• KSBB Journal
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• v.23 no.1
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• pp.37-43
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• 2008

The use of antihemophilic factor IX complex has been associated with a variety of thrombotic complications, the major cause of which was the contamination of thrombogenic proteins such as vitamin K-dependent clotting factors II, VII, and X. In order to produce a commercial factor IX (GreenNine VF) free from thrombogenic potential, industrial-scale production process for high-purity factor IX from human plasma has been developed. The purification process contains cryo-precipitation, DEAE-sephadex A-50 anion-exchange chromatography, DEAE-toyopearl 650M anion-exchange column chromatography, heparin-sepharose 6FF affinity column chromatography, and CM-sepharose FF cation-exchange column chromatography. Also the process includes two viral inactivation and removal procedures, solvent/detergent treatment and nanofiltration using Viresolve NFP filter. The purification yield was 35.4%. The specific activity in the purified concentrate was 190.8 IU/mg which exceeded that in the factor IX complex (FacNine) by a factor of 48. The activities of factor II, VII, and X were not detected in GreenNine VF. SDS-PAGE analysis showed that GreenNine VF had the highest purity in comparison with commercially available high purity factor IX concentrates, Mononine, Octanyne, Berinin HS, and Immunine STIM plus 600. One batch size of the production was 2,400 vials of 250 IU product or 1,200 vials of 500 IU product from 1,600 L cryo-poor plasma.

• Journal of Korean Society of Environmental Engineers
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• v.31 no.2
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• pp.125-131
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• 2009

Reductive dechlorination of polychlorinated dibenzo-p-dioxins (PCDDs) and its toxicity change were predicted by the linear free energy relationship (LFER) model to assess the zero-valent iron (ZVI) and anaerobic dechlorinating bacteria (ADB) as electron donors in PCDDs dechlorination. Reductive dechlorination of PCDDs involves 256 reactions linking 76 congeners with highly variable toxicities, so is challenging to assess the overall effect of this process on the environmental impact of PCDD contamination. The Gibbs free energies of PCDDs in aqueous solution were updated to density functional theory (DFT) calculation level from thermodynamic results of literatures. All of dechlorination kinetics of PCDDs was evaluated from the linear correlation between the experimental dechlorination kinetics of PCDDs and the calculated thermodynamics of PCDDs. As a result, it was predicted that over 100 years would be taken for the complete dechlorination of octachlorinated dibenzo-p-dioxin (OCDD) to non-chlorinated compound (dibenzo-p-dioxin, DD), and the toxic equivalent quantity (TEQ) of PCDDs could increase to 10 times larger from initial TEQ with the dechlorination process. The results imply that the single reductive dechlorination using ZVI or ADB is not suitable for the treatment strategy of PCDDs contaminated soil, sediment and fly ash. This LFER approach is applicable for the prediction of dechlorination process for organohalogen compounds and for the assessment of electron donating system for treatment strategies.

• Journal of the Korean Society of Food Science and Nutrition
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• v.38 no.5
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• pp.612-617
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• 2009

Fresh vegetable juice is a non-heat treated product and the only step to reduce microbial growth is washing. Therefore, the materials for fresh vegetable juice including Angelica keiskei, Brassica loeracea var. acephala, and Daucus carota L. were treated by ozone after the first washing process and investigated for microbial and chemical changes. The number of the total aerobic bacteria in materials after selection step were $8.2{\times}10^5{\sim}5.0{\times}10^6\;CFU/g$, which was a higher contamination level than the limit of Korea food code ($10^5\;CFU/g$). However, after the 1st washing process and ozone treatment, the total aerobic bacterial number was reduced to $4.7{\times}10^4{\sim}6.7{\times}10^4\;CFU/g$, which showed 2 log microbial reduction. After the 2nd washing step followed by ozone treatment, there was no difference in microbial number. The number of colifroms in the materials of fresh vegetable juice were $8.0{\times}10^3{\sim}3.5{\times}10^3\;CFU/g$ initially but showed $1.5{\times}10^2{\sim}3.0{\times}10^2\;CFU/g$ after the ozone treatment (1 log reduction). On the other hand, there was no changes in the contents of ascorbic acid, flavonoids, polyphenols, minerals (cadmium and lead) during all processes. In addition, no color changes were observed during washing process. Therefore, ozone treatment in the materials of fresh vegetable juice decreased the microbial numbers. Also, chemical characteristics of ozone treated sample were not different when compared with control.

