• Journal of Microbiology and Biotechnology
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• v.11 no.2
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• pp.204-210
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• 2001

Paclitaxel can be produced in high yield and with a high degree of purify from plant cell cultures of Taxus chinensis. The complete purification method was systematically established and described. This method was an efficient procedure for the purification of paclitaxel from crude paclitaxel, consisting or reverse-phase chromatography, followed by a normal-phase chromatography. The two-stage HPLC purification scheme serves as an effective and economical approach for resolving paclitaxel from complex mixtures of taxoids, with high purify (>99%) and low impurities (<0.1%). The process is readily scalable to a pilot plant and eventually to a production environment where multikilogram quantities of material are expected to be produced. The process has been optimized to minimize solvent usage, complexity, and operating costs.

• Tunnel and Underground Space
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• v.9 no.3
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• pp.175-184
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• 1999

Systematic measurements in the field are the key component in the design process to ensure that optimal and safe designs result. The instruments installed at the Waste Isolation Pilot Plant, a underground nuclear waste repository in U.S., for measuring rock deformation was reviewed. Also discussions about installation and measurement for better understanding the complex time-dependent deformational behavior of underground excavation were made.

• Proceedings of the Korea Committee for Ocean Resources and Engineering Conference
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• 2006.11a
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• pp.269-272
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• 2006

The use of packaged skid in oil and gas plant has increased dramatically over the decades, replacing loose supply equipment. Most equipment is installed on structural skid in manufacturer's shop. As Oil and Gas Plants are constructed at inaccessible area, the demands of packaged skids are increased. The packaged skid have advantages comparing to loose supply equipment in inaccessible area. The packaged skid is small complex plant consists of process, mechanical, piping, instrument, electrical and structural steel. This paper examines advantages and problems of packaged skid, based on previous experience. EPC company consider key factors such as manufacturing period, construction periods, equipment quality and costs.

• Journal of Advanced Marine Engineering and Technology
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• v.27 no.3
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• pp.388-394
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• 2003

Hydraulic system is difficult to obtain a suitable performance due to the nonlinearity load pressure change and system parameter variation. The requirement of control a1gorithm has been complex in order to satisfy the performance. The adaptive control is a control method which is suggested to achieve the control object under the plant characteristics change. In spite of the case that plant characteristics and the degree of variation are difficult to grasp. the adaptive control could keep the characteristics of closed-loop system generally. In this study. a method of combined generalized minimum variance adaptive control (GMVAC) and output error feedback is proposed, in order to solve the problem of non-minimum phase of plant and the vibration and overshoot in initial response. The control performance according to the variation of characteristics of plant is evaluated by changing the supply pressure. The experimental results show the effectiveness of the proposed scheme.

• The Plant Pathology Journal
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• v.20 no.1
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• pp.22-29
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• 2004

Many important horticultural and field crops are susceptible to virus infections or may possess a degree of resistance to some viruses, but become infected by others. Plant viruses enter cells through the presence of wounds, and replicate intracellularly small genomes that encode genes required for replication, cell-to-cell movement and encapsidation. There are numerous evidences from specific virus-host interactions to require the involvement of host factors and steps during viral replication cycle. However, viruses should deal with host defense responses either by general or specific mechanisms, targeting viral components or genome itself. On the other hand, the host plants have also adapted to defend themselves against viral attack by operating different lines of resistance responses. The defense-related interactions provide new insights into the complex molecular strategies for hosts for defense and counter-defense employed by viruses.

