• Korean Journal of Pharmacognosy
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• 제19권1호
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• pp.28-33
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• 1988

To identify biologically active enzymatic components of the higher fungi in Korea, the dried carpophores of Cryptoderma citrinum was smashed with cool distilled water, extracted, salted out and the precipitate was purified by dialysing with visking tube and dissolved with pH 7.8 ammonia aqua. The fraction of the filtrate was lyophilized to obtain as light brown powder and then cellulase activity was determined. Cellulolytic potency of Cryptoderma citrinum was 750 unit/g. The optimum condition for the enzymatic reaction was pH 4.5 and $40^{\circ}$. The enzyme activity was activated in the presence of $Ca^{2+}$, $Fe^{2+}$ and $Co^{2+}$.

• Journal of Aquaculture
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• 제19권1호
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• pp.40-46
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• 2006

Bacillus subtilis strain with highly active chitosanase was isolated from the intestine of Sebastiscus marmoratus (scorpion fish). It was named as Bacillus subtilis CH1 by morphological, biochemical and 165 rRNA gene analysis. The optimal conditions for chitosanase production were investigated. The optimum carbon and nitrogen sources for Bacillus stibtilis CH1 were 2% starch and 1% yeast extract respectively. Unlike other chitosanases, the expression of this chitosanase was not induced or slightly induced with chitosan. The chitosanase secreted into the medium were concentrated with ammonium sulfate precipitation and purified by gel permeation chromatography. The molecular weight of purified chitosanase was 30 kDa. The optimum pH and temperature of purified chitosanase were 5.5 and $60^{\circ}C$ respectively. The purified chitosanase was continuously thermostable at $40^{\circ}C$ and showed stable activity between pH 6.0 and 8.0. Chitosanase activity of Bacillus subtilis CH1 under optimum condition was 4.1 units/ml.

• YAKHAK HOEJI
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• 제3권1호
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• pp.48-50
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• 1957

The micro-assay methods of vanillin have been shown quite few, though several methods have been introduced for determination. A new sensitive micro-determination of vanillin, applying the yellow color reaction of vanillin and anillin, is shown in this paper. The absorption maximum of the yellow coloring matter, 4-Oxy-3-methoxy-henzal aniline, was 435 mu. The one dimensional ascending paper chromatographic method is applyed for isolation of vanillin from the mixed sample. The microdetection of vanillin is studied only in this paper. Vanillin was detected by the yellow spot on paper trip by the coloring reagent of aniline after several hours paper strip chromatographic at the following condition; paper strip ................ 2.5 X 35cm Whatman Filter Paper No.2 developing Solvent ......... petroleum-benzene-methanol n-butanol-water coloring agent ............. aniline. The Rf-value on petroleum benzene and methanol was 0.63 and that on n-butanol and water was 0.90. The minimum detectable amount of vanillin by this method was 10 micrograms. It is recommendable, if interference substances as aromatic aldehydes present, that the application of this aniline reaction and Foline Denis reaction on the same paper chromatogram is appreciable.

• Journal of Environmental Health Sciences
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• 제32권4호
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• pp.381-385
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• 2006

For decolorization of synthetic dyes, One fungus(HUE05-1) which was isolated from textile wastewater collected from industrial complex in Korea showed excellent ability of removing synthetic dyes. This fungus was identified as Basidiomycetes species by Internal Transcribed Spacers (ITS) sequence. Isolated fungi. HUE05-1 completely decolorized all dyes in both solid and liquid condition. The decolorization results were Reactive Orange-16, 97.12%; Reactive Blue-19, 92.09%; Reactive Blue-49, 97.04%; Reactive Yellow-145, 95.53%; Acid Orange-10, 99.18%; Acid Violet-43, 98.73%; Acid Blue-350, 94.71% and Disperse Blue-106, 90.07%.

