• Title/Summary/Keyword: Continuous feeding

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Wrist joint analysis of Myoelectronic Hand using Accelerometer (가속도계를 이용한 전동의수의 손목관절 시스템 해석)

  • 장대진;김명회;양현석
    • Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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    • 2003.05a
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    • pp.876-881
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    • 2003
  • This study focused on to design and toanalysis of a myoelectronic hand. We considered a low frequency factor in human life and to quantify low frequency which a human body responded to using a 1-axis ant a 3-axis accelerometer. The dynamic myoelectronic hand are important for tasks such a continuous prosthetic control and a EMG signal recognition, which have not been successfully mastered by the most neural approached To control myoelectronic hand, classifying myoelectronic patterns are also important. Experimental results of FEM are 110㎫ on Thumb, 200㎫ on Index finger, 220㎫ on Middle finger 260㎫ on Ring finger and 270㎫ on Little finger. Experimental results of accelerometer are 1.4-0.4(m/s2) ,(5-20(〔Hz〕) in Feeding activity and 0.4-0(m/s2) (0-10〔Hz〕) in Lifting activity. Considering these facts, we suggest a new type myoelectronic hand.

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Production of Recombinant Hirudin in Galactokinase-deficient Saccharomyces cerevisiae by Fed-batch Fermentation with Continuous Glucose Feeding

  • Srinivas Ramisetti;Kang, Hyun-Ah;Rhee, Sang-Ki;Kim, Chul-Ho
    • Biotechnology and Bioprocess Engineering:BBE
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    • v.8 no.3
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    • pp.183-186
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    • 2003
  • The artificial gene coding for anticoagulant hirudin was placed under the control of the GAL 10 promoter and expressed in the galactokinase-deficient strain (Δgal1) of Saccharomyces cerevisiae, which uses galactose only as a gratuitous inducer in order to avoid its consumption. For efficient production of recombinant hirudin, a carbon source other than galactose should be provided in the medium to support growth of the Δgal1 strain. Here we demonstrate the successful use of glucose in the fed-batch fermentation of the Δgal1 strain to achieve efficient production of recombinant hirudin, with a yield of up to 400 mg hirudin/L.

Continuous Production of Pullulan by Aureobasidium pullulans HP-2001 with Feeding of High Concentration of Sucrose

  • Seo Hyung-Phil;Jo Kang-Ik;Son Chang-Woo;Yang Jae-Kyoon;Chung Chung-Han;Nam Soo-Wan;Kim Sung-Koo;Lee Jin-Woo
    • Journal of Microbiology and Biotechnology
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    • v.16 no.3
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    • pp.374-380
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    • 2006
  • In this study, glucose, sucrose, and dextrin were found to be better carbon sources for the production of pullulan by Aureobasidium pullulans HP-2001. Maximal production of pullulan with 200 g/l sucrose as a carbon source was 54.2 g/l. The highest yield of pullulan from sucrose was 0.40, when the sugar concentration was 100 g/1. Optimal conditions for the continuous production of pullulan by A. pullulans HP-2001 in a 7-1 bioreactor were determined by studying the effects of composition of feed solution, dilution rate, and concentration of sucrose in the feed solution. Pullulan concentration and productivity with 100 g/l glucose and 2.5 g/l yeast extract were 38.1 g/l and 0.53 g/l h for 72 h, respectively, in a batch culture of A. pullulans HP-2001. When the substituted medium contained 100 g/l sucrose, 2.5 g/l yeast extract, and mineral salts, which is the same composition as the medium for the production of pullulan, the pullulan concentration and productivity were 74.9 g/l and 0.55 g/l h for 120 h, respectively. The production of pullulan at the steady state increased with a dilution rate up to 0.015/h, and its concentration was 78.4 g/l with a weight average molecular weight ($M_w$) of $4.0{\times}10^5$. Unlike a batch culture, however, the decline of the $M_w$ and the number average molecular weight ($M_n$) of pullulan was not found in the continuous culture of A. pullulans HP-2001. When the concentration of sucrose in the feed solution was 200 g/l, 113.5 g/l of pullulan was obtained at the steady state. The steady state was maintained longer in the continuous culture fed with the feed solution containing 200 g/l sucrose than when fed with the feed solutions containing either 100 or 150 g/l sucrose.

Effect of Continuous Feeding of Probiotics on Growth Performance, Nutrient Digestibility, Blood Urea Nitrogen and Immune Responses in Pigs (지속적인 생균제의 첨가가 돼지의 성장, 영양소 이용율, 혈중 요소태 질소 및 면역능력에 미치는 영향)

