• Reproductive and Developmental Biology
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• v.32 no.4
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• pp.249-253
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• 2008

Interspecies somatic cell nuclear transfer (iSCNT) is a valuable tool for studying the interactions between an oocyte and somatic nucleus. The object of this study was to investigate the developmental competence of in vitro-matured porcine oocytes after transfer of the somatic cell nuclei of 2 different species (goat and rabbit). Porcine cumulus oocytes were obtained from the follicles of ovaries and matured in TCM-199. The reconstructed embryos were electrically fused with 2 DC pulses of 1.1kV/cm for $30{\mu}s$ 0.3M mannitol medium. The activated cloned embryos were cultured in porcine zygote medium-3 (PZM-3), mSOF or RDH medium for 7 days. The blastocyst formation rate of the embryos reconstructed from goat or rabbit fetal fibroblasts was significantly lower than that of the embryos reconstructed from porcine fetal fibroblast cells. However, a significantly higher number of embryos reconstructed from goat or rabbit fetal fibroblasts cultured in mSOF or RDH, respectively, developed to the morular stage than those cultured in PZM-3. These results suggest that goat and bovine fetal fibroblasts were less efficacious than porcine-porcine cloned embryos and that culture condition could be an important factor in iSCNT. The lower developmental potential of goat-porcine and porcine-bovine cloned embryos may be due to incompatibility between the porcine oocyte cytoplasm and goat and bovine somatic nuclei.

• The Research Journal of the Costume Culture
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• v.24 no.3
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• pp.385-398
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• 2016

In a world where opportunities and chances are common, the phenomenon referred to as "generation maybe" describes people in their 20s and early 30s who are usually being indecisive. With the increase in breadth of information and choices, the number of people who are hesitant in deciding whether to purchase increases at a fast pace, as does the number of products and services targeting this group. In order to enhance our understanding of the phenomenon of consumer hesitation, this study explores contextual factors and consumer characteristics that affect consumer hesitation in the purchase decision-making process. Specifically, this study examines whether seven contextual factors, self-determination tendencies, and five decision-making styles influence consumer hesitation in the product decision making process. An online survey was administered to test our research questions. A total of 309 Korean consumers (female=48.9%) aged from 20 to 35 were surveyed. A regression analysis revealed that four contextual factors (product involvement, need for information on alternatives, relative price, and uncertain need), two sub-dimensions of self-determination (perceived competence and relatedness), and two decision-making styles (price seeking and advice seeking) have noticeable influences on consumer hesitation. We also found that the degree of consumer hesitation positively influences consumer post-purchase satisfaction. The study concludes with discussions and practical implications.

• Journal of Home Health Care Nursing
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• v.27 no.1
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• pp.42-52
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• 2020

Purpose: This study aimed to find grounds for the development of a health promotion program by examining the risk factors affecting children in low-income families. Methods: This was a cross-sectional study. The subjects were 288 children under 13 years of age in We-Start, W city. The tools used included a household information questionnaire and risk assessment tools. The data were analyzed using t-test, ANOVA, and multiple regressions. Results: Risk factors like disability problems (β=.38, p<.001), residential environment (β=.37, p<.001), parenting type (β=.27, p=.003), the foster's educational level (β=.22, p=.011), and the gender of the child (β=.19, p=.030) explained 51% (p<.001) of the preschoolers in crisis. For the schooler, academic achievement (β=.39, p<.001), disability problems (β=.24, p<.001), adaptation to school (β=.23, p<.001), noise from the environment (β=.20, p<.001), and the foster's job (β=-.15, p=.007) explained 50% (p<.001) of crisis of schooler. Conclusion: The study found that children (disability, gender), family (residential environment, type of parenting), and caregivers (educational level, economic competence) had a complex impact on crisis situations. In children of a school-going age, school life appeared to be an important influencing factor. Therefore, an integrated case management approach that considers children, carers, and the home environment is necessary.

