• 한국정보통신학회논문지
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• 제20권12호
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• pp.2274-2281
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• 2016

장기생태연구 커뮤니티에서 개인정보는 국가단위 뿐만 아니라 국제장기생태연구의 데이터 관리, 데이터 활용에서 협업을 위한 중요한 요소이다. 개인정보를 많은 부분 공개를 하면 협업 연구자에게 데이터 접근 측면에서 유용하지만, 정보 제공자에게는 공개에 대한 부담이 되기도 한다. 개인의 정보보호 관련 법률에서는 개인정보의 수집범위와 유통측면에서 많은 제약 요소가 존재하여 장기생태정보시스템에서도 장기생태연구 협업에 필요한 최대의 개인정보와 비 관련자에게 제공하는 최소의 개인정보 제공이 고려되어야 한다. 본 연구는 국내외 데이터 교류를 위해 관련 법률의 해석을 기반으로 국제 메타데이터 표준인 EML과 국제 장기생태 플랫폼의 개인정보 관리에 대한 동향을 분석하고 개인정보 관리를 위한 시스템 모델을 설계 및 구현을 통해 제시하고자 한다.

• 한국지하수토양환경학회:학술대회논문집
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• 한국지하수토양환경학회 2004년도 임시총회 및 추계학술발표회
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• pp.175-178
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• 2004

Little study has been published specifically addressing the dynamics of nitrate reducing bacteria (NBR) during the bioremediation of nitrate-contaminated aquifer. In our previous study we successfully quantified fumarate-enhanced microbial nitrate reduction rate in a nitrate-contaminated aquifer by using a series of single-well push-pull tests (PPTs). In this study we analyzed the suspended population during PPTs. To monitor changes in the microbial community, PCR amplification of 16S rDNA genes and denaturing gradient gel electrophoresis (DGGE) were used to study the dynamics of the bacterial community in detail. Before the stimulation of NBR, the dominant DGGE bands obtained by PCR were affiliated with V-Proteobacteria consisting of Acinetobacter spp. and Pseudomonas fluorescens. However, as NBR biostimulation proceeded, the dominant patterns of DGGE bands changed, and they were affiliated with Azoarcus denitrificans Td-3 and Flavobacterium xanthum. Azoarcus denitrificans Td-3 is known to completely reduce nitrate to nitrogen gas. The series of single-well push-pull tests in this study should prove useful for conducting rapid, low-cost feasibility assessments for in situ denitrification and provide important information about which microorganisms play a key role in bioremediation of a nitrate contaminated aquifer.

• Journal of Microbiology and Biotechnology
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• 제31권6호
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• pp.803-814
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• 2021

A pilot-scale biocover was constructed at a sanitary landfill and the mitigation of methane and odor compounds was compared between the summer and non-summer seasons. The average inlet methane concentrations were 22.0%, 16.3%, and 31.3%, and the outlet concentrations were 0.1%, 0.1%, and 0.2% during winter, spring, and summer, respectively. The odor removal efficiency was 98.0% during summer, compared to 96.6% and 99.6% during winter and spring, respectively. No deterioration in methane and odor removal performance was observed even when the internal temperature of the biocover increased to more than 40℃ at midday during summer. During summer, the packing material simultaneously degraded methane and dimethyl sulfide (DMS) under both moderately thermophilic (40-50℃) and mesophilic conditions (30℃). Hyphomicrobium and Brevibacillus, which can degrade methane and DMS at 40℃ and 50℃, were isolated. The diversity of the bacterial community in the biocover during summer did not decrease significantly compared to other seasons. The thermophilic environment of the biocover during summer promoted the growth of thermotolerant and thermophilic bacterial populations. In particular, the major methane-oxidizing species were Methylocaldum spp. during summer and Methylobacter spp. during the non-summer seasons. The performance of the biocover remained stable under moderately thermophilic conditions due to the replacement of the main species and the maintenance of bacterial diversity. The information obtained in this study could be used to design biological processes for methane and odor removal during summer and/or in subtropical countries.

• 한국농공학회논문집
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• 제64권1호
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• pp.27-37
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• 2022

This study established a water supply network based on the operation case of Mandae Reservoir in Yanggu-gun, Gangwon-do, to analyze the efficient distribution and management of agricultural water supplied from the reservoir to irrigation areas using the hydraulic analysis model SWMM. In order to construct a model to analyze the water canal network, network conditions needs to be simplified, and in particular, excessive detail or simplification of the irrigation area can lead to errors in the analysis results. Therefore, the effect of the water canal network model was analyzed by simulating the appropriate simplification process step by step. The results of simplifying the actual block shape of the analysis target area using SWMM showed that there was no significant difference in the results even if 7 lots were simplified to 2. Also, it was found that the construction and analysis of a simplified network model were reliable when the excess quantity was 2% or more compared to the required quantity for each case of analysis of the paddy field.

