• Journal of Marine Bioscience and Biotechnology
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• v.11 no.2
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• pp.52-61
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• 2019

Over the past few decades, microalgae-based biotechnology conjugated with innovative CRISPR/Cas9-mediated genetic engineering has been attracted much attention for the cost-effective and eco-friendly value-added compounds production. However, the discharge of reproducible living modified organism (LMO) into environmental condition potentially causes serious problem in aquatic environment, and thus it is essential to assess potential environmental risk for human health. Accordingly, in this study, we monitored discharged genetically modified microalgae (GMM) near the research complex which is located in Daejeon, South Korea. After testing samples obtained from 6 points of near streams, several green-colored microalgal colonies were detected under hygromicin-containing agar plate. By identification of selection marker genes, the GMM was not detected from all the samples. For the lab-scale environmental risk assessment of GMM, acute toxicity test using rotifer Brachionus calcyflorus was performed by feeding GMM. After feeding, there was no significant difference in mortality between WT and transformant Chlamydomonas reinhardtii. According to further analysis of horizontal transfer of green fluorescence protein (GFP)-coding gene after 24 h of incubation in synthetic freshwater, we concluded that the GFP-expressed gene not transferred into predator. However, further risk assessments and construction of standard methods including prolonged toxicity test are required for the accurate ecological risk assessment.

• Journal of Broadcast Engineering
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• v.21 no.3
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• pp.341-348
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• 2016

HEVC is the next compression standard and is expected to be used widely replacing the conventional H.264/AVC standard. The compression ratio of the HEVC is twice times than H.264/AVC, whereas its computational complexity is increased by up to 40%. Many research efforts have been made to reduce the computational complexity and to speed up encoding. For intra coding, the rough mode decision (RMD) is commonly applied. The rate-distortion optimization (RDO) process to decide the best mode is too complex so that RMD chooses the candidate modes with a simple process and sends the candidates to RDO process. However, for large-size blocks, the RMD also requires considerable computations. In this paper, a down-sampling scheme is proposed for the RMD process. The reference pixel loading, predicted pixel generation are performed using the down-sampled pixel data. When the proposed scheme is applied to the RMD, the computational complexity is reduced by 70% with a marginal bitrate increase of 0.04%. In terms of area of hardware-based RMD, the gate count and the buffer size is reduced 33% and 66%, respectively.

• Journal of Microbiology
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• v.42 no.3
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• pp.188-193
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• 2004

Xanthobacter flavus strain UE15 was isolated in wastewater obtained from the Ulsan industrial complex, Korea. This strain functions as a 1,2-dichloroethane (1,2-DCA) degrader, via a mechanism of hydrolytic dechlorination, under aerobic conditions. The UE15 strain was also capable of dechlorinating other chloroaliphatics such as 2-chloroacetic acid and 2-chloropropionic acid. The dhlA gene encoding 1,2-DCA dechlorinase was cloned from the genomic DNA of the UE15 strain, and its nucleotide sequence was determined to consist of 933 base pairs. The deduced amino acid sequence of the DhlA dechlorinase exhibited 100％ homology with the corresponding enzyme from X. autotrophicus GJ10, but only 27 to 29％ homology with the corresponding enzymes from Rhodococcus rhodochrous, Pseudomonas pavonaceae, and Mycobacterium sp. strain GP1, which all dechlorinate haloalkane compounds. The UE15 strain has an ORF1 (1,356 bp) downstream from the dhlA gene. The OFR1 shows 99％ amino acid sequence homology with the transposase reported from X. autotrophicus GJ10. The transposase gene was not found in the vicinity of the dhlA in the GJ10 strain, but rather beside the dhlB gene coding for haloacid dechlorinase. The dhlA and dhlB genes were confirmed to be located at separate chromosomal loci in the Xanthobacter flavus UE15 strain as well as in X. autotrophicus GJ10. The dhlA and transposase the UE15 strain were found to be parenthesized by a pair of insertion sequences, 181247, which were also found on both sides of the transposase gene in the GJ10 strain. This unique structure of the dhlA gene organization in X. flavus strain UE15 suggested that the dechlorinase gene, dhlA, is transferred with the help of the transposase gene.

• Proceedings of the KSAR Conference
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• 2001.03a
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• pp.47-47
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• 2001

The neuropeptide vasopressin (VP) is a nine- amino acid hormone synthesized as preprohormone in the cell bodies of hypothalamic magnocellular neurons. The tumor in magnocellular neurons of the hypothalamus is associated with disfunctions of the cell bodies, leading to the diabetes insipidus. In order to study with the diabetes insipidus caused by a defect in VP synthesis and its secretion, we have produced the transgenic mice regulated by vasopressin promoter inserted to SV40 T antigen coding sequence (pVPSV.IGR2.1). One transgenic line expressing high levels of SV40 T antigen was propagated. The founder and all transgene positive adult animals have appeared with shorten mortality or apparent phenotypic abnormalities, including immune complex disease, and eventually die between 4 and 8 months of age. The mRNA and protein of SV40T antigen transgene were detected in brain of fetus as well as in brain, spleen, lung and lymph node in moribund at the age of 20 weeks. Histological analysis of transgenic mice showed that tumor developed in brain similar to primitive neuroectodermal tumors (PNET) in man. We also detected lymphomas in spleen and lymph node, and consequent tumor formation in various tissues of the transgenic mice. In pVPSV.IGR2.1, 21% mice showed brain tumor (PNET) at 5 weeks and 100% mice showed brain tumor after 15 weeks. In addition, Expression of apoptosis related genes (Bcl-28 & Bax) was increased over their age in mice with PNET as compared to control mice. Apoptosis related gene expression might be deregulated in mice with brain tumor. However, transgenic mice were not developed with the diabetes insipidus. These mice represent the first disease model to exhibit primitive neuroectodermal tumor in brain, as well as a unique model system for exploring the cellular pathogenesis of lymphomas.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.32 no.5A
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• pp.503-509
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• 2007

