• Proceedings of the Plant Resources Society of Korea Conference
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• 2010.10a
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• pp.14-14
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• 2010

Vitamin C (ascorbic acid) is an essential component for collagen biosynthesis and also for the proper functioning of the cardiovascular system in humans. Unlike most of the animals, humans lack the ability to synthesize ascorbic acid on their own due to a mutation in the gene encoding the last enzyme of ascorbate biosynthesis. As a result, vitamin C must be obtained from dietary sources like plants. In this study, we have developed two different kinds of transgenic potato plants (Solanumtuberosum L. cv. Taedong Valley) overexpressing strawberry GalUR and mouse GLoase gene under the control of CaMV 35S promoter with increased ascorbic acid levels. Integration of the these genes in the plant genome was confirmed by PCR and Southern blotting. Ascorbic acid(AsA) levels in transgenic tubers were determined by high-performance liquid chromatography(HPLC). The over-expression of these genes resulted in 2-4 folds increase in AsA intransgenic potato and the levels of AsA were positively correlated with increased geneactivity. The transgenic lines with enhanced vitamin C content showed enhanced tolerance to abiotic stresses induced by methyl viologen(MV), NaCl or mannitol as compared to untransformed control plants. The leaf disc senescence assay showed better tolerance in transgenic lines by retaining higher chlorophyll as compared to the untransformed control plants. Present study demonstrated that the over-expression of these gene enhanced the level of AsA in potato tubers and these transgenics performed better under different abiotic stresses as compared to untransformed control. We have also investigated the mechanism of the abiotic stress tolerance upon enhancing the level of the ascorbate in transgenic potato. The transgenic potato plants overexpressing GalUR gene with enhanced accumulation of ascorbate were investigated to analyze the antioxidants activity of enzymes involved in the ascorbate-glutathione cycle and their tolerance mechanism against different abiotic stresses under invitro conditions. Transformed potato tubers subjected to various abiotic stresses induced by methyl viologen, sodium chloride and zinc chloride showed significant increase in the activities of superoxide dismutase(SOD, EC 1.15.1.1), catalase, enzymes of ascorbate-glutathione cycle enzymes such as ascorbate peroxidase(APX, EC 1.11.1.11), dehydroascorbate reductase(DHAR, EC 1.8.5.1), and glutathione reductase(GR, EC 1.8.1.7) as well as the levels of ascorbate, GSH and proline when compared to the untransformed tubers. The increased enzyme activities correlated with their mRNA transcript accumulation in the stressed transgenic tubers. Pronounced differences in redox status were also observed in stressed transgenic potato tubers that showed more tolerance to abiotic stresses when compared to untransformed tubers. From the present study, it is evident that improved to lerance against abiotic stresses in transgenic tubers is due to the increased activity of enzymes involved in the antioxidant system together with enhanced ascorbate accumulated in transformed tubers when compared to untransformed tubers. At moment we also investigating the role of enhanced reduced glutathione level for the maintenance of the methylglyoxal level as it is evident that methylglyoxal is a potent cytotoxic compound produced under the abiotic stress and the maintenance of the methylglyoxal level is important to survive the plant under stress conditions.

• Journal of Korea Technology Innovation Society
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• v.10 no.4
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• pp.605-628
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• 2007

We analyze the embedded structure of the cooperative research network of innovation studies in Korea by employing the social network methodology. The network covers all the contributing authors of two journals such as JTI and KTIS, which are the representative journals in the area. Across the networks, the following is noted: networks are highly segmented and the link density is low. However, the KTIS network contains a huge component with 131 authors which implies the existence of a strong cooperative research infrastructure. We derived three sub-networks such as technology economics, technology management, and technology policy. The technology policy network has the lowest link density, while the technology management network of KTIS shows relatively high cohesion among the researchers. Simulation analysis for the mean links of networks by author affiliation divided into university and others show no significant difference between the two networks. We also found a higher ratio of linkage within affiliated network than between networks, rejecting our hypothesis that researchers will not discriminate their research partners due to strong governmental inducement of academia-res each-industry cooperation. The explainability of indices from network structure such as structural holes and clustering coefficients on the performance of researchers measured by the number of citations confirms the results reported in Oh, Choi & Kim (2006) which dealt with the research network of information systems.