• Clean Technology
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• v.27 no.1
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• pp.33-38
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• 2021

Demand for lithium-based secondary batteries is greatly increasing with the explosive growth of related industries, such as mobile devices and electric vehicles. In Korea, there are several top-rated global lithium-ion battery manufacturers accounting for 40% of the global secondary battery business. Most discarded lithium secondary batteries are recycled as scrap to recover valuable metals, such as Nickel and Cobalt, but residual wastes are disposed of according to the residual lithium-ion concentration. Furthermore, there has not been an attempt on the possibility of water discharge system contamination due to the concentration of lithium ions, and the effluent water quality standards of public sewage treatment facilities are becoming stricter year after year. In this study, the as-received waste water generated from the cathode electrode coating process in the manufacturing of high-nickel-based NCM cathode material used for high-performance and long-term purposes was analyzed. We suggested a facile recycling process chart for waste water treatment. We revealed a correlation between lithium-ion concentration and pH effect according to the proposed waste water of each recycling process through analyzing standard water quality tests and daphnia ecological toxicity. We proposed a realistic waste water treatment plan for lithium electrode manufacturing plants via comparison with other industries' ecotoxicology.

• Journal of the Korea Institute of Building Construction
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• v.3 no.1
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• pp.107-114
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• 2003

This study aims to find methods that prevents aging of buildings paint coating and that limits defects in construction. Defects in painting can occur in four stages: pure paint, during painting, after the paint coating has dried, and after some period of time has passed after coating. Paint may become bad due to precipitation of pigments, formation of membranes, and seeding during manufacturing. Therefore, it is important that the paint is well mixed and kept airtight at a cool, dark place. Indents, paint brush strokes, orange peel, separation of colors, and paint running and spreading during the paint work process can be prevented by using high quality materials and applying a high-level of construction method. After the paint coating has dried, boiling, yellowing, poor drying, poor bonding, and/or glen deficiency may occur. These are influenced by the levels of cleanness of the dried product, drying temperature and hydration. Then, when the coating has been left dried for some period of time, cracking, peeling, scaling, swelling, discoloring, and/or rusting may develop due to the ultraviolet and contaminants in the air. Since these defects occur due to inappropriate construction schedule and/or hot and humid condition, one must use weatherproof materials. Furthermore, poor paint color may be caused by contamination in the sample plate, discoloration, and/or discrepancies in colors which are due to material differences, level of glossiness, degree of dispersion, dual color property of metallic colors, precipitation of pigments, etc. One should achieve reduction in construction cost and effectiveness in paint work by limiting contaminations in the construction site and strictly observing to construction regulations.

• Journal of Soil and Groundwater Environment
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• v.18 no.1
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• pp.78-84
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• 2013

This study was conducted in order to evaluate the removal process for long-term contamination sources including chlorinated hydrocarbons (TCE and PCE) and explosive compounds (TNT, RDX, and HMX) in underground water using a pulsed-UV system. Crystallized cells containing the contaminants were placed 10, 20, and 40 cm away from a lamp that emits pulsed-UV rays in order to examine how the removal efficiency is influenced by the distance between the source of the light and the compounds. Chlorinated hydrocarbons were completely removed in 30 minutes with a distance of 10 cm, while PCE was completely removed even with a distance of 20 cm. In the case of explosive compounds, removal efficiencies slightly varied depending on the compounds. The majority of the compounds were perfectly removed with a contact time of 10 minutes. In particular, for RDX, the results showed that complete removal was obtained within one minute, regardless of the distance from the UV source. The amount of light energy is in inverse proportion to the distance, and thus the energy reaching the compounds severely diminishes as the distance increases. Therefore, the removal efficiency decreased with increasing distance in the system.

• Journal of Korean Society of Water and Wastewater
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• v.12 no.1
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• pp.45-51
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• 1998

The determinations of ammonia in water for drinking purpose served as one basis of judging the sanitary quality of water for a great many years. However, presently ammonia regulation varies depending on countries. In USA and Canada, ammonia is added to water for chloramination process. However, for korea, there is ammonia regulation of treated water in Korea which should not exceed 0.5mg/l as $NH_3-N$. There was a report exceeding 0.5mg/l of ammonia in chlorinated water when the famous drinking water contamination episode happened in the downstream of Nadong River, 1994. With lack of sewer distribution system and treatment plants of domestic wastes, many water treatment plants have a difficulty of complying with ammonia regulation in treated water. Breakpoint chlorination is usually performed to get rid of ammonia. The method which is allowed to measure ammonia in Korea is Phenate method. However, it would be undesirable to use Phenate method for measuring ammonia in chlorinated water if Phenate method would not differentiate ammonia from chloramine. A good possibility of interferences in measurement of ammonia exists because Phenate method include the step of the formation of chlorine and would not differentiate chloramine which is formed as a result of reaction between chlorine and ammonia. This study was on inaccuracy of Phenate method for measuring ammonia of chlorinated water when chloramine and ammonia coexist. This study found that Phenate method measured all chlormaine as ammonia. Ammonia measurement by ion chromatography confirmed this results. Finally, the result from this study suggests that ammonia measurement by Phenate method in chlorinated water should be revised accordingly.

