• The Journal of Korean Obstetrics and Gynecology
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• v.19 no.1
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• pp.14-30
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• 2006

Purpose : This study was conducted to investigate the inhibitory effects of Cortex ulmi pumilae on cell proliferation in HeLa cell. Methods : Human uterine cervical carcinoma HeLa cells were cultured in the 1%, 5% and 10% concentration of Cortex ulmi pumilae solution for 24 hours, 48 hours and 72 hours for the direct inhibitory effects of Cortex ulmi pumilae. Afterwards, we executed the analysis of the effect of Cortex ulmi pumilae solution on cell proliferation inhibition using XTT assay, DNA fragmentation, molecular biological method through MAP kinase activity and FACS analysis of caspase activity in the HeLa cells. Results : After 48 and 72 hours cultivation, the HeLa cells showed the concentration-dependently significant increase in all Cortex ulmi pumilae solution containing groups compared to the control. In the FACS analysis, all Cortex ulmi pumilae solution containing groups showed concentration-dependent increase compared to the control after 24 hours cultivation and the caspase-3 activities were decreased in all Cortex ulmi pumilae solution containing groups compared to the control after 24, 48 and 72 hours cultivation. After 48 and 72 hours cultivation, we could examined the apparent DNA fragmentation in all Cortex ulmi pumilae solution containing groups. In the XTT study, all Cortex ulmi pumilae solution containing groups showed concentration-dependent decrease compared to the control after 24 and 72 hours cultivation but 10% group after 48 hours and 5% and 10% groups after 72hours were presumed statistically significant differences. The expressions of MAP kinase were decreased in all Cortex ulmi pumilae solution containing groups compared to the control after 24, 48 and 72 hours cultivation. Conclusion : From this study we could suggest that Cortex ulmi pumilae be available to the inhibition of apoptosis of human cervical carcinoma cell line in vitro.

• Journal of Korean Neurosurgical Society
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• v.49 no.1
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• pp.13-19
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• 2011

Objective: The purpose of this study is to investigate the combined effects of ginkgo biloba extract, ginkgolide A and B and aspirin on SK-N-MC, human neuroblastoma cell viability and mRNA expression of growth associated protein43 (GAP43), Microtubule-associated protein 2 (MAP2), B-cell lymphoma2 (Bcl2) and protein53 (p53) gene in hypoxia and reperfusion condition. Methods: SK-N-MC cells were cultured with Dulbecco's Modified Eagle's Medium (DMEM) media in $37^{\circ}C$, 5% $CO_2$ incubator. The cells were cultured for 8 hours in non-glucose media and hypoxic condition and for 12 hours in normal media and $O_2$ concentration. Cell survival rate was measured with Cell Counting Kit-8 (CCK-8) reagent assay. Reverse transcriptase polymerase chain reaction (RT-PCR) was used to estimate mRNA levels of GAP43, MAP2, Bcl2, and p53 genes. Results: The ginkgolide A and B increased viable cell number decreased in hypoxic and reperfused condition. The co-treatment of ginkgolide B with aspirin also increased the number of viable cells, however, there was no additive effect. Although there was no increase of mRNA expression of GAP43, MAP2, and Bcl2 in SK-N-MC cells with individual treatment of ginkgolide A, B or aspirin in hypoxic and reperfused condition, the co-treatment of ginkgolide A or B with aspirin significantly increased GAP43 and Bcl2 mRNA levels. In MAP2, only the co-treatment of ginkgolide A and aspirin showed increasing effect. The mRNA expression of p53 had no change in all treating conditions. Conclusion: This study suggests that the combined treatments of Ginkgo biloba extracts and aspirin increase the regeneration of neuroblastoma cells injured by hypoxia and reperfusion.

• Journal of Korean Society for Geospatial Information Science
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• v.3 no.2 s.6
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• pp.29-42
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• 1995

Route design in topographical plane map brings many problems in efficiency and requires much time and labor by hand Recently, the active studies of efficient route design method using 3-D terrain information are being developed according to increasing concentration on GSIS. In order to analyze terrain information for route design efficiently, this study presents objective and overall datum by applying SQL in construction and analysis of database and the possibility of three-dimensional terrain information analysis, This study generates 3-D base map on topographical map of scale 1:5,000 and acquires terrain information that have various thematic map data; contour, land use, roadway, and drange. This is a study on the application of SQL in route design and construction of the terrain information that linked by graphic datum of completed topographical map and attributed datum of database. As the result of this study, we can produce promptly and efficiently design datum of profile annotation, cross section, and volume computations to the preliminary route for route design and apply this efficient method to route design by understanding visual DTM which is composed of the roadway and the natural scene after design.

• Journal of Environmental Health Sciences
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• v.49 no.1
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• pp.30-36
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• 2023

Background: Among various pollutants, fine particle (PM_2.5, defined as particle less than 2.5 nm in aerodynamic diameter) shows the most consistent association with adverse health effects. There is scientific evidence documenting a variety of adverse health outcomes due to exposure to PM_2.5. Objectives: This study aims to assess the health benefits of that would be achieved by meeting the World Health Organization's air quality guidelines for PM_2.5 using AirQ+ and BenMAP. Methods: We estimated PM_2.5 related health benefits in Korea from implementing the World Health Organization's air quality guidelines (annual average 5 ㎍/m³ and 10 ㎍/m³) and Korea's National Ambient Air Quality Standard (annual average 15 ㎍/m³). We used World Health Organization's AirQ+ and U.S. Environmental Protection Agency's Environmental Benefits Mapping and Analysis Program. Results: The annual number of avoided PM_2.5 related premature deaths exceeding WHO guideline levels was assessed using both AirQ+ and BenMAP. We estimated that the health benefits of attaining the World Health Organization's air quality guidelines for PM_2.5 (annual average 5 ㎍/m³) would suggest an annual reduction of 26,128 (95% confidence interval [CI]: 17,363~34,024) and 26,853 (95% CI: 18,527~34,944) premature deaths. Conclusions: Our study provided useful information to policy makers and confirms that the reduction of PM_2.5 concentration would result in significant health benefits in Korea.

