• Title/Summary/Keyword: Congo red

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Multifarious activities of cellulose degrading bacteria from Koala (Phascolarctos cinereus) faeces

  • Singh, Surender;Thavamani, Palanisami;Megharaj, Mallavarapu;Naidu, Ravi
    • Journal of Animal Science and Technology
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    • v.57 no.7
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    • pp.23.1-23.6
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    • 2015
  • Cellulose degrading bacteria from koala faeces were isolated using caboxymethylcellulose-Congo red agar, screened in vitro for different hydrolytic enzyme activities and phylogenetically characterized using molecular tools. Bacillus sp. and Pseudomonas sp. were the most prominent bacteria from koala faeces. The isolates demonstrated good xylanase, amylase, lipase, protease, tannase and lignin peroxidase activities apart from endoglucanase activity. Furthermore many isolates grew in the presence of phenanthrene, indicating their probable application for bioremediation. Potential isolates can be exploited further for industrial enzyme production or in bioremediation of contaminated sites.

Investigation for culture conditions and characteristics of crude protein-bound polysaccharides compositions extracted from Agaricus blazei Murill

  • Park, Young-Hyun;Hong, Eock-Kee
    • 한국생물공학회:학술대회논문집
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    • 2005.04a
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    • pp.99-102
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    • 2005
  • The Basidomycetes fungus Agaricus blazei Murill has been well known as a health food for the prevention of cancer, diabetes, hyperlipidemia, arteriosclerosis and chronic hepatitis. This study was concentrated to investigate the characteristics of crude protein-bound polysaccharides(PBP) compositions extracted from Agaricus blazei Murill. In order to produce crude polysaccharides, culture conditions were examined using YMK media. Total sugars and protein contents of PBP were detected by phenol-sulfuric acid method and Bradford -assay. Hexosamine was found to be involved in the linkage, N-linked and O-linked types. To identify helical conformation existence, wavelength was measured using Congo red after the treatment with alkali solution.

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Decolorization of synthetic dyes by Rhodopseudomonas palustris P4

  • Oh, You-Kwan;Kim, Yeon-Hee;Park, Sung-Hoon
    • 한국생물공학회:학술대회논문집
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    • 2003.04a
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    • pp.403-408
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    • 2003
  • A newly isolated Rhodopseudomonas palustris P4 could decolorize various synthetic dyes containing different chromogenic groups such as azo linkage (Crocein Orange G, New Coccine, Chromotrope FB, Congo Red, Remazol Black B), anthraquinone Reactive blue 2, or indigo Indigo Carmine. Among them, the degradation rate of Black B was studied in detial. Degradation of Black B followed the Arrhenius equation in 25 - $40^{\circ}C$ with an activation energy of 7.79 kcal/mol. Optimum pH was 8. Glucose in the range of 5 - 50g/l did not affect the Black B decolorization. When Black B increased from 25 mg/l to 2000 mg/l, decolorization activity increased almost linearly but the extent of decolorization was constant at about 86% irrespective of dye concentration. Analyses by HPLC revealed that the Black B molecules were partially degraded and some chromogenic intermediates were produced. These results indicate that Rps. palustris P4 has an outstanding capability to degrade various dyes.

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Two Cases of Primary Localized Amyloidosis of Larynx (원발성 국소 후두아밀로이드증 2례)

  • 김형태;조승호;전범조;김민식
    • Journal of the Korean Society of Laryngology, Phoniatrics and Logopedics
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    • v.9 no.2
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    • pp.147-151
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    • 1998
  • Primary localized laryngeal amyloidosis is an uncommon disorder of unknown cause that occurs in the absence of systemic amyloidosis or associated disease. There is a risk of either missing concomitant systemic amyloidosis or exhaustively investigating for this when it is not present through failure to appreciate the nature of the disease. We present 2 cases of primary localized laryngeal amyloidosis in supraglottic region. Biopsy of the mass of patients revealed findings consistent with amyloidosis, which were Congo red reaction with a apple green birefringence in polarized light fluorescence microscopy. An extensive workup for systemic amyloidosis was negative. All of two cases were treated by vaporization via $CO_2$ LASER using "Swiftlase Flshscan" for creating a wide, shallow char-free treatment site by precisely controlling ablation depth without causing residual thermal damage to tissue. The postoperative recovery of all cases was uneventful with good vocal quality and no aspiration. At the present time, the patients have no evidence of disease, recurrence and complication.