• Korean Journal of Materials Research
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• v.22 no.4
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• pp.202-206
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• 2012

$CoSi_2$ was formed through annealing of atomic layer deposition Co thin films. Co ALD was carried out using bis(N,N'-diisopropylacetamidinato) cobalt ($Co(iPr-AMD)_2$) as a precursor and $NH_3$ as a reactant; this reaction produced a highly conformal Co film with low resistivity ($50\;{\mu}{\Omega}cm$). To prevent oxygen contamination, $ex-situ$ sputtered Ti and $in-situ$ ALD Ru were used as capping layers, and the silicide formation prepared by rapid thermal annealing (RTA) was used for comparison. Ru ALD was carried out with (Dimethylcyclopendienyl)(Ethylcyclopentadienyl) Ruthenium ((DMPD)(EtCp)Ru) and $O_2$ as a precursor and reactant, respectively; the resulting material has good conformality of as much as 90% in structure of high aspect ratio. X-ray diffraction showed that $CoSi_2$ was in a poly-crystalline state and formed at over $800^{\circ}C$ of annealing temperature for both cases. To investigate the as-deposited and annealed sample with each capping layer, high resolution scanning transmission electron microscopy (STEM) was employed with electron energy loss spectroscopy (EELS). After annealing, in the case of the Ti capping layer, $CoSi_2$ about 40 nm thick was formed while the $SiO_x$ interlayer, which is the native oxide, became thinner due to oxygen scavenging property of Ti. Although Si diffusion toward the outside occurred in the Ru capping layer case, and the Ru layer was not as good as the sputtered Ti layer, in terms of the lack of scavenging oxygen, the Ru layer prepared by the ALD process, with high conformality, acted as a capping layer, resulting in the prevention of oxidation and the formation of $CoSi_2$.

• Korean Journal of Plant Resources
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• v.32 no.6
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• pp.730-734
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• 2019

Cryopreservation is one of the ideal and suitable methods for long-term storage of plant germplasm. The plant contaminated with diseases and pathogens are decreased the multiplication rate, survival rate and high quality of plants after cryopreservation. The aim of this work was to improve a micropropagation method for lily in Korea, which is indigenous plant. In the last process of rinsing scales after surface-sterilization, we tried to control the diseases and pathogens lived within the tissue by rinsing in 0.03% sodium hypochlorite (NaClO) instead of sterile distilled water. Bulb scales of Lilium were cultured in vitro on MS medium supplemented with Plant Preservative Mixture (PPM). The results showed that L. tsingtauense accessions were observed ranged from 53.9 to 100% with a mean value of 76.8% and L. hansonii accessions were checked from 84.5 to 85.5% with a mean of 85% survival rate. The newly small bulb formed from bulb-scales was transferred to MS medium. We checked the presence of microorganisms and survival rate after 3 weeks in culture after examination of bacterial incidences. The results indicated that the non-contamination rate were shown ranged from 75.0 to 94.1% with mean value of 83.2% in L. tsingtauense species, and that L. hansonii were observed 85.1 to 91.7% with mean value of 88.4%. This study will provide a valuable basis for establishment of effective axenic cultures for in vitro micropropagation of Korean native lily species.

• Journal of the Korean Institute of Rural Architecture
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• v.21 no.4
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• pp.45-52
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• 2019

Chemical grouting method is mainly used for construction of dams and reservoirs, stabilization and reinforcement of slopes, reinforcement of soft grounds such as embankments, dredging and landfills, the order of earthquake response method, and the reinforcement of structures. Recently, it is widely applied in construction sites such as highways, airfields, high-speed railways, subsea facilities, port construction works, tunnels, and subway works. As such, the demand for grouting continues to increase. The development of the grouting method was focused on increasing the strength of the ground, and the development of the chemical additives, the injection device, and the stirring device were mainly performed. But ordinary portland cement used for grouting is a product that consumes natural resources such as limestone, generates a large amount of greenhouse gases, consumes a large amount of energy sources, and it is time to develop products and new methods to replace them. In this study, Ordinary Portland Cement and New Grouting Binder (circulating fluidized bed boiler fly and blast furnace slag) were compared and analyzed by the following test. Homo-gel strength and homo-gel time, water quality analysis of the water used and soil contamination process tests of homo-gel samples were performed. In the case of NGB, when Using water is used as the reservoir water, the strength measured smaller than that of the other water. However, it shows about 2.5 times greater than the homo-gel compressive strength applied to OPC (7-day, reservoir water), so there is no problem with water quality when applied.