• Title/Summary/Keyword: Concentration variation

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Analysis of Sap Production and Composition of Acer okamotoanum from Artificial Stands in Different Tapping Time in Jin-ju Region in Korea (진주지역 인공식재 우산고로쇠의 수액 채취 시기별 출수량 및 성분 분석)

  • HUH, Jin-Sung;KIM, Jeong-Woon;YOON, Jun-Hyuck;LEE, Su-Yeon
    • Journal of the Korean Wood Science and Technology
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    • v.48 no.2
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    • pp.206-216
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    • 2020
  • This study was conducted to analyze the sap flow and composition by tapping date in Acer akamotoanum from an artificial forest in the City of Jinju. The sap was collected from five saplings (diameter at breast height10-20 cm) in two phases with an interval of a month. During phase 1 (January 10, 2019 - February 9, 2019) with daily mean temperature of 1.16±2.15℃, 60.59 liters of sap was collected over 32 days. Phase 2 (February 12, 2019 - February 22, 2019) with daily mean temperature of 2.55±1.30℃ yielded 13.38 liters of sap over 11 days, which was a reduction to 22% of the total sap flow in phase 1. The analysis of sugar composition in the collected sap from phase 1 and 2 showed that sucrose concentration was 2.5% at its highest during the earlier days of collection and decreased to 0.8% at the end. Glucose and fructose concentrations were measured near 0, and were lower than sucrose concentration. The most prominent inorganic elements in the collected sap were potassium (41.69 - 89.75 mg/kg), calcium (24.66 - 48.14 mg/kg) and magnesium (10.18 - 27.25 mg/kg). The contents of sucrose and inorganic components between the samples collected from phase 1 and 2 did not show significant differences. The sap flow amounts of A. okamotoanum from different tapping periods showed a notable variation, but there were no significant differences in the contents of sugar or inorganic elements.

A Study on the Characteristics of Water Pollution in Rural Areas (농촌유역(農村流域)에서의 수질오염(水質汚染) 특성(特性)에 관한 연구(硏究))

  • Kim, Han-Tea;Kwun, Soon-Kuk
    • Korean Journal of Environmental Agriculture
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    • v.12 no.2
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    • pp.129-143
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    • 1993
  • The objective of this study is to understand the status of the water pollution in rural areas and to furnish a basic material for the management of the water pollution in rural areas. For this purpose, the Bokha river basin, Ichon-Gun, Kyungki-Do considering as a typical agricultural area was selected as a representative experimental watershed. The characteristics of water pollution in streams of the Bokha river basin was revealed by investigating and analyzing data collected for the source of pollution, water qualities in reaches of the stream, the degree of contribution to the river contamination by pollution mass produced from each source, and the status of the self-purification at the main stream. The most important source of the water pollution in investigated watershed was livestock, and the next important one were in the order of population, land use, and industry. The water quality of the Bokha river was relatively favorable judging from the BOD and COD concentration, however since the concentration of T-N and T-P showed significantly large values, it was concluded that the river was seriously contaminated by the nutrient material. The main cause of the river contamination was proved due to livestock waste. For the T-N, both land use and livestock were much more contributied to the pollution than any other source, which characterized the typical water pollution of rural areas. Run-off ratios for the Bokha river tributaries to the main stream were changed according to the similar trend to the variation of discharges in the branch streams. For the value of the self-purification constant at the main stream, it showed smaller value in the downstream reach than the middle-stream and upstream reaches, where could possibly have smaller reoxidation action due to slower velocity and deeper water depth.

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Relationship of Radon-222 and Chemical Composition of Groundwater as a Precursor of Earthquake (지진 전조인자로서 지하수내 라돈 및 화학성분의 상관성 연구)

  • Jeong, Chan Ho;Park, Jun Sik;Lee, Yong Cheon;Lee, Yu Jin;Yang, Jae Ha;Kim, Young Suk;Ou, Song Min
    • The Journal of Engineering Geology
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    • v.28 no.2
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    • pp.313-324
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    • 2018
  • In order to study the earthquake precursor in the Korean peninsula, long-term variations of chemical composition, radon-222, and water level were measured at depths (-60 m, -100 m) in the groundwater monitoring wells of the Daejeon and the Cheongwon area. The pH and electrical conductivity of groundwater in the monitoring wells showed some relationship with the Pohang earthquake. The ${HCO_3}^-$ and $Cl^-$ concentration of groundwater in the Daejeon and $Mg^{2+}$, $Cl^-$ and ${NO_3}^-$ in the Cheongwon showed some relation with the Pohang earthquake. However, it is not distinct to find the relationship between their variation and earthquake. The radon-222 concentration in Daejeon was observed a significant increase from a minimum of 162 Bq/L prior to the earthquake to 573 Bq/L right after the earthquake, that indicating a strong correlation with earthquakes. In the case of groundwater levels, it can not find some correlation between earthquakes and continuous decreasing trend in the monitoring wells of Daejeon and Cheongwon area. However, water level of a national groundwater observation well within 10 kilometers of Pohang epicenter was recorded as an abrupt drop right before the earthquake. Conclusively, although the location of monitoring wells is more than 180 kilometers apart from the epicenter of the Pohang earthquake, the radon gas in groundwater can be considered as a reliable candidate among earthquake precursors. The pH, electrical conductivity, ${HCO_3}^-$ and $Cl^-$ among hydrochemicals showed some correlation with earthquake should be monitored during a longer term to recognize distinctly as a precursor of earthquake.