• Journal of the Korean Home Economics Association
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• v.36 no.9
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• pp.49-62
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• 1998

This paper investigates the parenting stress of fathers who have preschooler. At first, a causal model constructed, including the parenting stress as a dependent variable and the psychological characteristics of paternal self-esteem and parenting efficacy as parameters. Based on the change of parenting stress, self-esteem and parenting efficacy according to various pertinent to fathers, the causal effect of various variables effecting on the paternal parenting stress is analyzed. The SPSS PC program was used for the statistical analysis, where fully exploited were frequencies, percentages, means, standard deviations, Cronbach α, one-way analysis(ANOVA), path analysis. The results can be summarized into 3 parts, in conclusion: 1) The younger a father is, the higher paternal self-esteem becomes. Paternal self-esteem trend was there, for a father who is a member of large family, and for a father whose education level is high, and for a father whose occupation is professional. Regarding parenting sense of competence, a father shows confidence when he has more income, more educated and less children. 2) The variable analysis effecting on the parenting stress dut to the paternal environment shows that a father feels more stress when his income is less and his spouse is working. The sub-factor analysis on the parenting stress shows that 'typical stress dut to the parenting' increases when his income is less and his spouse is working, and 'pressures pertaning to the parental role and distress' increases for a father with less income, working spouse, less educated and non-professional job. Further 'guilty to the parenting by others' shows higher for a father with less income and a working spouse. 3) The path analysis, which were performed to check the causality of the parenting stress dut to various variables, shows that the self-esteem and the parenting efficacy are significant factors directly effecting on the paternal parenting stress, and the job-satisfaction and marriage-satisfaction are significantly effecting on the parenting stress both directly and indirectly.

• Asian-Australasian Journal of Animal Sciences
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• v.21 no.3
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• pp.358-363
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• 2008

The intracytoplasmic sperm injection (ICSI) procedure has recently been utilized to produce transgenic animals and may serve as an alternative to the conventional pronuclear microinjection in species such as pigs whose ooplasm is opaque and pronuclei are often invisible. In this study, the effects of sperm membrane disruption and electrical activation of oocytes on in vitro development and expression of transgene green fluorescent protein (GFP) in ICSI embryos were tested to refine this recently developed procedure. Prior to ICSI, sperm heads were treated with Triton X-100+NaCl or Triton X-100+NaCl+NaOH, to disrupt membrane to be permeable to exogenous DNA, and incubated with linearized pEGFP-N1 vector. To induce activation of oocytes, a single DC pulse of 1.3 kV/cm was applied to oocytes for $30{\mu}sec$. After ICSI was performed with the aid of a micromanipulator, in vitro development of embryos and GFP expression were monitored. The chemical treatment to disrupt sperm membrane did not affect the developmental competence of embryos. 40 to 60% of oocytes were cleaved after injection of sperm heads with disrupted membrane, whereas 48.6% (34/70) were cleaved without chemical treatment. Regardless of electrical stimulation to induce activation, oocytes were cleaved after ICSI, reflecting that, despite sperm membrane disruption, the perinuclear soluble sperm factor known to mediate oocyte activation remained intact. After development to the 4-cell stage, 11.8 (2/17, Triton X-100+NaCl+NaOH) to 58.8% (10/17, Triton X-100+NaCl) of embryos expressed GFP. The expression of GFP beyond the stage of embryonic genome activation (4-cell stage in the pig) indicates that the exogenous DNA might have been integrated into the porcine genome. When sperm heads were co-incubated with exogenous DNA following the treatment of Triton X-100+NaCl, GFP expression was observed in high percentage (58.8%) of embryos, suggesting that transgenic pigs may efficiently be produced using ICSI.

• Journal of Families and Better Life
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• v.23 no.5 s.77
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• pp.133-143
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• 2005

The purpose of this study was to gain understanding about residential satisfaction and preference of the members of the future elderly generation who have an intention to develop senior congregate housing. The data for this study were collected from 600 participants who were in their 40s to 60s, residing in Seoul, and who have an intention to develop senior congregate housing. The data collection spanned from December 1st to 20th, 2004. The results of this study were as follows: Firstly, the respondents who had a higher level of education, higher income, and a high-ranking job showed relatively higher residential satisfaction with their present house. Similar satisfaction level came between their present house and expecting ones in their later life. Financial responsibility was the most serious expected problem when they continue living in the present house in the later life. It means that the economic ability is the primary factor that determines whether they will have a stable life later. Secondly, as for the preference for the senior housing developer type, the group who preferred collectively developed senior congregate housing far outnumbered the other who preferred independent developer type. Also, the group who preferred collective developer type had higher economic ability than that of the other. These results indicate that above all, economic competence is the must significant variable, which should be considered in the future life and residential planning for the elderly. Korean senior congregate housing models should be developed based upon elaborate research. It is also necessary to develop different types of housing, such as a more practical type and a high quality type, as well as to respond to different kinds of developmental objectives, such as marketability and public benefit.