  • Kil, D.Y.;Lim, J.S.;Tian, J.Z.;Kim, B.G.;Kim, K.S.;Kim, Y.Y.
    • Journal of Animal Science and Technology
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    • v.46 no.1
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    • pp.39-48
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    • 2004
  • This experiment was conducted to investigate the effect of continuous feeding of probiotics on growth performance, nutrient digestibility, blood urea nitrogen(BUN) and immune responses in pigs. Treatments were 1) Control(basal diet), 2) P-O.l(basal diet + 0.1% probiotics) and 3) P-0.2(basal diet + 0.2% probiotics). In growth trial, a total of sixty pigs(6.17 $\pm$ 0.45 kg average body weight) weaned at 21 days of age were used. All pigs were assigned according to sex and body weight, and each treatment had 5 replicates of 4 pigs per pen in a randomized complete block(RCB) design. During 0${\sim}$8 weeks, there was no significant difference in average daily gain(ADG), average daily feed intake(ADFI) and gain:feed ratio(GfF) among treatments. During 9 - 20 weeks, ADG was improved significantly in pigs fed P-O.I or P-0.2 diets when compared to the pig fed control diet(P <0.05), but there was no significant difference in ADFI and GfF ratio. During overall period, ADG, ADFI and GfF ratio were not significantly different among treatments. In the first metabolic trial(17.93 $\pm$1.45kg average body weight), apparent digestibility of OM, protein, fat in pigs fed P-O.l and P-0.2 diets were greater than in pigs fed control diet(P <0.05) and ash digestibility in pigs fed P-0.2 diet was significantly higher than in pigs fed control diet(P <0.05). Calcium digestibility in pigs fed P-0.2 diet was significantly higher than in pigs fed control and P-O.I diets(P <0.05). Fecal-N excretion was lower in pigs fed P-O.! and P-0.2 diets than in pigs fed control(P <0.05). In the second metabolic trial(41.80 $\pm$ 2.68kg average body weight), there was no significant difference among treatments in apparent digestibility of nutrients and N-retention. In blood assay for the BUN and immune responses investigations, there was no significant difference among treatments during overall period of experiment. Therefore, this experiment suggested that probiotics supplementation could improve growth performance and nutrient digestibility of pigs.

The Reactive Power Compensation for a Feeder by Control of the Power Factor of PWM Converter Trains (PWM 컨버터 차량의 역률 제어를 통한 급전선로의 무효전력 보상)

  • Kim, Ronny Yongho;Kim, Baik
    • Journal of the Korean Society for Railway
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    • v.17 no.3
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    • pp.171-177
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    • 2014
  • PWM converter trains exhibit excellent load characteristics in comparison with conventional phase-controlled trains with low power factors, as they can be operated at power factors which are close to unity by means of a voltage vector control method. However, in the case of a high track density or extended feeding, significant line losses and voltage drops can occur. Instead of operating these trains at a fixed unity power factor, this paper suggests a continuous optimal power factor control scheme for each train in an effort to minimize line losses and improve voltage drops according to varying load conditions. The proposed method utilizes the steepest descent algorithm targeting each car in the same feeding section to establish the optimized reactive power compensation levels that can minimize the reactive power loss of the feeder. The results from a simulation of a sample system show that voltage drops can be improved and line losses decreased.

EFFECT OF SUPPLEMENTATION WITH PROTEIN MEAL ON THE GROWTH OF CATTLE GIVEN A BASAL DIET OF UNTREATED OR AMMONIATED RICE STRAW

  • Perdok, H.B.;Leng, R.A.
    • Asian-Australasian Journal of Animal Sciences
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    • v.3 no.4
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    • pp.269-279
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    • 1990
  • A 98 d feeding trial carried out to study liveweight change and rumen metabolites in heifers weighing initially 275 kg and given either untreated or ammoniated rice straw supplemented with 0, 0.4, 0.8 or 1.2 kg protein meal consisting of cottonseed meal (60). All 32 animals received 0.6 kg rice polishings/hd/d and had continuous access to molasses/urea block-licks containing 15% urea. The effects on growth rates of treatment of the straw with ammonia and of supplementation with bypass protein were additive. The heifers fed ammoniated straw grew 267 g/hd/d (p<0.001) faster and consumed 11% (p<0.05) more straw than the heifers on untreated straw. The mean growth response to bypass protein was 0.37 kg gain/kg protein meal supplied. Supplementation with protein meal tended (p=0.06) to depress intake of straw, but straw intakes of the unsupplemented groups were high. Small changes in the composition of the block-licks that were fed throughout the feeding trial led to changes in block intake and in intake of untreated straw. Increasing quantities of protein meal fed were associated with linear increase in concentrations of ammonia (p<0.05) and in molar percentages of iso-butyrate (p<0.01), iso-valerate (p<0.01) and valerate (p<0.01) in the rumen fluid of the heifers on a basal diet of untreated straw. However, in the rumen fluid of the heifers given ammoniated straw, the levels of these metabolities were not affected by the quantity of protein meal given.

High Concentrated Spore Production of Bacillus thuringliensis by Fed-Batch Processes (유가식 배양공정에 의한 Bacillus thurngiensis의 고농도 포자생산)

  • 박창열;유연우
    • KSBB Journal
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    • v.15 no.3
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    • pp.219-225
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    • 2000
  • Both the production of high spore concentration and high insecticidal activity are required in the production of Bacillus thuringiensis to be used for the bacterial insecticide. In the production of high cell and spore concentrations of B. thuringiensis the continuous fed-batch culture(CFBC) and intermittent fed-batch culture(IFBC) were investigated at $28^{\circ}C$ by maintaining 40% dissolved oxygen concentration. When the final glucose concentration was 50 g/L the maximum viable cell number obtained using the CFBC with linear gradient feeding was $9.37{\times}109$ cells/mL and maximum spore concentration was $8.33{\times}109$ spores/mL which was approximately 84.4% yield of spore formation. When the final glucose concentration was 100 g/L the aximum viable cell and spore concentrations obtained using the IFBC with pH-statb were $1.38{\times}$1010 cells/mL and $1.35{\times}1010$ spores/mL respectively and the yield of spore formation was approximately 97.8%.