Relationship between The Age and Chemical components of Ginseng Root's Portion(Panax ginseng C.A.Meyer) (인삼(人蔘)의 부위별(部位別) 및 연근별(年根別) 성분함량(成分含量)에 관(關)한 연구(硏究))

  • Lee, C.H.;Nam, K.Y.;Choi, K.J.
    • Korean Journal of Food Science and Technology
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    • v.10 no.2
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    • pp.263-268
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    • 1978
  • To find out the possible variation in chemical composition among ginseng products, the amount of saponin, extract and other basic components in different age and portion of ginseng roots(Panax ginseng Meyer) were investigated and compared with. (1) Great difference in the amount of ash, crude protein, fiber, fat, total sugar and reducing sugar was observed among different portion of the root comparing with those of different age of the root. That of ash, crude fiber, saponin and extract produced was higher in epidermis, fiber roots and subterranean stems, while that of crude protein, total sugar, panaxadiol/panaxatriol was higher in central portion and branch of the root. (2) The amount of extract produced was affected by the solvent used. Higher amount was obtained when water was employed. It was decreased as the increase of the concentration of alcohol solvent. Futhermore, the composition and physical properties were greatly varied by the concentration of alcohol solvent. (3) The amount of total-N, $P_2O_5,\;K_2O$, and ash was higher in two to three years old roots, while those of crude $SiO_2,\;CaO,$ crude fiber, and total sugar was higher in order roots. No difference was found in amount of MgO, Fe, Zn, and Na among age of the root.

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STUDIES ON THE EXTRACTION OF SEA WEED PROTEINS 2. Extraction of NaCl and Alcohol Soluble Proteins (해조단백질 추출에 관한 연구 2. 식염가용성 및 알콜가용성 단백질의 추출)

  • LEE Kang-Ho;RYU Hong-Soo;WOO Soon-Im
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.10 no.4
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    • pp.189-197
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    • 1977
  • In present study, the effects of various factors including the solvent concentration, extraction time and temperature, the ratio of sample vs extraction solvent, (w/v) and pH upon the extractability of the NaCl and alcohol soluble proteins of marine algae were investigated. Eight species of fresh algae, the major ones in consumption as food, namely Porphyra suborbiculata, Undarie pinnatifida, Hizikia fusiforme, Sargassum, fulvellum, Enteromorpha linza, Sargassum kjellmanianum, Codium coarctatum, and Ulva pertusa were used for the extraction of NaCl soluble protein and dried materials of four species, Perphyra suborbiculata, Undaria pinnatifida, Enteromorpha linza and Sargassum fulvellum were used for the extraction of alcohol soluble protein. The frozen and mascerated samples were prepared by the same method described in previous paper (Ryu, 1977). And the dried materials were moistened with alcohol solution before freezing. The effect of solvent concentration on the extractability of NaCl soluble protein differed from species. The extractability of Undaria Pinnatifide, Hizikia fusiforme, Perphyra suborbiculata, Enteromorpha linza, and Ulva pertusa reached maxima at 0.25M NaCl solution while the 1.0M for Sargassum fulvellum, Saygassum kjellmanianum and Codium coarctatum. In case of alcohol soluble proteins, it was shown at $20\%$ ethanol solution for Porphyra suborbiculata, Undaria pinnatifida, Enteromorpha linza, and Sargassum fulvellum. Variation of the ratio of sample vs solvent gave slight effect upon the extractability, but the ratio of 1:30(w/v) seemed most efficient for the extraction of NaCl soluble proteins and 100 ml solvent added to 1 g dried sample was effective in case of alcohol soluble proteins. Extraction time has a minimal effect upon the extraction of alcohol soluble protein, and approximately 21 to $43\%$ of algal protein was extracted within 1 hour. But in case of NaCl soluble protein extraction, the effect of time revealed differently from species to species resulting in that the extraction for 1 hour gave a maximum extractability in Ulva pertusa and Enteromorpha linza, 2 hours in Porphyra suborbiculata, Codium coarctatum and 3 hours in Undaria pinnatifica, Hizikia susiforme, Sargassum fulvellum and Sargassum kjellmanianum. When the NaCl soluble protein of Undaria pinnatifida and Enteromopha linza was extracted at various temperature, the most effective extraction temperature was $40^{\circ}C$ while the temperature was $50^{\circ}C$ for Undaria pinnatifida and $60^{\circ}C$ for Hixikia fusiforme, Sargassum fulvellum, Sargassum kjellmanianum and Codium coarctatum. Bus in case of alcohol soluble extraction, the optimum temperature was $30^{\circ}C$ for Enteromorpha linza and $40^{\circ}C$ for Undaria pinnatifida, Sargassum fulvellum and Porphyra suborbiculata. In the effect of pH on extractability, the maximum extractability of NaCl soluble proteins was obtained at pH 7to 8 and pH 8 to 9 for alcohol soluble protein.