• Korean Journal of Construction Engineering and Management
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• v.7 no.3 s.31
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• pp.76-85
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• 2006

Quality is the core competence for customer satisfaction in current competitive business environments. The manufacturing companies regard the quality as the success factor in enhancing competitiveness and foremost concept of the management innovation. But in many cases, the basis for the quality management and the action programs are not prepared yet. In construction industry, normally schedule and cost have priority over quality and the level of overall quality is relatively lower than other industries. This is caused by the vague quality goal and result-oriented management. This study suggests the quality performance indicators for measuring performance objectively, and develops the continuous quality monitoring system based on those indicators. By using this system, the quality improvement can be expected and corporate quality competitiveness can be ensured.

• Korean Journal of Human Ecology
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• v.20 no.1
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• pp.257-271
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• 2011

The purpose of this study is to provide basic data that is necessary for planning an adequate environment for various child-care activities and programs by recognizing the environmental characteristics of a group room, where children's activities take place. A survey was conducted of 257 childcare centers and 544 teachers all over the country, including only those of which that have opened after year 2000 with more than 20 children capacities. The survey period was from January 18th to 28th of 2010. The data was analyzed using SPSS WIN 15 software, calculating the data's frequency, percentage, $x^2$, multiple response analysis, F-test, and factor analysis. The results of this study led us to the following conclusions. 1) We would like to propose a group room space design varying by the room size, so that interesting and diverse activities can take place after taking account of the child's development and childcare centers program. 2) Group room space and facilities should be made of proper finishing materials, various materials with differing textures, and lighting for the kinds of childcare activities that gives various sensational experiences and comfortable feelings to the children. 3) Teachers with higher age and education tend to believe that child's competence and a homelike atmosphere are important factors in a group room environment.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.16 no.1
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• pp.354-364
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• 2015

This study examined the effect of sociocultural attitudes toward the appearance of depression and psychological well-being of fitness in adult women. The subjects of this study consisted of 472 healthy adult woman. The used instruments were Jung (2011) for measuring the sociocultural attitudes toward appearance scale, Seo (2006) for measuring the depression scale, and Yang (2000) for measuring the psychological well-being scale. The data was analyzed by exploratory factor analysis, reliability analysis, correlation analysis and multiple regression analysis. The results are as follows. First, among the subscales of sociocultural attitudes toward appearance, stress positively influenced depression. Second, among the subscales of sociocultural attitudes toward appearance, internalization-general negatively influenced hedonic enjoyment, self-realization, and a feeling of flow, but information positively influenced it. In addition, internalization-athlete positively influenced the feeling of competence, self-realization. Third, depression negatively influenced hedonic enjoyment, self-realization, and the feeling of flow.

• The Journal of the Korea Contents Association
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• v.17 no.4
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• pp.83-94
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• 2017

This study aimed to provide important information on vocational education and employment policy for the disabled by analyzing the relationship between disabled wage workers' occupational ability and job quality. For this purpose, the relationship between job competence and employment quality was theoretically examined and empirically analyzed based on human capital theory, screening theory, and job competition theory. The empirical analysis used data on 975 disabled wage workers with the use of the data of the 8th Panel Survey of Employment for the Disabled(2015). As a result of the empirical study, it was found that the occupational ability of the disabled workers is an important factor in determining Wage, job status, job satisfaction, which constitute the quality of employment. This result indicates that job quality can be improved by ameliorating disabled people's occupational ability. Accordingly, this study proposed that there might be a need to extend customized education and educational facilities for improving disabled people's occupational ability.