• The Plant Pathology Journal
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• 제30권2호
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• pp.117-124
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• 2014

The plant pathogenic bacterial genus Pectobacteirum consists of heterogeneous strains. The P. carotovorum species is a complex strain showing divergent characteristics, and a new subspecies named P. carotovorum subsp. brasiliensis has been identified recently. In this paper, we re-identified the P. carotovorum subsp. brasiliensis isolates from those classified under the subspecies carotovorum and newly isolated P. carotovorum subsp. brasiliensis strains. All isolates were able to produce plant cell-wall degrading enzymes such as pectate lyase, polygalacturonase, cellulase and protease. We used genetic and biochemical methods to examine the diversity of P. carotovorum subsp. brasiliensis isolates, and found genetic diversity within the brasiliensis subsp. isolates in Korea. The restriction fragment length polymorphism analysis based on the recA gene revealed a unique pattern for the brasiliensis subspecies. The Korean brasiliensis subsp. isolates were divided into four clades based on pulsed-field gel electrophoresis. However, correlations between clades and isolated hosts or year could not be found, suggesting that diverse brasiliensis subsp. isolates existed.

• Journal of Plant Biotechnology
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• 제37권1호
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• pp.1-11
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• 2010

Floral scent emitted from many plants is the key factor for pollinator attraction and defense for survival in nature and is important industrial materials for perfumery as well. It is a complex mixture of various organic molecules with a high volatility or a high vapor pressure. In general, floral scents are divided into three categories, aliphatics, terpenoids, and phenylpropanoids/benzenoids, based on its origin. About 1,700 scent compounds have been identified and their biochemistry and molecular biology also have elucidated their biosynthesis from various flowering plants during the last ten years. In addition to improvement of vase life, flower color and shape, and/or disease resistance, floral scent is coming up to the major breeding target for improvement of marketability. Therefore, metabolic engineering can be an important tool in near future and may be able to facilitate the breeding program for novel cultivar selection and improvement of marketability of floricultural crops.

• Animal cells and systems
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• 제16권2호
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• pp.154-161
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• 2012

We reviewed the genus $Adoxophyes$ (Lepidoptera: Tortricidae) from Korea and recognized three species of the genus. Among them, $A.$ $paraorana$ sp. nov., formerly misidentified as $A.$ $orana$ in Korea, is described as new to science. $Adoxophyes$ $orana$, a notorious pest known in most Eurasian countries for a long time, turns out to be only recently introduced or found in Korea. Photographs of the adults and genitalia of the species are provided. Specific distinction was supported by the COI barcode study.

• 설비공학논문집
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• 제23권2호
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• pp.164-171
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• 2011

The objectives of this study are to propose a suitable treatment facility for waste energy recovery after analyzing the waste generation and disposal situation in Jejudo, to establish the plan to install the solar photovoltaics and wind power plant considering the site conditions and finally to establish the environmental energy town plan in conjunction with the existing facilities. The food waste biogas plant is selected as the treatment capacity of 200 ton/day. It is estimated that the biogas plant will produce the electricity of 7,594 MWh per year, which will reduce the greenhouse gas of 4,177 $tCO_2$ per year. The solar photovoltaics and wind power plant will produce the electricity of 13,410 MWh per year, which will reduce the greenhouse gas of 7,375 $tCO_2$ per year. Environmental energy town will give us the reduction of operating cost by centralized treatment of residues and byproducts, and by efficient utilization of produced energy.

• The Plant Pathology Journal
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• 제27권1호
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• pp.8-13
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• 2011

The induced resistance of cucumber leaves treated with oligochitosan to the infection of the cucumber powdery mildew, Sphaerotheca fuliginea, was investigated using transmission electron microscopy. The results showed that when the plants were treated with oligochitosan and challenged with inoculum, a significant decrease of the disease occurred. The mycelial development in the treated leaves was markedly inhibited. The cytoplasm of the powdery mildew mycelium was aggregated, with its organelles disintegrated and the cytoplasm collapsed. The protoplasm in haustoria became electron-dense. Haustoria became malformed, their organelles disintegrated, the hausterial wall thickened and eventually the whole complex necrotized. The host cells produced defence structures and materials associated with infection and a hypersensitive response. The host cell wall was thickened and deeply stained; several layers of papilla structure were produced under the cell wall; dark materials were deposited between the cell wall and plasmalemma; extrahaustorial plasmalemma was deeply stained and extrahaustorial matrix appositions had large deposits of electron-dense material; the cytoplasm was disordered, host organelles disintegrated and eventually the whole host cell disintegrated and necrotized.

• 국제학술발표논문집
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• The 6th International Conference on Construction Engineering and Project Management
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• pp.667-668
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• 2015

While the plant projects grow bigger and global attention to the plant is increasing, efficient space arrangement is not working in plant project because of the complex structure in installing the equipment unlike the construction project. In addition to this, presently, problem in installation process caused by the disagreement between floor plan and real spot is rising. Therefore the target of this research is to solve the problems and reaction differences, caused by changing the space arrangement in installing the equipment of plant construction. And this research suggests the equipment arrangement method for construction and related processes. To solve the problem, 3D cloud point data of space and equipment is collected by 3D laser scanning and the space matching is operated. In processing the space matching, data is simplified by applying the octree algorithm. This research simplifies the 3D configuration data acquired by 3D scanner equipment through the octree algorithm, and by comparing this data, identifies the space for target equipment, and finally suggests the algorithm that makes the auto space arrangement of equipment possible in construction site and also suggests the process to actualize this algorithm.

