• Proceedings of the Korean Society for Applied Microbiology Conference
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• 2001.06a
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• pp.149-153
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• 2001

To investigate the mode of bactericidal action for antimicrobial peptide, pediocin, synthetic and mutant pediocins were prepared by direct chemical synthesis. Native pediocin was purified from Pedio-coccus acidilactici M and its conformational structure and bactericidal functions were analyzed and compared to synthetic pediocin. Schematic mode of pediocin actions, how pediocin binds on the target cell membrane, penetrates and makes tunnel are proposed. For these purposes, primary and secondary structures of pediocin was analyzed and disulfide bond assignment was also done. The pediocin purified from P. acidilactici M had high effective bactericidal ability against gram positive bacteria, especially Listeria monocytogenes and was very stable at extreme pHs and even at high temperatures such as autoclaving temperature (121$^{\circ}C$). Pediocin was consisted of 44 amino acids with four cysteines. Novel synthetic peptides were achieved by solid phase peptide synthesis(SPPS) method. To explain the function of cysteine in C-terminal region, mutant pediocin, Ped[C24A＋C44A], was synthesized and their structural and biological functions were analyzed. Second mutant pediocin, Ped[KllE], was prepared to explain the function of lysine at 11 of N-terminal part of pediocin, especially loop of $\beta$-sheet, and to predict the initial binding site of pediocin. The native and synthetic pediocins was showed random coil conformation by spectropolarimetry in moderate conditions. This conformation was observed in extreme conditions such as high temperature and low and high pHs, also. Circular dichroism(CD) data also showed the existence of $\beta$-turn structure in N-terminal part both native and synthetic pediocins. A structural model for pediocin predicts that 18 amino acids in the N-terminal part of the peptide assume a three-strand $\beta$-sheet conformation. This random coil in C-terminal part of pediocin was converted to folding structure, helix structure, in nonpolar solvents such as alcohol and TFE. The disulfide bond between $^{9}$ Cys and $^{14}$ Cys was concrete and inevitable, however, evidences of disulfide bond between $^{24}$ Cys and $^{44}$ Cys was not. Data of Ped[C24A＋C44A], pediocin mutant showed that $^{44}$ Cys was required during killing the target cells but not inevitable, since Ped[C24A＋C44A] still have bactericidal activity but much less than native pediocin. Another pediocin mutant, Ped[KllE], had still bactericidal activity, was controversial to propose that positive charge like as $^{11}$ Lys in loop or hinge in bacteriocin bound or helped to binding to microorganism with electrostatic interaction between cell membrane especially teichoic acid and positive amino acid nonspecifically. The conformation of pediocin among native, synthetic and mutant pediocins did not show big difference. The conformations between oxidized and reduced pediocin were almost similar regardless of native or synthetic.

• Journal of the Korea institute for structural maintenance and inspection
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• v.16 no.5
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• pp.148-160
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• 2012

This study proposed more reasonable prediction models on compressive strength and carbonation of concrete structure and developed a more effective tunnel safety diagnosis and maintenance method through field application of the proposed prediction models. For this study, the Seoul Metro's Line 1 through Line 4 were selected as target structures because they were built more than 30 years ago and have accumulated numerous diagnosis and maintenance data for about 15 years. As a result of the analysis of compressive strength and carbonation, we were able to draw prediction models with accuracy of more than 80% and confirmed the prediction model's reliability by comparing it with the existing models. We've also confirmed field suitability of the prediction models by applying field, the average error of an estimate on compressive strength and carbonation depth was about 20%, which showed an accuracy of more than 80%. We developed a more effective maintenance method using durability prediction Map before field inspection. With the durability prediction Map, diagnostic engineers and structure managers can easily detect the vulnerable points, which might have failed to reach the standard of designed strength or have a high probability of corrosion due to carbonation, therefore, it is expected to make it possible for them to diagnose and maintain tunnels more effectively and efficiently.