• Proceedings of the Korean Geotechical Society Conference
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• 2003.03a
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• pp.509-512
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• 2003

Our country is serious difference of precipitation seasonally and about 66% of yearly mean rainfall is happening in concentration rainfall form between September on June. It requires consideration because of a lot of natural disasters by this downpour are produced. Slope failure is happened by artificial factor of creation of slope according to the land development, fill slope etc. and natural factor of rainfall, topography, nature of soil, soil quality, rock floor. Usually, Direct factor of failure slope is downpour. In this study, the Slope about among 55 places happened failure by downpour investigated occurrence position, geological etc and executed and inquire into character of rainfall connected with failure slope. Among character of rainfall, executed analysis about Max. hourly rainfall and cumulative rainfall of place that failure slope is situated and grasped the geological character of failure slope. Through this, inquire to character of failure slope by rainfall and take advantage of basis study for Hazard map creation.

• Journal of Environmental Impact Assessment
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• v.17 no.4
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• pp.213-224
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• 2008

Since 1970's rapid urbanization and economic growth, the population was highly increased and residential type was changed from single detached house to high density and high-rise apartment. Recently more high-rise residential building generates more traffic volumes and it makes spatial concentration. The highly traffic volumed road and high residential building was constructed nearby, it occurs noise problem. The purpose of this study is comparing the performance of three type noise barrier between noise source and receiver using a noise map simulation. Three types were composed as road side planting trees, building side planting trees and noise barrier wall. Planting trees is less effective than noise barrier wall as a noise barrier. And they are all effective under 3 stories than over 5 stories, especially noise barrier function can't work over 5 stories.

• Proceedings of the KSR Conference
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• 2007.11a
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• pp.1796-1816
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• 2007

Recently, started to apply a strategy of selection and concentration in the construction and transportation R&D for the effective utilization of limited R&D resources. The department of construction and transportation planned the construction and transportation R&D innovative road map by the future 10 years technical requirement under the first year on the innovation of the construction and transportation technology from last year. The innovative road map which is a strategic and long term master plan integrated a construction and transportation suggested a vision as value createar for a improvement of living quality in the future. And made a specific goal that is the seventh construction technology level and the fifth transportation technology level in the world. The department of construction and transportation is planning a mid- long range planing of the construction and transportation R&D as following measurement of innovative road map. KICTEP and KISTEP planned a mid- long range R&D planing of the transportation system, logistics, aviation, railway for the last year. Introducing a methodology used a mid- long range plan and main results of the future railway R&D plan. And suggesting a politic comments for the effectively propelling railway R&D projects.

• Journal of Korea Game Society
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• v.7 no.1
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• pp.3-10
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• 2007

The maps in the world of the role playing games provide not only areas for motions and actions of player characters, but also worlds of totally included monsters and NPC(Non Player Character). However, the design of maps and monsters in them could not be tested for their balances until the games had been developed quite a bit. So they need additional expense of extension period of developments. In this paper, we have designed and developed a map simulator to expect the popularities of the maps in the early stage of the game planning in order to prevent inefficient concentration of users on some maps by expecting the popularities of the maps in the early stage of game planning and by setting the parameters of monster arrangements in the map.

• New & Renewable Energy
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• v.10 no.2
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• pp.29-39
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• 2014

This study examines the future variability of surface wind speed and solar radiation based on climate change scenario over the Korean Peninsula. Climate change scenarios used in this study are RCP 4.5 and 8.5 with a 12.5 km horizontal resolution. Climate change scenario RCP 4.5 and 8.5 reproduce the general features of wind speed over the Korean Peninsula, such as strong wind speed during spring and winter and weak wind speed during summer. When compared with the values of wind speed and solar radiation of the future, they are expected to decrease current wind and solar resource map. Comparing the resource maps using RCP 4.5 and 8.5 scenarios, wind speed and solar radiation decrease with increasing greenhouse gas concentration. Meteorological resource maps of future wind and solar radiation should be improved with high resolution for the industrial application.

• Preventive Nutrition and Food Science
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• v.12 no.4
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• pp.222-228
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• 2007

This study was conducted to investigate the minimization of off-flavor occurrence and the maintenance of high quality in modified atmosphere packaged Pleurotus ostreatus during the storage. There are 4 treatments used to preserve high quality and for deodorization of MAP mushroom: Artemisia princeps, Artemisia capillaries, green tea and activated charcoal. The mushrooms were packed in polyethylene film with each treatment and were stored at 5 and $20^{circ}C$. No difference was observed in weight loss, $CO_2\;and\;O_2$ concentration, or color of mushrooms packed with or without treatment. However, the principal component analysis (PCA), electronic nose, revealed differences in off-flavor occurrence between control (MAP mushroom without treatment) and treatment groups at $5^{\circ}C$. This result suggested that Artemisia princeps and Artemisia capillaries was masking the off-flavor in MAP mushroom because the unique flavor of them was strongly revealed and green tea and activated charcoal might have a role of removing the off-flavor by adsorbing ethanol and acetaldehyde, which is known to cause off-flavor. The sensory test showed that Artemisia princeps and Artemisia capillaries dough treatment inhibited microbial growth.