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A Study on $Na^+$ and Water Reabsorption in the Nephron Segment Beyond Proximal Tubule Measured by Lithium Clearance

  • Han, O-Soo;Goo, Yong-Sook;Sung, Ho-Kyung
    • The Korean Journal of Physiology
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    • v.25 no.2
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    • pp.189-200
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    • 1991
  • During the past few years it has been proposed that lithium clearance can be used as a reliable measure for the outflow of tubular fluid from the proximal tubule. This study was aimed to characterize the inflow dependent reabsorption of Na in renal tubule beyond the proximal tubule. For this purpose, lithium clearance was used as a measure for the inflow from the proximal tubule and the changes in reabsorption fraction of Na and water were determined in rabbits. Rabbits were pretreated with hypotonic saline solutions for an hour (50 mM/L NaCl, 20 ml/hr/kg). And then a hypertonic solution of 500 mM/L NaCl (20 ml/kg) was administered intraperitoneally in conjunction with a bolus of LiCl solution (2 mM/kg, i.v.) for conditioning the $C_{Li}$ and urine flow rate. To rule out the effect of $Li^+$ on tubular functions, a bolus of NaCl solution (2 mM/kg, i.v.) was administered. Fifteen, thirty, and sixty minutes after injection of hypertonic saline arterial blood and urine samples were taken. Urinary and plasma concentrations as well as urinary output of $Li^+,\;Na^+\;and\;K^+$ were measured. From these $C_{Li},\;C_{Na}$ and the reabsorption fraction of Na and water $(Fr_{Na}\;&\;FrH_2O)$ were calculated. These results were compared with those from control groups in which the same amount of isotonic saline (145 mM/L NaCl) and of 15% dextran solution were administered in the same way as that in experimental group. Followings are the results obtained. 1) The plasma concentration of $Na^+$ in rabbits injected with hypertonic saline reached the peak value after 15 min and thereafter no significant change was observed. Hematocrit values did not show any change, while urinary excretion of $Na^+$ increased markedly during the first 15 min and decreased thereafter. These results were not affected by an injection of a small amount of LiCl. 2) The clearances of $Li^+,\;Na^+\;and\;K^+$ in rabbits injected with hypertonic saline and LiCl solution decreased. 3) In spite of the variation in $C_{Li},\;Fr_{Na}$ did not show any significant change while $FrH_2O$ increased gradually. 4) $C_{Li}$ decreased also in rabbits received isotonic saline. $Fr_{Na}$ tended to be higher than that in hypertonic saline group, while $FrH_2O\;and\;Fr_{Na}$ did not associated with the decrease in $C_{Li}$. 5) $C_{Li}$ of the rabbits received dextran solution fluctuated persistently and $Fr_{Na}\;and\;FrH_2O$ did not change in along with $C_{Li}$ although $Fr_{Na}$ had a tendency to be higher than that in hypertonic saline group. 6) From the above results it was concluded that: (a) In rabbits with normal body store of $Na^+$, the $Fr_{Na}$ of renal tubule beyond proximal tubule. calculated from $C_{Li}$ as a measure of inflow from proximal tubule is constant in spite of variations in $C_{Li}$. (b) The $FrH_2O$ calculated from $C_{Li}$ is dependent largely upon ADH rather than inflow from proximal tubule. (c) When there is a decrease in plasma $Na^+$ concentration or ineffective body fluid. $Li^+$ reabsorption may occur in the thick segnent of Henle's loop and hence the determination of $Fr_{Na}$ and $FrH_2O$ will not be easy one, but $Fr_{Na}$ is constant under the same experimental conditions.