• Journal of the Korea institute for structural maintenance and inspection
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• v.15 no.4
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• pp.107-115
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• 2011

In this study, the vibration analysis of the underground box structures induced train movement is studied. In order to perform these analysis, dynamic data, which was measured when subway is in service, are gained by attaching accelerometers on the structure such as lower beam, lateral wall and upper slab. Also, accelerometers are attached on the lower beams and side walls of the gravel ballast and concrete ballast sections in order to compare vibration due to ballast materials. The vibration results of upper slabs and lower beams reveal that the vibration on the upper slabs is greater than the lower beams. Also, the results of the crack gauge on the upper slab show that crack width dose not change due to vibration, These means that the effect of the vibration on the structure is very limited. In order to evaluate the vibration of the structure, acceleration unit is converted to velocity unit comparing with the existing velocity data gained from the platforms.

• Journal of the Korea institute for structural maintenance and inspection
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• v.22 no.4
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• pp.10-18
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• 2018

The primary purposes of this study are to understand a fundamental aspect of current uniformity around a reinforcing bar (rebar) in cement mortar, and to develop an accurate monitoring method in a wet-dry cycling process with the alternative current (AC) impedance method. Three cement mortar specimens with two embedded rebars were prepared in the laboratory. As a main variable, the distance between two rebars was designed to be 10, 20 and 30 mm with the same thickness of 20 mm. To simulate the corrosion of rebars in concrete structures in a marine environment, three cement mortar specimens were exposed to 15 wet-drying cycles (24-hour-immersion in seawater and 48-hour-drying in a room temperature) in the laboratory. It was observed that the potential level shifted to a noble value during corrosion potential monitoring, which is attributed to acceleration of dissolved oxygen diffusion at the drying process. AC impedance was measured in a frequency range from 100 kHz to 1 mHz on a wet-drying process. A theoretical model was proposed to explain the interface condition between the rebars and cement mortar by using the equivalent circuit consisting of a solution resistance, a charge transfer resistance and a CPE (constant phase element). It was observed that the diffusion impedance appeared in a low frequency range as corrosion of rebars progresses. At the drying stage of the wet-drying cycles, the currents line for monitoring tended to be non-uniform at the interface of rebar/mortar, being phase shift, ${\theta}$, close to $-45^{\circ}$.

• Journal of the Computational Structural Engineering Institute of Korea
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• v.33 no.5
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• pp.339-349
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• 2020

Double-leaf blast-resistant doors consisting of steel box and slab are application-specific structures installed at the entrances of protective facilities. In these structural systems, certain spacing is provided between the door and wall. However, variation in the boundary condition and structural behavior due to this spacing are not properly considered in the explosion analysis and design. In this study, the structural response and failure behavior based on two variables such as the spacing and blast pressure were analyzed using the finite element method. The results revealed that the two variables affected the overall structural behavior such as the maximum and permanent deflections. The degree of contact due to collision between the door and wall and the impact force applied to the door varied according to the spacing. Hence, the shear-failure behavior of the concrete slab was affected by this impact force. Doors with spacing of less than 10 mm were vulnerable to shear failure, and the case of approximately 15-mm spacing was more reasonable for increasing the flexural performance. For further study, tests and numerical research on the structural behavior are needed by considering other variables such as specifications of the structural members and details of the slab shear design.

• Journal of the Korea institute for structural maintenance and inspection
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• v.16 no.4
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• pp.18-24
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• 2012

In this study, hybrid method using polyvinyl acetate (PVAc) which has a strong adhesion and flexibility in which acrylic copolymer chemical-reaction reacts with cement, and is eco-friendly, is to improve the watertightness. The hybrid method is applied applied primarily waterproof stuff comprising silicate system and secondary mortar mixed with PVAc on the concrete surface. And then, in order to evaluate the performance, the properties of bond strength and amount of water absorption were measured. Based on the above experiments, mock-up specimens for field application were fabricated, and then the properties were evaluated as laboratory experiments. As the results, specimens cast from hybrid method using PVAc showed the best results on watertightness and bond strength. And also, with respect to experiment of mock-up specimens, the properties were in agreement with laboratory results. Especially, it could know that PVAc has strengthening effect from the results of the compressive strength. Due to outstanding results of carbonation depth and resistance to chloride ion penetration, it may be applied in weak areas such as underground and marine structures.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.31 no.6A
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• pp.457-464
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• 2011

