• Title/Summary/Keyword: Content of chlorophyll

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Changes of Characteristics Related to Photosynthesis in Soybean Leaves Infected with Pseudomonas syringae pv. glycinea (세균성 점무늬병에 감염된 콩의 광합성 관련 특성 변화)

  • Ryu, Kyung-Yul;Heu, Hoon
    • Korean Journal Plant Pathology
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    • v.11 no.1
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    • pp.61-65
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    • 1995
  • Photosynthetic characteristics of soybean leaves infected with Pseudomonas syringae pv. glycinea were investigated for 8 days. The difference in photosynthesis rate between healthy and diseased soybean leaves decreased for 2 to 4 days after inoculation and then increased. In respiration rate, healthy and diseased leaves showed the same tendency as photosynthetic rate. The stomatal resistance increased following Pseudomonas syringae pv. glycinea infection. The total chlorophyll content of the infected leaf was less than that of the uninfected. Pseudomonas syringae pv. glycinea infection induced the malformation of stacked grana in chloroplast. Dry matter production declined after infection.

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The development of non-agricutural chemical leaf perilla using Arabidopsis non-specific lipid transfer proteins (AtnsLTP) (Arabidopsis non-specific lipid transfer proteins (AtnsLTP) 처리에 의한 무농약 기능성 잎들깨 생산기술)

  • 허상선;김학윤;유선균;김경민
    • Journal of Life Science
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    • v.13 no.5
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    • pp.576-581
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    • 2003
  • Recombinant Arabidopsis non-specific lipid transfer proteins (nsLTPs) was purified from yeast. In order to determine the effect of nsLTPs for an production of anthocyanin in perilla leaves, ‘Manchudlggae’ cultivar was grown at pots that had been applied with different concentration of nsLTPs. The anthocyanin content in AtnsLTP treated leaves increased above two-fold higher than that in control. Also chlorophyll content was increased 16%. It was presumed that AtnsLTPs could be applied to increase high quality of perilla leaves.

Effects of Aluminum Solution Treatment on the Growth of Forsythia koreana Cuttings (알루미늄용액 처리가 개나리삽수의 생장에 미치는 영향)

  • 김갑태
    • Korean Journal of Environment and Ecology
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    • v.6 no.1
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    • pp.9-11
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    • 1992
  • To examine aluminum toxicity on woody plants, Forsythia koreana cuttings were grown in the aluminum solution and ground water(pH 6.75). Aluminum solution were prepared 1.0, 2.5 and 5.0mM aluminum potassium sulfate, dilulted with ground water. Shoot growth, leaf number, leaf injury and leaf chlorophyll content were measured and compared among the treatments. In all growth-related characters(shoot growth. leaf number, leaf injury and leaf chlorophyll content), differences among the treatments were highly significant. Forsythia koreana cuttings were severely stressed in aluminum solution more than 1.0mM concentration.

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Physiological Responses of Green Roof Plants to Drought Stress (건조스트레스에 따른 옥상녹화 식물의 생리적 반응)

  • Park, Seong-Sik;Choi, Jaehyuck;Park, Bong-Ju
    • Journal of the Korean Institute of Landscape Architecture
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    • v.45 no.2
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    • pp.51-59
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    • 2017
  • This study evaluated the drought tolerance of Liriope platyphylla F.T.Wang & T.Tang, Dendranthema zawadskii var. lucidum (Nakai) J.H.Park, Hosta longipes (Franch. & Sav.) Matsum., Sedum sarmentosum Bunge and Zoysia japonica Steud. for an extensive green roof. In order to assess drought tolerance of green roof plants, several criteria were measured such as volumetric water content, leaf and soil moisture potential, chlorophyll a and b, chlorophyll fluorescence, photosynthesis, stomatal conductance, transpiration rate and antioxidants. The results of the drought tolerance measurement of green roof plants focused on the gradually withering of plants from lack of volumetric water content. D. zawadskii was the first to show an initial wilting point, followed by Z. japonica, H. longipes and L. platyphylla in order while S. sarmentosum showed no withering. It was concluded that H. longipes, L. platyphylla and S. sarmentosum were highly drought tolerant plants able to survive over three weeks. Furthermore, chlorophyll a and b were divided into two types: Type I, which kept regular content from the beginning to the middle of the period and suddenly declined, like H. longipes and Z. japonica; and Type II, which showed low content at the beginning, sharply increased at the middle stage and decreased, like D. zawadskii, L. platyphylla and S. sarmentosum. Volumetric water content and the amount of evapotranspiration consistently declined in all plant species. The analysis of chlorophyll fluorescence results that S. sarmentosum, which had relatively high drought tolerance, was the last to decline, while Z. japonica and S. sarmentosum withered after rapid reduction. At first, photosynthesis, stomatal conductance and transpiration rate showed high activity, but they lowered as the plant body closed stomata owing to the decrease in volumetric water content. Measuring antioxidants showed that when drought stress increased, the amount of antioxidants grew as well. However, when high moisture stress was maintained, this compound was continuously consumed. Therefore, the variation of antioxidants was considered possible for use as one of the indicators of drought tolerance evaluation.