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The characteristics of marine environment and phytoplankton community around southwestern waters for ichthyotoxic dinoflagellate Cochlodinium polykrikoides monitoring programme (남서해역의 유해성 적조생물 Cochlodinium polykrikoides Margalef 모니터링을 위한 환경특성 식물플랑크톤 군집 동태)

  • Cho Eun Seob;Choi Yong Kyu
    • Journal of Environmental Science International
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    • v.14 no.2
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    • pp.177-184
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    • 2005
  • This study was to determine the fluctuation in phytoplankton assemblages with regarding to environmental conditions and nutrients, which were surveyed around Mokpo waters in the southwestern waters, Korea. Sampling was carried out on the Mokpo, Sinan, and Wando coasts from March to November 2003. The maximum sea surface temperature was recorded in August, and it ranged around $25^{\circ}C$ regardless of sampling sites. However, salinity in Mokpo waters showed a great variation, which ranged from 5-30 psu and recoded the minimum of 5 psu in July and the maximum of 30 psu in November. Moreover, in Mokpo waters, the chlorophyll a and SS concentration of the surface layer were also the highest values of $20\;{\mu}g\;l^{-1}\;and\;40\;{\mu}g\;l^{-1}$, respectively than those of Sinan and Wando waters. The concentrations of $NH_4-N,\;NO_2-N,\;NO_3-N,\;and\;PO_4-P$ were also he highest values of $0.018\;{\mu}mol\;^l{-1},\;0.062\;{\mu}mol\;l^{-1},\;1.2\;{\mu}mol\;l^{-1}\;and\;0.078\;{\mu}mol\;l^{-1}$, respectively in Morpo waters than those of Sinan and Wando waters. During the period of this study, the majority of the taxa were diatoms; Thalassiosira rotula, Rhizosolenia setigera, Prorocentrum minimum, Chaetoceros curvisetus, Leptocylindrus danicus, Pseudonitzschia pungens, and Chaetoceros spp. were detected in the dominant species of phytoplankton. The dinoflagellates were relatively abundant during the summer season in Wando waters, which attained an abundance of $10-20\%$. In Mokpo waters, DIN/DIP was the highest value of 700 in March, whereas the lowest was shown in Wando waters. However, DIN/DIP value in summer at Wando waters was extremely reversed, which appeared to be associated with the development of dinoflagellates. On the bais of factor analysis using SYSAT 6.0, nutrient showed somewhat correlation with chlorophyll a. Consequently, the process of discharge of fresh water in Mokpo waters plays an important role in extremely fluctuation in nutrients and conditions. Although Wando waters maintains a lack of nutrients, it should be influenced by different water current and may be associated with a concentration of nutrients.

Intermediate Culture of the Scallop, Patinopecten yessoensis in the East Coast of Korea (동해안 참가리비, Patinopecten yessoensis의 중간육성)

  • Park, Young-Je;Rho, Sum;Lee, Jeong-Yong
    • Journal of Aquaculture
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    • v.13 no.4
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    • pp.339-351
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    • 2000
  • Optimal environmental conditions, that sustained fastest growth, lowest mortality and abnormality of the scallop Patinopecten yessoensis, were identified from field experiments undertaken at Chumunjin during 1991-1998. Temperature within the water column 10~30 m depth ranged between 5 and 23$^{\circ}C$; high temperature and daily fluctuation resulted in growth retardation and heavy mortality of the scallop. Optimal salinity range was between 31.5 and 34.5%0 and water transparency 6.0 and 18.1 m, which was significantly affected by phytoplankton density. Chlorophyll concentration ranged between 0.04 and 3.51 f.lgfL. Low temperature and high chlorophyll concentration appear to support faster growth of the scallop. Optimal periods of transplantation for intermediate culture were between mid July and early November: cultured under high density during July-August as a first step and under low density during mid September through early November as a second step. Optimal stocking density in square net cage (<35${\times}$35 em) for intermediate culture was 30-40 individuals per cage for main culture using lantern net and 80 -100 individuals of the size of 1.5 ~ 3.0 em shell height per cage for sowing culture. During the intermediate culture, the highest growth was realized, when the cage was held at water depth between 10 and 15 m. Water depth below 25 m, however, was best to avoid mass mortality during the periods of abnormally high water temperature and high variation of water temperature. The daily growth rate during the intermediate culture was between 0.019~0.381 mm; low in January and February but high in March and April. It is suggested that the main culture is commenced before June under low stocking density to avoid the possibility of mass mortality during summer by high water temperature.

