• 한국동물위생학회지
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• 제35권4호
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• pp.289-294
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• 2012

Mycoplasmas are highly fastidious bacteria, difficult to culture and slow growing. Infections with Mycoplasma species can cause a variety of problems in living organisms and in vitro cell cultures. In this study, we investigated the usefulness of a genus-specific consensus PCR analysis method to detect Mycoplasma species. The developed consensus primer pairs MycoF and MycoR were designed specifically to amplify the 16S ribosomal RNA gene (rRNA) of Mycoplasma species by the optimized PCR system. The developed consensus PCR system effectively amplified 215 bp of Mycoplasma genus-specific region of 16S rRNA. In conclusion, we recommend this consensus PCR for monitoring Mycoplasma species in animals, human and cell culture system.

• Communications for Statistical Applications and Methods
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• 제12권2호
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• pp.335-348
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• 2005

The rapid development of microarray technologies enabled the monitoring of expression levels of thousands of genes simultaneously. Recently, the time course gene expression data are often measured to study dynamic biological systems and gene regulatory networks. For the data, biologists are attempting to group genes based on the temporal pattern of their expression levels. We apply the consensus clustering algorithm to a time course gene expression data in order to infer statistically meaningful information from the measurements. We evaluate each of consensus clustering and existing clustering methods with various validation measures. In this paper, we consider hierarchical clustering and Diana of existing methods, and consensus clustering with hierarchical clustering, Diana and mixed hierachical and Diana methods and evaluate their performances on a real micro array data set and two simulated data sets.

• 기본간호학회지
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• 제12권2호
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• pp.162-170
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• 2005

Purpose: This study was conducted to develop a curriculum model for fundamentals of nursing at the graduate level which reflects a consensus of educators and the students in Korean nursing society. Method: A survey was used with three groups to identify consensus on a curriculum model for fundamentals of nursing at the graduate level; 42 educators in fundamentals of nursing, 11 nurse educators whose major was not fundamentals of nursing and 70 nurses with a bachelor degree in nursing. Results: Consensus on a curriculum model at the graduate level were delineated from analysis of the survey, Based on these results, a curriculum model fur fundamentals of nursing at the graduate level is outlined: As one major area of nursing, fundamentals of nursing focuses on nursing practice based on basic human needs. Thus main focus of the curriculum for fundamentals of nursing at the graduate level is research performance and theory development on nursing interventions to meet basic human needs in clinical settings. Conclusions: A curriculum model for fundamentals of nursing at the graduate level suggests that its application may have a positive impact on development of distinct knowledge body for fundamentals of nursing that will differentiate the content of education for undergraduate and graduate levels of nursing. However, the suggestion for the curriculum model needs to be refined and developed for application.

• BMB Reports
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• 제44권4호
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• pp.238-243
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• 2011

We generated 16,993 expressed sequence tags (ESTs) from two libraries containing full-length cDNAs from the brain and liver of the Korean Jindo dog. An additional 365,909 ESTs from other dog breeds were identified from the NCBI dbEST database, and all ESTs were clustered into 28,514 consensus sequences using StackPack. We selected the 7,305 consensus sequences that could be assembled from at least five ESTs and estimated that 12,533 high-quality single nucleotide polymorphisms (SNPs) were present in 97,835 putative SNPs from the 7,305 consensus sequences. We identified 58 Jindo dog-specific SNPs in comparison to other breeds and predicted seven synonymous SNPs and ten non-synonymous SNPs. Using PolyPhen, a program that predicts changes in protein structure and potential effects on protein function caused by amino acid substitutions, three of the non-synonymous SNPs were predicted to result in changes in protein function for proteins expressed by three different genes (TUSC3, ITIH2, and NAT2).

• 한국약용작물학회:학술대회논문집
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• 한국약용작물학회 2010년도 심포지엄 및 추계학술발표회
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• pp.215-216
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• 2010

• 한국동물위생학회지
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• 제38권1호
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• pp.31-36
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• 2015

Two cats were obtained from a cat kennel. Over the previous 7 days, the cats had shown cough, anorexia, depression and nasal discharge. In this study, the consensus PCR was able to detect successfully Mycoplasma species in nasal swab samples of the cats. To identify feline mycoplasma species from the lung tissue of the cats with pneumonia, Mycoplasma species-specific PCR reactions were conducted. As the results, we could identify M. felis by the positive amplified DNAs. On the other hand, we could not detect any positive reactions with the PCR reaction for M. arginini, M. canis, M. edwardii, M. cynos, M. gateae, M. maculosum, M. molared, M. opalescens, M. spumans and Mycoplasma HRC-689. In conclusion, we detected M. felis from the kenneled cats with pneumonia. We suggested that this consensus PCR would be useful and effective for monitoring Mycoplasma species in various kinds of animals including cats. The application of preceding consensus PCR before the species-specific PCRs may be the most recommended strategy for the identification of Mycoplasma spp.

