• Toxicological Research
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• 제22권2호
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• pp.95-102
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• 2006

Exposure to soluble nickel compound produces toxic effects on immune system, but the mechanism of action remains to be elucidated. Differential gene expression was studied to understand the potential molecular mechanism responsible for acute toxicity induced by nickel acetate in spleen cells. We exposed mouse spleen cells to nickel acetate with a nontoxic dose ($40{\mu}M$) and then extracted total RNA at 6 h and 12 h after exposure. The RNA was hybridized onto 10K mouse oligonucleotide microarrays, and data were analyzed using GeneSpring 7.1. Nickel had a modest effects on expression of many genes, in the range of 1.3-3 fold. The expression profile showed time-dependent changes in expression levels of differentially expressed genes, including some important genes related to cell cycle, apoptosis and DNA repair. In hierarchical cluster analysis of duplicate experiments, 111 genes were screened out. Out of these, 44 genes showing time- dependent up-regulation (>1.5 fold) and 38 genes showing down-regulation (>1.5 fold) at all time points were chosen for further analysis. The change in the expression of three genes (GPX1, GADD45B and FAIM) after nickel treatment was validated using RT-PCR. As a rule, a number of genes appear to be coordinately regulated between cell survival and cell death from nickel toxicity. In conclusion, changes in the gene profile in the spleen after nickel treatment are complex and genes with diverse functions are modulated. These findings will be contributed to the understanding of the complicated biological effects of nickel.

• Clinical and Experimental Pediatrics
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• 제56권9호
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• pp.369-376
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• 2013

A complex interplay between genetic and environmental factors partially contributes to the development of allergic diseases by affecting development during prenatal and early life. To explain the dramatic increase in the prevalence of allergic diseases, the hygiene hypothesis proposed that early exposure to infection prevented allergic diseases. The hygiene hypothesis has changed to the microbial hypothesis, in which exposure to microbes is closely linked to the development of the early immune system and allergic diseases. The intestinal flora may contribute to allergic disease through its substantial effect on mucosal immunity. Based on findings that exposure to microbial flora early in life can change the Th1/Th2 balance, thus favoring a Th1 cell response, probiotics may be beneficial in preventing allergic diseases. However, evidence from clinical and basic research to prove the efficacy of probiotics in preventing allergy is lacking. To date, studies have yielded inconsistent findings on the usefulness of probiotics in allergic diseases. It is difficult to demonstrate an exact effect of probiotics on asthma, allergic rhinitis, and food allergy because of study limitations, such as different first supplementation period, duration, different strains, short follow-up period, and host factors. However, many studies have demonstrated a significant clinical improvement in atopic dermatitis with the use of probiotics. An accurate understanding of the development of human immunity, intestinal barrier function, intestinal microbiota, and systemic immunity is required to comprehend the effects of probiotics on allergic diseases.

• Journal of Radiation Protection and Research
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• 제35권1호
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• pp.12-20
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• 2010

원전 일차계통은 복잡한 수질환경으로 방사화생성물과 부식생성물 등 다양한 방사성핵종이 생성되고 있다. 이 방사성 핵종 중에서 원전종사자 피폭방사선량평가와 방사성유출물관리 측면에서 중요한 핵종으로는 $^3H,\;^{14}C,\;^{58}Co,\;^{60}Co,\;^{137}Cs,\;^{131}I$를 들 수 있다. 본 논문은 원전 방사선작업종사자와 원전 주변주민의 피폭방사선량에 기여가 큰 방사성핵종에 대해 살펴보고, 이들 핵종에 의한 선량평가 과정을 소개하였다. 특히 국내 원전에서 발생하였던 $^{131}I$ 내부피폭사건과 일차계통 냉각수의 탈염수 오염사건 등을 포함한 원전의 운영과정에서 일어났던 종사자와 원전주변주민에 대한 피폭 방사선량 평가 사례를 제시하였다. 또한 최근 이슈로 떠오른 삼중수소와 $^{14}C$의 선량평가에 대한 잠재적인 현안 등도 간단히 기술하였다.