• Journal of Convergence for Information Technology
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• v.10 no.11
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• pp.340-350
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• 2020

This study was conducted to present comprehensive professionalism measurement tools suitable for hospital administration staffs. The study used measurement tools related to the psychological and behavioral characteristics of individuals used in prior studies for administration staffs at general hospitals and hospitals. Factor analysis and reliability analysis were conducted to verify the validity and internal relevance. As a result of the analysis, it was concluded that composed of 5 factors, and that the total reliability was 94.4%, which was very reasonable as a comprehensive professionalism measurement tool. Therefore, each component was defined as Confidence of Job performance, Salary Adequacy, Pride in Duties, Ability to achieve work goals, and Work Autonomy in order to provide a clear meaning. Professionalism from a modern perspective is a broader concept than traditional professionalism, including the subjective perceptions of job values and individuals' their abilities. Therefore, comprehensive professionalism measurement tools presented in this study will be very suitable and useful for hospital administration staffs.

• The Journal of the Institute of Internet, Broadcasting and Communication
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• v.17 no.2
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• pp.15-23
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• 2017

The person Re-identification is the most challenging part of computer vision due to the significant changes in human pose and background clutter with occlusions. The picture from non-overlapping cameras enhance the difficulty to distinguish some person from the other. To reach a better performance match, most methods use feature selection and distance metrics separately to get discriminative representations and proper distance to describe the similarity between person and kind of ignoring some significant features. This situation has encouraged us to consider a novel method to deal with this problem. In this paper, we proposed an enhanced recurrent neural network with three-tier hierarchical network for person re-identification. Specifically, the proposed recurrent neural network (RNN) model contain an iterative expectation maximum (EM) algorithm and three-tier Hierarchical network to jointly learn both the discriminative features and metrics distance. The iterative EM algorithm can fully use of the feature extraction ability of convolutional neural network (CNN) which is in series before the RNN. By unsupervised learning, the EM framework can change the labels of the patches and train larger datasets. Through the three-tier hierarchical network, the convolutional neural network, recurrent network and pooling layer can jointly be a feature extractor to better train the network. The experimental result shows that comparing with other researchers' approaches in this field, this method also can get a competitive accuracy. The influence of different component of this method will be analyzed and evaluated in the future research.

• Journal of the Economic Geographical Society of Korea
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• v.19 no.2
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• pp.189-208
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• 2016

This research aims to discuss the impact of on regional development as a place brand and glocal heritage. Based on understanding of the changes and influences of local heritages in the globalization era as a key component of place branding, this study explores how is used to develop a place branding strategy for Lijiang. The research methods are both a literature review and a field research related to Lijiang and its culture. Also, the resources from news, internet, and YouTube are used to analyze the impact of . The performance has been attracting tourists from both Chineses and foreigners and contributed to increase the economic profits of local tourism industry as one of the representative identities of Lijiang. Also, in the process of preservation and recreation of cultural heritages of Lijiang such as , the participation of local residents and on-going interactions between the residents and global tourists highly influence on a transition from place marketing to place branding. By applying local cultural heritages to place branding strategies, the regional values of Lijiang strengthen its place identity from a place of preserving a minority's heritage to that of flourishing cultural exchanges and hybridization from the world.

• Journal of Navigation and Port Research
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• v.27 no.2
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• pp.209-215
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• 2003

In this paper. We tested and fabricated the error amplifier for the 15 Watt linear power amplifier for the IMT-2000 baseband station. The error amplifier was comprised of subtractor for detecting intermodulation distortion, variable attenuator for control amplitude, variable phase shifter for control phase, low power amplifier and high power amplifier. This component was designed on the RO4350 substrate and integrated the aluminum case with active biasing circuit. For suppression of spurious, the through capacitance was used. The characteristics of error amplifier measured up to 45 dB gain, $\pm$0.66 dB gain flatness and -15 dB input return loss. Results of application to the 15 Watt feedforward Linear Power Amplifier, the error amplifier improved with 27 dB cancellation from 34 dBc to 61 dBc IM$_3$.

