• Journal of Yeungnam Medical Science
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• 제35권2호
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• pp.213-218
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• 2018

Background: Body fats, especially both of abdominal fat pad mass and skeletal muscle fat content, are inversely related to insulin action. Therefore, methods for decreasing visceral fat mass and muscle triglyceride content may be helpful for the prevention of insulin resistance. Methods: Thalidomide, used for its anti-angiogenic and anti-inflammatory properties, was administered to rats for 4 weeks. A 10% solution of thalidomide in dimethyl sulfoxide was injected daily into the peritoneal cavity as much as 100 mg/kg of body weight. Results: The total visceral fat pad mass in the thalidomide-treated group was 11% lower than in the control group. The size of adipocytes of the epididymal fat pad mass in the thalidomide-treated group was smaller than in the control group. The intraperitoneal thalidomide treatment increased triglyceride concentrations by 16% in the red muscle, but not in the white muscle. Conclusion: The results suggested that intraperitoneal thalidomide treatment inhibited abdominal fat accumulation, and that the free fatty acids in the blood were preferentially accumulated in the red muscle rather than in the white muscle.

• 한국환경과학회지
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• 제20권2호
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• pp.175-183
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• 2011

This study was conducted to survey the subjective symptoms of workers and users in selected 5 public facility groups(offices, performance halls, private crammers, wedding halls and indoor sports facilities), located in Seoul, Daejeon and Chungnam Province. Overall as subjective symptoms that were worse at public facilities than those usual, following responses appeared: I have a headache, My eyes are dry or feel irritated or itching, I feel eyestrain or my eyes are bloodshot, My nose was stuffy, I have a cough, I feel dry in the throat or inflammation happens, I feel oppressed in the breast or sometimes have difficulty in breathing, My skin gets dry, My skin is itching or has some spots, I feel like vomiting, I feel easily tired or sleep, I lose my concentration and I feel my memory is falling, I feel dizzy, I feel depressed, I feel being sharp and feel tension, I feel muscular pain or stiffness on shoulders, back and neck. Also, we compare with normal subjective symptoms scores and subjective symptoms scores in public facilities. The highest subjective symptoms score in at normal was my skin gets dry(2.35 points) and subjective symptoms score in public facilities was I feel eyestrain or my eyes are bloodshot(2.61 points).

• 재활복지공학회논문지
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• 제8권4호
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• pp.275-281
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• 2014

본 연구에서는 가이드 폭과 위치조절을 통해 퍼팅 시 순차적인 집중력 강화를 유도하여 퍼팅능력향상을 이끌어낼 수 있는 퍼팅훈련시스템을 개발하고 유용성을 평가하였다. 시스템의 하드웨어는 LEGO NXT 콘트롤러와 금속 Tetrix 세트를 이용하여 개발하였으며 소프트웨어는 LabVIEW ver. 2010을 사용하였다. 시스템의 유용성 평가를 위해 비숙련자 10인과 숙련자(KPGA) 5인에 대해 개발된 시스템의 폭 위치 변화 퍼팅훈련을 10세트 실시하고 의견을 조사하였다. 실험결과 손목부위에서의 속도 가속도 변화 감소 및 머리부위의 변위 감소를 확인할 수 있었으며, 의견조사에서 집중력 향상과 동기부여에 도움이 된다는 소견을 얻을 수 있었다. 본 시스템의 가이드 폭 위치 조절방식의 훈련법은 퍼팅을 위한 것으로 비슷한 운동구조를 갖는 노약자 장애인용 파크골프나 게이트볼 훈련에도 쉽게 적용이 가능할 것으로 판단된다.

• 운동영양학회지
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• 제23권1호
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• pp.28-35
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• 2019

[Purpose] The purpose of this study was to investigate the effects of treadmill exercise on oxidative stress in the hippocampal tissue and mitochondrial dynamic-related proteins in rats fed a long-term high-fat diet (HFD). [Methods] Obesity was induced in experimental animals using high fat feed, and the experimental groups were divided into a normal diet-control (ND-CON; n=12), a high fat diet-control (HFD-CON; n=12) and a high fat diet-treadmill exercise (HFD-TE; n=12) group. The rats were subsequently subjected to treadmill exercise (progressively increasing load intensity) for 8 weeks (5 min at 8 m/min, then 5 min at 11 m/min, and finally 20 min at 14 m/min). We assessed weight, triglyceride (TG) concentration, total cholesterol (TC), area under the curve, homeostatic model assessment of insulin resistance, and AVF/body weight. Western blotting was used to examine expression of proteins related to oxidative stress and mitochondrial dynamics, and immunohistochemistry was performed to examine the immunoreactivity of gp91phox. [Results] Treadmill exercise effectively improved the oxidative stress in the hippocampal tissue, expression of mitochondrial dynamic-related proteins, and activation of NADPH oxidase (gp91phox) and induced weight, blood profile, and abdominal fat loss. [Conclusion] Twenty weeks of high fat diet induced obesity, which was shown to inhibit normal mitochondria fusion and fission functions in hippocampal tissues. However, treadmill exercise was shown to have positive effects on these pathophysiological phenomena. Therefore, treadmill exercise should be considered during prevention and treatment of obesity-induced metabolic diseases.

