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Production of Minor Gisenosides from Gypenoside V (Gypenoside V로부터 minor ginsenosides의 생산)

  • Son, Na-Ri;Min, Jin-Woo;Jang, Mi;Kim, Hyo-Yeon;Jeon, Ji-Na;Yang, Deok-Chun
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2010.10a
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    • pp.20-20
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    • 2010
  • Panax ginseng C.A Meyer is frequently taken orally as a traditional herbal medicine in Asian countries. The major components of ginseng are ginsenoside, which are pharmaceutical activity. The six major ginsenosides, including Rb1, Rb2, Rc, Rd, Re and Rg1 account for 90% of total ginsenosides. Even though the minor ginsenosides, including Rg3, Rh2 and compound K has high pharmacetical activities, the price of minor ginsenosides is too high. Therefore we isolated the gypenoside V and made it converted to minor ginsenosides. In the plant Gynostemma pentaphyllum Makino, gypenosdie V was presented as dominant saponin (content about 2.4%), and was similar to protopanaxadol type ginsenosides such as ginsenoside Rb1. In this study, we confirmed that the coversion of gypenoside V to minor ginsenosides after using the various treatment such as heating, acid treatment, commercial edible enzyme, and lactobacillus. Consequently, we optimizied the transformation of gypenoside V to minor ginsenoside using Thin Layer Chromatography (TLC), High Performance Liquid Chromatography (HPLC), Time-of-flight Mass Spectrometry (LC/TOF/MS).

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Studies on the Adhesion of W to TiN(l) (TiN 에 대한 W의 부착특성에 관한 연구(l))

  • Lee, Jong-Mu;Gwon, Nan-Yeong;Son, Jae-Hyeon;Kim, Hyeong-Jun
    • Korean Journal of Materials Research
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    • v.3 no.5
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    • pp.466-475
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    • 1993
  • Adhesion of W to TiN which is used as a glue layer in the blanket tungsten process has been investigated using the pulling and scratch methods and analysed using surface roughness measurements by SEM and the reflectivity measurement, the stress measurement and the SIMS depth profiling Significant differences between the adhesion of the CVD tungsten film to the s-TiN and that to the a-TiN were found. It appeared that the fomer were better than the latter, which is due to the facts that the s-TiN film has a rougher surface and a lower stress level and a higher oxygen content than the a-TiN film. Also the ashesion strength was degreaded with increasing the TIN film thickness because of the increase of the TiN film stress.

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The Fabrication of Nickel-Diamond Composite Coating by Electroplating Method (전기도금방법을 이용한 Ni-Diamond 복합도금층 제조에 대한 연구)

  • Moon, Yun-Sung;Lee, Jae-Ho;Oh, Tae-Sung;Byun, Ji-Young
    • Journal of the Microelectronics and Packaging Society
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    • v.14 no.1
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    • pp.55-60
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    • 2007
  • The codeposition behavior of submicron sized diamond with nickel from nickel electrolytes has been investigated. Electroplating of diamond dispersed nickel composites was carried out on a rotating disk electrode (RDE). The effects of current type and current density on the electrodeposited Ni-diamond composite coating were investigated. The effects of surfactants on the composite coating were also investigated. The hardness of coating was measured with varying electroplating conditions using Micro Vickers. As diamond was incorporated into the coating, the hardness of coating as well as the wear resistance was improved. The hardness of the coating was increased as much as 100% and the wear resistance was improved as much as 27%. The hardness of composite coating layer increased slightly at the diamond content of above 20 gpl.

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Error Concealment Method Based on POCS for Multi-layered Video Coding (다계층 비디오 코딩에 적용 가능한 POCS 기반 에러 은닉 기법)

  • Yun, Byoung-Ju
    • Journal of the Institute of Electronics Engineers of Korea SP
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    • v.46 no.4
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    • pp.67-75
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    • 2009
  • Multi-layered video coding that provides scalability across the visual content has emerged for easily adaptive service over current heterogeneous network. However, the network is still error prone environment so that video service may suffer packet loss or erroneous decoding of the video. Especially distortion caused by the burst error may propagate to several pictures until intra refreshing, which will raise a terrific degradation of picture quality. To overcome the problem at terminal independently, we propose a new error concealment algorithm for the multi-layered video coding. The proposed method uses the similarity of between layers in the multi-layered video coding and POCS (Projections Onto Convex Sets) which is a powerful error concealment tool, but heavily dependent on initial values. To find adequate initial value which can reduce iteration times as well as achieve high performance, we took consideration into both features of layered approach coding and the correlation in neighbor blocks. The simulation results show that the proposed concealment method works well.