Contents of Carotenoids and Chlorophylls in Dolsan Leaf Mustard(Brassica juncea) (돌산갓의 Carotenoids 및 Chlorophyll 함량)

  • Cho, Young-Sook;Ha, Bong-Seuk;Park, Seok-Kyu;Chun, Soon-Sil
    • Journal of the Korean Society of Food Culture
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    • v.8 no.2
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    • pp.153-157
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    • 1993
  • To furnish basic data for the utilization of leaf mustard as a raw material of salted and fermented vegetable food, the contents of carotenoids and chlorophylls of Dolsan Leaf Mustard(DLM) were investigated. Total carotenoid content of DLM was 4.75 mg%, and the compositions were ${\beta}-carotene(80.91%)$, lutein(13.07%), lutein epoxide(3.93%). The contents of chlorophyll a and b were 4.1 and 1.5 mg%, and leaf was 7.4- and 8.1-fold, respectively, higher than leaf stalk. The ratios of chlorophyll a/b in leaf (2.7:1) and leaf stalk(3.0:1) were similar to those of other vegetables.

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Comparative transcriptome analysis of heat stress responsiveness between two contrasting ginseng cultivars

  • Jayakodi, Murukarthick;Lee, Sang-Choon;Yang, Tae-Jin
    • Journal of Ginseng Research
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    • v.43 no.4
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    • pp.572-579
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    • 2019
  • Background: Panax ginseng has been used in traditional medicine to strengthen the body and mental well-being of humans for thousands of years. Many elite ginseng cultivars have been developed, and ginseng cultivation has become well established during the last century. However, heat stress poses an important threat to the growth and sustainable production of ginseng. Efforts have been made to study the effects of high temperature on ginseng physiology, but knowledge of the molecular responses to heat stress is still limited. Methods: We sequenced the transcriptomes (RNA-Seq) of two ginseng cultivars, Chunpoong (CP) and Yunpoong (YP), which are sensitive and resistant to heat stress, respectively, after 1- and 3-week heat treatments. Differential gene expression and gene ontology enrichment along with profiled chlorophyll contents were performed. Results: CP is more sensitive to heat stress than YP and exhibited a lower chlorophyll content than YP. Moreover, heat stress reduced the chlorophyll content more rapidly in CP than in YP. A total of 329 heat-responsive genes were identified. Intriguingly, genes encoding chlorophyll a/b-binding proteins, WRKY transcription factors, and fatty acid desaturase were predominantly responsive during heat stress and appeared to regulate photosynthesis. In addition, a genome-wide scan of photosynthetic and sugar metabolic genes revealed reduced transcription levels for ribulose 1,5-bisphosphate carboxylase/oxygenase under heat stress, especially in CP, possibly attributable to elevated levels of soluble sugars. Conclusion: Our comprehensive genomic analysis reveals candidate loci/gene targets for breeding and functional studies related to developing high temperature-tolerant ginseng varieties.

Using Chlorophyll(SPAD) Meter Reading and Shoot Fresh Weight for Recommending Nitrogen Topdressing Rate at Panicle Initiation Stage of Rice

  • Nguyen, Hung The;Nguyen, Lan The;Yan, Yong-Feng;Lee, Kyu-Jong;Lee, Byun-Woo
    • Journal of Crop Science and Biotechnology
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    • v.10 no.1
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    • pp.33-38
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    • 2007
  • Nitrogen management at the panicle initiation stage(PI) should be fine-tuned for securing a concurrent high yield and high quality rice production. For calibration and testing of the recommendation models of N topdressing rates at PI for target grain yield and protein content of rice, three split-split-plot design experiments including five rice cultivars and various N rates were conducted at the experimental farm of Seoul National University, Korea from 2003 to 2005. Data from the first two years of experiments were used to calibrate models to predict grain yield and milled-rice protein content using shoot fresh weight(FW), chlorophyll meter value(SPAD), and the N topdressing rate(Npi) at PI by stepwise multiple regression. The calibrated models explained 85 and 87% of the variation in grain yield and protein content, respectively. The calibrated models were used to recommend Npi for the target protein content of 6.8%, with FW and SPAD measured for each plot in 2005. The recommended N rate treatment was characterized by an average protein content of 6.74%(similar to the target protein content), reduced the coefficient of variation in protein content to 2.5%(compared to 4.6% of the fixed rate treatment), and increased grain yield. In the recommended N rate treatments for the target protein content of 6.8%, grain yield was highly dependent on FW and SPAD at PI. In conclusion, the models for N topdressing rate recommendation at PI were successful under present experimental conditions. However, additional testing under more variable environmental conditions should be performed before universal application of such models.