• Journal of Photoscience
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• 제12권2호
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• pp.87-93
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• 2005

A new assay technique to distinguish between pure compounds and the isomeric mixtures has been suggested using single molecule (SM) fluorescence detection technique. Since the number of emission spots in a fluorophorespread film prepared from a genuine dye solution was determined by experimental condition, the deviation of spot numbers from the expected values could be considered to be an indication of lower purity of the sample solution. The lower limit of sample concentration for this assay was determined to be $5{\times}10^{-10}$ M to show uniform number of expected spots within 10% uncertainties in our experimental condition. An individual fluorescence intensity distribution for a mixture of isomers having doubly different emissivities was simulated by adding distributions obtained from Cy3 and nile red (NR) independently. The result indicated that the mixture could be identified from the pure compounds through the difference in the number of Gaussian functions to fit the distribution. This new assay technique can be applied to the purity test for synthetic biofluorophores which are usually prepared in small quantities not enough for classical ensemble assays.

• 대한의생명과학회지
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• 제26권4호
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• pp.336-343
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• 2020

The present study was carried out to investigate the possibility that bispidinone derivative makes anticancer drug availability to human cervical carcinoma cell. The B8 has the lowest IC50 value among B8, B9 and B10 which are bispidinone analogue with bromide. According to cytotoxic test through WST-8 assay, B8 shows the most magnificent cytotoxicity effectiveness with 76 μM of IC50 value. In human cervical carcinoma cell treated with B8, it noticeably controlled cellular multiplication by increase of concentration and time. Furthermore, morphological changes like cellular shrink, disruption and nuclear condensation, feature of apoptosis, are observed. Annexin V-FITC/PI double staining assay test proved that B8 can cause apoptosis. Moreover, after treatment with 76 μM of B8, flow cytometry analysis shows that increase of active oxygen species are induced and membrane potential in mitochondria is decreased. Manifestation of Bcl-2 family and caspase cascades protein provides evidence that B8 induces apoptosis through mitochondria and caspase-related pathway. Taken together, we suggested that B8 reduced membrane potential in mitochondria and induce apoptosis through the pathway depended on mitochondria and caspase.

• 식품보건융합연구
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• 제7권3호
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• pp.25-32
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• 2021

This study examined the effects of kinesio taping on recovery from fatigue induced by an exercise of concentric contraction using an isokinetic machine. The fatigue was introduced by a mode of exercise at 60°/sec, 50 repetitions for three sessions. The changes of blood ammonia, lactate, LDH, and CK as a marker of fatigue were monitored. Eight healthy collegiate students participated in two experiments; 1) kinesio taping application condition and 2) no kinesio taping application condition. Before experiments, their physical characteristics were measured. For the experiment, blood samples were taken before and immediately after exercise, and 24hr as well as 72hr of recovery period. Window SPSS package 12.0 version was used and one-way ANOVA with repeated measures were employed. The results were as follows. 1) Blood ammonia tended to reduce along the recovery, but no differences between conditions were noticed. 2) Blood lactate tended to reduce during the recovery period, but differences between conditions were not noticed. 3) The LDH was not different between conditions and CK tended to reduce during the recovery period, but differences between conditions were not noticed. Based on the results, kinesio taping did not influence on recovery phase of blood ammonia, lactate concentration, LDH, and CK.

• 한국응용과학기술학회지
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• 제37권6호
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• pp.1646-1658
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• 2020

이 연구의 목적은 운동 후 수중회복방법의 차이가 성장호르몬, 테스토스테론, 혈중젖산농도, 심부담도, 통증의 변화를 알아보는 것이다. 저항운동 경력 6개월 이상인 남자 7명을 대상으로 집중 웨이트 트레이닝과 서킷 웨이트트레이닝을 1RM의 60%강도로 실시한 다음 회복방법으로 수중걷기회복과 수중앉기 회복을 진행하였다. 성장호르몬은 모든 운동형태와 회복방법에서 운동 후, 회복 후, 안정 시 순으로 높게 나타났으며, 테스토스테론은 운동 후, 회복 후, 안정 시 순으로 높았다. 혈중젖산농도는 모든 운동형태와 회복방법에서 운동 후, 회복 후, 안정 시 순으로 높았으며 집중저항운동 후 동적회복이 정적회복보다 낮았다. 심부담도는 모든 운동형태와 회복방법에서 운동 후, 회복 후, 안정 시 순으로 높았다. 근통증은 모든 운동형태와 회복방법에서 운동직후, 회복 후, 24시간, 48시간, 72시간 순으로 감소하였다. 물속 환경에서도 동적회복이 정적회복보다 근피로 개선에 효과가 있는 것으로 사료된다.