Comparison of IgE induction in mice by pollens from three pine tree species

  • Kim, Seo-Yoong;Oh, In-Bo;Choi, Kee-Ryong
    • Journal of Ecology and Environment
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    • v.41 no.9
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    • pp.265-270
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    • 2017
  • Background: Over the years, pine pollens have been excluded as an allergen due to its relatively large size, low protein content, and waxy hydrophobic layer, despite their abundance. However, recent studies suggest the possibilities of pine pollens being allergens, and it has been reported that allergy symptoms were highly prevalent in areas with considerably large pine forests and high possibility of exposure to the pollen. Therefore, we conducted a comparative analysis of the allergenicities of the pollens from the dominant species of Korean pines, red pine (Pinus densiflora), black pine (Pinus thunbergii), and pitch pine (Pinus rigida), in mice. Methods: The protein composition of the pollens from the three pine species was compared via sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). The pine pollens and proteins extracted from the pollens were introduced to BALB/c mice by nasal inhalation and application to exposed skin and the IgE produced by the mice were extracted from blood and analyzed via ELISA. Results: SDS-PAGE showed differing protein compositions of the pollens of the three pine species. Analysis of blood IgE compositions showed a similar amount of IgE produced when pollens were applied to skin. In contrast, when mice inhaled the pollens, P. densiflora was shown to induce significantly more IgE production than those of the other two species. Conclusions: The experimental results demonstrate that the pollens of all three South Korean pine species induce IgE production, and this production was more pronounced when the pollens were inhaled than when they were applied to the skin. Of the three species, the pollen of P. densiflora was found to induce the highest level of IgE production.

Quality Monitoring of Specification of Crataegi Fructus in the Korean Pharmacopoeia and Studies HPLC Standard Chromatogram (산사(山楂)의 규격 기준 모니터링 및 HPLC 표준크로마토그램 연구)

  • Kim, Kyoung Hee;Kim, Sun Mi;Lee, Young Jong;Baek, Wan Sook
    • The Korea Journal of Herbology
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    • v.32 no.1
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    • pp.55-62
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    • 2017
  • Objectives : Crataegi Fructus has been used as traditional medicines for more digestion action, amenorrhea due to blood stasis, and hyperlipemia. The aim of this study was to compare of Crataegi Fructus in South Korea collected during three years according to the standards in monographs of the Korean Pharmacopoeia Eleventh edition (KP 11). Methods : Crataegi Fructus was carried out identification test (Qualitative reaction, Thin layer chromatography), heavy metal test, and total ash registered at KP. Add to we tested loss on dry, contents of ethanol-soluble extracts, and HPLC profiling. Results : Identification test (TLC) was on comparing with ursolic acid standard solution in $R_f$ value, all samples showed red purple spot ($R_f$ value 0.9). Ursolic acid spot in $R_f$ value 0.35 showed by changing mobile phase condition. Heavy metals showed contents for Pb, Cd, As, and Hg range of 0.0 ~ 0.5 ppm, 0.0 ~ 0.2 ppm, 0.0 ~ 0.3 ppm, and 0.0 ~ 0.1 ppm. Loss on drying was ranged from 5.5 to 11.9 %, total ash was between the range 2.7 ~ 4.0 %. Contents of ethanol-soluble extracts was ranged from 17.8 to 44.9 %. The content of chlorogenic acid was ranged from 0.0 to 0.1 % based on the chlorogenic acid standard curve. Conclusion : We have verified the current specification standard of Crataegi Fructus and standard that is not set. We hope that it will help the standardization of Crataegi Fructus.

Erosion Behavior of YAG Ceramics under Fluorine Plasma and their XPS Analysis (불소계 플라즈마에 노출된 YAG 세라믹스의 식각거동 및 XPS 분석)

  • Kim, Kyeong-Beom;Kim, Dae-Min;Lee, Jung-Ki;Oh, Yoon-Suk;Kim, Hyung-Tae;Kim, Hyung-Sun;Lee, Sung-Min
    • Journal of the Korean Ceramic Society
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    • v.46 no.5
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    • pp.456-461
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    • 2009
  • Chemical composition and status of chemical bonding of the YAG($Y_3Al_5O_{12}$) ceramics after the exposure to fluorine plasma have been investigated using X-ray photoelectron spectroscopy, with the analysis on its erosion behavior. On the surface, F showed the maximum content, decreasing with depth, meanwhile the cation composition remained almost constant, irrespective of the position. The peaks due to Y in the reaction layer consisted of two kinds, showing the Y-O and Y-F bonds. These surface modifications under fluorine plasma seem to promote the erosion of the YAG ceramics. Excess addition of $Al_2O_3$ or $Y_2O_3$ into stoichiometric YAG produced 2nd phases of $Al_2O_3$ and $YAlO_3$, respectively, resulting in the slight difference in the local erosion rates. But, the overall average erosion rate was not sensitive to such excess additions of $Al_2O_3$ or $Y_2O_3$.