An experimental study was performed to evaluate the possibility of using stiff type PolyUrea(PU) on RC slab as a strengthening material. Stiff type PU(STPU) was sprayed on the bottom surface of the slab specimens, which were then attached with CFRP or GFRP sheets. Also the evaluation of the bond capacity, the single most influential parameter on strengthening of RC structures, was carried out the flexural capacity evaluation test results showed that the load carrying capacity of the PU specimen was greater and less than the unstrengthened and FRP sheet attached specimens, respectively. The STPU specimens showed a ductile flexural behavior in the plastic displacement range. With respect to bond capacity, the bond strength of all of the specimen exceeded the code required bond strength of 1.5 MPa. Also, the STPU sprayed specimen without using epoxy resin did not peel off when the tensile grip was applied for testing. The stability of the PU bond failure indicate a good bond strength of PU when applied to concrete.

• Journal of the Korean Recycled Construction Resources Institute
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• v.4 no.4
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• pp.379-387
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• 2016

In this study, a survey was conducted on the required measurement and waterproofing performance for residential building underground structure leakage prevention system. According to the results of the survey, it has been determined that leakage problem is considered to be quite severe, and many respondents have agreed. The legal guidelines and regulation systems do not reflect properly on the environmental requirements or conditions, resulting in continued leakage problem. In regards to this, a standardized waterproofing technique that can be used in underground areas of residential structures is required as an obligation and the development of high performance waterproofing method that allows for wet concrete surface adhesion and a guideline, design and maintenance method that allows the control of overall situational control of leakage is required.

• Journal of the Korean Geosynthetics Society
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• v.19 no.1
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• pp.83-91
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• 2020

In this study, the characteristics of support behavior according to the change of ground condition of the cast-in-place pile and the large Caisson foundation, which are increasingly used as foundations of large structures and bridges. the allowable bearing capacity calculated using the yield load analysis method was analyzed to calculate similar allowable bearing capacity for each method. In addition, the allowable bearing capacity calculated by the ultimate load analysis method was found to have a large difference in bearing capacity for each method. Through this point, it can be usefully used as an empirical formula for evaluating the settlement characteristics of piles in future design and construction. In addition, as a result of examining the ground force distribution during sedimentation of large caissons, the section of the weathered rock layer showed almost constant ground force distribution as ground forces decreased after yield occurred at the base corner. And in the bed rock layer section, the foundation's center was transformed into a ground force in the form of a convex downward due to an increase in the ground resistance of the central part. Using these results, the theory previously presented by Fang (1991) and Kőgler (1936) was proved.

• Journal of the Korean Geotechnical Society
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• v.15 no.3
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• pp.161-176
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• 1999

A powerful numerical method that can be used for modeling rock-structure interaction is the Discontinuous Deformation Analysis (D D A) method developed by Shi in 1988. In this method, rock masses are treated as systems of finite and deformable blocks. Large rock mass deformations and block movements are allowed. Although various extensions of the D D A method have been proposed in the literature, the method is not capable of modeling water-block interaction, sequential loading or unloading and rock reinforcement; three features that are needed when modeling surface or underground excavation in fractured rock. This paper presents three new extensions to the D D A method. The extensions consist of hydro-mechanical coupling between rock blocks and steady water flow in fractures, sequential loading or unloading, and rock reinforcement by rockbolts, shotcrete or concrete lining. Examples of application of the D D A method with the new extensions are presented. Simulations of the underground excavation of the \ulcornerUnju Tunnel\ulcorner in Korea were carried out to evaluate the influence of fracture flow, excavation sequence and reinforcement on the tunnel stability. The results of the present study indicate that fracture flow and improper selection of excavation sequence could have a destabilizing effect on the tunnel stability. On the other hand, reinforcement by rockbolts and shotcrete can stabilize the tunnel. It is found that, in general, the D D A program with the three new extensions can now be used as a practical tool in the design of underground structures. In particular, phases of construction (excavation, reinforcement) can now be simulated more realistically.