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Isolation and Characterization of Soil Streptomyces Involved in 2,4-Dichlorophenol Oxidation

  • Kang, Min-Jin;Kang, Ja-Kyoung;Kim, Eung-Soo
    • Journal of Microbiology and Biotechnology
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    • v.9 no.6
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    • pp.877-880
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    • 1999
  • Over 50 morphologically distinctive soil Streptomyces were isolated from various Jocations in the Yongin area in Korea and visually screened for dye-decoloring activities on an agar plate. Two Streptomyces species (AD001 and ND002) showed strong dye-decoloring activities on the plate containing congo-red and new-fuchin dyes, respectively. Also, the liquid culture supernatants of these species showed 2,4-dicholophenol (DCP) oxidation activities only in the presence of hydrogen peroxide, a characteristic of Actinomycetes lignin-peroxidase (ALiP)-P3 isoform found in dye-degrading S. viridosporus T7A and S. badius 252. Based on their dye-decoloring capabilities and the 2,4-DCP oxidation kinetic data, it is suggested that these Streptomyces secrete not-yet-characterized extracelluar enzyme(s), whose activities are very similar to the ALiP-P3 enzyme.

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Fabrication of Functional Microcapsule for Drug Delivery by using Droplet Phase Flow (Droplet 유동을 이용한 마이크로캡슐의 제작)

  • Jeong, Eun-Ho;Kim, Il;Go, Jeung-Sang;Kim, Kyung-Chun
    • Journal of the Korean Society of Visualization
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    • v.4 no.2
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    • pp.6-10
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    • 2006
  • The functional spherical microcapsules were produced through the innovative conjunction of the well-defined amphiphilic block copolymer and the stable droplet phase flow in the micro chemical plant. The microcapsules were formed to have hollow inner cavity and outer surface wall with nano-pores. To examine the potential of encapsulating foreign biochemical molecules, Congo-red dye was loaded into the microcapsule. The release performance in the specific surroundings such as temperature, pH and time was evaluated quantitatively.

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Ureteral Washing Cytology of Localized Ureteral Amyloidosis - A Case Report- (요관 세척 세포학적 검사로 진단된 요관 아밀로이드증 - 1 예 보고 -)

  • Lee, Won-Ae;Kie, Jeong-Hae;Jeen, Yoon-Mi;Lee, Mi-Kyung
    • The Korean Journal of Cytopathology
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    • v.13 no.1
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    • pp.38-41
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    • 2002
  • Localized amyloidosis of the ureter is rare and clinically confused with neoplasm. We describe a case of localized amyloidosis of the ureter in which the presence of amyloid was detected in ureteral washing cytology. A 75-year-old female presented with gross hematuria. Abdominal CT and retrograde pyelography revealed hydronephrosis and hydroureter on the left side with abrupt narrowing of the distal ureter. Ureteral washing cytology yielded a hypocellular smear with many irregular clumps of amorphous, extracellar, waxy material. Biopsy sections demonstrated submucosal deposits of eosinophilic amorphous material which gave characteristic apple green birefringence with Congo-red stain under the polarized light. Familarity with the cytologic features of amyloid is helpful for preoperative diagnosis and proper treatment.