• 한국농공학회논문집
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• 제66권3호
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• pp.39-51
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• 2024

To effectively implement the integrated water management policy outlined in the National Water Management Act, it is essential to analyze agricultural water supply and demand at both basin and water district levels. Currently, agricultural water is primarily distributed through open canal systems and controlled by floodgates, yet the utilization-to-supply ratio remains at a mere 48%. In the case of agricultural water, when analyzing water balance through existing national basin water resource models (K-WEAP, K-MODISM), distortion of supply and regression occurs due to calculation of regression rate based on the concept of net water consumption. In addition, by simplifying the complex and diverse agricultural water supply system within the basin into a single virtual reservoir, it is difficult to analyze the surplus or shortage of agricultural water for each field within the basin. There are limitations in reflecting the characteristics and actual sites of rural water areas, such as inconsistencies with river and reservoir supply priority sites. This study focuses on the development of a model aimed at improving the deficiencies of current water balance analysis methods. The developed model aims to provide standardized water balance analysis nationwide, with initial application to the Anseo standard watershed. Utilizing data from 32 facilities within the standard watershed, the study conducted water balance analysis through watershed linkage, highlighting differences and improvements compared to existing methods.

• 농촌계획
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• 제22권3호
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• pp.119-129
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• 2016

The aim of this study is to suggest the quantitative standard of a marginal village. For the purpose, the study selected 43 villages with the population of 50 or less, the classification of a marginal village in accordance with advanced research and 47 villages with the population over 50 in Buyeo-gun region. The common reason of occurrence of a marginal village suggested by advanced research is a characteristic of a village or decline and extinction of a village rather than a simple index of population or aging rate. Therefore, the study assumed that decline of functions of the village would be caused by decline a function of communities consisting of the villagers. The study then assumed that the relatively low or 0 number or rate of participants would result in community functions. The study conducted t-test on basis of population and aging rate and an analysis to find the range with relatively large differences in the number of communities, participants and the rate of the participants, etc. The result showed that the community function began to decline when the population was less than 60~70 and the aging rate over 75%~85%. As the decline of functions of communities began in population of 70, the critical point was met when the population was 40 or less. With population of 40 or less, the young and the old group communities became extinct or showed rapid decrease in the number of participants. The study assumed that decline of functions of a village, a reason of occurrence of a marginal village would be decline of functions of communities, but there was no further analysis on decline or extinction of a village with population of 40 or less. There shall be further studies about whether a village of population of 40 or less is led to decline of a function or extinction of village communities.

• 미생물학회지
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• 제46권4호
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• pp.352-358
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• 2010

서로 다른 처리공정으로 운영되는 4개의 하수시설을 대상으로 16S rRNA 유전자 기반의 pyrosequencing을 이용해 활성슬러지 하수처리 생물반응기의 세균군집구조를 분석하였다. 활성 슬러지에는 Rhodocyclales, Burkholderiales, Sphingobacteriales, Myxococcales, Xanthomonadales, Acidobacteria group 4, Anaerolineales, Methylococcales, Nitrospirales, Planctomycetales 목에 속하는 염기서열이 전체의 54-68%를 차지해, 소수의 세균 분류군이 활성슬러지 세균군집의 대부분을 차지하고 있었다. 이들 소수 세균 분류군의 조성은 처리장별로 차이가 있었으며, 하수처리장의 운전조건 및 환경조건에 영향을 받는 것으로 추측되었다. 또한, 활성슬러지는 매우 다양한 세균 종을 가지는 것으로 관찰되었는데(Chao1 richness estimate: 1,374-2,902 operational taxonomic units), 대부분의 다양성은 희귀 종에 기인한 것으로 나타났다. 특히, 분리막으로 운영되는 하수처리시설에서 높은 다양성을 나타내었는데, 처리공정이 매우 긴 고형물체류시간으로 운영되어 느리게 성장하는 다양한 세균이 서식하는데 용이하기 때문인 것으로 판단되었다. High-throughput pyrosequencing 기술을 이용하여 활성슬러지 세균군집을 처리장별로 비교 분석한 본 연구는 향후 활성슬러지 미생물의 생태학적 특성을 보다 잘 이해하고 하수처리공정을 개선하는데 도움이 될 것이다.

• 환경생물
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• 제37권4호
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• pp.600-606
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• 2019