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Effect of Silicon on Growth and Temperature Stress Tolerance of Nephrolepis exaltata 'Corditas'

  • Sivanesan, Iyyakkannu;Son, Moon Sook;Soundararajan, Prabhakaran;Jeong, Byoung Ryong
    • Horticultural Science & Technology
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    • v.32 no.2
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    • pp.142-148
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    • 2014
  • Effect of silicon (Si) nutrition on growth and temperature stress tolerance of Nephrolepis exaltata 'Corditas' grown in a soilless substrate was examined. In vitro-grown acclimatized plantlets were transplanted into the pots containing a coir-based substrate. A nutrient solution containing 0, 50, or $100mg{\cdot}L^{-1}$ Si was supplied through a drip-irrigation system. After 5 months of cultiv ation, S i-treated and -untreated p lants were grown at 10, 25, or $40{\pm}1^{\circ}C$ under a 12 h photoperiod with $530{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ PPFD and 60% RH. After 7 days, chlorophyll content and chlorophyll fluorescence parameters were measured. Silicon nutrition had a negative effect on growth characteristics of N. exaltata 'Corditas'. However, Si-treated plants had more tolerance to temperature stress than the control plants. The Fv/Fm value was not significantly different when the plants were exposed to $25^{\circ}C$. However, significant difference in Fv/Fm was recorded when plants were exposed to 10 or $40^{\circ}C$. Thus, Fv/Fm could be used as an indicator of low and high temperature tolerance in ferns. The present study also suggests that application of Si may be used to enhance temperature tolerance of ferns.

Applicability of DGCI (Dark Green Color Index) to Assess Potential Impacts of CO2 Leakage from the Geological Storage Site (이산화탄소 지중저장 시설의 잠재적 누출 판단을 위한 DGCI(Dark Green Color Index) 적용 가능성 평가)

  • Yoo, Sin Yee;Song, Yoon Jin;Oh, Hee Joo;Kim, You Jin;Yoo, Ga Young
    • Journal of Climate Change Research
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    • v.7 no.3
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    • pp.351-356
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    • 2016
  • The carbon capture and storage (CCS), which collects and stores carbon dioxide in a geological site, is a promising option to mitigate climate change. However, there is the possibility of carbon dioxide leakage from the soil in the steps of collecting, transporting, and storing. To ensure the feasibility of this technology, it is important to monitor the leakage of carbon dioxide and to assess the potential impacts. As plants are sensitive to the changes in carbon dioxide in the soil environment, we can utilize plant parameter to detect the carbon dioxide leakage. Currently, chlorophyll a content is a conventional index indicating the changes in plants, however, this method is labor intensive and it only utilizes a small portion of leaves. To overcome its limitations, a simple spectroscopic parameter, DGCI (dark green color index), was suggested as an easy and quick indicator. In this study, we compared the values of chlorophyll a contents with DGCI from the experiment investigating the impacts of high underground $CO_2$ on grape plants. Results suggest that DGCI had high correlation with chlorophyll a contents and it has high potential to be utilized as an easy indicator to monitor plants' responses to $CO_2$ treatment.

Changes of Chloroplast-Mediated Electron Transport Activity and Chlorophyll-Protein Complexes in Barley Seedlings by Decursinol (Decursinol 처리에 따른 보리 유식물의 전자전달 활성과 엽록소-단백질 복합체의 변화에 대하여)

  • 이현식
    • Journal of Plant Biology
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    • v.31 no.2
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    • pp.131-141
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    • 1988
  • The effects of decursinol and decursin on chloroplast-mediated electron transport and phosphorylation in barley seedlings were investigated in comparison with coumarin in the dark or light. The changes of CP-complexes were also studied. Decursinol, decursin and coumarin caused marked inhibitory effects on germination of seed and electron transport and phosphorylation activity of seedlings. The following order of inhibitory effectiveness was exhibited; decursinol>coumarin>decursin. Loss of chlorophyll and decrease of electron transport activity were retarded in the dark, but were reversely accelerated in the light by these three chemicals. The changes of CP-complex patterns were also similar to effects on chlorophyll content and the electron transport activity. These opposite effect in the dark and light suggest that these three chemicals act as natural growth retardants rather than cytokinins or growth inhibitors.

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