• Proceedings of the KIEE Conference
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• 대한전기학회 2001년도 춘계학술대회 논문집 전기기기 및 에너지변환시스템부문
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• pp.227-230
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• 2001

This paper deals with a bidirectional ac-dc converter used in ups system application. We propose a Voltage-Source-Charge-Pump-Power-Factor-Correction(VS-CPPFC) ac-dc converters. First of all, we propose a charge pump power-factor-correction converter. Secondly, we derive and analyse a unity power factor condition. The proposed topology is based on a half-bridge for the primary and a current-fed push pull for the secondary side of a high frequency isolation transformer. The advantage of bidirectional flow of power achieved by using the same power components is that the circuit is simple and efficient. And the galvanically isolated topology is specially attractive in battery charge/discharge circuits in ups system. We design equivalent model for the steady-state circuit and analyse operation waveforms for each mode. We show that the proposed model can be applied to ups system by simulation processes.

• Proceedings of the Microbiological Society of Korea Conference
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• 한국미생물학회 2000년도 추계학술발표대회
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• pp.85-91
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• 2000

Aspergillus oryzae is a filamentous fungus classified in the group Aspergillaceae Ascomycetes. It is an important microorganism for industrial production of enzymes and fermented food productions. It secrets large quantities of proteins or enzymes into the culture medium which makes this organism appealing for the production of heterologous proteins. Recently Electric field-mediated transformation method, electroporation, has been applied to fungal transformation. In this study, fungal transformation was carried out by bypassing the protoplast isolation step, decreasing the culturing time and non-protoplast transformation for the increment of transformation efficiency. Transformants were obtained with electroporation in optimal condition 2,500 voltage, 1,540 ohm and 0.50 capacitance. More than 1,000 transform ants were obtained with 6-10 hrs cultured mycelia without enzyme treatment, called non-protoplast transformation.

• The Korean Journal of Mycology
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• 제23권4호통권75호
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• pp.305-309
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• 1995

To investigate optimal conditions for the protoplast formation and regeneration of Phanerochaete chrysosporium, preparations of three enzymes were used to liberate protoplasts from its 20 hrs-old mycelium on cellophan membrane covered agar media. Novozym 234 alone with 0.6M sucrose was the most effective for isolation of protoplasts from the mycelium with 3hrs incubation time at $39^{\circ}C$ in shaking condition of 120 rpm. The poly-R medium stabilized with 0.6M mannitol was the best for regeneration of the protoplasts.

• Journal of Power Electronics
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• 제13권2호
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• pp.177-185
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• 2013

This paper studies a soft switching DC/DC converter to achieve zero voltage switching (ZVS) for all switches under a wide range of load condition and input voltage. Two three-level PWM circuits with the same power switches are adopted to reduce the voltage stress of MOSFETs at $V_{in}/2$ and achieve load current sharing. Thus, the current stress and power rating of power semiconductors at the secondary side are reduced. The series-connected transformers are adopted in each three-level circuit. Each transformer can be operated as an inductor to smooth the output current or a transformer to achieve the electric isolation and power transfer from the input side to the output side. Therefore, no output inductor is needed at the secondary side. Two center-tapped rectifiers connected in parallel are used at the secondary side to achieve load current sharing. Due to the resonant behavior by the resonant inductance and resonant capacitance at the transition interval, all switches are turned on at ZVS. Experiments based on a 1kW prototype are provided to verify the performance of proposed converter.

• Proceedings of the KSME Conference
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• 대한기계학회 2003년도 춘계학술대회
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• pp.717-722
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• 2003

To improve the shock resistance of the microdrive under non-operating conditions, an isolator was attached to the contacting part of the microdrive. Through FE analysis, design parameters for satisfying the allowable acceleration level of the spindle motor bearing part were presented, which is a most possible critical part of the microdrive.

• Korean Journal Plant Pathology
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• 제13권2호
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• pp.129-131
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• 1997

Occurrence of brown rot was observed on imported orange fruits, and irregular brown spots were appeared as an intial symptom. White colored mycelia were developed rapidly on the surface of the fruits under the humid condition and resulted in rotting of the fruits. The causal organism of brown rot of orange was identified as Phytophthora citrophthora. Most sporangia were nondeciduous, papillate, ovoid, and measured 25.5~51.5$\times$17.9~34.7 ${\mu}{\textrm}{m}$ in size. P. citrophtora was pathogenic on Satsuma mandarin as well as orange. Symptoms were produced 3~7 days after artificial inoculation of sporangia and mycelia mixture on the fruits of orange and Satsuma mandarin, which were similar to those on the naturally infected fruits.