본 연구는 2018년 7월 20일부터 2018년 8월 1일까지 안산시 단원구에 위치한 화랑유원지 오토캠핑장의 집수정에서 연구를 실시하였으며, 조사지점은 기법별로 대조지점, 천적지점, Bti 지점을 선정하였다. 서식처 환경 분석 및 채집된 저서성 대형무척추동물을 대상으로 종조성, 군집분석, 상관성 분석, 유사도 분석을 실시하여 잔물땡땡이, Bti가 저서성 대형무척추동물에 미치는 영향을 파악하였다. 서식처 환경 분석 결과, 수질항목은 지점간 유의한 차이는 없는 것으로 분석되었으며(p>0.05), 하상구조 및 식물군락이 유사하여 서식처 특성에 의한 종조성의 차이는 적을 것으로 판단된다. 조사시간 동안 총 11목 22과 38종 4,818개체가 출현하였으며, 천적지점은 대조지점과 비교적 유사한 종조성을 나타내는 것으로 분석되었다. 반면 Bti 지점은 다른 지점에 비해 단순한 종조성을 나타내었으며, 파리목의 종수 및 개체수가 가장 빈약한 것으로 분석되었다. 군집 분석 결과, 조사기간 동안 Bti 지점에서 불안정한 군집양상을 나타내었으며, 천적지점은 비교적 안정적인 군집양상을 나타내는 것으로 분석되었다. 파리목과 모기유충을 대상으로 수질과의 상관성 분석을 실시한 결과, 파리목과 기온, 수온은 음의 경향성을 나타내었으며, 모기유충과 기온은 -0.610^⁎, 수온은 -0.674^⁎로 유의한 음의 상관성을 나타내는 것으로 분석되었다 출현종을 대상으로 유사도 분석을 실시한 결과, 대조지점과 천적지점은 조사시기별 61.11~73.68%, Bti 지점은 30.77~56.00%의 유사도를 나타내어 천적지점에 비해 대조지점과 유사도가 낮은 것으로 분석되었다. 본 연구는 단기간의 연구 결과로 습지유형 등을 고려하여, 다양한 환경에서 생물학적 모기유충 방제 기법이 저서성 대형무척추동물에게 미치는 영향에 대한 장기적인 연구가 필요할 것으로 판단된다.

• Journal of Microbiology and Biotechnology
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• 제15권2호
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• pp.287-295
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• 2005

In order to compare bacteria] community structure and diversity in activated sludges, terminal restriction fragment length polymorphism (T-RFLP) of PCR-amplified 16s rDNAs was analyzed for 31 domestic and industrial wastewater treatment plants (WTPs). Regardless of the characteristics of the wastewaters, the bacteria] community structures of activated sludges appeared diverse and complex. In particular, activated sludges in domestic WTPs contained higher bacterial diversity than those in industrial WTPs. It was also found that terminal restriction fragment (T-RF) profiles derived from domestic WTPs were very similar with each other, although activated sludges were collected from different plants at different locations. Interestingly, activated sludges of a WTP where restaurant and toilet sewages of a company were managed showed a bacterial community structure similar to that of domestic WTPs. Activated sludges in leather industria] WTPs also showed a high similarity. However, other wastewaters possessed different bacterial communities, so that overall similarity was as low as about $30\%$. Since activated sludges from WTPs for domestic wastewaters and a company sewage appeared to hold similar bacterial communities, it was necessary to confirm if similar wastewaters induce a similar bacterial community. To answer this question, analysis of T-RFs for activated sludges, taken from another 12 domestic WTPs, was conducted by using a 6­FAM$^{TM}$-Iabeled primer and an automated DNA sequencer for higher sensitivity. Among 12 samples, it was again found that T-RF profiles of activated sludges from Yongin, Sungnam, Suwon, and Tancheon domestic WTPs in Kyonggi-do were very similar with each other. On the other hand, T-RF profiles of activated sludges from Shihwa and Ansan WTPs were quite different from each other. It was thought that this deviation was caused by wastewaters, since Ansan and Shihwa WTPs receive both domestic and industrial wastewaters. From these results, it was tentatively concluded that similar bacterial communities might be developed in activated sludges, if WTPs treat similar wastewaters.

• 한국해양학회지:바다
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• 제12권3호
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• pp.200-210
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• 2007

아산만 식물플랑크톤의 하계 종조성 변화를 조사하기 위하여 2006년 6월, 7월, 8월에 매달 두 번씩 실시하였으며 조사 정점은 공간적 분포를 나타내는 5개 정점을 선정하여 조사하였다. 6월과 7월에 염분은 내만에서 감소하는 경향을 보였다. 영양염은 6월과 7월에 높았으나 8월에 낮은 경향을 보였다. 2006년 여름철 아산만에서 식물플랑크톤 군집은 규조류(62.8%), 와편모조류(17.3%), 은편모조류(14.8%), 유글레나류(1.0%), 남조류(0.9%), 녹조류(0.4%) 등 6종과 확인되지 않은 종(2.8%)이 포함되었다. 6월에는 와편모조류(주요하게 Prorocentrum sp.(29.6%))가 우점 하였고 총 현존량의 43.5%를 차지하였지만 7월과 8월에는 규조류(주요하게 Leptocylindrus sp.(21.4%), Chaetoceros sp.(27.6%))가 우점 하였으며 각각 총 현존량의 69.1%, 89.9%를 차지하였다. 이와 같이 하계에도 강우에 따라 아산만 각 정점에서의 우점속의 변화가 발생하였으며 이는 강우에 따른 담수유입이 하계 아산만 식물플랑크톤 종조성변화에 영향을 미치고 있음을 암시한다.