• Safety and Health at Work
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• 제3권2호
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• pp.92-100
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• 2012

Objectives: The French National Occupational Diseases Surveillance and Prevention Network (RNV3P) is a French network of occupational disease specialists, which collects, in standardised coded reports, all cases where a physician of any specialty, referred a patient to a university occupational disease centre, to establish the relation between the disease observed and occupational exposures, independently of statutory considerations related to compensation. The objective is to compare the relevance of disproportionality measures, widely used in pharmacovigilance, for the detection of potentially new disease ${\times}$ exposure associations in RNV3P database (by analogy with the detection of potentially new health event ${\times}$ drug associations in the spontaneous reporting databases from pharmacovigilance). Methods: 2001-2009 data from RNV3P are used (81,132 observations leading to 11,627 disease ${\times}$ exposure associations). The structure of RNV3P database is compared with the ones of pharmacovigilance databases. Seven disproportionality metrics are tested and their results, notably in terms of ranking the disease ${\times}$ exposure associations, are compared. Results: RNV3P and pharmacovigilance databases showed similar structure. Frequentist methods (proportional reporting ratio [PRR], reporting odds ratio [ROR]) and a Bayesian one (known as BCPNN for "Bayesian Confidence Propagation Neural Network") show a rather similar behaviour on our data, conversely to other methods (as Poisson). Finally the PRR method was chosen, because more complex methods did not show a greater value with the RNV3P data. Accordingly, a procedure for detecting signals with PRR method, automatic triage for exclusion of associations already known, and then investigating these signals is suggested. Conclusion: This procedure may be seen as a first step of hypothesis generation before launching epidemiological and/or experimental studies.

• 혜화의학회지
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• 제8권1호
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• pp.403-424
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• 1999

To evaluate the antitumor activity, antimetastatic and radioprotective effects of Kamisasammaekmundongtang(KSMT), studies were done experimentally. The results were obtained as follows: 1. In cytotoxicity against P388, A549 and B16-F10, KSMT was not showed satisfiable cytotoxicity as compared with control. 2. In Inhibitory effect on activity of DNA topoisomerase I, KSMT has strong inhibitory effect. 3. The inhibitory effect on adhesion of A549 to complex extracellular matrix was significantly increased at 0.5mg/ml, 1mg/ml of KSMT. 4. The T/C% was 122 in KSMT treated group in S-180 bearing ICR mice. 5. In antiangiogenetic effect on CAM assay, inhibitory rate was 33% in KSMT treated group. 6. In pulmonary colonization assay, a number of colonies in the lungs were decreased significantly in KSMT treated group as compared with control group. 7. By FACS analysis of splenic leukocyte after exposure to radiation by linear accelerator, T-helper cell, B cell and macrophage in KSMT treated group were significantly increased while splenocytes were decreased in control group. 8. In histological changes of jejunum of $Bald{\setminus}C$ mice after exposure to radiation by linear accelerator, exclusion and fusion of villi were decreased as compared with control group. But in duodenum and ileum, exclusion and fusion of villi were not decreased as compared with control group. 9. WBC, PLT were increased in KSMT treated group as compared with control group after exposure to radiation by linear accelerator, but the increasing effect was not significant. Above results suggest that KSMT may be useful in prevention of cancer metastasis and protection from damage by radiotherapy. But the further study of KSMT would be demanded.

• 대한소아치과학회지
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• 제32권3호
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• pp.543-549
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• 2005

상악 견치는 가장 긴 치근을 가지며 저작기능과 함께 하악의 측방운동을 유도하고 심미적으로도 치열궁의 구각부위에 위치하여 교합관계의 조화와 대칭을 유지하는 등의 중요한 기능을 수행한다. 상악 견치는 발육기간이 가장 길며 형성되어 맹출하기까지 복잡한 경로를 갖기 때문에 다른 치아보다 매복의 빈도가 높다. 이러한 매복치를 치료하기 위해 임상의는 관찰을 하거나 외과적 노출과 교정적 견인, 자가치아이식, 발치 등 다양한 치료방법을 고려할 수 있다. 외과적 노출과 교정치료는 견치를 교합선상에 배치할 수 있기 때문에 가장 이상적인 접근으로 생각할 수 있다. 본 증례 보고는 구개측으로 매복된 상악 견치를 외과적 노출과 교정적 치아이동을 통해 기능적, 심미적으로 양호한 결과를 얻었다.

• Environmental Analysis Health and Toxicology
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• 제27권
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• pp.8.1-8.10
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• 2012

Objectives: The goal was to model and quantify the atmospheric concentrations of volatile organic compounds (VOCs) as the result of the Hebei Spirit oil spill, and to predict whether the exposure levels were abnormally high or not. Methods: We developed a model for calculating the airborne concentration of VOCs that are produced in an oil spill accident. The model was applied to a practical situation, namely the Hebei Spirit oil spill. The accuracy of the model was verified by comparing the results with previous observation data. The concentrations were compared with the currently used air quality standards. Results: Evaporation was found to be 10- to 1,000-fold higher than the emissions produced from a surrounding industrial complex. The modeled concentrations for benzene failed to meet current labor environmental standards, and the concentration of benzene, toluene, orthometa- para-xylene were higher than the values specified by air quality standards and guideline values on the ocean. The concentrations of total VOCs were much higher than indoor environmental criteria for the entire Taean area for a few days. Conclusions: The extent of airborne exposure was clearly not the same as that for normal conditions.