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Effect of Nutrient Solution Concentration on the Growth and Mineral Uptake of Various Wrap-up Vegetables and Herbs Grown with Mixed Planting in DFT Hydroponics (담액 수경재배 시 양액농도 처리가 혼식한 쌈 채소류와 허브류의 생육과 무기양분 흡수에 미치는 영향)

  • Seo, Tae-Cheol;Rho, Mi-Young;Gang, Nam-Jun;Lee, Seong-Chan;Choi, Young-Hah;Yun, Hyung-Kweon
    • Journal of Bio-Environment Control
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    • v.16 no.4
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    • pp.395-406
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    • 2007
  • The twenty seven wrap-up vegetables (13 Compositae, 14 Brassicaceae) and seven herbs (6 Labiatae, 1 Umbelliferae) were cultivated with a deep flow technique (DFT) hydroponic beds and treated with 3 levels of nutrient solution concentrations of 1.2, 2.4, and $3.6dS{\cdot}m^{-1}$ in summer and autumn season. The pH and electrical conductivity (EC) change of nutrient solution, fresh weight, and mineral contents of plants were investigated. The pH was maintained lower in high electrical conductivity (EC) treatment and in summer than autumn. EC of nutrient solution in EC $3.6dS{\cdot}m^{-1}$ treatment increased up to $4.8dS{\cdot}m^{-1}$ during the growing period in summer season. The growth of tested plants showed high variations by plant species and nutrient solution concentrations. The coefficient variation (CV) of the shoot fresh weight of plants was higher in summer than autumn. The growth of Compostiae and herbs was better at EC $1.2dS{\cdot}m^{-1}$, and 14 Brassicaceae was better at EC $2.4dS{\cdot}m^{-1}$ in summer. In autumn, the growth was better at EC $2.4dS{\cdot}m^{-1}$ in all plants except kale 'TBC F1' and red rape 'honchaetae'. In mineral contents, total nitrogen and potassium were higher in autumn than summer. Total nitrogen, potassium, calcium, magnesium were higher in Brassicaceae than others. Iron and manganese, however, were higher in Compositae. As the results, this study suggests that mixed planting of 27 wrap-up vegetables and 7 herbs in DFT hydroponics in two seasons was possible and EC $1.2dS{\cdot}m^{-1}$ in summer and EC $2.4dS{\cdot}m^{-1}$ in autumn be recommended as for the nutrient solution concentration to produce them safely year round.

Time Course Variation of Liver 25-Bydroxyvitamin $D_3$ Content in Broiler Chicks Exposed to UVB Light with Different Dobe (상이한 선양의 자외선을 조사한 브로일러 병아리에 있어서 간장 25-Hydroxyvitamin $D_3$회량의 계치적 변화)

  • 장윤환;강훈석;여영수;김강수;조인호;배은경
    • Korean Journal of Poultry Science
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    • v.19 no.4
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    • pp.217-225
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    • 1992
  • This research was carried out to determine the 25-Hydroxyvitamin $D_3$[25(OH)$D_3$] content in liver of broiler Hubbard chicks fed vitamin VD-deficient diet for 31 days in a subdued light room and exposed to UVB light (maximum intensity at 297nm) with dose of 0.204 or 0.408 mJ/$\textrm{cm}^2$(30 or 60 min irradiation) . The lipid in liver collected at 0~138 hr after irradiation was extracted by chloroform-methanol(2:1, v /v) and 25(OH)$D_3$ fraction was separated by Sep-Pak silica cartridge. The 25(OH)$D_3$ concentration was measured by normal phase HPLC. The negative control chicks Presented 25(OH)D$_3$17.5 ng/g liver. When 0.204mJ/$\textrm{cm}^2$ was treated to whole body of chicks, the 25(OH)$D_3$ level was increased to 37.8 ng/g at 12 hr after irradiation, the peak concentration, 40.5 ng /g was appeared at the time of 86 hr, and decreasing trend was shown thereafter until 138 hr, the final time in this study. When 0.408 mJ/$\textrm{cm}^2$ was applied, the 25(OH)$D_3$ content was 36.7 ng /g liver at 12 hr, 61.4 ng/g(maximum value ) was appeared at 42 hr, and 39.5 ng /g at 138 hr. The increased absolute amounts in liver 25(OH)$D_3$ were 23 and 43.9 ng/g as chicks were exposed to UVB light with dose of 0.204 and 0.408mJ/$\textrm{cm}^2$, respectively. Consequently, it was found that when double dose of UVB light was irradiated to the chicks, their liver samples produced nearly double 25(OH)$D_3$ at 42 hr after exposure, and the peak value was presented earlier by 24 hr than that in the low dose treatment.

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