• Nutrition Research and Practice
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• 제12권3호
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• pp.199-207
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• 2018

BACKGROUND/OBJECTIVES: Fermented Laminaria japonica (FL), a type sea tangle used as a functional food ingredient, has been reported to possess cognitive improving properties that may aid in the treatment of common neurodegenerative disorders, such as dementia. MATERIALS/METHODS: We examined the effects of FL on scopolamine (Sco)- and ethanol (EtOH)-induced hippocampus-dependent memory impairment, using the Passive avoidance (PA) and Morris water maze (MWM) tests. To examine the underlying mechanisms associated with neuroprotective effects, we analyzed acetylcholine (ACh) and acetylcholinesterase (AChE) activity, brain tissue expression of muscarinic acetylcholine receptor (mAChR), cAMP response element binding protein (CREB) and extracellular signal-regulated kinases 1/2 (ERK1/2), and immunohistochemical analysis, in the hippocampus of mice, compared to current drug therapy intervention. Biochemical blood analysis was carried out to determine the effects of FL on alanine transaminase (ALT), aspartate transaminase (AST), and triglyceride (TG) and total cholesterol (TC) levels. 7 groups (n = 10) consisted of a control (CON), 3 Sco-induced dementia and 3 EtOH-induced dementia groups, with both dementia group types containing an untreated group (Sco and EtOH); a positive control, orally administered donepezil (Dpz) (4mg/kg) (Sco + Dpz and EtOH + Dpz); and an FL (50 mg/kg) treatment group (Sco + FL50 and EtOH + FL50), orally administered over the 4-week experimental period. RESULTS: FL50 significantly reduced EtOH-induced increase in AST and ALT levels. FL50 treatment reduced EtOH-impaired step-through latency time in the PA test, and Sco- and EtOH-induced dementia escape latency times in the MWM test. Moreover, anticholinergic effects of Sco and EtOH on the brain were reversed by FL50, through the attenuation of AChE activity and elevation of ACh concentration. FL50 elevated ERK1/2 protein expression and increased p-CREB (ser133) in hippocampus brain tissue, according to Western blot and immunohistochemistry analysis, respectively. CONCLUSION: Overall, these results suggest that FL may be considered an efficacious intervention for Sco- and EtOH-induced dementia, in terms of reversing cognitive impairment and neuroplastic dysfunction.

• Journal of Nutrition and Health
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• 제40권3호
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• pp.211-220
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• 2007

The purpose of this study was to investigate that the effect of dietary fatty acid composition on pro- and macro-glycogen utilization and resynthesis. The analyses were further extended for different muscle fibers (type I, type II, & type IIb) as well as tissues (i.e., liver & heart). Total one hundred sixty Sprague-Dawley rats were used, and rats were randomly allocated into four experimental groups: animals fed standard chow diet (n=40), animals fed saturated fatty acid diet (n=40), animals fed monounsaturated fatty acid (n=40), and animals fed polyunsaturated fatty acid (n=40). Animals in each groups were further divided into five subgroups: sacrificed at REST (n=8), sacrificed at immediately after 3 hr swim exercise (P-0HR, n=8), sacrificed at one hour after 3 hr swim exercise (P-1HR, n=8), sacrificed at four hour after 3 hr swim exercise (P-4HR, n=8), and sacrificed at twenty-four hour after 3 hr swim exercise (P-24HR, n=8). Soleus (type I), red gastrocnemius (type IIa), white gastrocnemius (type IIb), liver, and heart were dissected out at appropriated time point from all animals, and were used for analyses of pro- & macro-glycogen concentrations. After 8 weeks of dietary interventions, there was no significant difference in body mass in any of dietary conditions (p>.05). After 3 hr swim exercise, blood lactate level was higher compared to resting conditions in all groups, but it was returned to resting value after 1 hr rest (p<.05). Free fatty acid concentration was higher in all high fat fed groups(regardless of fatty acid composition) than CHOW consumed group. At rest, pro- & macro-glycogen concentration was not different from any of experimental groups (p>.05). Regardless of forms of glycogen, the highest level was observed in liver (p<.01), and most cases of supercompensation after 3hr exercise observed in this study were occurred in CHOW fed tissues. Except heart muscle, all tissues used in this study showed that pro- and macro-glycogen concentration was significantly decreased after 3 hr exercise. Based on these results, two conclusions were made: first, there is no different level of glycogen content in various tissues regardless of types of fatty acids consumed and second, the highest mobilization rate would be demonstrated from CHOW fed animals compare to animals that consumed any kinds of fatty acid diet if prolonged exercise is applied.