• Journal of Microbiology and Biotechnology
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• v.19 no.6
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• pp.573-581
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• 2009

The complex enzyme pool secreted by the phytopathogenic fungus Fusarium graminearum in response to glucose or hop cell wall material as sole carbon sources was analyzed. The biochemical characterization of the enzymes present in the supernatant of fungal cultures in the glucose medium revealed only 5 different glycosyl hydrolase activities; by contrast, when analyzing cultures in the cell wall medium, 17 different activities were detected. This dramatic increase reflects the adaptation of the fungus by the synthesis of enzymes targeting all layers of the cell wall. When the enzymes secreted in the presence of plant cell wall were used to hydrolyze pretreated crude plant material, high levels of monosaccharides were measured with yields approaching 50% of total sugars released by an acid hydrolysis process. This report is the first biochemical characterization of numerous cellulases, hemicellulases, and pectinases secreted by F. graminearum and demonstrates the usefulness of the described protein cocktail for efficient enzymatic degradation of plant cell wall.

• Environmental Engineering Research
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• v.10 no.2
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• pp.88-103
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• 2005

In this paper, data-driven modeling and multiresolution analysis (MRA) are applied for a full-scale wastewater treatment plant (WWTP). The proposed method is based on modeling by partial least squares (PLS) and multiscale monitoring by a generic dissimilarity measure (GDM), which is suitable for nonstationary and non-normal process monitoring such as a biological process. Case study in an industrial plant showed that the PLS model could give good modeling performance and analyze the dynamics of a complex plant and MRA was useful to detect and isolate various faults due to its multiscale nature. The proposed method enables us to show the underlying phenomena as well as to filter out unwanted and disturbing phenomena.

• Korean Journal of Plant Resources
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• v.35 no.3
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• pp.405-410
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• 2022

We have discovered Vicia grandiflora Scop., a newly invasive alien species in Baekun-ri, Okcheon-gun, Chungcheongbuk-do, Korea. This species is native to regions from Central and Southeast Europe to Central Asia and Iran and is reported as an invasive species in North America and Japan. This species is similar to the Vicia sativa complex (V. sativa subsp. sativa, V. sativa subsp. nigra) but can be readily distinguished by the undivided ovate to semi-hastate stipules of the upper leaves, yellowish petals, large size of its flower, and elongated hilum. In the field, V. grandiflora grows in disturbed sites near cultivated land, suggesting that their seeds are typically transported by vehicles along with fertilizer or livestock feed. Here, we present the morphological description, photographs, and sites of V. grandiflora growth, which will be useful in guiding the management of this invasive alien plant.

• Journal of the Korean Society of Systems Engineering
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• v.12 no.1
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• pp.89-103
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• 2016

Due to safety of the plant, it became very clear the importance of study occurrence reactor coolant system (RCS) issues specially the primary water stress corrosion cracking (PWSCC). The Systems Engineering (SE) approach is characterized by the application of a structured engineering methodology for the design of a complex system or component. Robotic devices have been used for internal inspection, maintenance and performing remote welding and inspection in high-radiation areas. In this paper, PWSCC overview and inlay and over lay welding methodology introduced, concept of robotic device that can be inserted into the piping via Steam Generator (SG) main way to access to primary piping of pressurized water reactor (PWR) is developed based on SE methodology. A 3D model of the inspection system was developed along with the APR1400 (Advanced Power Reactor)reactor coolant systems (RCS) and internals with virtual 3D simulation of the operation for visualization to prove the validity of the concept.

• The Plant Pathology Journal
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• v.30 no.2
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• pp.151-158
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• 2014

Three double-stranded RNAs (dsRNAs), approximately 1.85, 1.65 and 1.27 kb in size, were detected in an isolate of Cytospora sacchari from Iran. Partial nucleotide sequence revealed a 1,284 bp segment containing one ORF that potentially encodes a 405 aa protein. This protein contains conserved motifs related to RNA dependent RNA polymerases (RdRp) that showed similarity to RdRps of partitiviruses. The results indicate that these dsRNAs represent a novel Partitivirus that we tentatively designate Cytospora sacchari partitivirus (CsPV). Treatment of the fungal strain by cyclohexamide and also hyphal tip culture had no effect on removing the putative virus. Phylogenetic analysis of putative RdRp of CsPV and other partitiviruses places CsPV as a member of the genus Partitivirus in the family Partitiviridae, and clustering with Aspergillus ochraceous virus 1.