Antioxidative Materials in Domestic Meju and Doenjang 2. Separation of Lipophilic Brown Pigment and Their Antioxdative Activity (재래식 메주 및 된장중의 항산화성 물질에 관한 연구 2. 지용성 갈변물질의 분리와 항산화력)

  • Kim, Mi-Hye;Im, Sang-Sun;Kim, Sung-Hee;Kim, Gyeong-Eup;Lee, Jong-Ho
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.23 no.2
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    • pp.251-260
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    • 1994
  • Lipophilic brown pigments produced during the fermentation of domestic Meju and Doenjang were fractionated by column and thin -layer chromatography (TLC). Each of the fractions was tested for the antioxidant activity and then characterized by spectroscopic analysis. The lipophilic brown pigments were separated into chloroform -soluble and methanol-soluble parts in which Meju resulted the higher content of chloroform-soluble part than that of methanol-soluble part ; however, Doenjang exhibited the opposite result to that of Meju. More strong antioxidant activity was found in the methanol-soluble part than the chloroform-soluble part. Four and five fractions were separated from chloroform-soluble and methanol-soluble parts respectively. by silicagel TLC. The fraction that exhibited the high antioxidant activity showed a strong absorption at 260nm caused by amino compounds in UV spectrum The other fractions which did not have antioxidant activity absorbed at 240nm by carbonic acid and it ester,. IR spectrum of each fraction commonly showed absorption at 3400cm-1 , 2800cm-1 , 1700cm-1, 1600cm-1, 1400cm-1 , 1300cm-1 and 1100cm-1. Especially , the fraction which had a strong antioxidant activity showed absorption at 2800cm-1, 1400cm-1, 1600cm-1 suggesting that the fraction contain Schiff's base and primary amine structure.

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Lyotropic Behaviors of a Phospholipid-based Lamella Liquid Crystalline Phase Hydrated by Propylene Glycol as a Polar Solvent: Correlation of DSPC vs PG Concentration

  • Jeong, Tae-Hwa;Oh, Seong-Geun
    • Bulletin of the Korean Chemical Society
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    • v.28 no.1
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    • pp.108-114
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    • 2007
  • The lyotropic behaviors to form the structure of distearoylphosphatidylcholine (DSPC)-based liquid crystal (LC) hydrated by only propylene glycol (PG) without water were examined by differential scanning calorimetry (DSC), X-ray diffractions (XRD), polarized microscope (PM) and transmission electron microscope (TEM). By increasing the amount of PG instead of water, it showed the phase transition to be gradually changed from anisotropic structures to other structures more close to isotropic ones and their appearance to be changed from solid-like states to liquid-like ones with more fluidity. Below 50% w/w PG, the mixtures of DSPC and PG resulted in no direct observation of LC structure through PM because they were very close to solid-states. From 55% w/w to 90% w/w of PG, the dense lamella crystalline structures were observed through PM, and their thickness and area decreased as the content of PG increased. Measured by DSC with heating process, the main phase transition from α -lamella phase to isotropic phase appeared from 52.89 °C to 47.41 °C to show linearly decreasing behaviors because PG affects the hydrophobic region of DSPC-based lamella phase. The repeating distance of the lamella phase and the interlayer distance between bilayers were calculated with XRDs and the average number of bilayers related to the thickness in LC structure was approximately estimated by combining with TEM results. The WAXS and DSC measurements showed that all of PG molecules contributed to swelling both the lipid layer in the edge region of lamella phase close to phosphate groups and the interlayer between bilayers below 90% w/w of PG. The phase and thermal behaviors were found to depend on the amount of PG used by means of dissolving DSPC as a phospholipid and rearranging its structure. Instead of water, the inducement of PG as a polar solvent in solid-lamella phase is discussed in terms of the swelling effect of PG for DSPC-based lamella membrane.

A study on the Application of PB/MC-CDMA for IoT Services in Small Cell Environment (IoT 서비스를 위한 스몰셀 환경에서 PB/MC-CDMA 적용 방안에 대한 연구)

  • Lee, Kyu-Jin
    • Journal of Convergence Society for SMB
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    • v.6 no.3
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    • pp.21-27
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    • 2016
  • In this paper, we introduce the PB/MC-CDMA (Partial Block/Multi-Carrier-Code Division Multiple Access) system to mitigate inter-cell interference (ICI) and enhance user capacity in the small cell environment. In 5G mobile communications, the number of devices connected to the network is expected to increase exponentially with the expansion of the IoT (Internet of Things) services. In addition, each device is expected to be required by the various data rates by their content types. In LTE/LTE-A, there are some limitations that large scale connectivity and supporting various data rates. Therefore, we introduce a PB/MC-CDMA physical layer system which is suitable for the small cell environment, and evaluate the performance in the multi cell environment which is affected by ICI. Through computer simulation results, we demonstrate the effectiveness of PB/MC-CDMA for the small cell environment.