Low-power motion estimation is required for video coding in portable information devices. In this paper, we propose a low-power motion estimation algorithm and 1-D systolic may VLSI architecture using full search block matching algorithm (FSBMA). Main power dissipation sources of FSBMA are complex computations and frequent memory accesses for data in the search area. In the proposed algorithm, memory accesses and computations are reduced by using 1D PE (processing array) array architecture performing motion estimation of two neighboring blocks in parallel and by skipping unnecessary computations during motion estimation. The VLSI implementation results of the algorithm show that the proposed VLSI architecture can save 9.3% power dissipation and can operate two times faster than an existing low-power motion estimator.

• KIPS Transactions on Software and Data Engineering
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• v.3 no.8
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• pp.299-308
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• 2014

Various literatures related computing of information processing have been recently shown the researches inspired from the remarkably excellent human capabilities which recognize and categorize very complex visual patterns such as body motions and facial expressions. Applied from human's outstanding ability of perception, the classification function of visual sequences without context information is specially crucial task for computer vision to understand both the coding and the retrieval of spatio-temporal patterns. This paper presents a biological process based action recognition model of computer vision, which is inspired from visual information processing of human brain for action recognition of visual sequences. Proposed model employs the structure of neural fields of bio-inspired visual perception on detecting motion sequences and discriminating visual patterns in human brain. Experimental results show that proposed recognition model takes not only into account several biological properties of visual information processing, but also is tolerant of time-warping. Furthermore, the model allows robust temporal evolution of classification compared to researches of action recognition. Presented model contributes to implement bio-inspired visual processing system such as intelligent robot agent, etc.

• Convergence Security Journal
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• v.12 no.4
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• pp.115-122
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• 2012

As the most common application development of software development time, error-free quality, adaptability to frequent maintenance, such as the need for large and complex software challenges have been raised. When developing web applications to respond to software reusability, reliability, scalability, simplicity, these quality issues do not take into account such aspects traditionally. In this situation, the traditional development methodology to solve the same quality because it has limited development of new methodologies is needed. Quality of applications the application logic, data, and architecture in the entire area as a separate methodology can achieve your goals if you do not respond. In this study secure coding, the big issue, web application factors to deal with security vulnerabilities, web application architecture, design procedure is proposed. This proposal is based on a series of ISO/IEC9000, a web application architecture design process.

• The Journal of the Institute of Internet, Broadcasting and Communication
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• v.17 no.4
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• pp.95-106
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• 2017

In this paper, we investigate the conventional topological interference management (TIM) with proposed network topology such as 8 trigram (8 user networks). The key observation is that optimal symmetric degree of freedom (DoF) can be achieved for 8 user network with different channel coherence times by adaptively selecting the interference alignment scheme via controlling the alignment feasibility of the transmitted signals. However, this yields a very complex problem, for which we use the combination of different schemes such as interference avoidance and repetition coding. In addition to the above schemes, we propose a triangular transmit cooperation (TTC) algorithm for 8 user networks to achieve the optimal symmetric DoF. And We apply the principle of complementarity of 8 trigram to remove the interferences, and correspond the concepts of win-win and conflict to direct and indirect signals of transmit and receive respectively. We find that the principle of complementarity comes out from the trigram of I Ching. That is, we apply the relation of confrontation and coexistence to 8 transmitters and receivers, and get the results of symmetric DoF of 4/3.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.39C no.8
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• pp.651-659
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• 2014

In this paper, we consider a two way relay network for ship-to-ship communications in a fleet, where two communicating ships exchange the information with the help of a multi-antenna relay ship. For the network, we propose a rate-aware three-phase analog network coding to improve the reliability of the information exchange with asymmetric rates. The proposed scheme allows low-complex implementation of the relay without channel estimation by generating an improved analog network coded signal with the orthogonally received signals from two ships by using only the received signal power at each antenna. In addition, the proposed scheme reduces outages in the data exchange at asymmetric rates by adopting a rate-aware relay power allocation, which is confirmed by evaluating the outage performance via simulation.

• Journal of Hydrogen and New Energy
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• v.22 no.4
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• pp.451-457
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• 2011

Purple non-sulfur (PNS) bacterium $Rhodobacter$ $sphaeroides$ KD131 was studied with the aim of achieving maximum hydrogen production using various carbon and nitrogen sources at different pH conditions. Cells grew well and produced hydrogen using $(NH_4){_2}SO_4$ or glutamate as a nitrogen source in combination with a carbon substrate, succinate or malate. During 48h of photo-heterotrophic fermentation under 110$W/m^2$ illumination using a halogen lamp at $30^{\circ}C$, 67% of 30mM succinate added was degraded and the hydrogen yield was estimated as 3.29mol $H^2$/mol-succinate. However, less than 30% of formate was consumed and hydrogen was not produced due to a lack of genes coding for the formate-hydrogen lyase complex of strain KD131. Initial cell concentrations of more than 0.6g dry cell weight/L-culture broth were not favorable for hydrogen evolution by cell aggregation, thus leading to substrate and light unavailability. In a modified Sistrom's medium containing 30mM succinate with a carbon to nitrogen ratio of 12.85 (w/w), glutamate produced 1.40-fold more hydrogen compared to ammonium sulfate during the first 48h. However, ammonium sulfate was 1.78-fold more effective for extended cultivation of 96h. An initial pH range from 6.0 to 9.0 influenced cell growth and hydrogen production, and maintenance of pH 7.5 during photofermentation led to the increased hydrogen yield.