Laccase Activity and Azo Dye Decolorization Potential of Podoscypha elegans

  • Pramanik, Satadru;Chaudhuri, Sujata
    • Mycobiology
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    • v.46 no.1
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    • pp.79-83
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    • 2018
  • Azo dyes containing effluents from different industries pose threats to the environment. Though there are physico-chemical methods to treat such effluents, bioremediation is considered to be the best eco-compatible technique. In this communication, we discuss the decolorization potentiality of five azo dyes by Podoscypha elegans (G. Mey.) Pat., a macro-fungus, found growing on the leaf-litter layer of Bethuadahari Wildlife Sanctuary in West Bengal, India. The fungus exhibited high laccase and very low manganese peroxidase activities under different culture conditions. Decolorization of five high-molecular weight azo dyes, viz., Orange G, Congo Red, Direct Blue 15, Rose Bengal and Direct Yellow 27 by the fungus was found to be positive in all cases. Maximum and minimum mean decolorization percentages were recorded in Rose Bengal (70.41%) and Direct Blue 15 (24.8%), respectively. This is the first record of lignolytic study and dye decolorization by P. elegans.

[ ${\beta}-Amyloid$ ] Imaging Probes (베타아밀로이드 영상용 프로브)

  • Jeong, Jae-Min
    • Nuclear Medicine and Molecular Imaging
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    • v.41 no.2
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    • pp.112-117
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    • 2007
  • Imaging distribution of ${\beta}-amyloid$ plaques in Alzheimer's disease is very important for early and accurate diagnosis. Early trial of the ${\beta}-amyloid$ plaques includes using radiolabeled peptides which can be only applied for peripheral ${\beta}-amyloid$ plaques due to limited penetration through the blood brain barrier (BBB). Congo red or Chrysamine G derivatives were labeled with Tc-99m for imaging ${\beta}-amyloid$ plaques of Alzheimer patient's brain without success due to problem with BBB penetration. Thioflavin T derivatives gave breakthrough for ${\beta}-amyloid$ imaging in vivo, and a benzothiazole derivative [C-11]6-OH-BTA-1 brought a great success. Many other benzothiazole, benzoxazole, benzofuran, imidazopyridine, and styrylbenzene derivatives have been labeled with F-18 and I-123 to improve the imaging quality. However, [C-11]6-OH-BTA-1 still remains as the best. However, short half-life of C-11 is a limitation of wide distribution of this agent. So, it is still required to develop an Tc-99m, F-18 or I-123 labeled agent for ${\beta}-amyloid$ imaging agent.

Assessment of the Potential Carbon Credits from Reducing Emissions from Deforestation and Enhancement of Forest Carbon Stock Activities in Developing Countries (개도국의 산림전용으로 인한 온실가스 배출량 감축 및 산림탄소축적 증진 활동의 탄소배출권 잠재력 평가)

  • Bae, Jae Soo;Bae, Ki Kang
    • Journal of Korean Society of Forest Science
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    • v.98 no.3
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    • pp.263-271
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    • 2009
  • This study aims to identify negotiation alternatives related to Post-2012 reducing emissions from deforestation (RED) and enhancement of forest carbon stock (EFCS) activities. It also aims to recommend a negotiation strategy considering environmental integrity and national interest on the basis of estimating reduction potentials of each alternative on the assumption that tradable carbon credits play an important role as positive incentives. In order to estimate greenhouse gas (GHG) reduction potentials and income potential from RED and EFCS activities, 99 countries were selected by the Global Forest Resources Assessment of the Food and Agriculture Organization of the United Nations. A 'baseline and credit' method was applied to estimate RED activities. Gross-net and net-net methods were applied for EFCS activities. According to the results, Brazil, Indonesia, and the Democratic Republic of Congo have more potential to get positive incentives through RED, while China, Chile, and the Republic of Korea have more potential to get positive incentives through EFCS. This study suggests including both RED and EFCS activities in the boundary of policy approaches and endowment of positive incentives to consider GHG reduction potentials in the global scale and equity among developing countries. Making a discount rate application of forest management activities can be also recommended to factor out the effects of human-induced activities by EFCS activities.