• Korean Chemical Engineering Research
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• v.50 no.3
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• pp.527-534
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• 2012

The adsorption dynamics of activated carbon (AC) and carbon molecular sieve (CMS) beds were studied to recover ethylene from FCC fuel gas. In this study, the FCC fuel gas used consisted of six-component mixture ($CH_4/C_2H_4/C_2H_6/C_3H_6/N_2/H_2$,32:15:14:2:12:25 vol.%). And the breakthrough experiments of adsorption and desorption were carried out. The breakthrough sequence in the AC bed was $H_2$ < $N_2$ < $CH_4$ < $C_2H_4$ < $C_2H_6$ while the sequence in the CMS bed was $H_2$ < $CH_4$ < $N_2$ < $C_2H_6$ < $C_2H_4$. The separation performance of the CMS bed during the adsorption step was lower than that of the AC bed. However, due to the characteristics of kinetic separation, the CMS bed could remove $CH_4/N_2$ as well asthe molecules that are larger than $C_2H_6$, which was not easy to be done by the AC bed. Since it was hard to regenerate the adsorption bed by simple depressurization, vacuum regeneration should be adopted. As a result, the pressure vacuum swing adsorption (PVSA) process, consisting of CMS pretreatment process and AC main process, was suggested to recover ethylene efficiently.

• Journal of the Korean Chemical Society
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• v.53 no.6
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• pp.717-726
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• 2009

Multivariate models were developed for the simultaneous spectrophotometric determination of copper (II), nickel (II) and zinc (II) in water with 1-(2-thiazolylazo)-2-naphthol as chromogenic reagent in the presence of Triton X-100. To overcome the drawback of spectral interferences, principal component regression (PCR) and partial least square (PLS) multivariate calibration approaches were applied. Performances were validated with several test sets, and their results were then compared. In general, no significant difference in analytical performance between PLS and PCR models. The root mean square error of prediction (RMSEP) using three components for $Cu^{2+}$, $Ni^{2+}$ and $Zn^{2+}$ were 0.018, 0.010, 0.011 ppm, respectively. Figures of merit such as sensitivity, analytical sensitivity, limit of detection (LOD) were also estimated. High reliability was achieved when the proposed procedure was applied to simultaneous determination of $Cu^{2+}$, $Ni^{2+}$ and $Zn^{2+}$ in synthetic mixture and tap water.

• Journal of the Computational Structural Engineering Institute of Korea
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• v.33 no.2
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• pp.129-136
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• 2020

In this study, we develop a sampling-based seismic probabilistic risk assessment (SPRA) quantification technique that can accurately consider a partially dependent condition of component seismic fragility information. Specifically, the SPRA quantification method is proposed by combining the advantages of two representative methodologies: EPRI seismic fragility and JAERI seismic fragility input-based quantification. The most important feature of the proposed method is that it performs a SPRA using a sampling technique by transforming the EPRI seismic fragility input into JAERI seismic fragility input. When the proposed sampling-based approach was applied to an example of simple system and to a SPRA problem of a nuclear power plant, it was observed that the proposed method yields approximately similar system seismic fragility and seismic risk results as those of the exact solution. Therefore, it is believed that the approach proposed in this study can be used as a useful tool for accurately assessing seismic risks, considering the partial seismic dependence among the components; the existing SPRA method cannot handle such partial dependencies.

• Proceedings of KOSOMES biannual meeting
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• 2007.05a
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• pp.203-210
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• 2007

This paper treats optimal route safety assessment system at seaway based on weather forecasting data through INMARSAT. Since early times, captain have been sailing to select the optimum route considering the weather, ship loading status condition and operational scheduling empirically. However, it is rare to find digitalized onboard route support system whereas weather facsimile or wave and swell chart are utilized for the officer, based on captain's experience. In this paper, optimal route safety assessment system which is composed of voyage efficiency and safety component is introduced. Optimum route minimized ETA(estimated time of arrival) and fuel consumption that shipping company. and captain are requiring to evaluate for efficient voyage considering speed loss and power increase based on wave added resistance of ship. In the view point of safety, seakeeping prediction is performed based on 3 dimensional panel method Basically, the weather forecast is assumed to be prepared previously in order to operate this system.