• 한국유기농업학회지
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• 제19권spc호
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• pp.17-20
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• 2011

Plant biomass is a huge carbon-complex that has potential as a nutrient. Therefore we extracted and separated useful materials for plant growth from tea leaf and stem. The pre-treatment process including high temperature ($200^{\circ}C$) and pressure (20-40 kgf/$cm^2$) was treated for several minutes and extracted at $120^{\circ}C$ for 30-60 minutes. After that the chemical compositions and ingredients were analyzed from that plant-nutrient. As a result of mineral contents, calcium and magnesium concentrations are higher than other minerals. Also the result of carbohydrates analyses has shown that the sugar oligomer consists of xylose(95.3%) and glucose(4.7%), and the sugar monomer consists in the order of xylose (52.7%) > manose (22.8%) > arabinose (10.8%) > galactose (10.2%) > glucose (3.5%). Before applied to field, in vitro plant growth system and formulation were examined. To evaluate the effect of the nutrients, both strawberry green-house and persimmon fields were used in this test. The treated persimmons were heavier than controls scored at 13-22%. In addition, the storage-period was extended in the treated strawberries. Interestingly in the treated strawberry, the contents of polyphenols were increased (38-57%). These results suggest that the plant-nutrient can afford to help for plant growth and storage, and it can be substituted for other commercial nutrients. In conclusion, this plant-nutrient may help to extend eco-friendly or organic farming in Hadong-gun area.

• The Plant Pathology Journal
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• 제35권5호
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• pp.521-529
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• 2019

Tomato yellow leaf curl China virus is a species of the widespread geminiviruses. The infection of Nicotiana benthamiana by Tomato yellow leaf curl China virus (TYLCCNV) causes a reduction in photosynthetic activity, which is part of the viral symptoms. ${\beta}C1$ is a viral factor encoded by the betasatellite DNA ($DNA{\beta}$) accompanying TYLCCNV. It is a major viral pathogenicity factor of TYLCCNV. To elucidate the effect of ${\beta}C1$ on plants' photosynthesis, we measured the relative chlorophyll (Chl) content and Chl fluorescence in TY-LCCNV-infected and ${\beta}C1$ transgenic N. benthamiana plants. The results showed that Chl content is reduced in TYLCCNV A-infected, TYLCCNV A plus $DNA{\beta}$ (TYLCCNV A + ${\beta}$)-infected and ${\beta}C1$ transgenic plants. Further, changes in Chl fluorescence parameters, such as electron transport rate, $F_v/F_m$, NPQ, and qP, revealed that photosynthetic efficiency is compromised in the aforementioned N. benthamiana plants. The presense of ${\beta}C1$ aggravated the decrease of Chl content and photosynthetic efficiency during viral infection. Additionally, the real-time quantitative PCR analysis of oxygen evolving complex genes in photosystem II, such as PsbO, PsbP, PsbQ, and PsbR, showed a significant reduction of the relative expression of these genes at the late stage of TYLCCNV A + ${\beta}$ infection and at the vegetative stage of ${\beta}C1$ transgenic N. benthamiana plants. In summary, this study revealed the pathogenicity of TYLCCNV in photosynthesis and disclosed the effect of ${\beta}C1$ in exacerbating the damage in photosynthesis efficiency by TYLCCNV infection.

• 한국지반공학회논문집
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• 제38권10호
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• pp.49-60
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• 2022

지상공간의 건설부지 부족 현상으로 인한 도심지 지하화가 진행되고 있으며, 이에 따른 건설부지의 정확한 지반특성 평가가 요구되고 있다. 본 논문에서는 지하공간을 활용한 복합플랜트 후보부지에 대한 지반특성 파악하고자 시추조사와 다양한 물리탐사 방법 중 지표 탐사인 탄성파 굴절법 탐사와 전기비저항 탐사를 수행하였다. 시추조사는 BH-1 ~ BH-9까지 총 9개 시추공에서 수행하였으며, 일정한 간격을 유지하여 각 공에서의 지하수위 및 지질구조의 수직분포 등에 대한 정보를 획득하였다. 탄성파 굴절법 탐사에서 각 지층의 구간별 속도 및 지층 두께를 측정하였으며, 전기비저항 탐사는 쌍극자 배열법을 이용하여 지층의 전기비저항 분포를 파악하였다. 시추조사 결과를 종합하여 대상지반의 토사층 분류 및 기반암선을 파악하였으며, 물리탐사 결과를 통해 파쇄대와 같은 이상대를 평가하였다. 본 논문은 시추조사와 물리탐사 기법을 활용하여 지하복합 플랜트 후보부지에 대한 지반특성 파악하였고, 향후 시추조사와 물리탐사를 병행하는 지반조사에 참고 자료로 쓰일 수 잇다는 점에서 의의가 있다.

• Journal of Photoscience
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• 제9권2호
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• pp.82-85
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• 2002

33kDa extrinsic protein, an important protein in oxygenic photosynthesis, was known to have no fixed configuration in solution. At 20$\^{C}$ and pH 6, 33kDa extrinsic protein showed changes of free energy of -14.6 kJ/mor$\^$-1/ and of standard volume of -120mL/mol, respectively, with increase of hydrostatic pressure, comparatively lower than for most proteins. NBS modification of Trp241 in 33kDa extrinsic protein dramatically changes the secondary protein structure, its affinity to photosystem II as well as photosynthetic oxygen evolution. The relationship between structural change and transport of oxygen, water and proton is deserved a further study.