• Toxicological Research
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• 제35권2호
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• pp.155-160
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• 2019

Zinc pyrithione (ZnPT) is a coordination complex of zinc and has been used widely as an anti-dandruff agent in shampoos. Many shampoos contain both ZnPT and EDTA, a chelating agent speculated to increase ZnPT absorption, thereby raising concerns about neurotoxicity. Here, we investigated the effect of EDTA on ZnPT absorption by direct comparison of ZnPT and pyrithione (PT) concentrations in shampoo formulations, and by pharmacokinetic analysis of ZnPT, PT, and 2-methanesulfonylpyridine (MSP), the main ZnPT metabolite, in rat plasma or urine following exposure to shampoo containing ZnPT alone or a combination of ZnPT and EDTA. Approximately 17.3% of ZnPT was converted to PT by the addition of EDTA in the shampoo formulation. Plasma ZnPT and PT concentrations were not measured up to 24 hr after treatment with shampoo containing 1% ZnPT or 1% ZnPT + 2% EDTA in all rats. However, PT amount in 24-hr urine sample, MSP concentration in plasma, and MSP amount in 24-hr urine sample were approximately 4-, 2.6-, and 2.7-fold higher, respectively, in the 1% ZnPT + 2% EDTA shampoo group than in the 1% ZnPT shampoo group. As confirmed by the formulation analysis and in vivo pharmacokinetic analysis, the exposure of ZnPT could be increased by the absorption of PT due to partial dissociation of ZnPT into PT.

• 대한지리학회지
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• 제38권3호
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• pp.389-399
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• 2003

우주 기원의 방사성 핵종(核種)은 근래에 지형연구에 널리 활용되고 있다. 본 연구에서는 우주 기원의 방사성 핵종인 $^{10}$ Be과 $^{26}$ Al를 이용하여 만어산의 토르와 암괴류를 대상으로 그 구성 암석의 지표 노출 연대와 침식률을 측정하였다. 연구 결과, 토르는 적어도 약 6만 5천년 전, 그리고 암괴류는 적어도 3만 8천년 전에 노출된 것으로 나타났다. 한편, 토르와 암괴류의 구성 암석의 표면 침식률은 각각 9m/Ma 15m/Ma인 것으로 나타났다. 이러한 침식률의 차이는 풍화 및 침식에 대한 국지적인 조건이 다르기 때문인 것으로 생각된다. 특이한 점은, 산정부에 위치한 토르의 구성 암석들 중 일부가 $^{26}$ Al/$^{10}$ Be과 $^{10}$ Be를 이용하는 Lal (1991)이 밝힌 정상상태 침식구역(steady-state erosion island)에서 벗어나 있는데 이것은 이 토르의 표면이 비정상적인 침식의 경로를 겪었다는 것을 의미하며 본 연구에서는5cm-60cm 정도의 표면이 여러 번 떨어져 나갔다는 것으로 판단된다.

• 한국환경보건학회지
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• 제46권2호
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• pp.136-149
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• 2020

Objectives: The purpose of this study was to perform an aggregate human risk assessment for benzene in an industrial complex using the CalTOX model and to improve the reliability and predictability of the model by analyzing the uncertainty and sensitivity of the predicted assessment results. Methods: The CalTOX^TM 4.0 beta model was used to evaluate a selected region, and @Risk 7.6 software was used to analyze uncertainty and sensitivity. Results: As a result of performing the aggregate risk assessment on the assumption that 6.45E+04 g/d of benzene would be emitted into the atmosphere over two decades, 3% of the daily source term to air remained in the selected region, and 97% (6.26E+04 g/d) moved out of the region. As for exposure by breathing, the predicted LADD_inhalation was 2.14E-04 mg/kg-d, and that was assessed as making a 99.99% contribution to the LADD_total. Regarding human Risk_cancer assessment, the predicted human cancer risk was 5.19E-06 (95% CI; 4.07E-06-6.81E-06) (in the 95th percentile corresponding to the highest exposure level, a confidence interval of 90%). As a result of analyzing sensitivity, 'source term to air' was identified as the most influential variable, followed by 'exposure time, active indoors (h/day)', and 'exposure duration (years)'. Conclusions: As for the results of the human cancer risk assessment for the selected region, the predicted human cancer risk was 5.19E-06 (95% CI; 4.07E-06-6.81E-06) (in the 95th percentile, corresponding to the highest exposure level, a confidence interval of 90%). As a result of analyzing sensitivity, 'source term to air' was found to be most influential.