.70% at the level 0.3% and 79.85% at the level 0.6% of the diets (experiment 1). In experiment 2, FERMKIT, when added at 0.6% to the 150 ppb ZEN diets for the 4-wk experimental period, diminished the toxicity as shown by body weight gain, weights of testicles, oviducts, Bursa of Fabricius and cloaca eversion score as compared with the controls (<10 ppb ZEN) and 150 ppb ZEN diet with no added FERMKIT. The findings indicated that FERMKIT could be protective against the effects of ZEN in young growing ducks with percentage sorption capacity of 67.11% as evaluated from toxicity index parameter measured when added at 0.6% of the diets containing 150 ppb ZEN.

• 공업화학
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• 제34권3호
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• pp.327-329
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• 2023

본 연구에서는 Clostridium ljungdahlii 배양에서 유기질소공급물인 tryptone과 sodium tungstate의 배양액 내 농도가 균주 성장과 아세트산, 에탄올 생산에 대한 영향을 확인하기 위한 실험을 수행하였다. 대조군 실험의 배지 조건(0 g/L tryptone)보다 tryptone 2.5 g/L를 투입한 경우 균주 성장이 144.6% 증가하였고, 에탄올 및 아세트산 생산은 각각 8.6%와 36.7% 향상되었다. 대조군 실험에 사용된 배지의 조건(0.01 µM Na₂WO₄·2H₂O)보다 100배인 1 µM Na₂WO₄·2H₂O를 사용한 실험군에서, 균주 성장과 C2 대사산물의 총생산량에는 큰 차이가 없지만, 에탄올 생산 증가하고 아세트산 생산은 감소하여 에탄올/아세트산 생산비가 0.56로 대조군의 0.24에서 크게 증가하였다.

• 한국과학교육학회지
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• 제27권3호
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• pp.169-179
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• 2007

이 연구에서는 가설 연역적 탐구 실험이 학생들의 과학의 본성에 대한 관점 변화에 미치는 효과를 조사하였으며, 그 효과는 학습 전략에 따라 분석되었다. 연구 대상은 중학교 2학년 학생 212명이며 학생들은 실험집단과 통제집단으로 무선 배치되었다. 통제집단의 학생들은 안내된 탐구 실힘을 실시하였으며, 실험집단의 학생들은 가설 연역적 탐구 실험을 실시하였다. 연구 결과 사전 검사에서 실험 집단과 통제 집단은 과학의 본성에 대한 관점이 거의 비슷하게 나타났다. 수업을 실시한 후 실험 집단은 과학의 본성 중에서 관찰의 이론 의존성, 과학적 추론, 가설에 대한 관점이 유의미하게 변화되었다. 실험집단 중에서 피상적 전략을 주로 사용하는 학생들은 과학의 본성 중에서 가설에 대한 관점만이 유의미하게 변화되었다. 반면, 실험집단 중에서 심층적 전략을 주로 사용하는 학생들은 과학의 본성 중에서 관찰의 이론의존성, 과학적 추론, 가설에 대한 관점이 유의미하게 변화되었다.

• Journal of Acupuncture Research
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• 제26권5호
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• pp.77-83
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• 2009

Objectives : We investigated the effects of Mahuang-Chuanwu Pharmacopuncture Treatment in Obese Patients by analysing Body mass index(BMI), waist measurement and weight. Methods : We investigated on 52 volunteers consisted of 24 subjects in experiment(Mahuang-Chuanwu Pharmacopuncture) group and 28 subjects in control(Normal Saline) group. The Study form was a single-blind and placebo-controlled clinical trial. 24 subjects in experiment group were injected Mahuang-Chuanwu Pharmacopuncture with additional common treatment and 28 subjects in control group were injected Normal Saline with additional common treatment with Dietic Theraphy and Exercise. We measured BMI, waist measurement and weight on 3 times : before 1st session, after first 5 sessions, and after 10 sessions. The SPSS 13.0 for Windows was used to analyze the data and the wilcoxon signed ranks test (in group), mann Whitney u t-test(between two groups) and ${\chi}^2$-test(description of number) were used to verify the result. Results : In experiment group. BMI, waist measurement and weight were significantly low at the period of after first 5 sessions and after 10 sessions. In control group, BMI, waist measurement and weight were significantly low at the period of after 10 sessions but weren't significantly low at the period of after first 5 sessions. The changes of BMI, waist measurement and weight in experiment group were significantly more than that in control group. Conclusions : The Results suggest that Mahuang(Ephedra sinica Stapf)-Chuanwu(Aconitum carmichaeli Debx) Pharmacopuncture is effective on the treatment of obesity, compared to Normal Saline.