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NIR PREDICTIONS OF INDIRECT RESPONSES

  • Claridge, Rovert-Burling;Ross Clarke
    • Proceedings of the Korean Society of Near Infrared Spectroscopy Conference
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    • 2001.06a
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    • pp.3103-3103
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    • 2001
  • NIR has been extensively used to predict directly measurable properties of materials that are important to the appropriate industries. Commonly, NIR is used to perform fast, routine tests to improve control response as against the response time for the (normally chemical) base test. This paper discusses the use of NIR to measure indirect properties of materials. In these cases, the pure chemical or physical tests are either unable measure the appropriate parameters (eg GMO modification) or there are mitigating effects that are not properly addressed by the base tests. In particular, we looked at the digestible portion of amino acids within meat and bone meal. This is the desired response measurement by end-users of the product (intensive livestock producers) but is currently unable to be offered as a measurement by producers. The base test method is by controlled feeding trials. These are somewhat cumbersome, taking 2-3 months, involving several sets of animals, and considerable expense. A shortened test (feed trial based) would be of little use, as the precision blows out over short period feeding trials. For example, a rat ileal digestibility test requires around 2 months, and costs some $USD1000. This is clearly impractical test for a producer involved in continuous production, with a 1-2 day turn around. While the amino acid abundance is accessible chemically, the uptake of amino acids into usable material by mammalian species is not simply related to the measured abundance within the material. There are many co-related material properties that might help or hinder uptake, some chemical based (eg protein damage), some indirect (eg palatability), some physiological (intestinal tract response vs speed of throughput). We discuss the approaches taken to provide a suitable reference data set, and present the derived prediction and validation relationships.

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Effect of Rice-Bundle size the harvesting performance with binder and the threshing performance (볏단크기의 변화가 바인더에 의한 수확작업과 탈곡작업에 미치는 영향)

  • 김성래;안수봉;김기대
    • Journal of Biosystems Engineering
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    • v.5 no.1
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    • pp.51-57
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    • 1980
  • It is desirable to increase the diameter of rice bundle harvested by Japanese reaper binder recently introduced into Korean farmers , since it is too small for stalking in the field for preliminary drying prior to threshing operation which is dominant procedure in rice harvesting . Accordingly, this study was conducted to analyze the effect of the size of rice-bundle on the performance of binder and self-feeding thresher. The results are summarized as follows : 1. The diameter of rice-bundle could be increased from $\phi$80-98 to $\phi$105.0-125.4 while the number of rice bundles per 10 a were reduced to 1200 from 1600. The time required for harvesting 10 a of rice was 81 minutes in small size bundles and 84 minutes in large size bundles and no statistical difference was obtained. 2 .The grain loss due to discharge and cutting were slightly increased with large size bundle compared to the small size but no statistical difference was obtained. The precision of operation and drying rate was not significantly different between small and large size bundles. 3. The unthreshed losses were the same between large and small sizes of rice bundles when self-feeding thresher was used. When 8 PS engine was used , continuous operation was possible for small size bundles , but 1.5seconds of time interval was necessary for large size buldles. 4. The consumption of binding twine was reduced to 603 from 820 meters with the larger bundles , and the labour requirement for stalking rice bundles in the field was also reduced to 1.83 form 2.50 man-hour per 10 a Therefore, harvesting cost can be reduced up to 26.5 percent by increasing the bundle size.

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Studies on Cultural Characteristics for High Density Fermentation of Phellinus linteus WI-001 (Phellinus linteus WI-001 균사체의 고밀도 배양을 위한 배양학적 특성 연구)

  • 김종래;권호균;전계택;김규중;이계관
    • Microbiology and Biotechnology Letters
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    • v.28 no.2
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    • pp.105-110
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    • 2000
  • Various environmental factors such as pH, temperature and initial glucose concentration were investigated for enhancing cell growth in fermentations of Phellinus linteus WI-OOl, a producer of polysaccarides with potent anticancer activities. Optimal pH and temperature were around 5.5 and $28^{\circ}C$, respectively. Relatively little variation of pH was observed ranging between 5.5 and 6.5 during the whole fermentation period. Maximum cell concentration and specific growth rate were investigated in the media containing initial glucose concentrations of 0.5%, 1 %, 2%, 3% and 4%. High initial glucose concentration enhanced biomass production but showed negative effect on specific growth rate. In bioreactor experiments with various feeding strategies, increases of 28% and 42% in final cell concentration were obtaind as compared to conventional batch process, by adopting pulse and continuous supplement of 2% glucose solution, respectively.

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