• Child Health Nursing Research
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• 제30권2호
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• pp.142-153
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• 2024

Purpose: This study aimed to describe the process of developing a validated pediatric nursing simulation scenario template using the real-time Delphi method. Methods: A panel of 13 pediatric nursing experts participated in a real-time Delphi survey conducted over two rounds. Initially, 83 items were included in the questionnaire focusing on the structure and content of the simulation scenario template. Data analysis involved calculating the content validity ratio (CVR) and the coefficient of variation to assess item validity and stability. Results: Through iterative rounds of the Delphi survey, a consensus was reached among the experts, resulting in the development of a pediatric nursing simulation scenario template comprising 41 items across nine parts. The CVR values ranged from 0.85 to 1.0, indicating a high consensus among experts regarding the inclusion of all items in the template. Conclusion: This study presents a novel approach for developing a pediatric nursing simulation scenario template using real-time Delphi methods. The real-time Delphi method facilitated the development of a comprehensive and scientifically grounded pediatric nursing simulation scenario template. Our template aligns with the International Nursing Association for Clinical Simulation and Learning standards, and provides valuable guidance for educators in designing effective simulation scenarios, contributing to enhanced learning outcomes and better preparation for pediatric clinical practice. However, consideration of cultural and contextual adaptations is necessary, and further research should explore alternative consensus criteria.

• 한국환경보건학회지
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• 제50권2호
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• pp.81-82
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• 2024

Urban air mobility (UAM) is emerging as an innovative transportation solution for cities. However, the potential noise impact on urban life must be carefully examined. Continuous exposure to UAM noise, with its unique frequency characteristics and temporal variability, may adversely affect citizens' health by causing sleep disorders, cardiovascular disease, and cognitive impairmenet, particularly in children. NASA has formed a UAM Noise Working Group to study this issue comprehensively. In Korea, the Seoul Metropolitan Government's UAM demonstration project is expected to accelerate related research and development. Scientific analysis, including noise measurement, prediction modeling, and health impact assessment, must be prioritized. Measures to minimize noise should be established based on this evidence, such as optimizing flight modes, developing noise reduction technologies, and establishing new noise management standards. Transparency and social consensus are crucial throughout this process. Expert review and open communication with civil society are necessary to address related concerns. Sharing demonstration project results and providing opportunities to experience UAM noise through digital twin simulations can help address public concerns and build social consensus. Proactively and scientifically tackling noise issues is essential for the sustainable development and successful integration of UAM into daily life.

• International Journal of Stem Cells
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• 제16권1호
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• pp.16-26
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• 2023

Despite long-term research achievements, the development of cell therapy (CT) products remains challenging. This is because the risks experienced by the subject and therapeutic effects in the clinical trial stage are unclear due to the various uncertainties of CT when administered to humans. Nevertheless, as autologous cell products for systemic administration have recently been approved for marketing, CT product development is accelerating, particularly in the field of unmet medical needs. The human experience of CT remains insufficient compared with other classes of pharmaceuticals, while there are countless products for clinical development. Therefore, for many sponsors, understanding the rationale of human application of an investigational product based on the consensus and improving the ability to apply it appropriately for CT are necessary. Thus, defining the level of evidence for safety and efficacy fundamentally required for initiating the clinical development and preparing it using a reliable method for CT. Furthermore, the expertise should be strengthened in the design of the first-in-human trial, such as the starting dose and dose-escalation plan, based on a sufficiently acceptable rationale. Cultivating development professionals with these skills will increase the opportunity for more candidates to enter the clinical development phase.

• 인터넷정보학회논문지
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• 제21권1호
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• pp.17-31
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• 2020

블록체인 기술이 급속도로 발전하고 있음에 따라 금융·물류 등 다양한 분야에서 블록체인 기술을 실용화하려는 시도들이 진행되고 있으며, 데이터 무결성이 매우 중요한 공공분야 또한 마찬가지이다. 국방분야 또한 네트워크 중심 작전환경(NCOE) 하에 작전운영을 위해서는 지휘통신 네트워크의 보안성 강화 및 완전무결성 확보가 매우 중요하다. 이를 위해 블록체인 네트워크를 적용한 지휘통신네트워크 구축이 필요하나, 현재까지의 블록체인 기술은 51% 공격 등의 보안 이슈들을 해결하지 못하고 있어, 국방에 접목하기 어려운 것이 현실이다. 특히, 현재 블록체인에서 많이 사용되고 있는 Practical Byzantine fault tolerance (PBFT)알고리즘은, 악의적인 행동을 하는 노드들에게 penalty 요소가 없고, 합의를 방해하는 노드가 전체 노드의 33%이상만 차지해도 합의 실패를 만드는 문제점이 있다. 본 논문에서는 블록체인의 주요 합의 알고리즘인 PBFT의 보안성 향상을 위해, Trust 모델을 접목하여 비정상 행위에 대한 penalty 메커니즘이 적용된 Adaptive Consensus Bound PBFT (ACB-PBFT) 합의 알고리즘을 제안한다.